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Citations for Millipore Sigma :
8501 - 8550 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... To the bead suspension was added 0.5 mL of 750 mM of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in water (Sigma-Aldrich) and the mixture was incubated for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... Splenocytes were incubated for 1 hour at 37°C in 96-well plates in RPM11640 medium (3 ml) (Sigma-Aldrich) with or without EPO (35U/well ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed in TBS (3 washes for 5 min each) and then treated with 0.25% H2O2 (Cat#216763, Sigma-Aldrich), in TBS for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Product number E4250) (1 mg/ml) to contract pigment cells alongside 0.01% Tricaine (Ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich (Merck) Product number E10521 ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Microbiology 2023Quote: The small interfering RNAs (siRNAs) against NLRP3 (siNLRP3) (5’-UGCAAGAUCUCUCAGCAAA-3’) and the corresponding control siRNAs (siControl) (5’-UUCAAUAAAUUCUUGAGGU-5’) were synthesized from Sigma. The siGenome smart pool siRNAs and siON Target plus siRNAs were purchased from Dharmacon and are composed of a pool of four siRNAs ...
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bioRxiv - Microbiology 2023Quote: ... A 4 µL sample of the stock suspension of latex beads (containing about 3×1010 mL-1 of 0.3 µm diameter beads, Sigma-Aldrich) was added to each protein and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed again 3 x 5min with ddH2O at room temperature and incubated in thiocarbohydrazide (TCH, 0.22mm Millipore filtered) solution for 20 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Developmental Biology 2022Quote: ... The wells were washed with ddH2O for 3 times and incubated with 100ul/well of 10μg/ml fibronectin bovine plasma (Sigma-Aldrich) in 0.1% gelatin for 2 hours on an orbital shaker at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Microbiology 2023Quote: ... they were washed 3 times with 1M sorbitol and resuspended in RPMI 1640 broth containing protease inhibitor cocktail (Sigma-Aldrich) to final concentration of 3×108 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were subsequently washed 3 × 5 min in 1x TBS/ 0.1% Tween-20 and developed using Immobilon Western HRP Substrate (Millipore, WBKLS0S00) and imaged using the Azure C300 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Cancer Biology 2023Quote: ... Floxing of the transgenic 3’ kappa enhancer was achieved by treating tumor bearing mice with 1 mg tamoxifen (Sigma-Aldrich) in corn oil given by daily i.p ...
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bioRxiv - Immunology 2023Quote: ... Larvae were anaesthetized in zebrafish water supplemented with 200 μg/ml tricaine (Ethyl 3-aminobenzoate methanesulfonate, MS-222 Sigma #A5040) before any manipulation (infection or imaging ...
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bioRxiv - Bioengineering 2023Quote: ... and ligation using T4 DNA ligase (NEB at 16 °C for 16 hours with a 1:3 molar ratio of insert to vector. Ligations were dialyzed on Millipore VSWP 0.025 μm membrane filters for 90 minutes before electroporating (2mm cuvettes at 2.4kV ...
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bioRxiv - Genomics 2023Quote: ... Twenty-four hours after transduction cells were recovered for another 24 hrs and then MCF10A were selected in 3 μg/ml puromycin dihydrochloride (Sigma) for 48 hrs or 10 μg/ml blasticidin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Immunology 2023Quote: ... capsules were attached to the back of mice using a warmed adhesive solution made from 3 parts gum rosin (Millipore- Sigma) and 1 part beeswax (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and immunoprecipitates were analyzed on Western blots using mouse monoclonal anti-MYC antibody (1:800; Covance) (for exogenous 14-3-3ε) or anti-FLAG antibody (1:800; Sigma) (for exogenous Cdc25B ...
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bioRxiv - Microbiology 2023Quote: ... The peak absorbance (A280) fraction was collected and concentrated to 22 mg/mL using a 3 kDa MWCO concentrator (Millipore) before flash freezing in liquid nitrogen.
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
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bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
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bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was first precoated with fibronectin (3 µg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report73 ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were washed again carefully with PBS 3 x 5 mins and the coverslips were mounted onto glass microscope slides with Fluoroshield with DAPI (Sigma) and sealed with clear nail varnish ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting conjugated antibodies are then washed for 3 times in high salt PBS (0.9 M NaCl) in a 50 kDa centrifugal column (Sigma, UFC505096), buffered with at least 30% v/v Candor Antibody Stabilizer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Penis and lymph nodes were dissected and fixed for 1.5-2 h in 3% paraformaldehyde (penis) or overnight in 1% paraformaldehyde (PFA, Sigma, P6148) at 4°C (lymph nodes ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were spun down and the supernatant was passed through a C8 membrane (3 M Empore™, Sigma-Aldrich), to remove any residual beads ...
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bioRxiv - Cell Biology 2023Quote: ... Dissected gut segments were inverted inside out and incubated in DMEM supplemented with 3 mM DTT (Sigma Life Science, D9779), 1 mM EDTA (Gibco ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Molecular Biology 2023Quote: ... TOE1 was depleted in HEK 293T-REx-derived TOE1-degron cells12 by incubation with 600 μM auxin hormone Indole-3-Acetic Acid (IAA, Sigma) for 8 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked with 3% BSA/PBS for 1 hour and incubated with rabbit anti-Olig2 (1:500, Millipore), mouse anti-CC1 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 times for 5 min in PBS and blocked with 3% BSA in PBS-Triton 0.01% (Triton X-100, Sigma, T8787) for 1h ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Plant Biology 2023Quote: 15 dpi nodules were hand sectioned and kept for 10 minutes in staining solution (1:3 ratio of 1:500 dilution of propidium Iodide (Sigma) and 1 ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were washed 3 times in PBS-T (phosphate buffered solution + 0.6% Triton-X 100) (Sigma-Aldrich, Cat. No. X100). PBS-T was removed and VECTASHIELD Mounting Media (Vector Labs ...
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bioRxiv - Pathology 2023Quote: ... Nuclear fast red solution was prepared using 0.1 g nuclear fast red powder in 100 mL distilled water with 5 g Al2(SO4)3 (Sigma-Aldrich). Deparaffinized slides were washed with distilled water and then incubated with the Alcian blue solution for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...