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Citations for Millipore Sigma :
8901 - 8950 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed two more times with PBS (Biotech, PD8117) then blocked and permeabilised using a 3% BSA (Sigma-Aldrich, 1073508600), 0.2% Triton X-100 (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Molecular Biology 2019Quote: ... HeLa cells were treated with 60 μM of the adenosine analogue 5,6-dichloro-1-3-D-ribofuranosylbenzimidazole (DRB; Sigma-Aldrich®) to inhibit transcription and RNA was extracted at different time points for further RT-qPCR analysis.
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bioRxiv - Bioengineering 2019Quote: ... Scaffolds were thoroughly washed 5 times with water and then incubated with 4mg/ml N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich) and 10mg/ml N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were washed 3 times for 5 min in PBS and then cover-slipped and mounted using Fluoroshield mounting medium with DAPI (Sigma Aldrich). Stained sections were imaged on a Zeiss Axio Observer wide-field fluorescence microscope.
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bioRxiv - Synthetic Biology 2019Quote: ... At 1 h post inoculation (time 0 in Figure 3) each flask was added with the stated amount of L-arabinose (Millipore Sigma): 0% ...
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bioRxiv - Microbiology 2019Quote: ... The cells were maintained in DMEM supplemented with 10% FBS for 3 days and then the cells were incubated with DMEM supplemented with 10% FBS and 5 μg/ml puromycin (Sigma-Aldrich) for 4 days to allow the selection of cells expressing the gene of interest.
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bioRxiv - Cell Biology 2019Quote: ... 100 nM ALK2/3 inhibitor LDN193189 (Miltenyi Biotech, 130-106-540) and 10 uM ALK4/5/7 inhibitor SB431542 (Millipore, 616464). Medium was replaced on days 1 and 2.
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bioRxiv - Pathology 2019Quote: ... all drug treatments (1 μM Cysteamine; Sigma, 100 nM Everolimus; RAD001 Selleckchem, 30 mM 3-methyladenine; Sigma, 50 mM Sucrose; Sigma) were added to cell culture medium and incubated with the cells for 24 hrs.
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bioRxiv - Microbiology 2019Quote: ... The dilutions (10−3 to 10−8) were plated in triplicates on LB plates supplemented with nystatin (Sigma, 100 μg/ml) to prevent fungal growth ...
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bioRxiv - Biophysics 2019Quote: ... Of each lipid 10µl at 3mM dissolved in chloroform/methanol (3:1) were deposited in a glass plate coated with indium tin oxide with about 100Ω resistivity (Sigma-Aldrich) and placed 60 minutes in the desiccator for complete solvent evaporation ...
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bioRxiv - Immunology 2019Quote: C57BL/6 mice (female, 9-10 weeks old) were nebulised with LPS (3 mg per group of 8 mice) (Pseudomonas aeruginosa, Sigma-Aldrich) and immediately injected intraperitoneally (i.p. ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50,000 live cells were spun down and incubated 3 min at 4oC in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma-Aldrich, St.Louis, MA), 0.2% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... 8- to 10-week-old body weight- and gender-matched mice were treated by intramuscular injection of 3- methylcholanthrene (MCA, Sigma-Aldrich) mixed with corn oil (5mg/ml ...
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bioRxiv - Cancer Biology 2019Quote: 5′end biotin-labeled TP53 3′UTR RNA fragments with different PAS and other mutations and miRNA of interest labeled with biotin at the 3′end were commercially synthesized (Sigma-Aldrich). H1299 cells were seeded one day before transfection in 10 cm tissue culture dish at 50% confluent ...
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bioRxiv - Microbiology 2020Quote: ... Cleaned tissue samples were incubated at 37 °C for 1 h in 10 mL DPBS with 1 mM DTT and 3 mM EDTA (Sigma-Aldrich). After incubation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and Griess reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Penicillin ...
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bioRxiv - Pathology 2019Quote: ... six week old Il11SMC mice were intraperitoneal injected with 3 doses of 50 mg kg-1 tamoxifen (tam; T5648, Sigma Aldrich) or an equivalent volume of corn oil vehicle (veh ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with 50 μM of the Arp2/3 inhibitor CK-666 or its control analog CK-689 (Sigma-Aldrich) for 1 hour prior ...
