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Citations for Millipore Sigma :
1951 - 2000 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted cDNA was amplified by PCR using the Sybr Green Master Mix (Sigma), and raw threshold-cycle time (Ct ...
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bioRxiv - Molecular Biology 2021Quote: ... the TADA2b cDNA fragment was PCR amplified and cloned in pET15b vector (Novagen) using Nde I and Bam HI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma) and primers P3 and P4 ...
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bioRxiv - Molecular Biology 2019Quote: ... but tested negative for mycoplasma by PCR-based detection kit (Sigma-Aldrich D9307). Medium containing 0.5mM palmitate was prepared by first dissolving FFA-free BSA (Fisher Scientific BP9704100 ...
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bioRxiv - Biophysics 2019Quote: ... We visualized PCR products and DNA plasmids by 1 % agarose (SIGMA Cat.#A9539) gel dyed with GelRed (BIOTIUM Cat.#41003) ...
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bioRxiv - Pathology 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035). iPSC lines for the iMg were cultured by the Human Pluripotent Stem Cell Core (CHOP).
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bioRxiv - Immunology 2019Quote: ... Respective cDNA regions were PCR amplified and cloned into the pET24b vector (Novagen) for bacterial expression ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the gene encoding CAT was PCR-amplified from pACYC-Duet™-1 (Novagen) to include a 3’ flexible linker (GTSAAAG ...
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bioRxiv - Immunology 2019Quote: ... Cells were verified as mycoplasma-free using the LookOut PCR detection Kit (Sigma).
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bioRxiv - Bioengineering 2019Quote: PCR master mix: 1x KOD Xtreme Hot Start DNA Polymerase buffer (EMD Millipore), 1.2 µM (each ...
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bioRxiv - Genomics 2020Quote: ... a specific FAM digital PCR assay was designed (Sigma-Aldrich, Gillingham, Dorset, UK) targeting the promotor region of RASSF1A and five 5’-CGCG-3’ BstUI recognition sequences ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product designed for homologous recombination was transformed using lithium acetate (Sigma) as previously described (Tripp et al. ...
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bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
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bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
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bioRxiv - Microbiology 2020Quote: ... Inverse PCR using this construct was carried out with KOD polymerase (EMD Millipore) according to manufacturers instructions ...
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bioRxiv - Microbiology 2021Quote: PCR reactions were carried out with either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNA was purified with a GenElute PCR cleanup kit (Sigma-Aldrich), and concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Conventional PCR was performed using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was subsequently checked for DNA by PCR using RedTaq readymix (Sigma). RNA integrity was assessed using 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... mm9) ampli?ed by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, 11636090910). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Nectin-3 alpha was PCR amplified (KOD hot start DNA polymerase, Sigma-Aldrich) from a mouse brain cDNA library using the forward primer ...
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bioRxiv - Genetics 2019Quote: ... PCR plates were filled with a master mix for KOD Hotstart (Novagen #71086) PCR reactions containing Lister427 gDNA according to manufacturer’s specifications and primer pairs specific to each ORF were transferred into the associated well number for each plate by TECAN Freedom EVO 150 pipetting instrument ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR product was digested with NcoI and XhoI and cloned into pET28a (Novagen) in frame with a C-terminal his-tag coding sequence E ...
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bioRxiv - Cell Biology 2019Quote: ... PCR was performed using the KOD Hot Start DNA polymerase kit (Novagen, TOYOBO). The list of primers used for RT PCR is indicated on table 3.
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bioRxiv - Systems Biology 2020Quote: ... Mycoplasma was not detected with the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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Orange is the new white: taxonomic revision of Antarctic Tritonia species (Gastropoda: Nudibranchia)bioRxiv - Zoology 2020Quote: ... 3.3 μL REDExtract-N-Amp PCR ReadyMix (Sigma Aldrich, St. Louis, MO, USA), 0.3 μL of each primer ...
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bioRxiv - Molecular Biology 2021Quote: ... bL33 were PCR-amplified and cloned individually into the pET24(a) plasmid (Novagen). Similarly ...
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bioRxiv - Genomics 2019Quote: ... The libraries were PCR amplified with KAPA HiFi Hotstart Ready mix (Sigma-Aldrich) and barcoded Nextera custom primers 17 and finally size-selected (250–350 bp ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PCR products were concentrated using Amicon Ultra centrifugal filter units (Millipore) and buffer exchanged against 10 mM HEPES pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035).
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit (Sigma): 50 µl of extraction solution and 12.5 µl of tissue preparation solution was added to each embryo ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were visualized after agarose gel electrophoresis using GelRed® stain (Sigma) and a GelDoc imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: All PCR reactions were carried out using KOD Hot Start DNA polymerase (Novagen). All RT-PCRs were carried out using Takara’s PrimeScript High Fidelity RT-PCR kit (R022A).
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Mycoplasma contaminations were tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, USA). LNCaP abl were subjected to short tandem repeat (STR ...
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bioRxiv - Bioengineering 2022Quote: ... After digestion the PCR product was ligated into the pET30a vector (EMD Millipore). The resulting construct encodes EcSTH with both an N-terminal Hisx6-tag and a C-terminal FLAG-tag.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... It was generated by PCR amplification (DNA KOD Hot-start DNA Polymerase, Novagen) and cloned into the pENTRD-TOPO Gateway vector using the manufacturer’s protocol and confirmed by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were tested for mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was subcloned and inserted into a pET-24b (+) plasmid (Novagen) digested with NheI/XhoI placing the gene upstream of a sequence encoding six histidine residues ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using SYBR green PCR Jumpstart Taq ReadyMix (Sigma-Aldrich), ROX Reference Dye (ThermoFisher Scientific ...