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Citations for Millipore Sigma :
2251 - 2300 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and homogenized with a 2 ml dounce grinder for 5 strokes with A motor and at least 20 strokes with B motor (Sigma-Aldrich D8938). The lysates were filtered through 100 µm and 50 µm cell strainers (CellTrics filters 04-004-2328 ...
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bioRxiv - Systems Biology 2019Quote: ... was grown with 1 million seeded cells on 75 cm2 tissue culture flasks with vented caps (Falcon 353110) gelatinized with EmbryoMax 0.1% Gelatin Solution (Millipore ES-006-B) for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960 ...
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bioRxiv - Cell Biology 2021Quote: ... was performed for 5-7 minutes at a rate of 5ml/minute followed by buffer B (buffer A minus EDTA, with 35mM CaCl2 and Liberase TM, Sigma-Aldrich, 5401127001) for 5-7 minutes at a rate of 5ml/minute ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... 129/Ola isogenic background) and all clones derived from these were cultured under feeder-free conditions in 0.1% gelatin (Sigma ES- 006-B) coated dishes (Falcon ...
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bioRxiv - Immunology 2020Quote: Tyrosine hydroxylase activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalogue number: 18250), 30 min before activation with anti-IgM/CpG.
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bioRxiv - Immunology 2021Quote: ... product number:40592-V27H-B) diluted in Dulbecco’s PBS containing 0.05% Tween 20 and 0.5% fish gelatine (Sigma Aldrich, St. Louis, MO, USA) (assay buffer) ...
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bioRxiv - Biochemistry 2022Quote: ... with 10 mM ammonium formate (solvent A, Fluka, #17843) and 10 mM ammonium formate in 90% acetonitrile (solvent B, EMD Millipore, #AX0145) adjusted to pH 10 using ammonium solution (EMD Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B and 2 µg/mL of doxycycline hyclate (Sigma Aldrich #D9891). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... NIM contains 1:1 mixture of Medium A and Medium B medium (referred to as neural maintenance medium (NMM)) supplemented with 1 μM dorsomorphin (Sigma Aldrich; P5499) and 10 μM SB431542 (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Following staining, spheroids were washed 3x with CSB, washed with B/PBS (1% BSA, PBS) and stained with Hoechst (Sigma #14533-100mg) 5 mg/mL stock diluted 1:1000 in B/PBS for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... B-023), and heptadecanoic acid (as an internal standard, IS, purity ≥ 98%; lot no.H3500) were purchased from Sigma-Aldrich (Saint Louis, USA). O-methyl hydroxylamine hydrochloride (purity ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5 µl of each dilution was inoculated onto THY or THY-Tween plates containing or not 20 µg.ml-1 polymyxin B (Sigma-Aldrich, Ref. 81271). Plates were incubated at 37 °C for approximately 24 h and photographed ...
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bioRxiv - Biochemistry 2023Quote: Human thyroid cancer B-CPAP (ACC 273, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) and 8505C cells (Sigma No. 94090184) were cultured in the standard conditions in RPMI 1640 and DMEM/F12 HAM ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was homogenized in a glass Dounce tissue grinder (25 times with pestle A, 25 times with pestle B; Sigma, Cat#D8938) in 2mL ice-cold EZ Nuclei Lysis Buffer (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... The staining solution was composed of culture medium containing the red dye Erythrosin B (0.1% w/v; Sigma-Aldrich; product number: 200964). The staining solution was left to perfuse or diffuse tissue constructs following gravity ...
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bioRxiv - Microbiology 2023Quote: Highly motile infectious stage larvae (DiL3) retrieved from mosquitoes were washed in RPMI 1640 with 1% penicillin-streptomycin and 1% amphotericin B (Sigma-Aldrich, UK), and injected subcutaneously into the flank of male NSG/NXG or RAG2ɣc mice at a density of 200 DiL3 per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5X antibiotic/antimycotic solution (final concentrations 50 U/mL penicillin, 0.05 mg/mL streptomycin, 0.125 ug/mL amphotericin B; Sigma-Aldrich, St. Louis, MO) at 37 °C and 5% carbon dioxide (CO2) ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 µg/ml gentamicin) then dissociated in 0.2 ml of CMF-B plus 1 mg/ml collagenase (Sigma Aldrich; C5138-1G) per animal (e.g. ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were embedded in Durcupan resin (20 g component A, 20 g component B, 0.6 g component C, 0.4 g component D; Sigma Canada, Toronto, cat# 44610) overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The non-focal females carried the scarlet mutation (a recessive eye colour marker) and were reared from first instar larvae on SYA containing 1400 ppm Sudan Black B dye (Sigma-Aldrich #199664) dissolved in corn oil (Mazola ...
