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Citations for Millipore Sigma :
1001 - 1050 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... sorted using a BD FACSAriaTM and lysed in 6 M urea/ 2 M thiourea supplemented with 10 mM tris(2-carboxyethyl)phosphine (TCEP) and 40 mM chloroacetamide (CAA) in 75 mM NaCl and 50 mM Tris-HCl (Sigma-Aldrich) and sonicated using a metal tip ...
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bioRxiv - Bioengineering 2023Quote: ... extraction was performed by lysing a maximum of 5 x 106 cells for 18-24hr at 55°C 487.5 µL TENS buffer (10 mM Tris-HCl (Sigma, cat. T3253) pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... 2-8 µM stock of J1 was prepared by mixing the stands at 1:1:1:1 ratio in 20 mM Tris-HCl (Sigma-Aldrich), pH 7.5 with an requied concentration of MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in binding assay buffer (50 mM Tris HCl, 5 mM MgCl2, 1 mM CaCl2, 100 mM NaCl and 0.1% fatty acid free BSA, Sigma, pH 7.4). For the assay ...
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bioRxiv - Pathology 2023Quote: ... Slides were then briefly submerged in acid alcohol (300 ml dH20, 700 ml 100% ethanol, 3 ml concentrated HCL; Sigma-Aldrich), followed by tap water wash ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgSO4, 5 mM 6-aminocaproic acid (Sigma, cat. #A2504), 5 mM benzamidine (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... This solubilized protein was dialyzed overnight against 20 mM Tris–HCl (pH 7.0) and 50 mM NaCl and then loaded onto an anionic diethylaminomethyl –Sephacel (Sigma-Aldrich I6505) equilibrated with the same buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% SDS (10 mM Tris-HCl, pH 8.0; 1% SDS; 1 mM Na-Orthovanadate; 1x Protease Inhibitor Cocktail (Sigma-Aldrich; P8340)) and incubated for 10 min at 95°C ...
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bioRxiv - Cancer Biology 2023Quote: ... prepared in buffer G (20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2 supplemented with 1 mM DTT (Sigma Aldrich) supplemented with fresh 100 μg/ml cycloheximide ...
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bioRxiv - Cell Biology 2023Quote: ... and cells rinsed in 5 ml of 50mM Tris-HCl (pH 7.4) and nuclease buffer containing 10 U/mL Benzonase® Nuclease (Sigma E1014), 10 mM MgCl2 and 2 mM CaCl2 in 50 mM Tris-HCl buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysis was conducted using thawed pellets in lysis buffer (50mM Tris-HCL, 10mM imidazole, 500mM NaCl, pH 8.0) with 1x protease inhibitor cocktail (Sigma-Aldrich: S8830) using probe tip sonication ...
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bioRxiv - Bioengineering 2023Quote: Precultures were grown in mineral media (Figure 3) or buffered SD media supplemented with 2000 µg/L thiamine (thiamine HCL, Sigma-Aldrich) (Figure 5 ...
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bioRxiv - Genomics 2023Quote: ... 1% Triton X-100 Buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1%Triton X-100) supplemented with protease inhibitors (Sigma-Aldrich #11836170001) was used for lysis ...
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bioRxiv - Cell Biology 2023Quote: ... twice with wash buffer 3 (20 mM Tris-HCl pH 7.5, 2 M NaCl and 0.5% IGEPAL CA-630 [Sigma-Aldrich, cat#I8896]), and twice with wash buffer 4 (50 mM Tris-HCl pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... SsoPox variants were used at 1 mg.mL-1 in Tris buffer (50 mM Tris-HCl, pH 7) and with 1X SYPRO® orange (Sigma-Aldrich). Denaturation was followed using FRET channel ...
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bioRxiv - Bioengineering 2023Quote: ... pH 14), hydrogen chloride solution (c(HCl)=1, pH 1), and buffer solution (pH 4, pH 9) were purchased from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB ...
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bioRxiv - Neuroscience 2023Quote: ... Non-specific sites were blocked by incubation with 5% BSA in Tris-buffered saline with 0.1% Tween® 20 detergent (TBS-T, 20 mM Tris-HCl and 150 mM NaCl, Sigma-Aldrich, Cat ...
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bioRxiv - Microbiology 2023Quote: ... oocysts were excysted in vitro by treating them first with 10mM HCL (37C, 10 minutes) and then 2mM sodium taurocholate (16C, 10 minutes; Sigma T4009). Host cells are grown to 90% confluency in appropriate plates for infection ...
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed with high-salt buffer (300nM NaCl, 50mM tris-HCl pH8, 10% glycerol, and 0.2% NP-40) supplemented with protease inhibitors (Sigma-Aldrich, #04693132001) and then were sonicated with a Bioruptor for 5min 30’’ on/off ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Cell Biology 2023Quote: ... from MeOH/TBST (0.15 M NaCl, 0.1 M Tris-HCl and 0.1% Triton® X-100 (Cat. No. 108603; Sigma-Aldrich, Merck, Germany), pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Testes from 4-week-old male mice and tissues from Rag1−/– female mice were washed with PBS and then homogenized in TNE buffer (10 mM Tris-HCl, 150 mM NaCl, 10 mM Triton X-100, and cOmpleteTM (Sigma-Aldrich); pH 7.4) ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were imaged under a microscope after the addition of an anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2xSSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Neuroscience 2023Quote: ... After discarding the PBS, we added a pre-heated dissociation solution (Mix A [40 ml 1X PBS, 10 mg DL-cysteine HCl (Sigma C9768), 10 mg BSA ...
