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Citations for Millipore Sigma :
2101 - 2150 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Biochemistry 2023Quote: ... Transgenic plants were selected by spraying of seedlings with BASTA solution (50 µg ml-1) or growth on sterile culture plates containing 30 µg ml-1 hygromycin B (Sigma). For the GFP constructs ...
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bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...
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bioRxiv - Developmental Biology 2023Quote: ... the oocytes were transferred to medium supplemented with 250 μM of dbcAMP and 10 μM of cytochalasin B (Sigma-Aldrich). Thereafter ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Microbiology 2024Quote: ... and pellets resuspended in 1 ml resuspension media (A+B KD Medical) supplemented with 80 μg ml-1 threonine (Sigma) for strains lacking a functional thrC locus ...
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bioRxiv - Bioengineering 2024Quote: ... Solvents A and B were composed of 50 mM formic acid adjusted to 4.4 pH with ammonia solution and acetonitrile (Sigma-Aldrich Acetonitrile E CHROMASOLV for HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... ∼50 exposed females flies of normal appearance and ∼50 unexposed controls were injected in the mesopleuron with a cocktail of rhodamine B (1.44 mg/mL, Sigma R6626) and 10 KDa dextran conjugated to Cascade Blue (20 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... and cyclic lipopeptides (aculeacin A, anidulafungin, caspofungin, fengycin, iturin A, mycosubtilin, surfactin, daptomycin, and polymyxin B) were purchased from Sigma–Aldrich Company Ltd ...
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bioRxiv - Cell Biology 2023Quote: Eggs were fertilized in MSFW and transferred after observing the first deformation into a solution of MSFW TAPS containing 5µM of latrunculin B (Sigma Aldrich) diluted from a 10mM stock solution (in DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... When two or three embryos were injected they were immediately transferred in MFSW with 5µM of Cytochalasin B (Sigma Aldrich) diluted from a 10mM stock solution in DMSO ...
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bioRxiv - Genomics 2023Quote: ... 100 U /mL penicillin and 100 μg / mL streptomycin antibacterial mixture (PEN / STREP, Biochrom # A2213) and 2.5 μg /mL of anti-fungal amphotericin B (Sigma # 2942). The mixtures of tissue pieces and cells were incubated at 37 °C in humidified atmosphere with 5% CO2 in 6-well cell culture plate format for up to 3 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... and dounced for 100 strokes in a 40-ml Dounce homogeniser (using the small clearance pestle, or the pestle B; cat. D9188, Sigma), followed by two times of centrifugation for 5 min at 600g at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: Proteins guided towards high cysteine content were transformed into and expressed in Rosetta-gami B(DE3) Competent Cells (Novagen 71137). The 1 mL and 50 mL scale protein purification protocols were otherwise followed.
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... were used as stimulants and 10 % DMSO diluted in aqua ad iniectabilia (Braun) at equal volume was added as negative control.Positive controls were 1 μg staphylococcal enterotoxin B (Sigma-Aldrich) and 0.05 μg CMV with 0.05 μg AdV per well ...
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bioRxiv - Immunology 2023Quote: ... Protein fractions containing pure Cbl-b protein as confirmed by SDS-PAGE were pooled and concentrated using 10 kDa cutoff spin columns (Millipore), and the final protein concentration determined using the NanoDrop UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of fertilized embryos with well recognized two pronuclei in a droplet of M2 medium containing 5 μg/ml cytochalasin B (CB, Sigma) using a FemtoJet microinjector (Eppendorf ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 U mL-1 penicillin, 100 U mL-1streptomycin (SLS, UK) and 2.5 μg mL-1 amphotericin B (Sigma, Germany). Penicillin and streptomycin were added to eradicate contaminants from the abattoir and typically do not affect corneal migration at this concentration (Nakamura et al. ...
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bioRxiv - Genetics 2023Quote: ... Dissociated cells were washed and cultured in standard culture media (without amphotericin B) and passaged using 0.25% trysin-EDTA (Sigma-Aldrich) every 3-5 days.
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bioRxiv - Microbiology 2023Quote: ... Infection media was same as HCT-8 culture media except that it was supplemented with 1% serum instead of 10% and antifungal compound Amphotericin B (0.625 ug/ml; Sigma A2942) was added in addition to the antibiotic solution ...
