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Citations for Millipore Sigma :
651 - 700 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Ovaries were disrupted using RNase-free 1.5mL pestles (SLS) in lysis buffer (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... twice in Tris-HCl (pH 8.5) and three times in 100 mM triethylammonium bicarbonate buffer (TEAB) pH 7.5 (Sigma-Aldrich). The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed using a urea-thiourea buffer (7M urea, 2M Thiourea, 4% CHAPS, 0.1% β-mercaptoethanol and 30mM Tris HCl pH8.5, all from Sigma) supplemented with cOmplete™ Protease Inhibitor Cocktail (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were washed with ice-cold PBS and resuspended in Homogenization buffer (10 mM Tris-HCl pH 6.7, 10 mM KCl, 0.15 mM MgCl2, 1 mM PMSF (Sigma, 10837091001)) ...
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bioRxiv - Bioengineering 2022Quote: AuMA NPs were synthesized by covalently-linking AuCOOH NPs with 2-aminoethyl methacrylate (AEMA, 90%, C6H11NO2·HCl, Sigma-Aldrich) using carbodiimide/succinimide chemistry (Fig ...
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bioRxiv - Biophysics 2022Quote: ... permeabilized with 0.2% Triton X-100 in TBS (50 mM Tris-HCl, 0.15 M NaCl, pH 7.4) and blocked with 2% BSA (Sigma Aldrich) in TBS ...
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bioRxiv - Plant Biology 2022Quote: ... the tissue was ground to a fine powder with liquid nitrogen and then resuspended in a lysis buffer (50 mM Tris-HCl, pH 7.5; 0.1% [v/v] IGEPAL CA-630 [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were thereafter washed three times with 1 ml wash buffer (50 mM Tris/HCl, pH 7.5, 150 mM NaCl and 0.5 % Digitonin (Sigma-Aldrich) or 0.5% NP-40 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Ovaries and testes were disrupted using RNase-free 1.5 mL pestles (SLS) in lysis buffer A (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were prepared for one colour 2D d-STORM utilizing a buffer containing 100 mM MEA-HCL (Sigma, M6500), 10% glucose (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... The 50 µl pre-washed with washing buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0,1% Tween-20) M2 anti-FLAG magnetic beads (Sigma) was added to supernatant ...
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bioRxiv - Microbiology 2021Quote: ... were harvested by centrifugation and suspended with lysis buffer containing 50 mM Tris HCl pH 8.0 with 10 μg/mL DNase I (Sigma), 50 μg/mL lysozyme (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: HEK 293T cells or DRG sensory neurons were collected and prepped with lysis buffer (1% NP-40; 50mM Tris-HCl, pH 7.4; 150mM NaCl; 2mM EDTA; protease inhibitor (Sigma); and phosphatase inhibitor (Life Technologies)) ...
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bioRxiv - Cell Biology 2020Quote: ... Incubation media were collected and 200 μl were mixed with 800 μl Tris-HCl [pH 7.5] 0.02M/activated charcoal slurry (Sigma-Aldrich) 10% for 1 hr on a rotational shaker90 ...
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bioRxiv - Microbiology 2021Quote: ... we replaced the lysis solution sequentially with ESP (10 mM Tris HCl pH 7.6, 1 mM EDTA) to which we added proteinase K (Sigma) at a final concentration of 100 µg/ml and 1% sodium dodecyl sulfate at 50°C for one hour ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed with TNE buffer (Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA) supplemented with 1% triton X-100 (Sigma), protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... and buffer exchanged into 50 mM trisaminomethane-hydrochloride (Tris-HCl) at pH 7.4 using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting cell pellet was re-suspended in cold (4°C) 0.2 Normal hydrochloric acid (HCl; Sigma-Aldrich, 343102) at a density of ∼ 1 x 107 cells/mL and incubated at 4°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were harvested at 1,000 g for 10 min and subsequently resuspended either in 1 ml ice cold IPP150 buffer (10 mM Tris-HCl pH 6.8, 150 mM NaCl, 0.1% IGEPAL CA-630; Sigma I8896) or in CHAPS buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... 50,000 of cells for each experimental replicate and/or condition were resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma aldrich) to perform crude nuclei isolation ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed with 0.5N HCl and stained overnight with 0.25% (w/v) Alcian Blue 8GX (Sigma-Aldrich) in 0.5N HCl ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed in RIPA buffer (30 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Igepal (Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Genomics 2019Quote: ... were treated with ice-cold lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% TX-100, 0.5% IGEPAL-CA-630, Sigma; 5mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... lysate was layered on a sucrose gradient (10-50%) in PLB buffer (20mM Tris-HCl, pH 7.4, 150mM NaCl, 10mM MgCl2, 100µg/ml cycloheximide (Sigma-Aldrich), 0.5mM DTT ...
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bioRxiv - Genomics 2019Quote: ... samples were resuspended in 500 µL of ice-cold cell lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.5% Igepal CA-630, supplemented with the protease inhibitor cocktail from Sigma-Aldrich), incubated for 20 min on ice ...
