Labshake search
Citations for Millipore Sigma :
601 - 650 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 µm Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... The cell pellet was resuspended in 100 μl of Proteinase K extraction buffer [40 mM Tris-HCl (pH 8.0) (Sigma), 1% Tween-20 (Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... Thawed nuclei were resuspended in sonication buffer (50mM Tris-HCl, 1% SDS, 10mM EDTA, 1x protease inhibitor (Sigma-Aldrich), pH 8.0 ...
-
bioRxiv - Neuroscience 2022Quote: ... The other homogenization buffer was CST buffer (NaCl2 146 mM, Tris HCl pH 7,5 10 mM, CaCl2 1mM, MgCl2 21 mM, 0.49% CHAPS (Millipore-Sigma), 0.01% BSA ...
-
bioRxiv - Developmental Biology 2022Quote: ... lysed them in NP40 lysis buffer (50mMTris-HCl, 150mMNaCl, 0.1% NP-40, 5mM EDTA, 10% glycerol) with protease inhibitors cocktail (Sigma), and sonicated using a Bioruptor (Diagenode) ...
-
Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 2 mM MgCl2 and protease inhibitor cocktail [Sigma]). 10% of lysate volume was reserved to determine total protein amounts ...
-
bioRxiv - Molecular Biology 2021Quote: ... to 7.0 with HCl) for 45 minutes and a final wash in in 2x SSC (3 M NaCl (Sigma), 0.3 M sodium citrate (Sigma) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested in TENT buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μl TUBEs beads/IP were washed twice with TBST (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween 20; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
-
bioRxiv - Genomics 2020Quote: ... cells were lysed on ice in 5 ml lysis buffer (10 mM Tris-HCl, pH 8, 10 mM NaCl, 0.2% Igepal NP-40 (Sigma), 1× cOmplete protease inhibitor (Roche ...
-
bioRxiv - Genomics 2020Quote: ... The crosslinked pellets were washed with 1 x PBS and incubated with 200ul of lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630, 33 μL Protease Inhibitor (Sigma, P8340)) on ice for 15 min ...
-
bioRxiv - Genomics 2020Quote: ... at 33°C for 10min (mouse brain) and 60min 75μl 0.1% pepsin (spleen) prepared at pH 2.5 in Tris-HCl (Sigma-Aldrich, USA). After a 180μl 0.1X SSC wash at 33°C ...
-
bioRxiv - Genomics 2020Quote: ... Nuclei were extracted according to81 using the cell lysis buffer (10 mM Tris–HCl pH 7.5, 10 mM NaCl, 0.5% IGEPAL (CA-630, Sigma, I8896) and a Dounce homogenizer.
-
bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were permeabilized in 1ml lysis buffer (10mM Tris–HCl pH 8, 10mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Sigma) for 15 min on ice ...
-
bioRxiv - Cell Biology 2019Quote: ... thrombin cleavage were conducted in standard buffer (20 mM Tris-HCl, pH 7.4, 200mM NaCl) with 0.03 U/μl of thrombin (Sigma-Aldrich) at 4°C for 6 hours.
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed in modified Laemmli buffer (5% SDS, 10% glycerol, 32.9 mM Tris-HCl pH 6.8) supplemented with 1 mM DTT (Sigma-Aldrich), and their extracts (15 µG ...
-
bioRxiv - Developmental Biology 2019Quote: Protein extracts from cells were prepared using a modified Laemmli buffer (5% sodium dodecyl sulphate, 10% glycerol, 32.9 mM Tris-HCl pH 6.8) supplemented with protease inhibitors (Sigma-Aldrich). Brain tissues were prepared with a lysis buffer (Hepes 10 mM ph 7.9 ...
-
bioRxiv - Genomics 2019Quote: ... Pellets of formaldehyde -crosslinked iPSCs were lysed and sonicated in 110 µl of SDS Lysis Buffer (0.5% SDS, 50mM Tris-HCl pH 8.0, 20mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Sigma)) using Diagenode Bioruptor UCD-200 (Diagenode ...
