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Citations for Millipore Sigma :
1951 - 2000 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Transformants were selected in medium lacking uridine or uracil or in the presence of 150 μg/ml hygromycin B (Sangon) or 0.1 μg/ml pyrithiamine (Sigma). For the recycling usage of the selectable marker pyr4 ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Neuroscience 2020Quote: ... The human brain slices were subjected to immunofluorescence staining and the autofluorescence signal was blocked by 0.1% (w/v) Sudan Black B (199664, Sigma) in 70% ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Overnight cultures were diluted 1:100 in prewarmed LB broth containing 0.4 mM IPTG and 20 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Physiology 2021Quote: ... For measuring [K+] they were filled at the tips with Potassium Ionophore I-Cocktail B (5% valinomycin; Sigma-Aldrich) and back-filled with 500 mM KCl (Rodgers et al. ...
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bioRxiv - Microbiology 2020Quote: ... we tested their susceptibility to four antifungal drug classes: a) Polyenes: Amphotericin B (AMB, Sigma-Aldrich Quimica, Madrid, Spain); b ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Blebbing was induced by addition of either 1.7 µM (interphase) or 2.4 µM (prometaphase) of Latrunculin B (Sigma Aldrich), immediately followed by shaking on a horizontal benchtop shaker for 15 min at room temperature to detach the blebs from the cells ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed twice in DPBS then simultaneously blocked and permeabilized with B/P buffer [DPBS with 0.1% Tween 20 (P2287; Sigma), 0.1M glycine (161-0718 ...
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bioRxiv - Neuroscience 2022Quote: ... bound and free ligands were separated by rapid filtration over Whatman brand Grade GF/B glass filters (Sigma-Aldrich) using a sampling vacuum manifold (MilliporeSigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed cells were resuspended in pre-warmed (37°C) DMEM supplemented with 10 μg/ml cytochalasin B (Sigma-Aldrich) at a concentration of around 5×106 cells/mL DMEM and incubated at 37°C for at least 30 minutes ...
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bioRxiv - Biophysics 2022Quote: Inhibition of actin polymerization on NIH-3T3 cells is performed with latrunculin B (LatB, Cat. No. L5288; Sigma-Aldrich, St ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.2% Triton X-100 and then counterstained with DAPI and phalloidin tetramethyl-rhodamine B isothiocyanate (TRITC) (Millipore). Fifteen-image Z-stacks were acquired by SP8 confocal with a 63× oil-immersion objective and merged with Fiji software (http://fiji.sc/).
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bioRxiv - Neuroscience 2020Quote: ... We verified primary motor neuron identity in a small subset of recordings from eYFP-expressing cells in Tg(mnx1:GAL4;UAS:ChR2(H134R)-eYFP) larvae by adding 0.025% sulforhodamine-B acid chloride dye in the intracellular solution (Sigma-Aldrich) and filling the neuron to reveal its morphology ...
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bioRxiv - Neuroscience 2021Quote: ... and the capillary was loaded with an 8mg/mL Rhodamine B isothiocyanate-Dextran 70kDa (R9379, Millipore Sigma, Burlington, MA) in 1x aCSF ...
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bioRxiv - Physiology 2021Quote: ... The procedure consisted of using one mM of BAPNA (Nα-benzoyl-DL-arginine-p-nitroanilide hydrochloride, Sigma B-4875) substrate dissolved 500 µL of DMSO in 50 mM Tris-HCl buffer ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were resuspended with 2% FBS in PBS for downstream B cell isolation or 10% Dimethyl sulfoxide (Sigma-Aldrich) in FBS for further preservation.
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bioRxiv - Neuroscience 2022Quote: ... mice were put into deep anesthesia with isoflurane and 50 uL of Rhodamine B isothiocyanate-Dextran (70 kDa, 2.5% wt:vol, Sigma-Aldrich) was injected retro-orbitally to label the vessel lumen ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Cell Biology 2019Quote: ... gel that was laced or not laced with 1mg/mL of 70,000 MW Rhodamine B isothiocyanate-Dextran (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... cell-attached recording pipettes were filled with standard patch solution supplemented with freshly mixed amphotericin B (0.1 mM, Sigma). Liquid junction potentials were measured (9 to 11 mV) ...
