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Citations for Millipore Sigma :
851 - 900 of 1233 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended in KCl lysis buffer (50 mM Tris-HCl pH 7.5, 225 mM KCl, 1% NP-40) with a protease inhibitor cocktail (Sigma-Aldrich) and other inhibitors (1 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... five fly heads were homogenised in 50 μl GnHCl extraction buffer (5 M Guanidinium HCl, 50 mM HEPES pH 7.3, protease inhibitor cocktail (Sigma, P8340) and 5 mM EDTA) ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) supplemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in non-denaturing lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... to restore osmolarity before being resuspended in 10 mL collagenase buffer (100 mM Tris-HCl, pH=7.4; 1 mM CaCl2) with 1U/mL collagenase (Sigma C0773) and incubated for 2 h at 20°C with gentle agitation ...
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bioRxiv - Molecular Biology 2021Quote: Pellets of cells expressing SFB- or BioID2-tagged viral genes were subjected to lysis with NETN buffer (100mM NaCl, 1mM EDTA, 20mM Tris-HCl, and 0.5% Nonidet P-40) with protease inhibitor cocktail (Sigma-Aldrich) at 4 °C for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... uninfected and SARS-CoV-2-infected Calu-3 cells were lysed in lysis buffer (10 mM Tris/HCl pH 7.4, 150 mM NaCl, 1% Igepal CA630 (Sigma-Aldrich) and 1x cOmplete™ Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Genomics 2020Quote: ... cells were cross-linked with 1% fresh formaldehyde for 15 minutes and treated with lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% IGEPAL CA-630 (Sigma-Aldrich), 1X complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell pellet was resuspended in 1 mL of strep-Tactin lysis buffer (50 mM Tris HCl pH 8.0, 150 mM NaCl) containing protease inhibitors cocktail (1x concentration, cOmplete ULTRA, Sigma-Aldrich) and RNase A (0.1 mg/ml ...
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bioRxiv - Plant Biology 2019Quote: ... in protein sample buffer (50 mM Tris HCl [pH 7.5], 10% glycerol, 2% SDS, 2 mM EDTA, 1 mM DTT and 1% protease inhibitor [Sigma-Aldrich]). Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... pellets were resuspended in 50 μL of lysis buffer (20 mM Tris-HCl pH 7.5, 1 mM EDTA, 0.5 mM Na3VO4, 1 mM benzamidine hydrochloride (Sigma-Aldrich, Germany), 5x cOmplete™ EDTA-free protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The cell pellet was washed with cold acetone and dissolved in 200 µL 6M guanidine HCl (Sigma Aldrich, United States). The absorbance of the solution was measured at 375 nm in Hidex sense microplate reader (Hidex ...
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bioRxiv - Cell Biology 2019Quote: ... washed in 1xPBS before lyzed in resuspension buffer (50mM Tris-HCl, pH 7.8, 150 mM NaCl, 1% SDS and 1x protease inhibitor cocktail (Sigma Aldrich)) and incubated at 95°C for 10 min ...
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bioRxiv - Biochemistry 2019Quote: ... and eluted with 4 x 1 ml elution buffer (50 mM Tris-HCl pH 8.5, 500 mM NaCl, 20 mM reduced L-glutathione (Sigma G4251) and 0.1% TX-100 ...
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bioRxiv - Biophysics 2021Quote: ... was also placed into 7 M Guanidium HCl using three successive concentrations and dilutions using a centrifugal concentrator (3 K MWCO, Amicon Ultra-4, Millipore), and diluted to 2 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: Samples were prepared by combining the indicated concentrations of purified recombinant G3BP1 and NaCl in a buffer containing 50 mM Tris-HCl pH 8.0 and 100 mg/mL Ficoll 400 (Sigma-Aldrich). Samples were sandwiched between a hydrophobic coverslip and microscope slide using an adhesive imaging spacer prior to imaging on a Lecia SP8 Widefield microscope.
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bioRxiv - Biochemistry 2021Quote: ... were mixed with 3 µl proteinase K (20 mg/ml in 25 mM Tris-HCl, 5 mM Ca2Cl, pH 8.0, Sigma-Aldrich). This mixture was applied to the ear clips ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in Triton buffer (50 mM Tris-HCl pH 7.4, 1% Triton-X 100, 137 mM NaCl, 1% Glycerine; Sigma-Aldrich) supplemented with phosphatase and protease inhibitors (cOmplete™ Protease Inhibitor Cocktail and PhosSTOP™ ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed twice with TEN buffer (40 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 7.4) and incubated with Accutase solution (Sigma-Aldrich) at 37 °C for 30 min.
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bioRxiv - Biochemistry 2020Quote: Twenty milligrams of lyophilized biofilm was resuspended in 1 mL of either i) RNase buffer (50 mM Tris HCl, 10 mM EDTA, pH8) with 0.2 mg RNaseA from bovine pancreas (Sigma Aldrich), ii ...
