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Citations for Millipore Sigma :
751 - 800 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were incubated at 37°C for 10 minutes and then quenched with 0.1M HCl and neutralized with 0.1M Tris base (Trizma base, Sigma-Aldrich). 37.5 µg of snake venom (V7000 ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein sample was then diluted to appropriate concentration in SDS loading buffer (50 mM Tris-HCL pH6.8, 2% SDS (Sigma), 10% glycerol (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... The eluates were pH equilibrated to 7.4 using 1.0 M Tris HCl pH 9.0 and immediately buffer exchanged into DPBS and concentrated using an Amicon ultra centrifugal concentrator (Millipore) with a molecular weight cut-off of 30 kDa.
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bioRxiv - Molecular Biology 2023Quote: ZZZ3 and its interactors were released from the magnetic beads by pre-digestion in 100 µl of digestion buffer (100 mM Tris-HCl pH 8.5, 0.1% Triton X-100, 400 ng trypsin Proteomics Grade (Sigma-Aldrich) for 15 min at 37°C with shaking (500 rpm) ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl [pH 8] and 0.1% (w/v) SDS) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and frozen at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... The assay starts by addition of 100 µl of 0.4% pNP (p-nitrophenyl phosphate in 1 M Tris-HCl, pH 8.0; Sigma-Aldrich) to each sample and incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Unbound target RNA was flushed out with 100 µl T50 and 100 µl imaging buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM Trolox (Sigma), 0.8% glucose ...
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bioRxiv - Genomics 2023Quote: ... Samples were treated with 45 µl of 500 mM HCl and 10% pepsin (P7000; Sigma, 470 St. Louis, MO) at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and 20 µl solution containing 36 mg/ml lithium lactate in 10 mM Tris HCl pH 8.5 (Sigma L2250). Supernatant from uninfected cells and from cells completely lysed with 1% triton X-100 (final concentration ...
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bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
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bioRxiv - Cancer Biology 2023Quote: Tumor tissues were snap frozen upon harvest and homogenized in lysis buffer (Tris-HCl pH 6.8, 62.5 mM; SDS, 2%; protease inhibitor cocktail (Sigma #11873580001); phosphatase inhibitors (Sigma #4906845001) ...
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bioRxiv - Genomics 2024Quote: ... 5-15 x 106 formaldehyde crosslinked cells were lysed and sonicated in 110µl of SDS Lysis Buffer (0.5% SDS, 50mM Tris-HCl pH 8.0, 20mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Sigma)) using Covaris E220 Focused-ultrasonicators (Covaris ...
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bioRxiv - Microbiology 2024Quote: ... and an extract of total cellular proteins was prepared by harvesting the cells by centrifugation and resuspending cell pellets in 50 mM Tris-HCl (pH 8.0) (200 µL per OD unit) and incubated with 5 µg mL-1 lysostaphin (Sigma) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and buffer-exchanged into integrin storage buffer (20mM Tris-HCl pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM CaCl2) using an Amicon Ultra-15 concentrator (Millipore) with a 10kDa cutoff ...
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bioRxiv - Developmental Biology 2023Quote: ... Chromatin lysates were prepared in LB3 (50 mM Tris-HCl pH8.0, 1% SDS, 10 mM EDTA, proteinase inhibitor cocktail (Sigma)) ...
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bioRxiv - Genetics 2023Quote: ... The powder obtained was added to 100 μL chondroitinase ABC solution (50 mM Tris HCl pH 8, 50 mM sodium acetate, 0.1 mU/μL chondroitinase ABC (Millipore)) and incubated in a thermomixer set to 37 °C and 1,500 rpm for 6 h ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Microbiology 2024Quote: ... from the resin was immediately neutralized with 1/10 faction volume of 1 M Tris-HCl pH 8.5 and concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 100kDa NWMCO (Merck Millipore), in which the elution buffer was exchanged with PBS (GIBCO).
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... ADP 15N5 at 0.1 µg/mL (dissolved at 1 mg/mL in 5 mM Tris-HCl, Sigma-Aldrich, 741167), and EquiSPLASH™ LIPIDOMIX at 0.02 µg/mL (prepared as 100 µg/mL ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... ATP 13C10 at 0.1 µg/mL (dissolved at 1 mg/mL in 5 mM Tris-HCl, Sigma-Aldrich, 710695), AMP 13C10 ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... they were washed and eluted with 200 μL of elution buffer (100 mM Tris–HCl pH 8.0, 120 mM NaCl) with 20 mM of L-glutathione reduced (Sigma) freshly added for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 µl solution containing 36 mg/ml lithium lactate in 10 mM Tris HCl pH 8.5 (Sigma L2250). Supernatant from uninfected cells and from cells completely lysed with 1% triton X-100 (final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were diluted with 6 x Laemmli buffer (60% Tris- HCl pH 6.8, 10 % SDS, 30 % glycerol (Carl Roth, 3783) and 0.01 % Bromophenol blue (Sigma, B0126) containing 10 % β-mercaptoethanol (Carl Roth ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in ChIP lysis buffer (50 mM Tris-HCl, pH 7.9, 10 mM EDTA, 1% SDS, 1 mM PMSF, protease inhibitor cocktail (Sigma-Aldrich) on ice for 10 min and were subjected to sonication to shear the chromatin to 200-1,000 bp-long DNA fragments ...