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bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation (further desalting) was performed on a Discovery® BIO Wide Pore C5 column (100 × 2.1 mm, particle size 3 μm, Sigma-Aldrich) at 55 °C with a mobile-phase system consisting of water and acetonitrile (each containing 0.1% formic acid) ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... and after 3-5 days transplanted into pots containing autoclaved vermiculite and sterile 0.5 × Murashige and Skoog (MS) medium (Sigma Aldrich, USA) with no carbon supplement ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 3% BSA in PBS-T buffer for 1 h at room temperature followed by incubation with Streptavidin-HRP (Sigma-Aldrich) (1/25,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... the grid was manually blotted and 3 µL of 0.3 mg/mL heparin from porcine intestinal mucosa (Millipore, Cat. No. 375095) applied and incubated for 2 min at 22 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were dispersed with Accutase at d45 of differentiation and plated at a concentration of 3×105 cells/well into MEA plates in 50 μL of culture media containing 10 μg/mL laminin (Sigma, 11243217001). One hour after plating ...
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bioRxiv - Neuroscience 2020Quote: The viability of microglial cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Steinheim, Germany) assay as described previously [36] ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Systems Biology 2020Quote: ... 120μL of 1:1 DTNB:GR solution (DTNB: 2mg 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich; Cat#D8130; CAS: 69-78-3) in 3mL KPE ...
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bioRxiv - Molecular Biology 2021Quote: ... the coating solution was discarded and the plate was washed 3 times with 300 µl of wash buffer (1xTBS, 1mM CaCl2, 2mM MgCl2, 0.25% Triton X-100 (Sigma-Aldrich, T8787)) ...
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bioRxiv - Microbiology 2019Quote: Lentiviral particles carrying an anti-CTIF shRNA were produced in HEK293 cells by transfecting a commercially available pLKO.1 vector containing the shRNA sequence targeting the 3’-UTR of the CTIF mRNA (Sigma-Aldrich), pVSVg and psPax2 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti- Spz (rat, 1:50, requested from Satoshi Goto), Anti-Elav (rabbit, 1:50, DSHB) and Caspase 3 (rabbit, 1:500, Sigma Aldrich). Samples were washed four times for 10 minutes each in PBST and incubated at room temperature in secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was sterile filtered through a 0.22 μm 0.5 or 1L vacuum filter and concentrated using the Amicon 200 mL Stirred Cell (UFSC20001) with a 3 kDa NMW Ultrafiltration Disc (Millipore PLBC06210) and Amicon Ultra 15 mL Centrifugal Filters with a 3kDa NMW (Millipore UFC9003) ...
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bioRxiv - Immunology 2019Quote: ... the RAW 264.7 macrophages were washed once with T cell media after which 600 μl T cell media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) was added ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The panel of substances tested included several nucleosides/nucleotides or derivates (ATP, ADP, 5’-AMP, 3’-AMP, cyclic AMP, adenosine, GTP, TTP, inosine, sodium polyphosphate, Sigma-Aldrich), biogenic amines (epinephrine ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Cancer Biology 2019Quote: ... digested with PvuII HF in the proper buffer for 3–5 h at 37 °C and replication intermediates were enriched on a benzoylated naphthoylated DEAE–cellulose (Sigma-Aldrich) column ...
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bioRxiv - Immunology 2020Quote: Urine cells were thawed into 9 ml of 10% FCS-RPMI medium supplemented with 10 IU/ml Benzonase (Novagen 70664-3), counted and viability determined by trypan blue exclusion ...
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bioRxiv - Immunology 2021Quote: Detection of IgA spots was achieved by using anti-IgA-conjugated horseradish peroxidase and developed with 3-amino-9ethylcarbazole (Sigma-Aldrich). Color development was stopped by washing several times with water ...
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bioRxiv - Immunology 2020Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...
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bioRxiv - Genomics 2019Quote: ... were isolated from 10 ribs from 3 male goats and frozen down for subsequent stimulation with lipopolysaccharide (LPS) (Salmonella enterica serotype minnesota Re 595 (L9764; Sigma-Aldrich)) using the method described in (Clark et al. ...