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Citations for Millipore Sigma :
2151 - 2200 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... passed through a 0.450□μm filter and added to ATG2A/B DKO cells in the presence of 10 μg/ml polybrene (Sigma, H9268-5G). Transduced cells were selected by addition of 1 μg/ml Puromycin (iTAP and pHAGE-nTAP-mCherry ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2019Quote: ... cells were plated onto selective Lysogeny broth (LB)-agar plates supplemented with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) (no. I6758; Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were then transferred to a drop of KSOM + 10mM SrCl2 + 4mM EGTA + 5μg/mL cytochalasin B (Sigma 255521, E8145, C6762) under mineral oil and incubated for one hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... ICS was performed by incubating cells in 30 μl of antibodies against cytokines and Granzyme B diluted in a Saponin (Sigma-Aldrich) 0.5% W/V solution ...
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bioRxiv - Immunology 2021Quote: ... After washing the B cells with cold PBS and resuspending them in 50 µl cold PBS with 1% bovine serum albumin (BSA) (Sigma #A7979), the cells were first stained with 40 nM of decoy tetramer (10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Relative fold change in DCF signal was acquired by fluorescence spectroscopy and normalised to cell density quantified by 0.04% sulforhodamine B (Sigma-Aldrich, 230162) solublised in 10 mM Trizma (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cortical vascular network was visualized via multiphoton microscopy through the injection of fluorescently conjugated dextran into the blood stream (Rhodamine B, 20 mg/ml, Sigma R9379). The pulsatility of individual vessels was determined by positioning linescans orthogonal to the vessel’s axis ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... we used rabbit polyclonal anti-AFG3L1 (Koppen et al, 2007) (Figure 1A, B and Figure S1C) and rabbit polyclonal anti-FLAG (#F7425 from Sigma-Aldrich) (Figure 3 ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... membrane depolarisation and permeabilisation was achieved by 15 min incubation in the presence of pore-forming antibiotic Polymyxin B (10 µg/ml; Sigma Aldrich), and nucleoid staining was achieved by 15 min incubation with 500 ng/ml DAPI (Severn Biotech) ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus was generated with pLentiCMV-mitoLbNOX as described above and 143B cells were infected and then selected in 150 µg/mL Hygromycin B (Sigma, H7772) for four days.
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bioRxiv - Plant Biology 2022Quote: ... Roots were sliced and placed in a petri dish (Falcon 351008) holding one 70 μm strainer (Falcon 352350) with 7 ml buffer B (cellulase 15 g l−1, (Sigma #C1794) and pectolyase 1 g l−1 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were finally washed with wash buffer B and mounted with Duolink in situ mounting medium with DAPI (DUO82040, Sigma Aldrich). Cells were imaged with Leica TCS SP8 microscope using plan apochromat 40X/1.3 N.A oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
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bioRxiv - Physiology 2021Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatin type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Physiology 2020Quote: ... Free radioligand was separated from bound radioligand by filtration through Whatman® glass microfiber GF/B 25 mm filters (Sigma-Aldrich). Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by ...
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bioRxiv - Developmental Biology 2022Quote: ... 6 dpf zebrafish larvae were placed for a few hours in water containing 10 mM BDM (2.3-butanedione monoxime: Sigma, B-0753) as previously described (Miyasaka et al. ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... were added to the cell culture medium. DLD1 and DLD1 KO FBXW7 (B. Vogelstein, Johns Hopkins University, Baltimore MD, USA) were cultivated in RPMI1640 (Sigma R8758). All media were supplemented with 10% FBS (Gibco 10270-106 ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were washed with 1mL of 1xPBS prior to adding 4µg/mL of cytokinesis block cytochalasin B (Sigma, C2743-200UL) for 24 hours ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Microbiology 2019Quote: We performed ex vivo tissue imaging of intestinal bacterial GUS activity through quantification of fluorescence following hydrolysis of fluorescein di-b-D-glucuronide (FDGlcU, Sigma-Aldrich) to fluorescein (65) ...
