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Citations for Millipore Sigma :
1851 - 1900 of 2187 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... aggregates were cultured in 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b) and 100 µM taurine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then washed with PBS-T four times for 5 minutes each and treated with 0.1% Sudan Black-B solution (Sigma) for 10 minutes to quench autofluorescence ...
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bioRxiv - Physiology 2021Quote: ... For [H+] measurement they were filled at the tips with Hydrogen ionophore I – cocktail B (10% tridodecylamine; Sigma-Aldrich) and back-filled with a solution (pH 6 ...
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bioRxiv - Immunology 2021Quote: ... 50×106 primary B cells were spin-infected for 90mins at 2300rpm in the presence of 16μg/ml polybrene (Millipore) with pooled whole-genome Cherry-Brie lentiviral CRISPR libraries at an MOI of 0.3 to obtain 200-fold coverage of the library ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Developmental Biology 2020Quote: ... Salivary glands were incubated with Phalloidin–Tetramethylrhodamine B isothiocyanate (Phalloidin-TRITC, Sigma, P1951, 1:300 from 20 µM stock) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated in serum-free RPMI 1640 medium supplemented with cytochalasin B (10 µmol L-1; Sigma, Switzerland) for 15 min ...
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bioRxiv - Genomics 2021Quote: ... SH agar with protoplasts were covered with fresh SH agar containing 70 μg/ml of hygromycin B (Sigma-Aldrich) or 120 μg/ml of nourseothricin (Werner Bioagents ...
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bioRxiv - Microbiology 2020Quote: ... was then loaded immediately onto the target and overlaid with 1.2 μl of a matrix consisting of 9H-Pyrido[3,4-B]indole (Norharmane) (Sigma-Aldrich) dissolved in 90:10 (vol/vol ...
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bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Retrovirus were spin transduced to activated B cells in the presence of polybrene (10 ug/ml, Sigma, Cat#H9268) with 1260 RCF for 90 minutes at temperature of 32℃ ...
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bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
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bioRxiv - Immunology 2022Quote: ... The cell density was kept between 0.2-1.0 × 106 cells/ml with viability over 95% (determined with Erythrosin B (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2022Quote: ... CTV labeled cells at a concentration of 2×106 cells per mL were stimulated in complete B cell medium supplemented with combinations of 10μg/mL of LPS from Escherichia coli O55:B5 (Sigma) or 1μg/mL CD40L (Peprotech) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were spun down again at 6000x g for 5 mins and resuspended in 250 mL of M9-B (110 mM KH2PO4, 260 mM Na2HPO4, 42.7 mM NaCl, 1 x MEM Vitamin solution (Sigma), 2 mM MgSO4 1 μM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... then complexes immunoprecipitated using the monoclonal antibody 13D11 against the B-subunit followed by Protein A Sepharose beads (Sigma). After elution with cracking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and then permeabilised at room temperature for 15 min by resuspending the pellets in PBS-B/ 0.5% Triton X-100 (Sigma). Cell pellets were rinsed with PBS-B and incubated with EdU staining buffer containing 150 mM Tris-Cl pH 8.8 ...
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bioRxiv - Microbiology 2022Quote: ... Fixed cells were washed with slow running tap water and allowed to dry at room temperature then stained with 0.4% sulphorodamine B (SRB, Sigma) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... the fixed and permeabilized cells were labeled with Phalloidin-Tetramethyl rhodamine B (1/1000) (Sigma-Aldrich, St. Louis, MO) for 1 hour at room temperature and Hoesch (1X ...
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bioRxiv - Microbiology 2022Quote: ... Protein expression from hybrid lac promoters was induced by addition of isopropyl-b-D-thiogalactopyranoside (IPTG 0.25 mM; Sigma) where indicated.
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bioRxiv - Neuroscience 2022Quote: ... transfected cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B (Sigma-Aldrich #H3274). 2 days later ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from B.1 viral particles was isolated from Vero cell supernatant and digested by P1 nuclease (Sigma, N8630) followed by treatment with phosphatase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... cultures are fed with melanocyte priming media (MPM) (media B with 10ng/ml BMP4 and 100ng/ml EDN3 (Sigma)) to bias the cranial NCCs towards a melanocyte fate ...
