Labshake search
Citations for Millipore Sigma :
8701 - 8750 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... we extracted single cell suspensions from the homogenized spleens of 3 wild-caught PUUV-seronegative bank voles by washing the homogenate through 70 µM cell strainers (Sigma Aldrich) with 10 ml PBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... ChIP and input samples were incubated for 2 hours at 37°C with 0.2 mg/ml RNAse A (Millipore 70856-3), and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
-
bioRxiv - Developmental Biology 2021Quote: ... 500 μg of each channel was combined for buffer exchange on a 3 kDa molecular weight cut-off column (EMD Millipore) into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 μl lipids and 3 μl standards (30 μg mono-, di-, triglyceride mix, SUPELCO 1787-1AMP, plus 30 μg cholesteryl palmitate, SIGMA C6072) were spotted onto the plates using TLC spotting capillaries ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 5 µL of cells were transferred into 100 µL of staining solution (Phosphate Buffer Saline + 3 µM propidium iodide (Sigma-Aldrich) + 200 nM YP (Invitrogen) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The plates were incubated for two hours at RT and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate solution (Sigma-Aldrich, USA) was added for colour development ...
-
bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
-
bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
-
bioRxiv - Microbiology 2022Quote: ... the samples were infiltrated with 70% Epon in Ethanol before two incubations with 100% Epon and the final polymerization was carried out in Epon supplemented with 3% silver flakes (Sigma-Aldrich) and 3% (w/w ...
-
bioRxiv - Biochemistry 2022Quote: ... and 3% hydrochloric acid followed by sample pooling via centrifugation at 1,500 rpm for 2 minutes to the proteoCHIP funnel part for direct injection or transferred to 0.2 mL PCR-tubes coated with 1e-3 % Poly(ethylene glycol) (95172-250G-F, Sigma-Aldrich, Germany).
-
bioRxiv - Cell Biology 2022Quote: ... To induce the auxin-degron system in DLD-1 cells, 500 µM indole-3-acetic (A10556, Fisher) and 500 µg ml−1 doxycycline (Sigma, D9891) were added to the media and cells incubated for 24 h.
-
bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
-
bioRxiv - Neuroscience 2021Quote: ... Each well of 6-well cell culture treated plates coated with 0.1% Poly-L-ornithine (3 ug/mL; Sigma Aldrich, P4957) were seeded with ∼750K cells and maintained with sterile-filtered neurobasal medium (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Brain tissue is then washed twice with 2-3 mL sterile-filtered DM+Trypsin Inhibitor (1 mg/mL; Sigma Aldrich, T9253) and incubated in the 3rd wash for 3-5 min at 37° C ...
-
bioRxiv - Neuroscience 2021Quote: ... permeabilized in PBS containing 0.5% Triton X-100 (Tx-100; Nacalai Tesque) for 30 min and blocked in PBS containing 3% bovine serum albumin (BSA; Sigma-Aldrich) and 0.05% Tx-100 for 45 min ...
-
bioRxiv - Neuroscience 2020Quote: ... 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001; Protease Inhibitor, Sigma-Aldrich, #P0044) by homogenizing 3 x 20s and sonicating on ice for 15s at 40Hz ...
-
bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... The gradient was fractionated from the top in 11-12 fractions using wide-bore pipette tips and peak 2-3 fractions were identified by immunoblotting against γ-tubulin with GTU-88 antibody (Sigma). γ-TuRC was concentrated to 80 μl in 30k NMWL Amicon concentrator (EMD Millipore ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... After inducing the expression of the different Taf8 transgenes for 3 days with 1 μg/ml doxycycline (Dox, D9891 Sigma-Aldrich) positive cell clones were selected by western blot assays using an anti-Flag antibody ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with PBS-G and blocked in PBS-G with 0.5% fish skin gelatin (Sigma-Aldrich, G7041) for 20 minutes at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... the samples were washed in PBS at least 3 times for 4 hrs and then incubated in Histodenz-Triethanolamine solution (about 80% Histodenz D2158 (Sigma-Aldrich), 11.5 mM PB ...
-
bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
-
bioRxiv - Cell Biology 2019Quote: ... and lysed on ice for 20 min in lysis buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.4], 5 mM EDTA, 1% Triton X-100, 3% glycerol [Sigma-Aldrich, G6279]) with a protease inhibitor cocktail (Roche ...
-
bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
-
bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
-
bioRxiv - Biophysics 2019Quote: ... Blots were then washed 3 x 5 mL TBS-T and incubated with secondary antibody (EMD Millipore, Cat #AP307P, 1:2500) for 1h at 25°C ...
-
bioRxiv - Biochemistry 2019Quote: ... half of the dishes (3 full 15cm dishes per sample) were treated for 10 minutes with diamide (250 µM) (Sigma Aldrich) and subsequently harvested for immunoprecipitation with FLAG beads as described before ...
-
bioRxiv - Genetics 2019Quote: ... cells on coverslips were fixed in 4% PFA for 20 min and blocked with 3% BSA in PBS for 10 min before applying Ret-P1 anti-rhodopsin antibody (Sigma #O4886) at a final concentration of 1:1000 in blocking buffer for 1 hr ...