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bioRxiv - Biophysics 2019Quote: ... was diluted 1:100 to 8 units/mL in digestion buffer (50 mM Tris/HCl pH 8, 1 mM EDTA, 0.5% [v/v] Triton X-100, 1 M NaCl, Sigma) and incubated with the gels fully immersed in proteinase solution overnight at 23 °C ...
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bioRxiv - Genomics 2019Quote: ... the cells were pelleted and resuspended in 1 ml of lysis buffer B [10 mM Tris-HCl (Sigma, T2663) pH 8.0 ...
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bioRxiv - Pathology 2019Quote: ... 20 mM Tris-HCl [pH 7.4], 1 mM EDTA, 5 mM DTT, and 50 µL protease inhibitor cocktail [Sigma]) and homogenized on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in Buffer A (25 mM Tris-HCl pH 8.5, 150 mM KCl, 50 mM Imidazole, 10% glycerol) (Sigma) with 25 mM Magnesium chloride ...
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bioRxiv - Bioengineering 2020Quote: ... were dissolved in 150 g of 1.6M HCl solution and stirred with 8.6 g of tetraethyl orthosilicate (TEOS, 98%, Sigma-Aldrich) at 40 °C for 20 h ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with cold PBS and incubated for 15 minutes at 4°C with RIPA lysis buffer (30 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10% glycerol, 1% Triton X-100 (Sigma) supplemented with a 1 mM NaF ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were solubilized in HU buffer (8 M urea, 5% SDS, 200 mM Tris-HCl pH 6.8, 20 mM dithiothreitol (DTT, Sigma) and bromophenol blue 1.5 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 3 times with 1x Binding Buffer and mRNP complexes were eluted in 50 μl Elution Buffer (10 mM Tris-HCl pH7.5, 1mM EDTA) supplemented with Complete protease inhibitor and 10 μg RNase A (Sigma) at 37°C for 30 min with gentle agitation ...
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bioRxiv - Microbiology 2019Quote: ... Water-free ethanol was prepared by adding 2 ml acidulated 2,2-dimethoxypropan (two drops of 37% HCl ad 100 ml 2,2-dimethoxypropan (Sigma Aldrich)) to 100 ml ethanol absolute (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurospheres from each condition were lysed in buffer containing 0.1% triton X-100 in 50 mM Tris-HCl and 150 mM NaCl and Protease Inhibitor Cocktail (Sigma). Samples were then sonicated and subsequently spun down at 13,000 RCF for 10 minutes at 4°C ...
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bioRxiv - Genetics 2021Quote: ... were resuspended in 4 volumes lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM dithiothreitol, 1 mM ethylenediaminetetraacetic acid, 1:400 Sigma protease inhibitory cocktail P8340 ...
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bioRxiv - Immunology 2020Quote: ... each of the anti-MuSK clones was combined with 1.3-1.4x molar excess of the b12 antibody and 75 mM 2-Mercaptoethylamine-HCl (Sigma-Aldrich) for five hours at 31°C (Labrijn et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed with TNE buffer (Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA) supplemented with 1% triton X-100 (Sigma), protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2019Quote: ... freshly purified tau was first reduced with 1 mM DTT for 10 minutes and buffer exchanged into a labeling buffer (20 mM Tris pH 7.4, 50 mM NaCl, and 6 M guanidine HCl) using Amicon concentrators (Millipore) (17) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The cells were re-suspended in 50 ml of TE (10 mM Tris-HCl pH 8.0, 1 mM EDTA) that contained chicken lysozyme (1 mg/ml, Sigma) and DTT (10 mM ...
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bioRxiv - Neuroscience 2019Quote: ... samples were mixed with chilled buffer (50 mM Tris-HCl pH 9, 1% DOC, 50 mM NaF [Merck-Millipore] ...
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bioRxiv - Neuroscience 2019Quote: Protein samples for electrophoresis were prepared with Laemmli loading sample buffer (50 mM Tris-HCl, pH 6.8; 2% SDS [Merck-Millipore] ...
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bioRxiv - Genetics 2020Quote: ... 10mM Tris HCl, pH 7.5 [Fisher, Cat. T1503 and Fisher, Cat. A144], 10mM NaCl [Fisher, Cat. M-11624], 3mM MgCl2 [Sigma, Cat ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM Tris-HCl pH 7.5) and spheroplasts were prepared by addition of 600 U of lyticase (Sigma L4025) and 10 mM DTT during incubation at 30°C with agitation for 45 min ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT), then incubated with cleavage buffer (20 mM Tris-HCl [pH 8.0], 150 mM NaCl) containing thrombin (Sigma) overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol), then incubated with cleavage buffer (20 mM Tris-HCl [pH 8.0], 150 mM NaCl) containing thrombin (Sigma) overnight at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... Purified RBCs were lysed with 5 volumes of hypotonic lysis solution (5 mM Tris-HCl pH 7.4) containing EDTA-free protease inhibitor cocktail tablets (Sigma) and phosphatase inhibitor tablets (PhosSTOP EASY ...