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Citations for Millipore Sigma :
951 - 1000 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and lysed in solubilization buffer (50 mM Tris–HCl pH 8, 1% Igepal and 1 mM EDTA) with protease inhibitor cocktail (Sigma Aldrich). The cell lysate was centrifuged at 14,000×g for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... STORM imaging was performed in fresh imaging buffer (50mM Tris-HCl, pH 8.0, 10mM NaCl, 10% (w/v) glucose (Sigma, cat#G8270), 1.5mg MEA (Sigma ...
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bioRxiv - Genomics 2020Quote: Cells growing in log phase were harvested for protein extraction using the protein extraction buffer (50 mM Tris–HCl, 150 mM NaCl, 1% Triton-X, 10% glycerol, 5 mM EDTA; all Sigma-Aldrich) and 0.5 μl protease inhibitor mix (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysis was performed by dissolving the bacterial pellet in buffer A (20 mM Tris–HCl, pH 7.5) containing protease inhibitors (1 mM EDTA (Sigma-Aldrich, 11873580001) and 1 mM PMSF (Applichem ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Cell Biology 2021Quote: Cells treated with SPS either alone or in conjunction with bafilomycin-A1 were collected and subsequently lysed in lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40) supplemented with PhosStop phosphatase inhibitor cocktail (Sigma Aldrich) and protease inhibitor cocktail (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK293T cell lysates transiently expressing mScarlet-I-tagged spartin truncation mutants were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... were homogenized using a glass homogenizer, in cold lysis buffer (RIPA buffer: 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, Complete protease inhibitor (Millipore Sigma, Burlington ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in urea buffer (50mM Tris HCl pH 7.5, 5mM EDTA, 6M Urea, 1% SDS, 1mM PMSF, 1:100 Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich P0044]) and cells were lysed by bead beating ...
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bioRxiv - Plant Biology 2021Quote: SDS washed and de-starched dry onion peels were weighed and added to a 2 mL screw – top microcentrifuge tube and 1 mL of 3 M HCl in methanol (Sigma, USA) was added to the walls and incubate for 16 h at 80°C in heating block in a fume hood as per De Ruiter et al ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Plant Biology 2021Quote: ... roots of Arabidopsis thaliana Col-0 and sr45-1 mutant seedlings (or 9-week-old plants) were vacuum infiltrated with HCl (4%, v/v) and K-Ferrocyanide (II) (4%, w/v, Sigma-Aldrich) (1/1 ...
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bioRxiv - Cancer Biology 2020Quote: ... were loaded with 30 – 50 µg protein in 5X SDS loading buffer (250 mM Tris-HCl, pH 6.8, 10% SDS, 30% glycerol, 0.02% bromophenol blue [Sigma-Aldrich, B8026], 5% 2-mercaptoethanol [Sigma-Aldrich, M6250]) after heating the samples for 5 min at 95°C ...
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bioRxiv - Biophysics 2022Quote: ... The oligonucleotides were dissolved at 100 μM in 10mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA purchased (Merck Millipore, MA, USA) and stored at −20 °C for further use.
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bioRxiv - Cell Biology 2022Quote: ... and resuspended in a small volume of PBS supplemented with protease inhibitors (50X stock contains: Benzamidine HCl 800 μg/ml (Sigma #B65506), Aprotinin 500 μg/ml (Sigma #A1153) ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfected cells were lysed for 20 minutes in pre-chilled Co-Immunoprecipitation buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.53% NP-40) supplemented with 1×protease inhibitor (Roche-Sigma, Cat no. # 11873580001) and 1×phosphatase inhibitor (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated in the dark for 30min with SDS buffer (100 mM Tris–HCl pH 8, 1% SDS, 250 mM NaCl, 5 mM EDTA) with 40 mM iodoacetamide (Sigma, #I1149). The resin was then resuspended in SDS buffer ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of supernatant from cultures incubated with 50 μM or 150 μM of the compounds for 4 hours or 8 hours was mixed with 1 ml of 30 mM Tris-HCl buffer containing 10 mg/ml of Elastin-Congo Red (Sigma-Aldrich). The mixture was then incubated with shaking at 37°C for 1–2 hours ...
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bioRxiv - Neuroscience 2020Quote: Cell lysates from LM fibroblasts were harvested in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris-HCl) with 1% protease inhibitor cocktail (Sigma, P8340), 3 days after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... 100mM Tris/HCl pH 8.2) and then loaded to regenerated cellulose centrifugal filter units (Microcon 30, Merck Millipore, Billercia MA, USA). Samples were spun at 14000g at 35°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... The sample was washed twice using 50 mM Tris HCl containing 25 mM NaCl at pH 8.5 and 0.5% w/v low gelling agarose solution (Sigma-Aldrich, #A9414-100G) was carefully added to the top to ensure proper immobilization ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 24 h at 60 °C in papain digestion solution containing 125 μg/ml of papain with 5 mM L-cysteine-HCl and 5 mM EDTA in 100 mM Na2HPO4 (all from Sigma, USA). The DNA content was measured using the Picogreen assay ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Genomics 2019Quote: ... Nuclei were isolated by incubation of 107 cells for 10 min on ice with 5 ml RSB buffer [10 mM Tris–HCl (Sigma, T2663) pH 7.4 ...