-
bioRxiv - Genetics 2019Quote: Total RNA was isolated from healthy wandering third instar larvae of wild type and delayed third instar larvae from homozygous l(3)tb mutant using TRIzol reagent following the manufacturer’s recommended protocol (Sigma-Aldrich, India). The samples were incubated with 2U of RNase-free DNaseI (MBI fermentas ...
-
bioRxiv - Molecular Biology 2019Quote: ... in second attempt we used the library consisted of 8 random nucleotides with fixed G in the middle position: 5’-GCTCAAGCTTACTGCAGATATNNNNGNNNNTTTAGGATCCATCTAGAGTCCGA- 3’(SIGMA-ALDRICH). 15µg of the library were made double-stranded with 5µg 3’ primer (CAST1_R- ACTCTAGATGGATCCTACG or CAST2_R-TCGGACTCTAGATGGATCCTA ...
-
bioRxiv - Microbiology 2019Quote: ... Embryos were maintained at 28.5°C in egg water containing 60 µg / ml Tropic Marin Sea Salt (Norwood Aquarium) and anaesthetised with egg water containing 200 µg / ml buffered tricaine (3-aminobenzoic acid ethyl ester) (Sigma-Aldrich) during bacterial injections and imaging ...
-
bioRxiv - Developmental Biology 2019Quote: ... Western blots were incubated with the following primary antibodies for 1 hour at room temperature in 3% milk: 1:4000 M2 Flag (Sigma 3165),1:4000 H3 (Cell Signaling 4499) ...
-
bioRxiv - Microbiology 2020Quote: ... Infected cells were treated with the indicated concentrations of caffeine (RyR, 0-200 mM) or IP3 (IP3R, 0-3 µM) (Sigma-Aldrich). A Synergy (BioTek ...
-
bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
-
bioRxiv - Microbiology 2019Quote: ... 5’-CTGGGAGTATACTTGTAGTC-3) were run in multiplex with PCR reaction mixtures consisting of 10 μl redtaq (REDTaq ReadyMix PCR Reaction Mix, Sigma-Aldrich), 2 μl DNA ...
-
bioRxiv - Neuroscience 2019Quote: ... and slices were incubated overnight at 4 °C in a PBST-based antibody carrier solution containing 3% normal goat serum and rabbit anti-TPH2 primary antibodies (Millipore, ABN60) diluted 1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... for all cell experiments cells were seeded at 3×10^5 cells in 1.5 mL of media onto 10 µg/mL fibronectin (Sigma-Aldrich, F1141) coated MatTek dishes (MatTek Corporation ...
-
bioRxiv - Biophysics 2019Quote: ... We diluted the nanorods 1:3 in single molecule clean phosphate-buffered saline (PBS, P4417 Sigma-Aldrich, St. Louis, MO, USA), sonicated them for 5–10 min ...
-
bioRxiv - Genetics 2019Quote: ... 1000 μg whole cell lysates were incubated with the indicated anti-Flag M2 affinity gel and monoclonal anti-HA agarose for 3-4 hr at 4 degree (Millipore Sigma). Immunoprecipitants were washed five times with NETN buffer (20 mMTris ...
-
bioRxiv - Bioengineering 2019Quote: ... Inks consisted of particles suspended in 1% alginate or pristine alginate (CAS 9005-38-3, Sigma Aldrich, St. Louis, MO, USA) solutions.
-
bioRxiv - Cell Biology 2020Quote: ... BI-D1870 (called ‘p90RSKi’ in this study, Cayman 15264), BIO-6-bromoindirubin-3′-oxime, aka BIO (called ‘GSK3i2’ in this study, (Sigma B1686), BI 2536 (called ‘PLKi’ in this study ...
-
bioRxiv - Cancer Biology 2019Quote: Media was conditioned with melanoma cells for 24-72 hours and concentrated 10x using Amicon Ultra 3-kDa Concentrators (Millipore Z740169). Amyloid Beta-40 ELISA was performed using the Human AB40 ELISA Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2019Quote: ... Each sample was then treated with 1% 3-(N,N-dimethyltetradecylammonio)propanesulphonate (Sigma-Aldrich; in 100 mM citric acid, pH 2.0) at 50°C for 20 min ...
-
bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
-
bioRxiv - Cell Biology 2019Quote: ... fragments of NUP153 (AAS 228-611) were fused C-terminally to GST in pGEX4T-3 and expressed in BL21 DE3 cells (Sigma CMC0014). Cell pellets were lysed (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Physiology 2019Quote: ... and cultured for four days in 100 μL growth media (αMEM, 3 mg/mL BSA, 1 mg/mL bovine fetuin (Sigma, F3385), 0.01 IU/mL recombinant human FSH (NHPP ...
-
bioRxiv - Microbiology 2019Quote: ... Medium was changed the following day to standard media supplemented with 3 μM pyrimethamine and 10 μg/mL DNaseI (Sigma-Aldrich). At each passage of the screen ...