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Citations for Millipore Sigma :
701 - 750 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... 1.0% Triton X-100, 0.1% SDS), twice with LiCl wash buffer (10mM Tris-HCl pH 8.0, 1mM EDTA, 250mM LiCl (Sigma Aldrich), 0.5% NaDeoxycholate ...
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bioRxiv - Cell Biology 2021Quote: ... cells were harvested in IP buffer (0.5% Triton X-100, 50 mM Tris-HCl pH 7.5, 150 mM NaCl, protease inhibitor cocktail (Sigma-Aldrich)) and the lysates were cleared by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma] ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 37 °C and with proteinase K buffer (50 mM Tris-HCl pH 8.0, 5% glycerol, 2.5 mM CaCl2, 1 mg/ml proteinase K (Sigma P2308)) for 30 min at 50 °C ...
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bioRxiv - Microbiology 2020Quote: ... Purified Vag8 was concentrated and the buffer was exchanged to buffer F (50 mM Tris-HCl pH 8.0, 150 mM NaCl) by ultrafiltration (Amicon Ultra, Merck-Millipore).
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bioRxiv - Immunology 2021Quote: ... or a combination of plate-bound anti-CD3 (OKT3, 10 μg/ml in 0.1 M Trizma HCl; Sigma-Aldrich) with soluble anti-CD28 (CD28.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were resuspended in lysis buffer (400 mM KCl, 10 mM Tris-HCl pH 8.0 and protease inhibitors (20 μM leupeptin (Sigma), 2μg ...
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bioRxiv - Molecular Biology 2020Quote: ... The target protein was eluted with 20 ml elution buffer 3 (600 mM NaCl, 20 mM Tris-HCl, pH 7.4, 2.5 mM desthiobiotin (Sigma-Aldrich), and 1 mM DTT).
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bioRxiv - Genomics 2019Quote: ... Each kidney was then transferred to a 1.5mL tube containing 300µL of chilled lysis buffer (10mM Tris-HCl [Invitrogen; AM9856], 3mM MgCl2 [Invitrogen; AM9530G], 10mM NaCl [Sigma; 71386] ...
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bioRxiv - Molecular Biology 2021Quote: ... Pellets were resuspended in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0; Sigma-Aldrich) and reverse-crosslinked ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were lysed in NP-40 lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% (v/v) IGEPAL CA-630 (Sigma-Aldrich), 1 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was suspended in buffer solution (20u Tris-HCl pH 7.5, 2u u mM 2-mercaptoethanol) with protease inhibitor cocktail (Sigma), 10 μg/mL aprotinin ...
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bioRxiv - Microbiology 2022Quote: ... washed twice with ice-cold buffer A (50 mM Tris-HCl, 200 mM KCl, pH 8.0 at 4 °C, filtered through a 0.22-μM Millipore membrane), resuspended in buffer A with 20 mM imidazole added ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in 100 mL lysis buffer (20 mM TRIS-HCl pH 8.0, 500 mM NaCl) supplemented with 50 μg lysozyme (SIGMA), and 50 μg DNase (SIGMA ...
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bioRxiv - Bioengineering 2022Quote: ... Total plant proteins were extracted using native buffer (10mM Tris/HCl pH 7.5, 150mM NaCl, 0.5mM EDTA, 1% [v/v] P9599 Protease Inhibitor Cocktail [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2022Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was then transferred into a new tube and volume was increased to 1 ml /sample with ChIP Dilution Buffer (Final 16,7mM Tris-HCl, pH 8,0; 1,2mM EDTA (Sigma Aldrich, 03690)) ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings were lysed in lysis buffer (1% Triton X-100, 50 mM Tris–HCl pH 8.0) supplemented with 1X plant protease inhibitor cocktail (Sigma) and 25 mM N-ethylmaleimide ...
