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Citations for Millipore Sigma :
1801 - 1850 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Primer sequences were sourced from publications and reported previously (Aykroyd et al., 2020) or designed using NCBI Primer Blast and produced by Sigma-Aldrich (Table S1). Only primers which produced a PCR product of the desired size ...
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bioRxiv - Plant Biology 2023Quote: The full-length coding sequences of AtCXE5 and AtCXE12 were amplified from Arabidopsis cDNA using gene-specific primers (Supplemental Table S1) and ligated into the pET28b expression vector (Novagen, Madison, WI, USA) in-frame with a N-terminal hexahistidine (His)-tag ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR was performed with REDTaq ReadyMix (Sigma-Aldrich, cat# R2523) and loaded on a 1% agarose gel ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR-product was amplified with the KOD polymerase (Novagen; 71086) and purified with the QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Neuroscience 2019Quote: cDNA was PCR-amplified using a JumpStart REDTaq ReadyMix (Sigma-Aldrich) and svr- ...
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bioRxiv - Cell Biology 2021Quote: ... The cap and lower third of 0.2 mL PCR tubes (Sigma) were removed to create wells for imaging extract12 ...
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bioRxiv - Microbiology 2021Quote: ... The colony PCR was performed with REDTaq ReadyMix (Sigma-Aldrich R2523) using the T7 promoter primer as forward and the T7 terminator primer as the reverse ...
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bioRxiv - Microbiology 2020Quote: ... PCR was carried out using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed with SybrGreen JumpStart Taq (Sigma-Aldrich, USA) in a RotorGene qPCR thermocycler (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplicons were ligated into the kanamycin resistant vector pET28b(+) (Novagen) using T4 ligase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... VZV ORF31 was amplified by PCR with KOD Extreme (EMD Millipore) following the manufacturer’s instructions using the oligonucleotides [31]F56625-56645/[31]R59697-59717 ...
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bioRxiv - Genetics 2022Quote: ... The PCR was performed with Thermococcus kodakaraenis (KOD) DNA polymerase (Novagen).
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich) and respective primers ...
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bioRxiv - Immunology 2020Quote: ... All PCR products were amplified using KOD DNA polymerase (EMD Millipore) and purified by gel extraction (Clontech Libraries).
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bioRxiv - Synthetic Biology 2020Quote: PCR reactions were performed with Expand high Fidelity system (Sigma-Aldrich), Phusion DNA Polymerase (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... by PCR and inserted into bacterial expression plasmid pET23a (Novagen, USA) in order to construct either pET-hGFAT2 with and without HisTag ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was digested and ligated into pET20b vector (Novagen) using AscI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR fragment was cloned into the vector pET-11a (Novagen) using corresponding restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... Amplification was performed using REDExtract N Amp PCR ReadyMix (Sigma #R4775) according to the manufacturer protocol.
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bioRxiv - Genomics 2022Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were done using the JumpStart Taq DNA polymerase (Sigma-Aldrich). Primer pairs were used to amplify by PCR flanking regions of approximately 500 bp downstream and upstream of the target genes using the appropriate genomic DNA as a template ...
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bioRxiv - Molecular Biology 2023Quote: ... We used the Expand Long Enzyme PCR system from Millipore Sigma to make small deletions ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification was performed using KOD Hotstart polymerase kit (EMD Millipore) and primers flanking the gRNA target sites (Table 2) ...
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bioRxiv - Genomics 2023Quote: ... All PCRs were performed using KOD Hot Start DNA polymerase (Novagen) according to (46) ...
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bioRxiv - Cancer Biology 2023Quote: The PCR product was then run on 2% agarose (Sigma, #A4718) gel containing Ethidium Bromide (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR cleanup was then performed using Sephadex columns (Sigma-Aldrich). The purified product was sent to Macrogen Europe for sequencing.
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bioRxiv - Microbiology 2023Quote: ... contamination (Lookout Mycoplasma PCR detection kit; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Genomics 2023Quote: ... PCR was performed using JumpStart Taq DNA Polymerase (Sigma-Aldrich, D9307) with the following PCR conditions ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were performed with KOD Polymerase (Novagen, San Diego, CA), and products were cleaned using the ZymoResearch Clean and Concentrator kit (Irvine ...
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bioRxiv - Microbiology 2024Quote: ... We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma) to append a T7 promoter sequence ...
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bioRxiv - Microbiology 2023Quote: ... then purified using GenElute PCR Clean-Up Kit (Sigma, SKU NA1020), and KAPA Pure Beads (Roche-07983298001) ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580, 1:100; anti-ssDNA, Millipore MAB3034, 1:100). After washes with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... then blocked with 5% normal goat serum in 0.5% PBS-T for 2 hr at RT and stained with pre-absorbed rabbit anti-PER (1:1000) 50 and mouse anti-GFP (Sigma G6519, 1:200) in 5% goat serum in 0.5% PBST for at least 48 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently alkylated with 40 mM iodoacetamide in 100 mM Tris (30 min at RT in the dark) (Sigma Aldrich, St. Louis, MO). Samples were diluted 10-fold with 100 mM Tris (pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... gels were incubated for 1.5 hours at RT on a rocking platform with either 20 μg/mL NHS ester-ATTO594 (Sigma-Aldrich, catalog no. 08741) or 20 μg/mL NHS ester-ATTO532 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The blots were washed again in TBST three times for 5 mins each at RT and then exposed to Immobilon Crescendo HRP (Millipore Sigma, Cat # WBLUR0100) for 3 mins at RT and imaged in a Bio-Rad ChemiDoc XRS+ with ImageLab software.
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bioRxiv - Cell Biology 2020Quote: ... in 1× PBS for 30 minutes at RT and subsequently incubated for 1.5 hours in an inert environment with 20 μg/mL maleimide-ATTO594 (Sigma-Aldrich, catalog no. 08717) dissolved in deoxygenated 150 mM Tris-Cl pH 7.4 solution ...
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bioRxiv - Microbiology 2021Quote: ... Organoid sections were air dried and washed three times for 10 minutes at RT followed by blocking and permeabilizing for 60 mins with a solution containing 3% bovine serum albumin (Sigma, Cat. A9418-50G) and 0.1% Triton X-100 in 1XPBS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed for 1 h at RT by addition of 26.6 μL 16% PFA and 4 μg/ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS using standard bore tubing cartridges ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by SYBR® Green RT-qPCR reaction using KiCqStart™ SYBR® Green qPCR ReadyMix™ (Millipore Sigma, Cat # KCQS00). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum containing 0,3% Triton in PBS for 1 h at RT followed by incubation for two overnights at 4°C with mouse anti-acetylated α-tubulin (1:500, Sigma-Aldrich, T7451) and rabbit anti-βIII-tubulin (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... At 24h, cell cultures were fixed with AntigenFix (DiaPath, via Solmedia) for 15min at RT or 3.6% formaldehyde (Sigma-Aldrich; diluted in PBS), and permeabilized with 0.2% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... granulocytes were purified by density centrifugation for 25 min at RT at 800 x g through a Histopaque 1199 gradient (Sigma Aldrich, Taufkirchen, Germany). A discontinuous Percoll® gradient (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and blocked for 1 h at RT with a serum containing 3% bovine serum albumin (BSA; Sigma−Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...