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bioRxiv - Pathology 2020Quote: ... 3D-EHTs were transferred from the agarose molds to 24-well tissue culture dishes containing 3D-EHT media (RPMI-1640 media supplemented with B-27 and 5 mg/mL aminocaproic acid [Sigma-Aldrich] ...
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bioRxiv - Immunology 2020Quote: ... the cells were observed for fluorescence through expression of reporter gene mTagBFP and were subjected to seven days of positive selection by adding 1 mg/mL hygromycin B (Sigma-Aldrich) in complete RPMI medium ...
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bioRxiv - Biophysics 2021Quote: ... Au-micellar solution was obtained first by dissolving a diblock copolymer polystyrene-b-poly(2-vinylpyridine) (Polymer Source) in o–xylene and second by loading with HAuCl4 · 3H2O (Sigma Aldrich) according to the specific parameter L = n[HAuCl4]/n[P2VP] ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated with staining solution (3.5 µl/ml nitro blue tetrazolium, N-6876 Sigma; 3.5 µl/ml BCIP, B-8503). After staining for 1-2 h (depending on the probe) ...
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bioRxiv - Cell Biology 2020Quote: ... This parental cell line was then nucleofected with pTcINDEX-GW TcATAT-HA construct following a similar protocol and transgenic parasites were obtained after 4 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma Aldrich).
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bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma Aldrich, St. Louis, MO, USA)5 ...
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bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...
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bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
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bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
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bioRxiv - Cell Biology 2021Quote: ... The tissue was homogenized 20 times with pestle A and 20 times with pestle B in 2 ml of ice-cold nuclei EZ lysis buffer (NUC101-1KT, Sigma-Aldrich). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Tetraploid DLD1 clones were generated by treating diploid cells with 1.5 μg/mL dihydrocytochalasin B (DCB; Sigma Aldrich, St. Louis, MO) for 20 hrs to induce cytokinesis failure ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of 2.0 mg/mL stock solution bovine serum albumin (BSA) from fraction V powder (Sigma B-4287 or equivalent), 1,000 nM primers (forward and reverse) ...
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted], cyclosporin A [Sigma-Aldrich, cat ...
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bioRxiv - Plant Biology 2022Quote: ... pERF109::GFP/coi1-2 was obtained through crossing followed by selection of homozygous plants on selective ½ MS medium containing 15 µg ml-1 hygromycin B (Melford Laboratories Ltd.) and 20 µg ml-1 MeJA (Sigma-Aldrich). Arabidopsis plants were vertically grown in sterile conditions on modified Knop medium (Sijmons et al. ...
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bioRxiv - Microbiology 2022Quote: ... 100-200 μl culture aliquots were transferred to 2 ml Eppendorf tubes with perforated lids followed by addition of 7 μM (10 μg/ml) polymyxin B (Sigma-Aldrich) or 31 μM (30 μg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Biophysics 2022Quote: ... The inhomogeneity of the TIRF illumination were corrected using the images from the solution of rhodamine B (561 nm excitation, from Sigma Aldrich) and 3,3’-diethylthiadicarbocyanine iodide (640 nm excitation ...
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bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... Sterile stocks of polymyxin B sulphate (PMB; Cat# 86-40302, Betapharma, Shanghai, China) were prepared in Milli-Q water (Millipore, USA) with filter sterilization using a 0.22-µm syringe filter (Sartorius ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Pellet was resuspended into fresh buffer B and DNA was extracted using phenol-chloroform-isoamyl acid 25/24/1 (#77617; Sigma-Aldrich). After quantification with NanodropOne (Thermo-Fischer Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... bovine trachea, average molecular mass 28 kDa26) and Dermatan Sulfate (C3788, DS/CS-B, porcine intestinal mucosa) were purchased from Sigma-Aldrich. Chondroitin Sulfate D (400676 ...