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Citations for Millipore Sigma :
8801 - 8850 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 0.05% v/v Triton X-100) with 1/500 (v/v) plant-specific protease inhibitor cocktail and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). The protein concentration was quantified by the Bradford method using Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... 0.05% Triton X-100) supplemented with 1/500 (v/v) plant-specific protease inhibitor cocktail and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). 20 μL (bed volume ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: SDS washed and de-starched dry onion peels were weighed and added to a 2 mL screw – top microcentrifuge tube and 1 mL of 3 M HCl in methanol (Sigma, USA) was added to the walls and incubate for 16 h at 80°C in heating block in a fume hood as per De Ruiter et al ...
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bioRxiv - Plant Biology 2020Quote: ... and the 3 lines that germinated poorly after stratification showed >97% germination when sown on plates containing 10μM Gibberellin A4 (SIGMA ALDRICH, G7276).
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bioRxiv - Microbiology 2020Quote: ... Transverse sections (6 µm) were sectioned and mounted on silane-covered (TESPA, 3-aminopropyl-triethoxysilane, Sigma-Aldrich, St. Louis, Missouri, USA) glass slides ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were harvested by scraping the infected host-cell monolayers and passing host cells through 28g needles (3x) before removing host-debris through filtration (3 μm pore size, Millipore, TSTP04700). The flow-through was collected on ice and filters were rinsed with additional ice-cold PBS to maximize the yield of parasites ...
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bioRxiv - Microbiology 2021Quote: Metabolites were extracted from cell pellets through addition of 50 μl chloroform and vigorous vortexing, followed by 200 μl methanol/ultrapure water (3:1, containing 5 μM scyllo-inositol (Sigma I8132) as internal standard ...
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bioRxiv - Physiology 2021Quote: ... Sections were randomly selected for immunohistochemistry analysis using slides impregnated with 3% organosilane (Sigma Chemical Company®, St. Louis, MO, USA) in ethanol.
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bioRxiv - Microbiology 2021Quote: ... Virus supernatants were removed 7 h post-spin inoculation and cells were maintained in fresh growth medium for 48 h prior to selection in 3 μg/mL puromycin (Sigma-Aldrich). Stable cell lines were generated after 8 days of selection.
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bioRxiv - Microbiology 2021Quote: ... the cultures were harvested by centrifugation (20,000 × g for 3 min at RT) and the pellet was resuspended in a volume of 2× Laemmli buffer (Sigma-Aldrich) that adjusts for the total number of bacteria according to the OD600 of the initial cultures followed by 15 min incubation at 95 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Major peaks were desalted by RP-HPLC using an Ascentis C18 column (3 μm, 4.6mm ID x 15 cm, Sigma-Merck, Germany) using peak-based collection (slope) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... membranes were washed (3×10 min) with TBS-T and incubated with the corresponding secondary antibody conjugated with peroxidase (Sigma-Aldrich) for 1 h at rt ...
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bioRxiv - Microbiology 2021Quote: ... The infected host cells were incubated for 7 min at 37°C with DMEM containing 3 μM of calcium ionophore A23187 (C7522, Sigma-Aldrich) prior to fixation with 4% PFA ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated onto collagen-coated plates in Opti-MEM for 3 hours and subjected to respective treatment conditions of either glucose (D-(+)-Glucose (Sigma Aldrich) or erythropoietin (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... glutaraldehyde buffered with 3× PHEM (120 mM PIPES, 50 mM HEPES, 20 mM EGTA, 4 mM magnesium chloride, all from Sigma-Aldrich) (Montanaro et al ...
