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Citations for Millipore Sigma :
2051 - 2100 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The DNA test sequences for cyclization experiments (bottom PCR templates) were synthesized by Sigma Genosys (Israel) ...
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bioRxiv - Neuroscience 2023Quote: Fragments of eaat genes were PCR amplified using a JumpStart Taq polymerase (Sigma-Aldrich) and the primer pairs eaat5b_135_s 5’-GGAGCAGGAAGTCAAGTA-3’ with eaat5b_600_as 5’-GTCCGTCCCATTATCGTC-3’ and eaat7_142_s 5’-GTAATAGCAGGCACAGTGATG-3’ with eaat7_1001_as 5’-CCCAGAGCAGTGATCCAAG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was performed by LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Table S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were tested for mycoplasma using the Mycoplasma PCR detection kit (Sigma; D9307).
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bioRxiv - Cell Biology 2023Quote: ... MPP6 and NO66 were PCR cloned from plasmid DNA or cDNA into pFLAG (Sigma), pEF6 (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... Amplification of the tagmented DNA was performed using the KAPA2G Robust PCR kit (Sigma) and custom P5 and P7 indexing primers ...
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bioRxiv - Developmental Biology 2023Quote: ... The genes of interest were PCR amplified using JumpStart REDTaq ReadyMix (Sigma-Milipore, P0982) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely tested for mycoplasma with LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich) and confirmed to be mycoplasma negative ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was purified with RedExtract-N-Amp tissue PCR kit (XNAT-1000RXN, Sigma-Aldrich) using directions provided by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... These cell lines were tested for mycoplasma contamination using LookOut mycoplasma PCR detection (Sigma).
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bioRxiv - Biophysics 2024Quote: ... 45 μL of proteinase K (PK) mix (20 μg PK (Sigma Aldrich, PCR grade), 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were screened for mycoplasma every 2 months (Mycoplasma PCR Detection Kit (Sigma Aldrich) and authenticated regularly by short tandem repeat profiles at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource.
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bioRxiv - Genomics 2023Quote: ... We routinely tested cultures using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, USA) to confirm negative Mycoplasma status.
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bioRxiv - Cell Biology 2023Quote: ... Cell lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma Aldrich). Caco2 colon epithelial cells (ATCC ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was purified using Amicon Ultra-2mL 50K centrifugal filter (Millipore UFC205024) to exchange the PCR solution for 1X NEB CutSmart buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and free of mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich) or the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was used for genotyping through REDTaq PCR Reaction Mix (Sigma Aldrich). Specific primer sets were applied to detect the CMV-Cre and IL-17A transgenes ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit protocol (Sigma-R4775) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... The whole slide was then warmed for 1 min at 37°C and immediately fixed for 10 min at room temperature (RT) in a 2% formaldehyde solution (1:20 37% formaldehyde acquired from Sigma-Aldrich, Missouri, USA in 1x PBS pH 7.4). The sections were dried with isopropanol and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Indexing and amplification was done using the KAPA HiFi HotStart PCR kit (Sigma-Aldrich, KK1508) with Index oligonucleotides (sequences were adapted from Illumina).
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative (q)PCR was performed using SYBR Green Jump Start Taq Ready Mix (Sigma S4438) and Intron-spanning primer pairs (Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was purified using the High Pure PCR Product Purification Kit (11732676001 Sigma-Aldrich). Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA ...
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571) and the Reverse transcription PCR kit from Millipore Sigma (Product No. HSRT100), with specific primers were designed for each target gene (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR product were assembled into KpnI and EcoRI difgested pZDonor-AAVS1 Puromycin plasmid (Sigma-Aldrich) and ligated using the Quick Ligase Kit (New England Biolab) ...
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bioRxiv - Microbiology 2019Quote: ... Taq Polymerase and restriction enzymes for cloning and PCR methods were purchased from Sigma Aldrich. PCR cycle was as follows ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were used as a template for PCR using the KOD high fidelity polymerase (Novagen) and a mixture of 8 VH family and IgM-specific primers (listed below ...
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bioRxiv - Cell Biology 2021Quote: ... and regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned at the NdeI and XhoI restriction sites of pET30a (Novagen) to add a C-terminal 6x-his tag using the Gibson assembly cloning system ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was subsequently cloned into the BamHI-HindIII restriction sites of pETDuet (Novagen) to generate the recombinant plasmid pETDuet-DHS1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The designed promoters were obtained by either PCR (KOD Hot-Start DNA polymerase, Merck-Millipore). PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were purified using GFX PCR DNA and Gel Band Purification Kit (Sigma-Aldrich), according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were tested for mycoplasma using Lookout Mycoplasma PCR kit (Sigma-Aldrich MP0035).
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bioRxiv - Cell Biology 2022Quote: ... Regular mycoplasma testing was performed using the LookOut Mycoplasma PCR detection (Sigma, Cat. No. MP0035).
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bioRxiv - Developmental Biology 2020Quote: The four lncRNAs tested were PCR-amplified with KOD Xtreme™ DNA Hotstart Polymerase (Millipore) from their 5’ end up until the last codon of the sORF to be tested ...
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bioRxiv - Immunology 2020Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... Positive plants were further confirmed by PCR and western blot using GFP antibodies (Sigma-Aldrich). The positive T2 generation transgenic plants were evaluated in a growth chamber.
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... 2 μL of sample was added to 1x PCR Master Mix (Sigma-Aldrich, Basel, Switzerland) and the 4 degenerate primers at a final concentration of 1 μM each in a final volume of 25 μL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified fragments were purified using spin columns (GenElute TM PCR Clean-Up Kit, Sigma- Aldrich) and were checked on agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2022Quote: ... Clones were screened for N-Se loss by PCR using REDTaq ReadyMix (Sigma, R2523-100RXN) and custom primers (5’-AGCGAGACTCCGTCTAGAAA-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Genomics 2019Quote: ... We performed qRT-PCR using FastStart Universal SYBR Green Master Mix with ROX (Sigma, 4913914001) on a ViiA 7 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Genomics 2019Quote: ... Tail snips were prepared using the REDExtract-N-Amp Tissue PCR Kit (Sigma-Aldrich XNAT) and were screened for Ube3a deletion using PCR primers spanning the expected deletion site ...
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bioRxiv - Genomics 2021Quote: ... Cells tested negative for mycoplasm contamination with the LookOut Mycoplasm PCR Detection Kit (Sigma; MP0035) and Jumpstart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with SacI und SalI and cloned into pET-24a (Novagen), resulting in plasmid pET24a-OLA1-6xHIS ...
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bioRxiv - Microbiology 2019Quote: ... Digested PCR product was ligated into a modified T7 promotor-based pET33b expression vector (Novagen) that contained the super-folding variant of green fluorescent protein encoding gene with C-terminal 6xHis-tag and a modified multiple cloning site ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were selected and screened by PCR using the Expand Long Range dNTPack (Sigma), PCR buffer supplemented with 2% DMSO and the primer pairs for the 5’ region (LR 5’ fw ...
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bioRxiv - Microbiology 2022Quote: ... and were verified to be mycoplasma free by a LookOut Mycoplasma PCR Detection kit (Sigma). C ...
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bioRxiv - Bioengineering 2022Quote: ... Fragments were amplified by PCR using Q5 polymerase and assembled into a pET28a(+) vector (Novagen) via Gibson Assembly using reagents from New England Biolabs (NEB) ...