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bioRxiv - Immunology 2024Quote: ... single GC B cells were sorted into wells and 150 μl of supplemented OptiMEM along with 30 μg/ml LPS (Sigma-Aldrich, #L6511) and 4 ng/ml IL-4 (Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: MEF cells are grown for at least 24 hours and treated with 5μg/ml of Cytochalasin B (Millipore-Sigma, catalog no. 250225) in complete culture medium for 1 hour and fixed with 4% paraformaldehyde ...
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bioRxiv - Biophysics 2024Quote: MEF cells are grown for at least 24 hours and treated with 5μg/ml of Cytochalasin B (Millipore-Sigma, catalog no. 250225) in complete culture medium for 1 hour and fixed with 4% paraformaldehyde ...
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bioRxiv - Molecular Biology 2024Quote: ... 129/Ola isogenic background) and all clones derived from these were cultured under feeder-free conditions in 0.1% gelatin (Sigma ES-006-B) coated dishes (Falcon ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Immunology 2021Quote: ... and 2-mercaptoethanol at 37 °C with 5% CO2. Subsequent to isolation B cells were stimulated with 10 μg/ml lipopolysaccharide (LPS) (Catalog no. L2887) from Sigma-Aldrich (Oakville, ON). For co-culture with MSCs ...
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bioRxiv - Cell Biology 2019Quote: ... 1:5000), mouse α-tubulin (B-5-1-2, 1:10000) and mouse α-Vinculin (hVIN-1, 1:5000) were obtained from Sigma-Aldrich. Rabbit α-Cyclin B1 has been described before [46] ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and lysed for 15 mins at 37 °C in 4 mL of buffer containing 0.5X CelLytic B (Sigma-Aldrich, St. Louis, USA), 150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... from malaria-experienced children and adults were thawed in a water bath at 37°C and immediately mixed with pre-warmed thawing medium (IMDM Glutamax (Thermo #31980030) supplemented with 10% heat-inactivated fetal bovine serum (FBS) of US origin (Sigma #TMS-013-B) and 33 U/ml universal nuclease (Thermo #88700) ...
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bioRxiv - Cell Biology 2021Quote: ... Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402) antibody (Cell signalling #260; 1:150) and Phalloidin–Tetramethylrhodamine B isothiocyanate (Sigma P1951, 1:500). Anti-rabbit Alexa 405 and FITC (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained neurovascular units ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen, Paisley, Scotland], and 10 μg/mL polymyxin B [Sigma, St Louis, USA]) either alone or in the presence of circulating exosome from Wt or Arrb2-/- mice ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% glycerol, 1% Triton X-100, 10 µM MgCl2), supplemented with phosphatase inhibitor (Bio tool, B15001A/B) and protease inhibitor (Millipore Sigma, P2714-1BTL) cocktails ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Immunology 2021Quote: ... Subsequent to isolation B cells were stimulated or not with 10 µg/ml LPS (Catalog no. L2887) obtained from Sigma-Aldrich (Oakville, ON). Unless otherwise indicated ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B) (Chromasolv LC–MS grade; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... Imiquimod-stimulated B cells were treated simultaneously with or without the XBP1 inhibitor 4μ8c [24] (20 μM, Sigma-Aldrich, St. Louis, Missouri, USA) dissolved in DMSO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Activation of primary B-cells was induced by the addition of 10 μg/ml LPS (from E. coli, O111:B4, Sigma-Aldrich, Cat#L4391). Ramos cells were authenticated by Eurofins Genomics (Ebersberg ...
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bioRxiv - Cancer Biology 2024Quote: ... medium was changed to serum and glucose-free medium containing BKIDC at 20 µM or cytochalasin B (CytB, C6762 Sigma-Aldrich, Cat# C6762) at 50 µM for 30 min prior to the experiment ...
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bioRxiv - Genomics 2023Quote: ... used for spike-in controls for ChIPs of TWIST1 depletions were cultured in Complete ES Cell Medium with 15% FBS (Millipore, ES-101-B), 25 ng/ml bFGF ...
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bioRxiv - Physiology 2023Quote: ... Glp1rOBKORD RD and Glp1rOBhM3Dq WD mice in which OB Glp1r-expressing neurons were chemogenetically activated by IP injections of Salvinorin B (SALB; Sigma-Aldrich; 10mg/kg) or Clozapine N-oxide (CNO ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ml of differentiation medium (10% FBS (Millipore, Cat.No. ES-009-B, 1X L-Glutamine (Thermo Fisher, Cat.No.25030081, 1X Non-Essential AA, Millipore, Cat.No. TMS-001-C), 0.15 mM 2-Mercaptoethanol (Millipore ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained enriched neurovascular fractions ...
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bioRxiv - Neuroscience 2023Quote: The low glucose starvation medium was composed of 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b), N2 supplement (GIBCO ...