-
bioRxiv - Cell Biology 2019Quote: ... The cells were then resuspended in 1.0 mL homogenization buffer (25mM Tris-HCl pH 7.4, 250 mM sucrose, 1 mM PMSF and protease inhibitor cocktail (Sigma)) and homogenized with a Dounce homogenizer (40 strokes with a tight pestle) ...
-
bioRxiv - Genetics 2019Quote: ... washed and incubated overnight in Tris-HCl 50mM pH 7,5 complemented with RNase A (10 mg/ml; Sigma-Aldrich) at 37°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in 5 µL of cold lysis buffer (10 mM Tris-HCl [pH 7.4], 10 nM NaCl, 3 nM MgCl2, 0,1% v/v Igepal CA-360 [Sigma-Aldrich] and incubated on ice for 5 min ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 ml of alkaline phosphatase (100mM Tris-HCl pH 9.5, 100 mM NaCl, 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma ...
-
bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were buffer exchanged into TBS (20 mM Tris-HCl, pH 7.0 and 150 mM NaCl) by Amicon Ultra-4 Centrifugal Filter Units (Millipore). The final concentrations of the proteins were determined by the BCA method.
-
bioRxiv - Microbiology 2021Quote: ... pellets were resuspended in 2 ml 10 mM Tris HCl (pH 7.4) containing 2 mg ml−1 pronase (Sigma). Tubes were incubated for 3 h at 60 °C to digest proteins ...
-
bioRxiv - Neuroscience 2021Quote: ... to assess protein concentration and then solubilized in 4x loading dye (250 mM Tris-HCl pH 6.8, 40% glycerol, 0.008% bromophenol blue, 8% SDS; all from Sigma-Aldrich). All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad) ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were lysed with modified RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA, 1% NP-40, 0.25% deoxycholic acid; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors ...
-
bioRxiv - Bioengineering 2020Quote: ... Recovered samples were resuspended in 50 mM tris-HCl (pH 8.0) and mixed with equal volumes of 2 mM monobromobimane (mBBr; Sigma). After 15 minutes of incubation in mBBr at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... The culture supernatant containing the target protein was concentrated 3-fold and buffer exchanged against 6 volumes of working buffer (50 mM Tris/HCl, 300 mM NaCl, pH 8.0) by crossflow filtration (Millipore Pellicon 2 mini filter ...
-
bioRxiv - Biochemistry 2021Quote: ... Detergents in the samples were exchanged with 100 mmol/L Tris-HCl (pH 8.5) using Microcon filters (10 kDa cut off, Millipore). Tryptic digestion with trypsin (Sequencing Grade Modified ...
-
bioRxiv - Biochemistry 2021Quote: Frozen helminths were ground with a pestle in a mortar with liquid nitrogen to a powder with the addition of 0.1 mol/L Tris-HCl (pH 7.6) with 1% protease inhibitor cocktail (Amresko) and 1% phenylmethanesulfonyl fluoride (Sigma-Aldrich). After thawing the extraction buffer protein was precipitated with 100% trichloroacetic acid (final concentration 10%)(Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... for 1h at RT, membranes were developed with ECL substrate (10% Tris HCl 1M pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2020Quote: ... samples were washed twice for 5 min with digestion buffer consisting of nuclease-free water with 0.8 M guanidine-HCl (G3272, Sigma), 50 mM Tris-HCl pH 8 ...
-
bioRxiv - Neuroscience 2021Quote: ... hippocampi tissues were manually homogenized in 300 µl ChABC buffer (40 mM Tris-HCl pH 8.0, 40 mM sodium acetate, C3667, Sigma) containing Benzonase supplemented with Halt protease/phosphatase inhibitor cocktail (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were lysed in hypotonic buffer A (10mM Tris-HCl pH 5.5, 500uM DTT, 500uM EDTA, protease and phosphatase inhibitor cocktails (Sigma)) for 10 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were lysed with SDS sample buffer (62.5 mM Tris–HCl, pH 6.8, 2% SDS, 10% glycerol, all from Sigma-Aldrich) without dithiothreitol (DTT ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then pelleted and resuspended in 100–200 µl of lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5 % IGEPAL CA-630 (Sigma-Aldrich), 1 tablet/50 ml cOmplete protease inhibitor cocktail (Sigma-Aldrich)) ...
