Labshake search
Citations for Millipore Sigma :
1901 - 1950 of 2187 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... The stable cell lines of DLD1-/- BRCA2 deficient cells expressing BRCA2 WT or variants of interest generated in this study were cultured in growth media containing 0.1 mg/ml hygromycin B and 1 mg/ml G418 (Sigma-Aldrich). All cells were cultured at 37°C with 5% CO2 in a humidified incubator and all cell lines used in this study have been regularly tested negatively for mycoplasma contamination.
-
bioRxiv - Microbiology 2019Quote: ... and soaking in 10x antibiotic-antimycotic solution (final concentration: 1,000 units/ml penicillin, 1 mg/ml streptomycin and 2.5 µg/ml amphotericin B, Sigma Aldrich, UK) for 3h at 25 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... 1690–1937) were amplified by PCR from the full-length cDNA and sub-cloned into pET28-a (+) or pET28-b (+) (Novagen). Human Nup58FG-N (aa ...
-
bioRxiv - Microbiology 2019Quote: ... A20 and A20.γHV68 B cells were treated with vehicle (untreated) or stimulated with 12-O-tetradecanolphrobol-13-actate (TPA) 20 ng/ml (Sigma) (in DMSO ...
-
bioRxiv - Bioengineering 2019Quote: ... and 3T3-Swiss cells were incubated in PBS containing 0.2 μg ml−1 tetramethylrhodamine B isothiocyanate (TRITC)-conjugated phalloidin (P1951; Sigma-Aldrich), 2 μg ml−1 4’,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Microbiology 2019Quote: ... Crystals were soaked in stepwise fashion to the reservoir solution B containing 18%(v/v) (2R, 3R)-(−)-2,3-butanediol (Sigma-Aldrich) and cooled in liquid nitrogen ...
-
bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
-
bioRxiv - Bioengineering 2019Quote: The ab-and adsorption of small molecules by the membranes was quantified by immerging them in 10µM rhodamine B (Sigma-Aldrich) in PBS for 2h at 37°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
-
bioRxiv - Developmental Biology 2020Quote: ... and the individual primary mature follicles were separated from the ovaries and transferred to glass scintillation vials containing Rhodamine B (Sigma) at a 1.0 mM final concentration in water ...
-
bioRxiv - Cell Biology 2021Quote: ... stimulated B cells were cultured in the presence of phorbol 12-myristate 13-acetate (PMA, 50 ng/ml; Sigma-Aldrich), ionomycin (1 μg/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... was dissolved in DMSO to a concentration of 50 mg/ml and was further diluted 1:10 in 15% hydroxypropyl b-cyclodextrin (Sigma) (w/v in PBS 7.4) ...
-
bioRxiv - Cell Biology 2021Quote: ... splenic B-cells from MD4 mice were pretreated or not with 5 μM of the Src kinase inhibitor PP2 (Millipore) (Cheng et al ...
-
bioRxiv - Cancer Biology 2020Quote: ... Animals were treated with either an antibiotic cocktail consisting of 1mg/ml Amphoteracin B (Sigma-Aldrich, St-Louis, Missouri, USA), 25mg/ml Vancomycin (Sigma) ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and the cell pellets were chemically lysed using 0.4 mL lysis buffer (CelLytic B cell lysis reagent (Sigma-Aldrich #B7310), 0.1 mg lysozyme ...
-
bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
-
bioRxiv - Microbiology 2021Quote: ... The transposon library was inoculated into 50 ml LB medium with and without 0.2 μg/ml polymyxin B (Sigma Aldrich), in duplicate ...
-
bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... in B cell media in B cell media (DMEM high glucose/Glutamax from ThermoFisher Scientific supplemented with 10% fetal bovine serum F7524 from Sigma, 100 U/mL Penicillin and 100 µg/mL Streptomycin from Life Technologies ...
-
bioRxiv - Plant Biology 2021Quote: Purified fractions containing the unprocessed form of cardosin B were concentrated 10X using amicon ultra-15 (3kDa MWCO) (Millipore, USA). For cardosin B processing assay ...
-
bioRxiv - Biophysics 2020Quote: change of medium containing syringe to apply control (CO2-charged medium) or treatment (1 μg/mL cytochalasin B, Sigma-Aldrich) medium for 1000s at a flow rate 30 μl/min ...
-
bioRxiv - Cell Biology 2021Quote: ... in polystyrene 96-well cell culture plates coated in 0.1% sterile gelatin (ES-006-B, Sigma Aldrich, St. Louis, MO). Approximately 50,000 cells were seeded for dense plating and 6,250 cells for sparse plating (1:8) ...
-
bioRxiv - Cell Biology 2021Quote: ... Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma). For growth analysis ...
