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Citations for Millipore Sigma :
1051 - 1100 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... single cell suspensions were centrifuged at 300 rcf for 5 minutes and cell pellets were resuspended in 100 μl of lysis buffer (Tris-HCl pH 7.4, 10mM; NaCl 10mM; MgCl2 3mM; Tween-20 0.1%; Nonidet P40 substitute (Sigma-Aldrich, St. Louis, MO) 0.1% ...
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bioRxiv - Biochemistry 2022Quote: The serum samples were denatured with 6M guanidine chloride (GdmCl) in 100mM Tris/HCl (pH 8.5) and protease inhibitor (Sigma-Aldrich, cat #: P8340), then spun at 21,000xg for 20 minutes at 4 °C to remove insoluble cell contents ...
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bioRxiv - Developmental Biology 2021Quote: ... 60 µl elution buffer (2 M urea, 50 mM Tris-HCl pH 7.5 and 5 μg/mL Trypsin (SIGMA; T6567-5×20UG)) (twice as the bead volume ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in a lysis buffer (20 mMTris-HCl pH=8; 137 mMNaCl; glicerol 10%) supplemented with protease inhibitor (Sigma-Aldrich, USA), and then centrifuged at 10.000g for 15min to the subsequent supernatant storage in −80°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 160 µl of plasma samples were spiked and thoroughly mixed with 40 µl of the internal standard solution containing 1.25 µg/ml diphenhydramine HCl (purity ≥ 99% by HPLC, Sigma-Aldrich, MO, USA). Following the addition of 1.4 ml of trichloromethane ...
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bioRxiv - Biophysics 2019Quote: ... Assembly was initiated by the addition of a salt buffer with a final Tris-HCl concentration of 25 mM and varying amounts of CaCl2 (Sigma, Burlington, MA) and/or ZnCl2 (Sigma ...
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bioRxiv - Genomics 2021Quote: ... then the cells were lysed with Hi-C lysis buffer (10mM Tris-HCl pH8.0 10mM NaCl, 0.2% NP-40) with 1X of protease inhibitors (Sigma complete protease inhibitor cocktail) and the nuclei were recovered ...
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bioRxiv - Physiology 2021Quote: Whole liver tissues were homogenized in lysis buffer containing 20 mM Tris-HCl, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton, phosphatase (Pierce, A32957) and protease inhibitors (Sigma-Aldrich, P8340). Protein concentration was measured with BCA method (Thermo-Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was incubated overnight in antibody buffer (50mM tris-HCl, 150mM sodium chloride, 0.1% tween-20, 2.5% dry milk, all chemicals were purchased from Sigma Aldrich (St. Louis, MO)) with Cu/Zn SOD polyclonal antibody (1:1000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in an EDTA-free lysis buffer containing 20 mM Tris-HCl pH=7.5 and supplemented with a protease inhibitor cocktail (Sigma-Aldrich, Cat. # 4693159001). Calpain activity was measured using the Calpain-Glo assay (Promega ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Genomics 2022Quote: Samples were then co-stained with 5ng/ml DAPI for 15 mins and imaged in an anti-bleaching buffer (50mM Tris-HCl, pH=8.0, 300mM NaCl, 2×SSC, 3mM Trolox (Sigma-Aldrich, Cat. 238813), 0.8% D-glucose (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Thawed INPUTS and eluted IPs were next reverse cross-linked at 65°C o/n after the addition of 16 ul freshly prepared Reverse Crosslinking Salt Mixture (Final 250 mM Tris-HCl, pH 6,5 (Sigma Aldrich, 20-160); 62,5 mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... The purified and cleaved proteins were buffer exchanged into 10 mM Tris-HCl (pH 8.0) and concentrated to 11 mg/ml using an Amicon centrifugal filter unit (Millipore, Burlington, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Imaging acquisition was performed in fresh imaging buffer (50 mM Tris-HCl, pH 8.0, 10 mM NaCl, 10% (w/v) glucose (Sigma-Aldrich, cat#G8270), 1.5 mg MEA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested by centrifugation at 4000 x g and 4°C for 30 min and the pellet was resuspended in 2 mL 50 mM Tris HCl (Carl Roth, #AE15.2) containing 25 mM NaCl (Sigma-Aldrich, #S3014-500G) at pH 8.5 ...