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bioRxiv - Cancer Biology 2023Quote: ... Rat anti-Ago2 (cat no. SAB4200085) and goat anti-Apolipoprotein B (cat no. AB742) were purchased from Millipore Sigma. Rabbit anti-CD63 (cat no ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For preliminary calibration, the injection solution was filtered (0.22 μm filter, Millex) after incorporation of 128μM sulforhodamine B (Sigma-Aldrich) to visualize the extent of diffusion under fluorescence ...
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bioRxiv - Cancer Biology 2023Quote: ... A lysis buffer supplemented with phosphatase inhibitor cocktails (BioTools, B15001A/B) and a protease inhibitor (Millipore Sigma, P2714-1BTL) was used to lyse the cells ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mg/mL streptomycin and 0.25 µg/mL amphotericin B (antibiotic antimycotic solution, Sigma Aldrich, St. Louis, MO, USA). Voltage clamp recordings were performed after 1-2 days of expression.
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bioRxiv - Microbiology 2023Quote: ... Dispersin B was obtained from Kane Biotech (Winnipeg MB, Canada). Deoxyribonuclease I (Catalog No. DN25) was from Sigma-Aldrich.
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and solubilized in B-PER solution (Pierce) supplemented with 1/100 (v/v) of bacterial protease inhibitor cocktail (Sigma), 1 mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... musculus embryonic stem cells (mESC E14tg2A) were cultured under feeder-free conditions using 0.1% Gelatine (Millipore, #ES-006-B) coated plates (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: ... Mobile phase A and B were 10 mM ammonium formate (Sigma-Aldrich, >99/0%, LC-MS grade, 70221-100GF), pH 10 and 20% 10mM ammonium formate (pH 10)/80% methanol (Optima LC/MS grade ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then transferred to another microcentrifuge tube and supplemented with 6.187% fresh B-mercaptoethanol (Sigma-Aldrich, M3148). Samples were then subjected to electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in Tris A (0.1M TB in 0.25% Triton-X 100) and Tris B (Tris A and 1% bovine serum albumin; Millipore Sigma) for 10 min each and then incubated for 30 min in 5% normal goat serum diluted in Tris B (blocking solution) ...
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bioRxiv - Microbiology 2023Quote: ... and mutants were selected for on osmotically stabilized minimal medium (GMM plus 1.2 M sorbitol) containing 200 µg/ml hygromycin B (Sigma). Protoplasts were generated using lysing enzyme and transformed as previously described ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reconstituted visual pigments were extracted using 1% n-dodecyl b–D-maltoside (DDM) (Sigma-Aldrich, Saint Louis, MO) in 10 ml of extraction buffer (pH 6.7 ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The samples were incubated for 1 h and quenching of autofluorescence was thereafter performed with 0.1% Sudan Black-B (Sigma) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was detected by incubation of fixed and permeabilized cells with 0.25 μg/ml phalloidin tetramethylrhodamine B isothiocyanate (phalloidin-TRITC, Sigma) in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were passaged in M9 salt medium (Difco) with 2% sodium chloride supplemented with a) casamino acids (Difco) or b) sodium caseinate (Sigma) (referred to as ‘M9-casein’ and ‘M9-CAA’ herein) ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspended in 1.2% NaCl containing antibiotic-antimycotic solution (100 units Penicillin, 100 µg/mL Streptomycin and 0.25 µg/mL Amphotericin B, Sigma-Aldrich), passed through a series of stainless-steel sieves with sequentially decreasing pore sizes (450 µm ...
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bioRxiv - Molecular Biology 2019Quote: The Burkitt lymphoma line Ramos (DSMZ #ACC603) and the murine B cell progenitor Baf3 (DSMZ #ACC300) were cultured in RPMI medium (Sigma) supplemented with 7.5% fetal calf serum ...
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bioRxiv - Biophysics 2022Quote: ... followed by buffer exchange (5 cycles of concentration to 0.5 ml volume and dilution with 5 ml buffer B) using a 3 kDa MWCO Amicon Ultra-15 centrifugal ultrafilter (Millipore), and the purified Alexa647-CAP was stored in aliquots at −80°C.
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.