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bioRxiv - Immunology 2022Quote: ... the feces samples were weighed and then resuspended for 10 min at room temperature in 250 μl of 4 M guanidine thiocyanate in 0.1 M Tris-HCl (pH 7.5) (Sigma-Aldrich) and 40 μl of 10% N-lauroyl sarcosine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Plant Biology 2022Quote: ... The GST protein was eluted from the beads by elution buffer (50 mM Tris-HCl pH 9.0, 10 mM reduced glutathione (Sigma)) and concentrated by Amicon Ultra-4 Centrifugal Filter Unit (Merck).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in lysis buffer (50 mM Tris–HCl pH 8.0, 1% SDS) containing protease inhibitor cocktail (Sigma) and sonicated ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets from a total of ∼5ODs were resuspended in 400μl lysis buffer (150 mM NaCl, 50mM Tris-HCl pH 8.0, 5% Glycerol, 1% digitonin (Sigma, #D141), 1mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and eluted with 5 CV of elution buffer (10 mM reduced glutathione [Sigma], 50 mM Tris-HCl, pH 9.0). Protein purity was determined via SDS–PAGE (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... in 1000 mL milliQ H2O adjusted to pH 6.0 with 1N HCl) + 0.05% TWEEN 20 (Sigma-Aldrich, P1379-1L) in a 85°C water bath for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected in lysis buffer (150mM NaCl, 50mM Tris-HCl pH 8, 1mM DTT, 0.5% NP-40, with protease and phosphatase inhibitors (Sigma).After 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors, 0.1% Triton X 100 Sigma) via dounce homogenization on ice ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in NP40 lysis buffer (50mM Tris HCl pH7.4, 1mM EDTA, 150mM NaCl, protease inhibitors (all purchased from Sigma)) ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... and an extract of total cellular proteins was prepared by harvesting the cells by centrifugation and resuspending cell pellets in 50 mM Tris-HCl (pH 8.0) (200 µl per OD unit) and incubating with 5 µg ml-1 lysostaphin (Sigma) for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The harvested cells were resuspended in immobilised metal chromatography (IMAC) buffer (50 mM Tris-HCl [pH 7.5], 300 mM NaCl) supplemented with 3 kU/ml rLysozyme (Novagen). The cell suspension was sonicated on ice (Ultrasonic disruptor UD-201 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were resuspended in IP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 0.5% IGEPAL® CA-630 (Sigma)) supplemented with Pierce™ Protease Inhibitor Mini Tablet (ThermoFisher A32955 ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Finally the membrane pellet was resuspended in 500 µL of CLIP lysis buffer (50 mM Tris-HCl pH 7.5, 200 mM NaCl (Sigma), 1 mM EDTA ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were lysed with TNE buffer (Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA) supplemented with 1% Triton X-100 (Sigma), protease inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... in 100 µL extraction buffer (10 mM tris-HCl pH8.0, 100 mM NaCl, 50 mM EDTA, 1% SDS, 10% 10 mg/mL proteinase K, Sigma), harvested by scaping ...
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bioRxiv - Neuroscience 2023Quote: ... glass was cleaned in 3 N HCl for 48 h and immersed in sterile aqueous solution of 0.1 mg/mL poly-L-lysine (P4707, Sigma) for 12 h ...
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bioRxiv - Molecular Biology 2023Quote: The cell pellet was resuspended in 900μl of ice-cold polysome lysis buffer (20 mM Tris-HCl (Sigma, T294), pH=7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA fibers were then denatured in 2.5 M HCl solution for 1 hr at room temperature and blocked with 5% bovine serum albumin (BSA; Sigma) in 1xPBS for 45 min at 37°C ...
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bioRxiv - Genetics 2023Quote: ... cells were washed with PBS and harvested using Cell Lysis Buffer (150 mM NaCl, 0.1% Triton X-100, 50mM Tris-HCl pH 8.0, Protease inhibitor (Sigma Aldrich)) ...
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bioRxiv - Cell Biology 2022Quote: ... the tubes containing the pre-cut tissue were thawed and 4mL of cold 1X CST buffer (salts and Tris buffer, including NaCl, Tris-HCl pH 7.5, CaCl2, and MgCl2, with CHAPS hydrate (Sigma), BSA (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were incubated with the samples for 1 h at room temperature with gentle rotation before two washes with Tween wash buffer (5 mM TRIS-HCl, pH 7.5 (Sigma), 0.5 mM EDTA (Applichem ...
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bioRxiv - Cell Biology 2022Quote: ... Imaging buffer (100□mM Tris–HCl at pH□8.0, 20□mM NaCl and 10% glucose; all from Sigma-Aldrich) and an oxygen-scavenger system (60□mg ml−1 glucose oxidase ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellet was resuspended in ChIP lysis buffer (50mM Tris-HCl, pH 8.0; 10mM EDTA; 1%SDS) containing COmplete EDTA free protease inhibitors (Sigma), and quantified with BCA ...
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bioRxiv - Cell Biology 2023Quote: ... once with TEL buffer (10 mM Tris-HCl, pH 8.0, 250 mM LiCl, 1% IGEPAL CA-630 [Sigma-Aldrich, #I3021] ...
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bioRxiv - Molecular Biology 2023Quote: ... sperm pellets were resuspended in NP40 Buffer (10 mM Tris-HCl, pH 8.0, 10 mM NaCl, 0.5% NP40, 1X complete EDTA-free protease inhibitors, Sigma Aldrich) and incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... and gently resuspended in cold fractionation buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 0.5% v/v IGEPAL® CA-630 (Sigma)) supplemented with Pierce™ Protease Inhibitor Mini Tablet (ThermoFisher A32955 ...
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bioRxiv - Biochemistry 2023Quote: ... followed by suspending in IP buffer (50 mM Tris-HCl pH 7.4, 100 mM NaCl, 0.5% IGEPAL® CA-630 (Sigma)) with one Pierce™ Protease Inhibitor Mini Tablet (ThermoFisher A32955) ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were resuspended in GST-Pull Down equilibration/wash buffer (125 mM Tris-HCl pH 7.5, 150 mM NaCl + Protease Inhibitor Cocktail (Sigma) + 1 mg/ml lysozyme ...
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bioRxiv - Microbiology 2023Quote: ... or in 6His-Pull Down equilibration/wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl and 10 mM Imidazole + Protease Inhibitor Cocktail (Sigma) + 1 mg/ml lysozyme ...