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bioRxiv - Cell Biology 2020Quote: ... in lysis buffer supplemented with phosphatase and protease inhibitors (20 mM Tris-HCl pH 7.5; 150 mM NaCl; 1 % IGEPAL [vol/vol]; Phosphatase inhibitor cocktail 1:100 [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2020Quote: ... cells following subjected treatments were collected and lysed in NP40 buffer (50mM Tris-HCl pH 8, 150mM NaCl, 5mM EDTA, 0.5% NP-40, 1x EDTA-free protease inhibitor (Sigma-Aldrich), 1x phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed (1% SDS, 10 mM Tris-HCl pH 7.5, 1 mM Na3VO4, c0mplete protease inhibitor cocktail (Sigma-Aldrich), heated to 95°C) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected on ice in 0.5% NP40 lysis buffer (50 mM tris-Hcl pH=8. 100 mM NaCl, 10% glycerol, EDTA-free protease inhibitor cocktail (Sigma), 10 μM of MG-132 (Sigma ...
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bioRxiv - Genetics 2021Quote: ... Cleared lysates were adjusted to match the composition of the immunoprecipitation (IP) buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% NP-40 alternative (EMD-Millipore product 492018). The adjusted lysates were combined with anti-VSV-G agarose beads (Sigma ...
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bioRxiv - Genetics 2021Quote: Nuclei were lysed in a high salt buffer (20mM Tris-HCl, pH 7.6, 300mM NaCl, 10% glycerol, 0.2% (v/v) Igepal (Sigma-Aldrich, 630)) and were loaded on a Superose 6 10/30GL column (Amersham ...
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bioRxiv - Genetics 2020Quote: ... Crosslinked cells were resuspended in a pre-chilled lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.2% NP-40) with fresh protease inhibitors (Millipore Sigma). The lysate was then digested with the primary restriction enzyme ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice with TBS 1X and stained for 30 minutes at room temperature using a working solution 2 (WR2) composed by 100mM Tris-HCl pH 8.5 (Sigma Aldrich), 1mM CuSO4 (Merck Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were resuspended in 100 μL anti-fade buffer (0.4 % glucose, 10 μM Tris-HCl pH8, 2x SSC) with 1 μL catalase (Sigma-Aldrich) and 1 μL glucose oxidase (3.7 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... pellets were first thawed on ice and then incubated with 1 ml lysis buffer (10 mM Tris-HCL pH8, 10 mM NaCl, 0.2% Igepal CA-630, Roche Complete EDTA-free Sigma #11836170001). After lysis ...
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bioRxiv - Physiology 2022Quote: ... Membranes were stripped by incubation in 25 mL 0.5 M Tris-HCl / SDS buffer supplemented with 125 μL 2-mercaptoethanol (Sigma-Aldrich) for 1 hour and re-probed.
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bioRxiv - Neuroscience 2021Quote: ... Cell lysis was performed by dissolving the bacterial pellet in 100 mL of buffer A (40 mM Tris–HCl, pH 7.5) containing protease inhibitors [1 mM EDTA (Sigma-Aldrich, cat# 11873580001] and 1 mM PMSF (Applichem ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended in KCl lysis buffer (50 mM Tris-HCl pH 7.5, 225 mM KCl, 1% NP-40) with a protease inhibitor cocktail (Sigma-Aldrich) and other inhibitors (1 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... five fly heads were homogenised in 50 μl GnHCl extraction buffer (5 M Guanidinium HCl, 50 mM HEPES pH 7.3, protease inhibitor cocktail (Sigma, P8340) and 5 mM EDTA) ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) supplemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in non-denaturing lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... to restore osmolarity before being resuspended in 10 mL collagenase buffer (100 mM Tris-HCl, pH=7.4; 1 mM CaCl2) with 1U/mL collagenase (Sigma C0773) and incubated for 2 h at 20°C with gentle agitation ...
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bioRxiv - Molecular Biology 2021Quote: Pellets of cells expressing SFB- or BioID2-tagged viral genes were subjected to lysis with NETN buffer (100mM NaCl, 1mM EDTA, 20mM Tris-HCl, and 0.5% Nonidet P-40) with protease inhibitor cocktail (Sigma-Aldrich) at 4 °C for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... uninfected and SARS-CoV-2-infected Calu-3 cells were lysed in lysis buffer (10 mM Tris/HCl pH 7.4, 150 mM NaCl, 1% Igepal CA630 (Sigma-Aldrich) and 1x cOmplete™ Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Genomics 2020Quote: ... cells were cross-linked with 1% fresh formaldehyde for 15 minutes and treated with lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% IGEPAL CA-630 (Sigma-Aldrich), 1X complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell pellet was resuspended in 1 mL of strep-Tactin lysis buffer (50 mM Tris HCl pH 8.0, 150 mM NaCl) containing protease inhibitors cocktail (1x concentration, cOmplete ULTRA, Sigma-Aldrich) and RNase A (0.1 mg/ml ...
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bioRxiv - Plant Biology 2019Quote: ... in protein sample buffer (50 mM Tris HCl [pH 7.5], 10% glycerol, 2% SDS, 2 mM EDTA, 1 mM DTT and 1% protease inhibitor [Sigma-Aldrich]). Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... pellets were resuspended in 50 μL of lysis buffer (20 mM Tris-HCl pH 7.5, 1 mM EDTA, 0.5 mM Na3VO4, 1 mM benzamidine hydrochloride (Sigma-Aldrich, Germany), 5x cOmplete™ EDTA-free protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...