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Citations for Millipore Sigma :
2001 - 2050 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were screened for mycoplasm using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) or Mycostrip detection kit (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mix was: 0.45 µL DMSO for PCR (Sigma; cat# D8418-50mL), 0.0045 µL SYBR Green I (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
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bioRxiv - Biophysics 2024Quote: ... sample wells (Fig. S14) were made from half-cut PCR tubes (Sigma-Aldrich) glued to the prepared slides with epoxy (Ellsworth Adhesives) ...
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bioRxiv - Microbiology 2020Quote: ... and b (chl b) were identified (RT and maxima absorption spectra) by direct injection of chl a and chl b standards (Sigma-Aldrich C-5753 and 00538, respectively) under the same HPLC conditions ...
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bioRxiv - Neuroscience 2021Quote: ... Nitrocellulose membranes were incubated in Odyssey blocking solution from Licor (30 min at RT) and later blotted with primary mouse anti-Htt MAB5492 from Millipore (12–16 h at 4 °C). Membranes were washed 3 times in PBS-Tween 0.1% and then incubated with secondary Alexa680-conjugated goat anti-mouse (Licor ...
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bioRxiv - Biochemistry 2019Quote: ... secondary antibody incubation was carried out for 1 h at RT followed by staining of the nuclei with DAPI (Sigma, Cat. No. 32670, Lot No. BCBD9022V) and mounting of the sections with ProLong Gold Antifade Mountant (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hour at RT and incubated with antibody against β-III-tubulin (mouse anti-β-III-tubulin isoform, 1:500; Millipore cat#MAB1637, RRID: AB_2210524) and antibody against GFAP (chicken anti-GFAP ...
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bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592, 1/5000; Myc-HRP, Sigma-Aldrich, A5598, 1/5000; GAPDH-HRP, Sigma-Aldrich, G9295, 1/5000). Bioluminescence emission was subsequently measured using Clarity ECL Western Blotting Substrate (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 60 ng of genomic DNA template were added to the master mix of 20 μl containing the Bio-Rad 2× ddPCR™ Supermix QX200™ EvaGreen® and the primers (SIGMA®,St. Louis, MO, USA) at the final concentration of 450-900 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were blocked with 10% FBS in PBS for 1 h at RT and incubated overnight at 4°C with a primary anti-γH2AX antibody (05-636, EMD Millipore, 1:500 in 10% FBS-PBS). Following primary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2020Quote: ... were reconstituted in ACN/IPA/H2O (65:30:5, v/v/v, 100 µL) by vortexing (2 × 30 s, RT) and transferred to an amber vial (Sigma; QSertVial™, 12 × 32 mm, 0.3 mL) for LC–MS analysis.
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bioRxiv - Biophysics 2022Quote: ... cells were washed 2x at room temperature (RT) with PBS containing calcium and magnesium pH 7.2 (PBS/Ca/Mg) (either made by hand or Sigma D1283 diluted to 1x and pH’d to 7.2) and incubated with 2 mM DSP (ChemScene CS-0068460 ...
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bioRxiv - Microbiology 2020Quote: ... and verified to be mycoplasma-free using LookOut® Mycoplasma PCR Detection Kit (Sigma). Density gradient purified Chlamydia trachomatis L2/434/Bu (ATCC VR902B ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments generated were cloned into a pET-41 Ek/LIC vector (Novagen) expressing an N-terminally GST-His6-tagged protein ...
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bioRxiv - Microbiology 2020Quote: ... The linear constructs were gel purified using a PCR cleanup kit (Sigma-Aldrich, Australia), confirmed by Sanger sequencing and concentrated to ∼500 ng/μl.
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bioRxiv - Microbiology 2021Quote: ... which was combined with 10µL of KiCqStart SYBR Green PCR Ready Mix (Millipore Sigma), 1µL of forward primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were purified using PCRu96™Plates (Merck Millipore Inc., Burlington, MA) and sequenced by an ABI 3130 (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... Q-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were tested for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma MP0035), following the manufacturer’s instructions and using the JumpStart Taq DNA polymerase
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bioRxiv - Neuroscience 2019Quote: ... The wild-type or T94M mutant ORFs were then PCR-cloned into pET28c (Novagen) for bacterial production of the proteins with an N-terminal 6× His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... The PTBP1 ORF was PCR amplified and subcloned by gibson cloning into pET21A+ (Novagen) digested by XhoI and NheI ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were separated by electrophoresis on a 2.5% agarose gel containing GelRed (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Genomics 2020Quote: ... Cultures were routinely tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, USA) to confirm negative infection status.
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bioRxiv - Microbiology 2019Quote: ... The digests were then purified with either GenElute PCR Clean-Up Kit (Sigma-Aldrich) or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination was tested monthly using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). All cells were mycoplasma-free ...
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bioRxiv - Microbiology 2021Quote: ... DNA from tail tips was isolated using Extract-N-Amp Tissue PCR kit (Sigma) and PCR genotyping undertaken as described (The Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with a commercial kit (Sigma JumpStart™ Taq ReadyMix™, P2893) and 1-μL of purified DNA ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed using the SYBR Green JumpStart Taq Ready Mix (Sigma Aldrich) on an ABI Prism 7900 system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCR reactions were carried out by KOD Hot Start DNA polymerase (Merck Millipore). PCR and Gel purification were done using Qiaquick nucleotide removal kit (qiagen ...
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bioRxiv - Genomics 2020Quote: ... we added 11 µl of KAPA2G Robust PCR master-mix (Sigma Catalogue No. KK5005), comprising 4 µl KAPA2G buffer ...
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bioRxiv - Microbiology 2020Quote: ... A second round of PCR performed with KOD Hot Start Master Mix (Millipore, 71842) supplemented with 1mM MgCl2 under the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... All inverse PCR reactions were carried out using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested negative for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma, MP0035). All cells were maintained at 37°C in a humidified incubator with 5% CO2 and were grown in media supplemented with 10% dialyzed fetal bovine serum (Sigma-Aldrich F0392) ...
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bioRxiv - Paleontology 2019Quote: ... Bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealed fragments were purified using the GenEluteTM PCR Clean-Up Kit (Sigma Aldrich, NA1020). Bi-directional precursor construct (pZ073 ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). The size of barcoded PCR products (approximately 1500 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Table S6 ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were sub-cloned into the plasmid expression vector pET-28a (Novagen) previously cut with the restriction enzymes EcoR I and Xho I ...
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bioRxiv - Bioengineering 2022Quote: ... and (ii) inlets of the hydrogel chamber were sealed using PCR tape (Sigma Aldrich). Depending on the type of analysis ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was detected by electrophoresis on agarose gel (Sigma-Aldrich, Darmstadt, Germany), using ethidium bromide (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Immunology 2022Quote: ... ORFs were synthesized as gBlocks (IDT) and assembled using PCR (KOD mastermix, Milipore Sigma) using the following primers ...