-
bioRxiv - Cell Biology 2021Quote: ... the coverslips were mounted on microscopy slides with Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Merck Millipore, 81381). The samples were imaged using a Zeiss Axioscope A1 microscope with a 40x objective.
-
bioRxiv - Cell Biology 2021Quote: ... on ice (50mM TRIS-HCl pH 7.4, 150mM NaCl, 10mM iodoacetamide, 2mM PMSF, 1x Cocktail inhibitor from Sigma-Aldrich). Samples were then centrifuged at 4°C for 10 minutes at 1,000g ...
-
bioRxiv - Cell Biology 2021Quote: ... were collected and resuspended in 500μl of extraction buffer (100mM NaCl, 50mM Tris-Hcl pH 8.0, 10mM EDTA, 1%SDS) supplemented with 2μl ß-mercaptoethanol (M6250, Sigma) and 2.5μl of Zymolase 100T (20mg/mL) ...
-
bioRxiv - Microbiology 2020Quote: ... Cell pellets were suspended in 0.3 ml a solution of 10 mM pH 8.0 Tris-HCl and 10 mg lysozyme/ml (Sigma-Aldrich) and incubated at 37°C for 1 hr ...
-
bioRxiv - Systems Biology 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% sodium deoxycholate (Millipore Sigma), 10 mM beta-glycerophosphate (Millipore Sigma) ...
-
bioRxiv - Systems Biology 2020Quote: ... Bacteria were thawed in GST buffer (20 mM Tris-HCl pH 7.5, 0.5 M NaCl, 1 mM EDTA and 1 mM DTT (Millipore Sigma)) with cOmplete protease inhibitors (Millipore Sigma ...
-
bioRxiv - Plant Biology 2021Quote: ... The column was rinsed 4 times with 200 μL TBS (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1x plant protease inhibitor cocktail (Sigma), 20 μM PuGNAc ...
-
bioRxiv - Plant Biology 2021Quote: ... followed by total protein isolation with extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% [v/v] NP-40 and 1:200 protease inhibitor cocktail from Sigma). Co-IP was carried out the same as for co-IP with protoplasts except that GFP-Trap agarose beads (Chromotek ...
-
bioRxiv - Microbiology 2021Quote: ... and applying lysis buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 1% Nonidet P-40 (all from Sigma)) supplemented with EDTA-free protease inhibitor mixture cocktail (Roche) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then incubated in sucrose buffer (0.1 M Tris-HCl pH = 7.5, 2 mM MgCl2, 1 M sucrose, 1 % Protease Inhibitor Cocktail (Sigma Aldrich), 200 μg.mL−1 RNase A and 10 μg.mL−1 DNase (Sigma Aldrich) ...
-
bioRxiv - Systems Biology 2020Quote: ... The equivalent of fifty OD600 of cells were resuspended in ice cold yeast lysis buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM EDTA (Millipore Sigma), 1% triton X-100 (Millipore Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... 0.005 M EDTA pH 8.0, 0, 02 M Tris-HCl pH 8.0, 10 mg ml−1 lysozyme; Sigma-Aldrich) at 37°C for 60 minutes followed by proteinase K solution treatment (7.5 μg ml−1 proteinase K ...
-
bioRxiv - Cancer Biology 2020Quote: ... were loaded with 30 – 50 µg protein in 5X SDS loading buffer (250 mM Tris-HCl, pH 6.8, 10% SDS, 30% glycerol, 0.02% bromophenol blue [Sigma-Aldrich, B8026] ...