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bioRxiv - Genomics 2019Quote: ... the cells were pelleted and resuspended in 1 ml of lysis buffer B [10 mM Tris-HCl (Sigma, T2663) pH 8.0 ...
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bioRxiv - Pathology 2020Quote: ... Each 3D-EHT was casted in a mold made of 2% agarose by adding 500,000 WTC11c hiPSC-CMs and 50,000 HS27a stromal cells in a fibrin gel solution (89 μL RPMI-1640 supplemented with B-27, 5.5 μl of DMEM/F12, [Gibco], 2.5 μL of 200 mg/mL bovine fibrinogen [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2019Quote: ... aggregates were cultured in 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b) and 100 µM taurine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then washed with PBS-T four times for 5 minutes each and treated with 0.1% Sudan Black-B solution (Sigma) for 10 minutes to quench autofluorescence ...
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bioRxiv - Physiology 2021Quote: ... For [H+] measurement they were filled at the tips with Hydrogen ionophore I – cocktail B (10% tridodecylamine; Sigma-Aldrich) and back-filled with a solution (pH 6 ...
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bioRxiv - Immunology 2021Quote: ... 50×106 primary B cells were spin-infected for 90mins at 2300rpm in the presence of 16μg/ml polybrene (Millipore) with pooled whole-genome Cherry-Brie lentiviral CRISPR libraries at an MOI of 0.3 to obtain 200-fold coverage of the library ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Developmental Biology 2020Quote: ... Salivary glands were incubated with Phalloidin–Tetramethylrhodamine B isothiocyanate (Phalloidin-TRITC, Sigma, P1951, 1:300 from 20 µM stock) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated in serum-free RPMI 1640 medium supplemented with cytochalasin B (10 µmol L-1; Sigma, Switzerland) for 15 min ...
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bioRxiv - Genomics 2021Quote: ... SH agar with protoplasts were covered with fresh SH agar containing 70 μg/ml of hygromycin B (Sigma-Aldrich) or 120 μg/ml of nourseothricin (Werner Bioagents ...
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bioRxiv - Microbiology 2020Quote: ... was then loaded immediately onto the target and overlaid with 1.2 μl of a matrix consisting of 9H-Pyrido[3,4-B]indole (Norharmane) (Sigma-Aldrich) dissolved in 90:10 (vol/vol ...
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bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Retrovirus were spin transduced to activated B cells in the presence of polybrene (10 ug/ml, Sigma, Cat#H9268) with 1260 RCF for 90 minutes at temperature of 32℃ ...
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bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
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bioRxiv - Immunology 2022Quote: ... The cell density was kept between 0.2-1.0 × 106 cells/ml with viability over 95% (determined with Erythrosin B (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2022Quote: ... CTV labeled cells at a concentration of 2×106 cells per mL were stimulated in complete B cell medium supplemented with combinations of 10μg/mL of LPS from Escherichia coli O55:B5 (Sigma) or 1μg/mL CD40L (Peprotech) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were spun down again at 6000x g for 5 mins and resuspended in 250 mL of M9-B (110 mM KH2PO4, 260 mM Na2HPO4, 42.7 mM NaCl, 1 x MEM Vitamin solution (Sigma), 2 mM MgSO4 1 μM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... then complexes immunoprecipitated using the monoclonal antibody 13D11 against the B-subunit followed by Protein A Sepharose beads (Sigma). After elution with cracking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and then permeabilised at room temperature for 15 min by resuspending the pellets in PBS-B/ 0.5% Triton X-100 (Sigma). Cell pellets were rinsed with PBS-B and incubated with EdU staining buffer containing 150 mM Tris-Cl pH 8.8 ...
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bioRxiv - Microbiology 2022Quote: ... Fixed cells were washed with slow running tap water and allowed to dry at room temperature then stained with 0.4% sulphorodamine B (SRB, Sigma) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... Protein expression from hybrid lac promoters was induced by addition of isopropyl-b-D-thiogalactopyranoside (IPTG 0.25 mM; Sigma) where indicated.
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bioRxiv - Neuroscience 2022Quote: ... transfected cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B (Sigma-Aldrich #H3274). 2 days later ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The samples were incubated for 1 h and quenching of autofluorescence was thereafter performed with 0.1% Sudan Black-B (Sigma) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...