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bioRxiv - Cell Biology 2024Quote: ... mucus from injected animals was removed by cooling worms on ice for 1 min then incubating on ice-cold 2% HCl (Sigma H1758) for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and collected on wet ice in 4°C RIPA lysis buffer (150 mM Tris-HCl, 150 mM NaCl, 0.5% (w/v) sodium deoxycholate (Sigma-Aldrich, D6750), 1% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% - 30% sucrose gradients prepared in buffer G (20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 1 mM DTT (Sigma Aldrich), 100 μg/ml chloramphenicol (Sigma Aldrich)] and ultracentrifuged using an SW41-Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2x 5 min washes with Tris-HCl 0.05M before been placed for 25 min in developing solution (3,3-diaminobenzidine tetrahydrochloride, DAB; Sigma-Aldrich, D-9015) with 0.006% H2O2 ...
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bioRxiv - Cancer Biology 2024Quote: Tissues were homogenized in cold RIPA lysis buffer (150 mM Tris-HCl, 150 mM NaCl, 0.5% (w/v) sodium deoxycholate (Sigma-Aldrich, D6750), 1% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed three times with DPBS for 5 minutes each and then treated with 0.1 M HCl (Sigma-Aldrich, #320331) diluted with DPBS for 5 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... and 4.5 µL aliquots were combined at different times with 0.5 µL of 10X GODCAT visualization buffer (50 mM Tris-HCl pH 7.5; 2 mg/mL Glucose Oxidase (SIGMA cat. no. G2133); 350 ng/mL Catalase (SIGMA cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We added 100 μl of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 nM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630 (Sigma-Aldrich, St. Louis, MO)) and ground the cells with a pestle ...
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bioRxiv - Molecular Biology 2021Quote: For protein purification approximately 0.1g of powdered tissue from each procedure was dissolved in 0.8 ml of phenol (buffered with Tris HCL, pH8.0, Sigma, St. Louis, MO, USA) and 0.8 ml of SDS buffer (30% sucrose ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with 4N HCl at RT for 30 min and incubated with mouse anti-BrdU at 1:100 (B2531, Sigma, Milan, Italy) in blocking solution (0.1% BSA in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... pastoris cells were grown in liquid YPD or solid YPD-agar and selected for with the appropriate antibiotics: 100 µg/mL Zeocin® or 300 µg/mL BlasticidinS-HCl (Sigma Aldrich). Transformants of auxotrophic strains were selected on Complete Supplemented Medium (CSM ...
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bioRxiv - Biophysics 2020Quote: ... Wet chemical etching solution for Au: 20 mM Fe(NO3)3·9 H2O (Fluka, Switzerland), 30 mM thiourea (Fluka, Switzerland) and 1 mM HCl (Sigma-Aldrich, USA) dissolved in ultrapure water saturated with octanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue from three rats per sex was combined and transferred to 5 ml of ice cold lysis buffer (10 mM tris-HCl, 10 mM NaCl, 3 mM MgCl2 and 0.1% Igepal in nuclease-free water (Sigma-Aldrich, 18896-50ML) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates for immunoblot analysis were prepared from cells after functional analysis using NP-40 lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris-HCl pH7.6) supplemented with 1× protease inhibitor cocktail (Sigma-Aldrich, cat #11836170001). Protein concentration was measured using a Coomassie (Bradford ...
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bioRxiv - Plant Biology 2020Quote: ... and 10 μCi [γ32P]-ATP in reaction buffer (25 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 0.5 mM CaCl2, 2 μM CaM [Sigma-Aldrich], 1 mM DTT) at 30°C for 30 min to perform the vitro kinase assay ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mM Tris/HCl pH 8.2) and then loaded to regenerated cellulose centrifugal filter units (Microcon 30, Merck Millipore, Billercia MA, USA). Samples were spun at 14000 g at 35°C for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and the cells were lysed in TX-100 lysis buffer (300mM NaCl; 50mM Tris-HCl, pH 7.5; 0.5% Triton X-100; β-mercaptoethanol, Sigma protease inhibitor cocktail) for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... sacculi were centrifuged and redissolved in 20 ml of Tris-HCl buffer (50 mM, pH 7.6) with 2 mg Trypsin (Sigma-Aldrich, T-4799). Trypsin digestion was performed overnight at 37 °C under constant shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining ‘strong’ bound phages are then eluted from the surface in a stepwise manner by applying an elution buffer consisting of 0.2 M Glycine-HCl pH 2.2 (Sigma Aldrich, St. Louis, MO) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... The dried polar metabolite pellet was derivatized by a two-step methoxyamine protocol first by addition of 70μL methoxyamine HCl (Sigma-Aldrich #226904-5G) in pyridine (20 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins from the ground protoplasts were extracted in 500 µl of protein extraction buffer (150 mM NaCl, 50 mM Tris HCl, pH 7.5, 0.1% Nonidet P-40, and 1% protease inhibitor cocktail [Sigma-Aldrich, Catalog No.: P2714]) and centrifuged for 5 min at 10,000 rpm and 4°C ...