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Citations for Millipore Sigma :
2051 - 2100 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... in polystyrene 96-well cell culture plates coated in 0.1% sterile gelatin (ES-006-B, Sigma Aldrich, St. Louis, MO). Approximately 50,000 cells were seeded for dense plating and 6,250 cells for sparse plating (1:8) ...
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bioRxiv - Cell Biology 2021Quote: ... Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma). For growth analysis ...
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bioRxiv - Developmental Biology 2022Quote: mESC carriers were maintained in Feeder Cell medium in Corning cell culture dishes precoated with 0.1% Gelatin (ES-006-B, Sigma-Aldrich), at 37 □ suppled with 5% CO2 at one atmospheric pressure ...
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bioRxiv - Immunology 2022Quote: ... Day3 stimulated primary B cells were arrested in metaphase by a 1-h treatment with 0.1 μg/ml demecolcine (Sigma, D1925), treated with 0.075 M KCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Each well was rinsed 3X with DI H2O and stained with 100 μl 0.057% w/v sulforhodamine B (SRB, Sigma-Aldrich) for 30 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... counterstained with DAPI (0.1 μg/mL) and treated with Sudan black B (0.2% weight/volume, Sigma Aldrich, St Louis, MO). DRG were cryosectioned at 12 μm ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) antibiotic-antimycotic solution (25 µg/ml amphotericin B, 10 mg/ml streptomycin, 10,000 units of penicillin (Sigma-Aldrich)) (complete DMEM ...
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bioRxiv - Neuroscience 2019Quote: ... The electrode is then backfilled with 150 mM KCl and negative-pressure used again to pull ionophore complex B (Sigma) into the tip (300-400 µm length) ...
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bioRxiv - Pathology 2022Quote: ... 2.5 μg/ml Amphotericin B [Merck], 100 U/mL penicillin, 100 μg/mL streptomycin and 250 μg/mL gentamicin [Sigma]), vortexed and stored at -80°C for later analysis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then followed the manufacturer’s protocol to extract soluble proteins by using CelLytic™ B Cell Lysis Reagent (B7435-500ml, Sigma). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... 3x105 CD19+ B cells were added to pre-existing wells containing T cells and supplemented with 200ng/mL BAFF (Sigma) in complete RPMI for 5 days ...
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bioRxiv - Physiology 2022Quote: ... however 5 μl resulted in more durability of the recording (all salts and Amphotericin-B were purchased from Sigma Aldrich.
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bioRxiv - Physiology 2019Quote: ... and homogenized in 100 μL of B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete mini, EDTA-free protease inhibitor (Sigma, 11836170001)) by sonication in a microplate-horn sonicator (Qsonica Q700 sonicator with a Misonix CL-334 microplate horn assembly) ...
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bioRxiv - Cancer Biology 2019Quote: ... Animals were treated with either an antibiotic cocktail consisting of 1mg/ml Amphoteracin B (Sigma-Aldrich, St-Louis, Missouri, USA), 25mg/ml Vancomycin (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... AG-haESCs were injected into the cytoplasm of MII oocytes in a droplet of HCZB medium containing 5 µg/ml cytochalasin B (Sigma) using a Piezo-drill micromanipulator ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Physiology 2019Quote: ... heat-polished patch pipettes with tip resistances between 3 and 5 MΩ were used and contained amphotericin B (Sigma-Aldrich) as the perforating agent ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... 2 μL of SP3 beads (1:1 ratio of Sera Mag A and Sera Mag B re-suspended in ultrapure water; Sigma) was added to dried peptides and incubated for 2 min under gentle agitation ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Control cells (not exposed to insulin or FGF21) were treated with 1 μl/well of Cytocholasin B (an inhibitor of glucose transport) (Sigma) at a final concentration of 5 μg/ml for 30 min at 37°C in order to estimate background glucose uptake ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was washed briefly in Buffer A before being resuspended by vortexing in 150 μl of Buffer B (150 mM NaCl, 50 mM pH 7.4, 1M Hexylene glycol, 1% v/v Igepal (CA-630, Sigma, UK) supplemented with protease inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... was harvested using a spatula and lysed by incubation with 0.5 mL of B-PER saturated with phenylmethylsulfonyl fluoride (PMSF, Sigma-Aldrich), supplemented with 5 mg/mL lysozyme at 28 °C for 2 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fixed embryos were washed with 3%HCl/70%EtOH solution for 3 times and stained in 1% Victoria blue B (Sigma) dissolved in 3%HCl/70%EtOH overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were rapidly resuspended on ice in 50 ml Buffer A containing 1X CelLytic B (Cat. #C8740, Sigma, MO, USA), and the suspension was incubated for 10 min on ice with occasional mixing ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Molecular Biology 2019Quote: ... and SmB(residues 1-91) [pCDFDuet-HT-B(1-91)-D3(1-75)] were constructed in a single pCDFDuet vector (Novagen) with N-terminal His(6)-tag followed by Tobacco Etch Virus (TEV ...
