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Citations for Millipore Sigma :
1101 - 1130 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... chloroform extraction and the resulting mixture were dried down under nitrogen and derivatised with 100 µL of 3 M butanolic-HCl (Sigma-Aldrich, Louis, Missouri, USA). Samples were evaporated under nitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... 50 mM Tris–HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Triton X-100, 1×Proteinase inhibitor cocktail SIGMA P9599 and 1 mM EDTA) and incubate the extract at 4 °C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... A final pH of 7.4-7.6 was achieved by adjusting the ratio of Trizma® and Tris-HCl (Sigma-Aldrich, St. Louis, MO, US). Gelatin was not added in this SM buffer.
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bioRxiv - Microbiology 2021Quote: ... epidermidis cells grown overnight in 5 ml TSB were collected and then suspended in 0.5 ml of CS buffer (100 mM Tris-HCl [pH 7.5], 150 mM NaCl, 10 mM EDTA) containing lysostaphin (Sigma-Aldrich, St. Louis, MO, USA) (final concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ml of wash buffer (Tris-HCl pH 7.4, 10mM; NaCl 10mM; MgCl2 3mM; BSA 1%; Tween-20 0.1%; DTT 1 mM; Sigma Protector RNase inhibitor 1 U/μL) was added ...
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bioRxiv - Cell Biology 2022Quote: ... The EV pellets were resuspended in 1 ml of 20 mM Tris-HCl pH 7.5 and mixed with 2 ml of 60% Optiprep™ (Sigma-Aldrich, St. Louis, MO) to create a 40% layer ...
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bioRxiv - Biochemistry 2019Quote: ... Cell pellets were resuspended in PBS and mixed with the equal volume of lysis buffer (3 M NaCl, 100 mM Tris-HCl pH 7.5, 4 mM EDTA pH 8, 20 mM ß-mercaptoethanol, Sigma Protease Inhibitor Cocktail (Sigma)) ...
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bioRxiv - Cell Biology 2020Quote: ... were mixed with a 5-fold volume of lysis buffer (30 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Brij97 (P6136, Sigma-Aldrich, St. Louis, MO, USA)) and protease inhibitor cocktail (25955-11 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in lysis buffer100 (1% SDC, 100 mM Tris-HCl pH 8.5, and protease inhibitors; Millipore Sigma, Cat#: D6750; Cat#: 9290-OP) and boiled for 15 min at 60°C ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated for 1 min with a 1:1 mix of solution A (2.5 mM luminol [Sigma], 0.4 mM p-coumaric acid [Sigma], 100 mM Tris-HCl, pH 8.5) and solution B (0.018 % H2O2 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane pellets were solubilized in 4 mL TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH = 7.4) with 2% Triton X-100 (Sigma-Aldrich, St Louis, MO, USA). The solubilized membrane-fraction was loaded on a column containing 1 mL anti-DYKDDDDK (Flag ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we added an equal volume of stop solution (8 M urea, 1 M Tris–HCl [pH 8.0], and a small amount of Blue Dextran [Sigma Chemical, St. Louis, MO, USA]) to stop the reactions ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were obtained from cell pellets after lysis with 100 μL to 200 μL of sodium-dodecyl-sulfate (SDS) lysis buffer (2% SDS, 10% glycerol, 50 mM of Tris HCL) plus protease inhibitor cocktail (Sigma Aldrich, Catalog. No. 11836170001). Cell lysates were centrifuged for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
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bioRxiv - Cancer Biology 2023Quote: ... we mounted the slides in an anti-bleach imaging buffer containing 2X SSC/10 mM Tris-HCl/10 mM Trolox (Sigma Aldrich, cat. no. 238813)/37 ng/μL glucose oxidase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... were subsequently washed three times in kinase buffer (25 mM Tris-HCl pH 7.4, 100 mM NaCl, 10 mM MgCl2, 50 μg/ml bovine serum albumin [Sigma], 0/1mM EGTA, 0.1% β-mercaptoethanol). Beads (10 μl ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% ascorbic acid was added to a sterile saline solution (NaCl, 0.9% w/v) and 6-OHDA HCl powder (6-hydroxydopamine hydrochloride from Sigma or 6-OHDA hydrobromide from Tocris) was then dissolved to produce a final 6-OHDA concentration of 4.44 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... Precipitated cells were resuspended in 200 µL of TE-buffer (10 mM Tris HCl, 1 mM EDTA, pH 8) containing lysozyme from chicken egg white (Sigma Aldrich, St. Louis, Missouri, USA) at a concentration of 15 mg mL-1 and incubated for 30 min at ambient temperature with regular mixing ...
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bioRxiv - Microbiology 2020Quote: ... They were then resuspended in 250 µL of freshly prepared lysis solution (CelLytic B Cell Lysis Reagent 1x – Sigma -, Tris-HCl pH 7.5 (50 mM), PMSF (0.5 mM) ...
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated for 1 min with a 1:1 mix of solution A (2.5 mM luminol [Sigma], 0.4 mM ρ-coumaric acid [Sigma], 100 mM Tris-HCl, pH 8.5) and solution B (0.018% H2O2 ...
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bioRxiv - Microbiology 2019Quote: ... vulgatus were grown overnight in 5 mL minimal medium supplemented with a final concentration of 10 μM thiamine HCl (THI, > 99% pure) (Sigma Aldrich, St. Louis, MO, USA). Aliquots of each culture were pelleted by centrifugation (1 min at 13,300 x g) ...
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bioRxiv - Plant Biology 2022Quote: ... homogenized in extraction buffer A (10mM Tris-HCl, 0.4M sucrose, 10mM MgCl2, 2mM PMSF, 5mM β-mercaptoethanol, 1µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3), incubated with gentle shaking for 20min ...
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bioRxiv - Plant Biology 2022Quote: ... Pellets were resuspended in 620µl of nuclei lysis buffer (50mM Tris-HCl, 10mM EDTA, 1% SDS, 2mM PMSF, 2µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3). The suspensions were sonicated for 20min with the Bioruptor® Sonication System (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were diluted to a final concentration of 2 mg/mL with Laemelli sample buffer (188 mM Tris-HCl, 218 mM SDS, 8% glycerol, and 500 μM Bromophenol Blue (B5525, Sigma-Aldrich, St. Louis, MO, USA). An equal amount of protein ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then gently washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) and incubated in Alexa Fluor 680 Donkey anti-goat secondary (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times in 1X PBS before lysis in resuspension buffer (50 mM Tris-HCl pH 7.3, 150 mM NaCl, 1:50 diluted Protease Inhibitor Cocktail [Sigma-Aldrich, cat log # P8849], 1% SDS). An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... after 72 h of incubation culture was lysed by freeze-thaw followed by addition of 100 µL of lysis buffer (20□M Tris/HCl (Sigma-Aldrich, St. Louis, MO, United States), 5□M EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM NaF, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 1 mM PMSF, 75 mU/ml aprotinin-Sigma-, 50 mM Tris-HCl, pH 7.5) containing 1% TX-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg RNA was incubated with washed beads (washing buffer: 300 mM NaCl [#AM9760G; Thermo Fisher Scientific], 20 mM Tris-HCl [#T2694; Sigma Aldrich], 0.01% NP-40 [#A2239; AppliChem]) in binding buffer (300 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... samples were collected and incubated in 500 μL of proteinase K solution (100 µg/mL proteinase K [Sigma-Aldrich] in 50 mM Tris-HCl pH 8.0, 2 mM CaCl2) at 55 °C at 800 rpm overnight ...