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Citations for Millipore Sigma :
1851 - 1900 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... the inserts were PCR amplified with KOD HotStart Polymerase (Novagen) and verified by Sanger sequencing.
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bioRxiv - Plant Biology 2022Quote: ... The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... and cDNA purification (Genelute PCR clean-up kit, Sigma-Aldrich) were carried out ...
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bioRxiv - Plant Biology 2024Quote: ... Diagnostic PCR was conducted using JumpStart REDTaq ReadyMix (Sigma Aldrich) with one of the following primer pairs for the T-DNA ...
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bioRxiv - Microbiology 2023Quote: ... purified with the GenElute PCR Clean-up kit (Sigma Aldrich) again ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed with KAPA SYBR® FAST (Sigma) on Rotor-Gene RG-3000 A (Corbett Research).
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bioRxiv - Microbiology 2023Quote: ... DNA sequencing of PCR products was performed by Sigma-Aldrich. PCR reactions were carried out with Taq DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and purified using the GenElute PCR cleanup kit (Sigma Aldrich). The DNA library was prepared using the Illumina DNA M preparation kit and sequenced on the Illumina NovaSeq 6000 platform at the AGRF ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cloned into the pET22b vector (Novagen) pre-digested with the NdeI and XhoI restriction enzymes (Thermo ...
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bioRxiv - Genomics 2023Quote: ... Library PCRs were supplemented with 2× SYBR dye (Sigma S9430) to monitor amplification by quantitative(q)PCR on a Roche LightCycler 480 ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR was performed with KAPA SYBR® FAST (Sigma) on a Rotor-Gene Q (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... Tissue digestion for DNA extraction and subsequent polymerase chain reaction (PCR) was performed with the prepared sample buffer from REDextract-N-Amp PCR KIT (Sigma-Aldrich, St. Louis, United States) or DreamTaq™ Hot Start Green DNA Polymerase (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: The chvI and mirA coding sequences (wild type and mutant alleles) were amplified from cloning constructs (primer sequences in Table 2) and ligated into pET-28a(+) (Novagen/ EMB Biosciences/ Millipore). Protein-expression plasmids were transformed into E.coli Top10 F’ for collection and sequence verification and then transformed into E.coli BL21/λDE3 for expression and protein purification ...
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bioRxiv - Developmental Biology 2021Quote: ... Primer sequences were sourced from publications and reported previously (Aykroyd et al., 2020) or designed using NCBI Primer Blast and produced by Sigma-Aldrich (Table S1). Only primers which produced a PCR product of the desired size ...
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bioRxiv - Plant Biology 2023Quote: The full-length coding sequences of AtCXE5 and AtCXE12 were amplified from Arabidopsis cDNA using gene-specific primers (Supplemental Table S1) and ligated into the pET28b expression vector (Novagen, Madison, WI, USA) in-frame with a N-terminal hexahistidine (His)-tag ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Cell Biology 2020Quote: ... Veeco Probes) with a spring constant of 60 pN/nm were functionalized by N1-(3-trimethoxysilylpropyl)diethylenetriamine (30 min, RT, Sigma Aldrich, 06666) followed by heating (30 min ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with 4N HCl at RT for 30 min and incubated with mouse anti-BrdU at 1:100 (B2531, Sigma, Milan, Italy) in blocking solution (0.1% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 1 hour at RT with the following antibodies diluted in blocking buffer: fibronectin (Sigma-Aldrich, St. Louis, MO) and α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2021Quote: ... RPE organoids were fixed in 4 % paraformaldehyde (AppliChem GmbH, Darmstadt, Germany) for 20 min at room temperature (RT) and subsequently equilibrated to 30 % sucrose (Sigma-Aldrich, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h at RT followed by nuclear counterstaining with DAPI (1 μg/ml in 1 x PBS; Sigma-Aldrich, Germany) for 10 min at RT ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... and 0.4% Triton X-100 in Tris-bufered saline (TBS) at room temperature (RT) for 1 h and then incubated with primary antibodies against Sftpc (AB3786, Millipore; 1:500), Pdpn (8.1.1 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times with PBS and incubated 2 h at RT in the dark with FITC-coupled α-rabbit antibodies (1:200 in BSA-PBS, Sigma Aldrich). After washing and drying ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were rinsed once with PBS and fixed for 15 min at RT with 4% PFA (Sigma−Aldrich, cat. no. 158127) in 0.1 M phosphate buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were rinsed three times with PBS and incubated 1 hour at RT with secondary antibodies and DAPI (1/10000, Sigma-Aldrich D9564) diluted in the same solution and rinsed 3 times with PBS before mounting on slides with VectaShield® Vibrance mounting medium.
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bioRxiv - Biochemistry 2022Quote: ... and a mixture of 3,3′-diaminobenzidine and urea-hydrogen peroxide (SigmaFast DAB tablets, Sigma-Aldrich, St Louis, MO, 30-60 sec, RT). The peroxidase/DAB reaction was stopped by rinsing the sections with 0.05M Tris-HCl buffer ...
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bioRxiv - Cell Biology 2022Quote: ... shaking at 60 °C) and alkylated (addition of 12 µL x 500 mM iodoacetamide, Sigma, in AB; 30 min. shaking at RT). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma, X100] in 1xPBS) and then with primary antibodies for 2-3 h at RT ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and permeabilized at RT for 20 minutes using 0.5% w/v saponin (Sigma Aldrich, Cat # 47036), 0.5% v/v Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated in 70% EtOH for 1 hour on a nutator at RT and rinsed in demi-water and stained overnight (O/N) in carmine solution (1g carmine (Sigma, cat. #C1022), 2.5 g aluminum potassium sulphate (Merck ...
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bioRxiv - Genetics 2024Quote: ... the slides were washed in 1x PBS for 5 min at RT and incubated in 0.005% Pepsin (Sigma-Aldrich, Cat# P7012-250MG) in 0.01 N HCl solution for 10 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592, 1/5000; Myc-HRP, Sigma-Aldrich, A5598 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... slides were dried in dark conditions at RT and a glass coverslip was mounted on top of each slide using Fluoromount mounting medium (Sigma Aldrich, USA). Z-stack images with a step size of ∼ 2 μm were acquired with an UPlanSApo 20× or a PlanApoN 60x objective using an inverted confo-cal microscope (FV1200 ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVECs on gelatin-coated plates were incubated with gcEVs labeled with the fluorescent dye PKH-26 (Sigma-Aldrich, 10 min at RT). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μL of each lysate sample was incubated for 1 h at RT with 10 μL of 10 mM biotin picolyl azide (in DMSO) (Sigma Aldrich 900912), 10 μL of 50 mM TCEP (in H2O) ...
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bioRxiv - Plant Biology 2023Quote: ... A 90-min incubation at RT on a tube rotator was followed by supplementation with 10 mg/mL collagenase type II (Sigma-Aldrich, USA) in Ca2-free OR2 and ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...