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bioRxiv - Biochemistry 2020Quote: ... ii) storage buffer (10 mM NaCl, 10 mM Tris-HCl) with 0.1 mg Pronase E from Streptomyces grisens (Sigma Aldrich) with 0.5% (v/v) ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell lysates were diluted 10-fold in Tris-HCl buffer and incubated with anti-FLAG M2 agarose beads (Sigma) for 4 h at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Frozen tissue or cell pellets were boiled for 24 hours at 100°C in 500µL (cell pellets) −1mL (tissue) 6M HCl for amino acid analysis (Sigma 84429). 50µL (tissue ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 225 mM KCl, 1% NP-40) with a protease inhibitor cocktail (Sigma-Aldrich) and other inhibitors (1 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were eluted with a 40mM imidazole buffer and buffer exchanged with 20 mM Tris-HCl buffer at pH 8.0 using 10 kDa cutoff Amicon ultrafilter (Millipore, USA). The final concentration (C ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK293T cell lysates transiently expressing mScarlet-I-tagged spartin truncation mutants were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
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bioRxiv - Microbiology 2021Quote: ... membranes were stripped of previously used antibodies using a pH 2.2 glycine-HCl-SDS buffer and reprobed for tubulin (1:5,000, Sigma−Aldrich 6074).
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bioRxiv - Microbiology 2021Quote: ... Brain tissues were surgically removed and minced into small pieces (approximately 1 mm3) on ice and digested by 200 U/ml Papine (Diamond) and Cysteine-HCL (Sigma). Then samples were sieved through 70 m cell strainers ...
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bioRxiv - Microbiology 2020Quote: ... a solution containing 400 nmol/L URO I in 12.5% TCA was prepared using a 1 mM stock solution dissolved in 1 M HCl (Sigma Aldrich). The concentration of the standard was calculated using absorbance at λ = 405 nm and ε = 505 × 103 L cm−1 mol−1 [36] ...
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bioRxiv - Biophysics 2020Quote: ... Chemicals (NaCl, KCl, MgCl2, Tris-HCl, EDTA, PEG800, NaOH, KOAc, KOH, NaOAc) for buffer preparation were purchased from Sigma-Aldrich. Streptavidin was purchased from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Cell Biology 2021Quote: Cells were scraped into ice-cold RIPA buffer (150 mM NaCl, 50 mM Tris–HCl, pH 7.4, 1 mM EDTA, 1% Triton X-100 (Sigma, T8787), 0.1% SDS (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed in 150 μl of ChIP lysis buffer (50 mM Tris-HCl, pH 7.9, 10 mM EDTA, 1% SDS, 1 mM PMSF, protease inhibitor cocktail (Sigma, P8340)) on ice for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were exchanged into Tris buffer (20 mM Tris-HCl, 25 mM NaCl; pH 7.5) by using centrifugal concentrators (30 kDa, Millipore, UFC901096) and concentrated to approximately 6 mg/ml for storage (193 K).
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bioRxiv - Physiology 2022Quote: ... were mixed with 3 µl proteinase K (20 mg/ml in 25 mM Tris-HCl, 5 mM Ca2Cl, pH 8.0, Sigma-Aldrich). This mixture was applied to the ear clips ...
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bioRxiv - Molecular Biology 2022Quote: ... 16806-25] in 10 mM Tris-HCl [pH 8.0], 2× saline sodium citrate [SSC], glucose oxidase [37 µg/mL, Sigma-Aldrich, G2133-10KU] ...
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bioRxiv - Genomics 2022Quote: ... the mice were anesthetized again with 3% isoflurane and immediately administered 100 μl of 15 mM AkaLumine-HCL (Sigma Aldrich) for imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... in a hypotonic buffer (155 mM ammonium chloride, 0.01 M Tris-HCl; Red Blood Cell Lysing Buffer Hybri-Max; Sigma, R7757). Dissociated single cells were then spun at 1,500 RPM for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of each complex was mixed with 7 μl Orange G dye (65% w/v sucrose, 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.3% Orange G powder from Sigma Life Sciences), loaded onto a 1.0% agarose gel ...
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bioRxiv - Plant Biology 2022Quote: ... the tissue was ground to a fine powder with liquid nitrogen and then resuspended in a lysis buffer (50 mM Tris-HCl, pH 7.5; 0.1% [v/v] IGEPAL CA-630 [Sigma-Aldrich]; 2 mM phenylmethylsulfonyl fluoride [PMSF; Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2022Quote: ... then scraped into 3 ml ice cold lysis buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40 substitute (Sigma 74385), 1x Protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... or 500 µl (p35) lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl and 1% Digitonin (Sigma-Aldrich) or 0.5% NP-40 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in 2% CHAPS lysis buffer (20 mmol/l Tris-HCl, pH 7.5; 150 mmol/l NaCl; 1 mmol/l EDTA; 2% CHAPS; Sigma-Aldrich) with protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... of ice-cold sucrose buffer (10 mM Tris-HCl pH 7.4, 0.32 M sucrose) supplemented with a protease inhibitor cocktail (Sigma, 1/100) and homogenized at 4°C using a Teflon-glass potter ...
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bioRxiv - Immunology 2020Quote: ... was dissolved in 150 ml of 1.6 M HCl solution then stirred with 8.6 g of tetraethyl orthosilicate (TEOS, 98%, Sigma-Aldrich, USA) at 40 °C for 20 h ...