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bioRxiv - Genomics 2023Quote: ... per 632.5 uL 2xTD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% v/v Dimethyl Formamide (Sigma-Aldrich, 227056-100ML)) was added and mixed by pipetting 10 x ...
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bioRxiv - Genomics 2023Quote: ... 160 μl of 2x lysis buffer (2 % Triton x-100, 100 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% sodium deoxycholate (Sigma-Aldrich, 302-95-4), 10 mM sodium butyrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... dried phosphopeptides from each fraction were re-dissolved in 800 μL cold IAP buffer (50 mM Tris-HCl, 150 mM NaCl, 1% w/v n-Octyl-β-D-glucopyranoside (EMD Millipore), EDTA-free Halt protease inhibitor and phosphatase inhibitor cocktails ...
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bioRxiv - Biochemistry 2023Quote: ... Disulfide formation was determined by injecting protein at 1.5 mg/mL in the presence and absence of 50 mM TCEP-HCl (Millipore Sigma 646547-10X1ML) and detecting the mass shift.
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... and each piece soaked in 0.5 ml SDS lysis buffer (50 mM Tris/HCL [pH 8.0], 1% SDS, 5 mM EDTA) containing Roche protease inhibitors (Sigma-Aldrich, Catalog # 11836170001). The soaked tissue was first sonicated for 8 sec for three times at 30% amplitude with a Bronson sonicator equipped with a microtip ...
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bioRxiv - Neuroscience 2023Quote: ... A subset of cells was rinsed twice with phosphate buffered saline and incubated with lysis buffer (1M Tris HCl [pH 7.5], NaCl, NP-40, and protease inhibitor cocktail [Sigma Aldrich, Catalog #P8340]) for 20 min at 4°C on a platform shaker ...
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bioRxiv - Neuroscience 2023Quote: ... per 632.5 uL 2xTD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% v/v Dimethyl Formamide (Sigma-Aldrich, 227056-100ML)) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then gently washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) and incubated in Alexa Fluor 680 Donkey anti-goat secondary (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellets were resuspended in 200 μl of digestion buffer (1 % SDS, 100 mM NaCl, 50 mM Tris-HCl, 10 mM EDTA and 50U Lyticase (Sigma-Aldrich, L4025)) and incubated at 37ºC ...
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bioRxiv - Cancer Biology 2023Quote: NSG mice bearing subcutaneous transplants of Hopx-MACD reporter cells were dosed with 100 µl of 30 mM of AkaLumine-HCl substrate resuspended in PBS (#808350, Millipore Sigma, TokeOni) and imaged on an IVIS Lumina II (Perkin Elmer)(23).
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted on glass microscope slides using mounting solution (0.1M Tris-HCl, pH 8.5, 10% Mowiol [Sigma, UK] and 50% glycerol) containing 2.5% DABCO (1,4 diazabicyclo(2.2.2)octane ...
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bioRxiv - Cell Biology 2023Quote: ... buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich], 1% sodium deoxycholate [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2023Quote: ... then lysed in 1ml lysis buffer (50mM Tris-HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Trion-X100 with complete protease inhibitors (Sigma 11836170001, added fresh) and PhosSTOP (Sigma 4906845001) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... the pelleted cells were resuspended in TE buffer (30 mM Tris-HCl, 1 mM EDTA, pH = 8.0) supplemented with lysozyme 20 mg/mL (Sigma-Aldrich, Saint Louis, Missouri) and 80 μg/mL lysostaphin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... were synthesized using a chemical reduction method (Frens et al.)58 by heating up 10 µL HAuCl4 (99.99% trace metals basis, 30 wt. % in dilute HCl, Sigma-Aldrich, St. Louis, MO) in 29.99 mL ultrapure water (0.5 mM ...