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bioRxiv - Systems Biology 2020Quote: ... respectively by transformation with a 1890 bp PCR amplified dig1Δ0::hyg allele and selection on hygromycin B (200 μg/ml) (Sigma Aldrich,) medium ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All polymers (60 mg) except PS(6000)-b-PEO(2000) were dissolved in 100 µL of dichloromethane (Sigma Aldrich Chemie). PS(6000)-b-PEO(2000 ...
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bioRxiv - Plant Biology 2021Quote: ... and mobile phases A and B consisted of water (Tedia, Fairfield, USA) and 0.1% formic acid and acetonitrile (Sigma-Aldrich) and 0.1% formic acid ...
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bioRxiv - Physiology 2021Quote: ... Mosquito samples infected with SFV were serially diluted in GMEM containing 2% FBS and 1X antibiotic-antimycotic solution (Final concentration: 100 U/mL penicillin, 0.1 g/mL streptomycin, 250 ng/mL amphotericin B, Sigma-Aldrich) and inoculated onto BHK-21 cells seeded the day before at 1.5 × 105 cells/ml on 24-well plates (two replicate wells per sample) ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v) PS + 1% (v/v) B-27 + 1 μM thyroid hormone (T3) (Cat. No. T6397, Sigma) + 10 μM ALK5 inhibitor + 10 μM zinc sulfate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Biochemistry 2021Quote: ... Interleukin 3-dependent hematopoietic pro-B cell line Ba/F3 (57) were transduced with the retroviral supernatant supplemented with 8 μg/ml of polybrene (Sigma) and IL-3 ...
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bioRxiv - Microbiology 2021Quote: ... Bacteria were grown aerobically at 37 °C in Middlebrook 7H9 medium supplemented with 250 µg/mL Hygromycin B (Sigma-Aldrich) and harvested after 72 h by centrifugation followed by resuspension in ice-cold PBS containing 1 mM MgCl2 and CaCl2 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... All solutions were prepared in sterile Hank’s Balanced Salt Solution (HBSS) with 1% antibiotic/antimycotic (penicillin, streptomycin, amphotericin B; Sigma Aldrich). Control embryos were treated with HBSS and antibiotic/antimycotic alone.
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bioRxiv - Molecular Biology 2020Quote: The phosphorylation of the S1/S2 linker constructs was examined in vitro using purified cyclin B-Cdk1 (Millipore 14-450) and PKA (murine cAMP-dependent protein kinase) ...
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bioRxiv - Immunology 2021Quote: ... 0.5 x 106 cells from each site were first activated using 5 µg/ml Cytochalasin B (C6762, Sigma-Aldrich, USA) for 5 mins and 5 µM fMLP (F3506 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2µL of SP3 beads (1:1 ratio of Sera Mag A and Sera Mag B re-suspended in ultrapure water; Sigma) were added to dried peptides and incubated for 2min under gentle agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... were routinely cultured at 37°C and 5% CO2 on dishes coated with 0.1% gelatin type B (Sigma Cat3G9391-100G) in GMEM (Sigma ...
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bioRxiv - Genetics 2022Quote: ... In vitro matured oocytes were enucleated by gently aspirating the first polar body and adjacent cytoplasm with glass pipette in manipulation medium supplemented with 6.7 µg/mL cytochalasin B (Sigma, C6762). The donor cell was subsequently injected into the perivitelline space ...
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bioRxiv - Neuroscience 2022Quote: ... To quench autofluorescence of lipofuscin the sections were washed 5 min in PBS and incubated with 0.1% w/w Sudan Black B (199664, Sigma-Aldrich) diluted in 70% ethanol ...
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bioRxiv - Bioengineering 2022Quote: ... 7 kDa:17 kDa) and Poly(D,L-lactide-glycolide) 50:50-b-PEG (10kDa PLGA, 2 kDa PEG) were purchased from Sigma.
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bioRxiv - Microbiology 2022Quote: ... which were plated a day before in D10 supplemented with 2.5 μg/ml of amphotericin B (Sigma, Cat. No. A2942) (which increases viral titers as shown in Figure S1B) ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to Ngn2-medium (Table 1) and cytosine b-D-arabinofuranoside (Ara-C) (2 μM; Sigma, C1768) was added once to remove proliferating cells from the culture ...
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bioRxiv - Cell Biology 2022Quote: ... At least one well on each plate served as a negative control by addition of 25μM cytochalasin B (Sigma Aldrich) to block all transporter-mediated glucose uptake ...
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bioRxiv - Bioengineering 2024Quote: ... Solvents A and B were composed of 50 mM formic acid adjusted to 4.4 pH with ammonia solution and acetonitrile (Sigma-Aldrich Acetonitrile E CHROMASOLV for HPLC ...
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Developmental Biology 2023Quote: ... the oocytes were transferred to medium supplemented with 250 μM of dbcAMP and 10 μM of cytochalasin B (Sigma-Aldrich). Thereafter ...