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bioRxiv - Genomics 2019Quote: ... The cells were resuspended in 500 μl of ice-cold lysis buffer (10 mM Tris-HCl, pH8.0, 10 mM NaCl and 0.2% (v/v) Igepal CA-630 (Sigma, 18896-100 ML)) containing 1x proteinase inhibitor complex (PIC ...
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bioRxiv - Cancer Biology 2020Quote: ... collected and resuspended in lysis buffer (30 mM Tris-HCl, 150 mM KCl, 10 mM MgCl2 supplemented with DDT (Sigma-Aldrich) 1 mM ...
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bioRxiv - Genetics 2019Quote: ... Crosslinked cells were lysed for 10 min on ice in 50 μl of lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1mM EDTA, 1% SDS) freshly supplemented with proteinase inhibitor (Sigma-Aldrich). IP dilution buffer (20 mM Tris-HCl pH8 ...
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bioRxiv - Microbiology 2019Quote: ... Tube 1 was extracted again by adding 200 µl of TE buffer (10 mM Tris-HCl, 1 mM disodium salt of EDTA, pH 8.0, Sigma Aldrich Co.), vigorously mixing for 1 min and phase separation at 12,000 g for 5 min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... rinsed with 3 mL of medium and washed off into new Eppendorf tubes with 600 μL of boiling buffer (100 mM Tris-HCl, 4 mM EDTA, pH 7.5, autoclaved and supplemented with phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726)) ...
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bioRxiv - Genomics 2019Quote: ... Cells were washed once with ice-cold PBS and gently resuspended in 300 μl RSB [10 mM Tris HCL (Sigma, T2663) pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: Dry samples were resuspended in 300 µL 0.1 N HCl and filtered through 0.45 micron filters (EMD Millipore, Billerica, MA, USA). 5 µL of the filtered extracts was derivatized according to the AccQTag™ Ultra Derivitization Kit protocol (Waters Corporation ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... brain slices were first incubated in a 2N-HCl for 45 minutes at 45°C and then rinsed in a boric acid (Sigma-Aldrich) solution 0.1 M at pH= 8.5 for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... the fixed samples were lysed (lysis buffer: 10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% Igepal CA-630 (Sigma-Aldrich, I8896) and 1× protease inhibitor cocktail (Complete ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4 (prepared using 25% glutaraldehyde solution G5882, Sigma-Aldrich; sodium cacodylate trihydrate C0250, Sigma-Aldrich; pH adjusted with 0.1M HCl) for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... pulverized in liquid nitrogen and protein was extracted using a 1x radioimmunoprecipitation assay buffer (50mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Nonidet®40 Substitute (74385; Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Cell Biology 2022Quote: ... samples were lysed in 1% Triton X-100 Buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100) supplemented with protease inhibitors (Sigma-Aldrich #11836170001). Lysates were sonicated ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Genomics 2022Quote: ... Cell lysis was performed by resuspending the pellet in 500 μl Cell Lysis Buffer (Final 10mM Tris-HCl, pH 8,0 (Sigma Aldrich, T2694); 85mM KCl (Sigma Aldrich ...
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bioRxiv - Genomics 2022Quote: ... twice with LiCl Wash Buffer at 4°C (Final 10 mM Tris-HCl, pH 8,0; 1mM EDTA; 250mM LiCl (Sigma Aldrich, L9650); 1% sodium deoxycholate (Sigma Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was carefully removed and the extracted nuclei were then resuspended in 230 μl Nuclei Lysis Buffer (Final 10mM Tris-HCl, pH 7,5 (Sigma Aldrich, T2319)) ...
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bioRxiv - Genomics 2022Quote: ... bead/chromatin complexes were washed twice with Low Salt Wash Buffer at 4°C (Final 20 mM Tris-HCl, pH 8,0; 2 mM EDTA; 150 mM NaCl (Sigma-Aldrich, S6546); 1% Triton-X ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... catalog no. AB01922) was purchased from American Bio and Guanidine hydrochloride (G-HCl; catalog no. G3272) was purchased from Sigma-Aldrich
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bioRxiv - Cell Biology 2022Quote: ... and homogenized using a Potter-Elvehjem tissue grinder on ice in ice-cold incomplete radioimmunoprecipitation assay buffer (RIPA) containing 50 mM Tris-HCl pH 7.4 (Sigma-Aldrich, T1503) and 100 mM NaCl (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... the typical reaction mixture included 3 μg of ATG3 in 50 mM Tris HCl (pH 7.5) in the absence or presence of reducing [DTTred (Sigma-Aldrich; 43815) or GSH (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Cells were collected and lysed using Lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP40 supplemented with Halt protease inhibitor cocktail (Sigma, S8830-20Tab). Samples were incubated for 2 hours with gentle rotation at 4 °C and then centrifuged at 20,000 g for 20 min ...