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bioRxiv - Systems Biology 2021Quote: ... and kept at -20 °C. Chlorpromazine (in house synthesized based on (Galons et al. 1985)) and loperamide HCl (Sigma-Aldrich, Merck KGaA, Germany) were freshly dissolved in water and filtered prior to the treatment ...
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bioRxiv - Biochemistry 2021Quote: ... color reactions were developed with p-nitrophenyl phosphate (1 mg/ml in Tris-HCl with 1 mM MgCl2) (Sigma-Aldrich, St. Louis, MO). Plates were read using 2300 EnSpire Multilabel Reader (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... at 37°C and hemisections obtained from the same slice were treated with either vehicle or 1 μM (-)-Quinpirole HCl (Sigma, St. Louis, MO) for 5 min (biotinylation ...
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bioRxiv - Cell Biology 2021Quote: ... 2.7 mM KCl [Sigma-Aldrich P9541], 19 mM Tris-HCl pH 7.5, and 0.1% v/v Tween 20 [Sigma-Aldrich P7949] in water) for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... were first washed twice and resuspended in nuclei lysis buffer (50 mM Tris-HCl pH = 8.0, 10 mM EDTA, 1% SDS) supplemented with cOmplete Protease Inhibitor Cocktail (Sigma, St Louis, MO, USA) and RNaseOUT (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Protein extraction from the remaining EDTA-insoluble pellet was performed with a 4M guanidine hydrochloride (Gu-HCl, Sigma-Aldrich St. Louis, MO, USA) solution at pH 7.4 containing protease inhibitors ...
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bioRxiv - Physiology 2021Quote: ... and tracrRNA were resuspended to 100 μM in nuclease-free and sterile T10E0.1 buffer (10 mM Tris-HCl, 0.1 mM EDTA, embryo-tested water, Sigma, W1503, St. Louis, Missouri, USA) prepared as described (51) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10% glycerol, 0.5% IGEPAL, 100 μg/ml RNaseA, 1 tablet of Sigma-Aldrich cOmplete Protease Inhibitor Cocktail). Resuspensions were vortexed ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Neuroscience 2023Quote: ... The dried polar metabolite pellet was derivatized by a two-step methoxyamine protocol first by addition of 50 μL methoxyamine HCl (Sigma-Aldrich #226904-5G) in pyridine (20 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... After a few washes in TBST, staining was performed in NTM (2M Tris-HCl, 2M NaCl, 2M MgCl2) and NBT-BCIP (Sigma, final concentration 0.3%), for 24 h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... all His-SIRT5 were equalized to 320 µg/ml with exchange buffer (25 mM Tris/HCl pH 8.0, 100 mM NaCl) containing 20x Sypro Orange (#S5692, Sigma-Aldrich, delivered at 5000x). Samples (25 µl ...
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bioRxiv - Neuroscience 2023Quote: ... Non-specific sites were blocked by incubation with 5% BSA in Tris-buffered saline with 0.1% Tween® 20 detergent (TBS-T, 20 mM Tris-HCl and 150 mM NaCl, Sigma-Aldrich, Cat. Nos. T5941, T1503, S3014, with Tween® 20 detergent, Sigma Aldrich, Cat ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µM ecTrxB or bsTrxB were added to TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5) containing 200 µM NADPH (Sigma-Aldrich, St. Louis, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Chilled lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, and 0.1% Nonidet™ P40 Substitute (Sigma Aldrich, PN-74385) was added to the tissue and the tube was incubated on ice for 15 min with gentle shaking during the incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Cat # 885300-0001) filled with 300 μL of ice cold NP40 lysis buffer (10 mM Tris-HCl (Sigma-Aldrich, Cat. # T2194; pH 7.4), 10 mM NaCl (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... in 100 µL TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5) containing 200 µM NADPH (Sigma-Aldrich, St. Louis, USA). Protein concentration was determined through absorption at 280 nm using the molar extinction coefficients 18910 M-1cm-1 (ecTrxB) ...
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bioRxiv - Microbiology 2019Quote: ... and lysed with NP-40 lysis buffer (1% NP-40 in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) containing protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Lysates and supernatants were stored at -80°C until use.