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bioRxiv - Plant Biology 2023Quote: ... was transformed with each construct by the in planta method using the surfactant Silwet L-77 (Clough and Bent, 1998) and transformants were selected on 20 mg L-1 of hygromycin B (Sigma). Three independent homozygous mono-insertional T3 lines of were selected per construct ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Neuroscience 2023Quote: ... The vascular lumen was labeled by the fluorescent dye rhodamine B-dextran (Wt∼70kD) (Sigma, Cat#R9379, 10 mg/ml) through tail intravenous injection ...
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bioRxiv - Microbiology 2023Quote: ... Whenever cells were plated for infection with spike-pseudotyped viruses (including for ACE2 and sera selections described below) cells were additionally supplemented with 2.5 µg/ml of amphotericin B (Sigma, A2942) at the time of plating ...
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bioRxiv - Immunology 2023Quote: ... B cells (seeded at 5 x 105 / mL) were cultured with 5 µg/mL LPS (Sigma-Aldrich, St Louis, MO), 10 ng/mL BAFF (AdipoGen ...
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Neuroscience 2024Quote: ... were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (5% v/v b-mercaptoethanol (M7154, Sigma), 2× Laemmli ([0.01% v/v bromophenol blue ...
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bioRxiv - Bioengineering 2024Quote: ... the ideal support bath was prepared by mixing 2% w gelatin type B from bovine bone 250 LBB8 (Rousselot Biomedical) with 0.5% gum arabic (G9752, Sigma-Aldrich) in 50% v of the total solvent (deionized water ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Pathology 2024Quote: ... for homogenization in a total volume of 100 µL of 2x B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete Mini, EDTA-free protease inhibitor (Sigma, 11836170001)) ...
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bioRxiv - Biochemistry 2024Quote: ... sdAb-expressing transgenic lines were subjected to an additional selection with 5 µg ml-1 hygromycin B (HygB) (Sigma-Aldrich). Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξ CellLytic B (Sigma-Aldrich), 1 % Triton X 100 ...
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bioRxiv - Microbiology 2024Quote: ... 10 min) and resuspended in 3-5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5xCellLytic B [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2024Quote: ... We used Rhodamine B dye (Merck Sigma) (1% v/v solution in 1X Dulbecco’s Phoshate Buffer Solution from Sigma Aldrich) as a tracer particle ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... From here the silanized glass microelectrodes were back-filled with a 100 mM KCl solution and front-filled with a K+-selective ionophore (K+ ionophore I cocktail B, Sigma). Filled electrodes then had their tips rapidly dipped in a solution of poly-vinyl chloride in tetrahydrofuran (10 mg in 3 mL ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was serially diluted 10-fold in OptiMEM containing 2% FBS and 1% antibiotic-antimycotic solution (100 U/mL penicillin, 0.1 g/mL streptomycin, 250 ng/mL amphotericin B, Sigma-Aldrich) and inoculated onto BHK-21 cells seeded the day before at 1.8 ×105 cells/mL on 12-well plates ...
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Developmental Biology 2024Quote: ... in a total volume of 100 µL of B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete Mini, EDTA-free protease inhibitor (Sigma, 11836170001)) and sonicated in a microplate-horn sonicator (Qsonica Q700 sonicator with a Misonix CL-334 microplate horn assembly) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 mM NaHCO3) and resuspended in TEMB buffer supplemented with 10% (v/v) CelLytic B cell lysis reagent (Sigma-Aldrich) and 1% protein inhibitor cocktail (100× ...
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bioRxiv - Genetics 2024Quote: ... ∼20mg pulverised frozen liver tissue was dounced ten times with pestle A and ten times with pestle B (Sigma # 8938) in 1ml hypotonic lysis buffer solution B containing 10 mg/ml BSA (Sigma # 126609) ...
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bioRxiv - Evolutionary Biology 2024Quote: We chemically lysed cell pellets in 200 µl of freshly prepared lysis buffer containing Cell lytic Buffer-B (Sigma #B7435), 100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Biophysics 2019Quote: ... and the pellet was resuspended in 4 mL/g-pellet of B-PER (ThermoScientific, cat# 78266) with 1 mM PMSF (Millipore Sigma, cat# 7110 ...