-
bioRxiv - Developmental Biology 2022Quote: mESC carriers were maintained in Feeder Cell medium in Corning cell culture dishes precoated with 0.1% Gelatin (ES-006-B, Sigma-Aldrich), at 37 □ suppled with 5% CO2 at one atmospheric pressure ...
-
bioRxiv - Immunology 2022Quote: ... Day3 stimulated primary B cells were arrested in metaphase by a 1-h treatment with 0.1 μg/ml demecolcine (Sigma, D1925), treated with 0.075 M KCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... Each well was rinsed 3X with DI H2O and stained with 100 μl 0.057% w/v sulforhodamine B (SRB, Sigma-Aldrich) for 30 minutes at room temperature in the dark ...
-
bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
-
bioRxiv - Neuroscience 2022Quote: ... counterstained with DAPI (0.1 μg/mL) and treated with Sudan black B (0.2% weight/volume, Sigma Aldrich, St Louis, MO). DRG were cryosectioned at 12 μm ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) antibiotic-antimycotic solution (25 µg/ml amphotericin B, 10 mg/ml streptomycin, 10,000 units of penicillin (Sigma-Aldrich)) (complete DMEM ...
-
bioRxiv - Neuroscience 2019Quote: ... The electrode is then backfilled with 150 mM KCl and negative-pressure used again to pull ionophore complex B (Sigma) into the tip (300-400 µm length) ...
-
bioRxiv - Pathology 2022Quote: ... 2.5 μg/ml Amphotericin B [Merck], 100 U/mL penicillin, 100 μg/mL streptomycin and 250 μg/mL gentamicin [Sigma]), vortexed and stored at -80°C for later analysis ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... We then followed the manufacturer’s protocol to extract soluble proteins by using CelLytic™ B Cell Lysis Reagent (B7435-500ml, Sigma). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... 3x105 CD19+ B cells were added to pre-existing wells containing T cells and supplemented with 200ng/mL BAFF (Sigma) in complete RPMI for 5 days ...
-
bioRxiv - Physiology 2022Quote: ... however 5 μl resulted in more durability of the recording (all salts and Amphotericin-B were purchased from Sigma Aldrich.
-
bioRxiv - Physiology 2019Quote: ... and homogenized in 100 μL of B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete mini, EDTA-free protease inhibitor (Sigma, 11836170001)) by sonication in a microplate-horn sonicator (Qsonica Q700 sonicator with a Misonix CL-334 microplate horn assembly) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Animals were treated with either an antibiotic cocktail consisting of 1mg/ml Amphoteracin B (Sigma-Aldrich, St-Louis, Missouri, USA), 25mg/ml Vancomycin (Sigma) ...
-
bioRxiv - Developmental Biology 2019Quote: ... AG-haESCs were injected into the cytoplasm of MII oocytes in a droplet of HCZB medium containing 5 µg/ml cytochalasin B (Sigma) using a Piezo-drill micromanipulator ...
-
bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
-
bioRxiv - Physiology 2019Quote: ... heat-polished patch pipettes with tip resistances between 3 and 5 MΩ were used and contained amphotericin B (Sigma-Aldrich) as the perforating agent ...
-
“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... 2 μL of SP3 beads (1:1 ratio of Sera Mag A and Sera Mag B re-suspended in ultrapure water; Sigma) was added to dried peptides and incubated for 2 min under gentle agitation ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Control cells (not exposed to insulin or FGF21) were treated with 1 μl/well of Cytocholasin B (an inhibitor of glucose transport) (Sigma) at a final concentration of 5 μg/ml for 30 min at 37°C in order to estimate background glucose uptake ...
-
bioRxiv - Cell Biology 2020Quote: ... The pellet was washed briefly in Buffer A before being resuspended by vortexing in 150 μl of Buffer B (150 mM NaCl, 50 mM pH 7.4, 1M Hexylene glycol, 1% v/v Igepal (CA-630, Sigma, UK) supplemented with protease inhibitors ...
-
bioRxiv - Microbiology 2021Quote: ... was harvested using a spatula and lysed by incubation with 0.5 mL of B-PER saturated with phenylmethylsulfonyl fluoride (PMSF, Sigma-Aldrich), supplemented with 5 mg/mL lysozyme at 28 °C for 2 h ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fixed embryos were washed with 3%HCl/70%EtOH solution for 3 times and stained in 1% Victoria blue B (Sigma) dissolved in 3%HCl/70%EtOH overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... Pellets were rapidly resuspended on ice in 50 ml Buffer A containing 1X CelLytic B (Cat. #C8740, Sigma, MO, USA), and the suspension was incubated for 10 min on ice with occasional mixing ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
-
bioRxiv - Molecular Biology 2019Quote: ... and SmB(residues 1-91) [pCDFDuet-HT-B(1-91)-D3(1-75)] were constructed in a single pCDFDuet vector (Novagen) with N-terminal His(6)-tag followed by Tobacco Etch Virus (TEV ...