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bioRxiv - Molecular Biology 2021Quote: Exponentially growing Jurkat Tat KB001 or mdHIV clone 11 cells (3×107 cells/IP) were fixed with 1% formaldehyde (Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: The Nafion films on the IDAs mounted in the Teflon cell were loaded with various amounts of Ru(NH3)6 3+ by either exposing the films to a 10 mM solution of Ru(NH3)6Cl3 (Sigma Aldrich) in 0.2M Na2SO4 at time points from several minutes to several hours or by allowing Ru(NH3)6 3+ in the films to diffuse out into bulk electrolyte for several hours ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected at a density of 3 x 106 with equal amounts of heavy and light chain paired plasmids using polyethyleneimine (PEI, Sigma-Aldrich) at a ratio of 1:3 of total amount of plasmid to PEI ...
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bioRxiv - Cancer Biology 2020Quote: ... The effect of the anti-angiogenic compounds were measured using the 3-[4,5-dimethylthiazol- 2yl]-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma, Lyon, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were cleaned using stage-tips preparations using 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction.
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bioRxiv - Biophysics 2020Quote: ... were treated for 20 min at room temperature with 100 μl of Bind-Silane solution – a mixture of 3-(trimethoxysilyl)propylmethacrylate (7.15% by volume, Sigma-Aldrich, M6514) and acetic acid (7.15% by volume ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 3X with EGM-2 medium and re-suspended in PBS containing 3 mg/ml of fibrinogen (Sigma, #F8630) and 0.15 U/ml of aprotinin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Unroofed cells were incubated in PBS containing 3% w/v bovine serum albumin (Fisher Bioreagents, BP9703) and 0.1% v/v triton X-100 (Sigma-Aldrich, T9284) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... All progenies of genetic crosses were verified by diagnostic PCR using KOD XTREME Hot Start DNA Polymerase (71975-3, EMD Millipore) or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated for 24 hours with either 1 mM 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (Deferiprone/DFP, Sigma-Aldrich, 379409), or incubated in Earl’s balanced salt solution (EBSS ...
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bioRxiv - Cell Biology 2021Quote: ... validated lentiviral constructs (pLKO.1) expressing Mission shRNA targeting the following region 5’-GCTAGATTTACTAATGCCA-3’ in CPAP or scrambled shRNA were purchased from Sigma-Aldrich. For generation of stable cells ...
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bioRxiv - Cell Biology 2021Quote: ... Then, oocytes were transferred to a drop of chromosome spread solution (0.16% Triton-X-100, 3 mM DTT (Sigma-Aldrich, 43815), 0.64% paraformaldehyde in distilled water ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Cell Biology 2021Quote: ... the carboxylic acid groups on the Dynabeads® were activated by a 30-minute incubation with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC, cat. num. 39391, Sigma) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by blue native electrophoresis in 3-12% gradient gels (82, 83) using 10mg/ml Ferritin (404 and 880 kDa, Sigma F4503) and BSA (66 and 132kDa ...
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bioRxiv - Immunology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and antigen retrieved using proteinase K (Sigma-Aldrich, P6556) in Tris-EDTA pH8 buffer for 4 min at room temp ...
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bioRxiv - Molecular Biology 2022Quote: Human hPSCs cultured on MEFs were harvested using collagenase IV as big aggregates and settled 3 times in washing media (DMEM [Thermo Fisher Scientific], 5% Newborn Calf Serum [Sigma] ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was resuspended in lysis buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 5 mM imidazole, 3 mM βME, and protease inhibitor cocktail from Sigma-Aldrich: P8849), lysed by sonication ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded into 6-well plates so that they reached 50-60% confluency on the day of infection and transduced at most 3 consecutive days with the viral stock in the presence of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Biochemistry 2022Quote: ... The final proteins were concentrated in a prewashed Amicon Ultra-15 Centrifugal filter (molecular weight cut off 3 kDa; EMD Millipore), flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
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bioRxiv - Developmental Biology 2022Quote: ... RNAs immersed in Trizol were added to phase-lock heavy gel tubes (Brinkmann Instruments, INC., #955-15-404-5) and mixed with 100 uL of 1-bromo-3-chloropropane (BCP) (Sigma, #B9673), followed by room temperature incubation for 10 minutes ...