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Citations for Millipore Sigma :
8751 - 8800 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... the PDCD4 shRNA oligonucleotides (5‘-CTGGACAGGTGTATGATGTGG-3’) were synthesised and cloned into the MISSION® TRC2 pLKO.5-puro Empty Vector (SHC201, Sigma) by TsingKe Ltd ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... All animals were anesthetized prior to amputations using 0.1% Ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma-Aldrich, St Louis, MO, USA). Amputations were performed on the right forelimb of each animal at mid-zeugopod level ...
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bioRxiv - Immunology 2019Quote: ... larvae were kept under anesthesia in egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma-Aldrich, the Netherlands). The culture of zebrafish with mutations in immune genes was approved by the local animal welfare committee (DEC ...
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bioRxiv - Immunology 2019Quote: ... The microarray was then rinsed for 3 times with washing buffer and incubated with 200 μL of Horseradish peroxidase (HRP) conjugated Rabbit anti-Goat IgG (Sigma-Aldrich) diluted 1:20000 in Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The absorbance of the reaction mixture was measured at 500 nm and the total flavan-3-ol content was calculated from a calibration curve prepared using (+)-catechin (Sigma-Aldrich) and expressed as milligrams per gram of dried extract ...
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bioRxiv - Physiology 2021Quote: ... 2.5 CaCl2 and 10 glucose) to which was added 3 mg/mL collagenase I (Sigma Chemical Co., St Louis, MO, USA) and 1 mg/mL trypsin inhibitor (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... Single colonies were collected and used to inoculate a 3-mL YEB (yeast extract broth) starter containing 10 µg/mL cephalexin hydrate (Cp, Sigma-Aldrich). Starters were grown overnight at 28°C with 185-200 rpm shaking and then used to inoculate 500 mL of PSB (peptone sucrose broth ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Genetics 2021Quote: The CTCF protein was depleted in CTCF-AID mESCs by adding 500 μM of indole-3-acetic acid (IAA, a chemical analog of Auxin; Sigma-Aldrich) to the medium for 24 hrs ...
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bioRxiv - Microbiology 2020Quote: ... or mock nucleofected in the presence of one of the following inhibitors (auto= autophagy inhibitor, 3-methyladenine, authophagy inhibitor-M9281, Sigma Aldrich; apo= apoptosis inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Developmental Biology 2020Quote: ... The common bile duct was clamped at the duodenum and a 30G needle was used for perfusion of 3 mL Collagenase XI (1000 U/mL, Sigma-Aldrich) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2021Quote: ... larvae were anesthetized in E3 media without methylene blue supplemented with 0.16 mg/ml Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Genomics 2020Quote: Zebrafish embryos/larvae were euthanized by prolonged immersion in 200-300 mg/L tricaine (MS222; ethyl-3-aminobenzoate methanesulfonate, #E10521-10G, Sigma-Aldrich). Whenever necessary the chorion was removed ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated at 37 °C for 30 min to achieve maximum polymerization and then were mixed in a 1:1 volume ratio with pre-warmed PM buffer containing 3 mM MgCl2 and 2% Methylcellulose (MO512, Sigma-Aldrich). Final concentrations of protein ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1μm centrifugal filter (Millipore ...
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bioRxiv - Bioengineering 2021Quote: ... RBCs were suspended at a concentration of 104 cells/ml in the AS-3 media supplemented with 1 mg/ml of FITC-Dextran (Sigma-Aldrich). The solution containing the cells was introduced into one of the microfluidic devices described previously and imaged using a Leica DMI 6000B inverted phase contrast microscope (Leica Camera ...
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bioRxiv - Bioengineering 2021Quote: ... The primary antibody buffer was 3% horse serum and 5% DMSO dissolved in 1X PBS with 0.2% Tween-20 (Sigma Aldrich, P2287) and 10 μg/mL heparin (Sagent Pharmaceuticals ...
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bioRxiv - Bioengineering 2021Quote: ... and then seeded atop the Matrigel-coated surfaces at a density of either 1×105 cells/cm2 (96-well plates) or 3×105 cells/cm2 (hydrogels) in CMGF+ medium supplemented with 10 µM Y-27632 (EMD Millipore) for 24 h to form monolayers ...
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bioRxiv - Bioengineering 2020Quote: ... 15 μL droplets of cell-hydrogel mix were placed on the surface of elastomeric membranes that underwent a silanization process through a nitric acid and (3-Aminopropyl)triethoxysilane (Sigma-Aldrich) treatment ...
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bioRxiv - Bioengineering 2020Quote: ... P(VDF-TrFE) 75/25 weight % (Solvay Solexis) was dissolved in a 3:2 volume ratio of dimethylformamide (DMF)/acetone (Sigma-Aldrich) at a polymer-solvent concentration of 15-35% w/w ...
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Bioengineering 2020Quote: ... at 20-25×105 cells/dish and cultured in primary reprogramming medium (StemMACS iPS-Brew XF medium (iPS-Brew; Miltenyi Biotech) supplemented with 3 μM CHIR99021 (Sigma-Aldrich) and 10 μM SB431542 (Selleckchem ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Biochemistry 2020Quote: ... The instrument response function (IRF) of the system was measured using a dilute aqueous solution (3% w/w) of LUDOX AM colloidal silica (Sigma-Aldrich). The full-width half maxima (FWHM ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μl plasma from a single animal was diluted in 15 ml PBS and concentrated 30-fold using 3 kDa filter tubes (Millipore UFC900324) at 4,000 rpm for 1.5 h ...
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bioRxiv - Neuroscience 2020Quote: ... cell death caused by drugs were detected by double staining the cells with 5 μl Hoechst 33342 (23491-52-3, Biotech, China) and Propidium iodide (PI) (P4170, Sigma, USA) for 15 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... mechanically dissociated cortices from P1-3 Wistar rats were cultured in poly-D-lysine (PDL)-coated (10 μg/mL; Sigma P7886) tissue culture plates (diameter 10 cm ...
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bioRxiv - Neuroscience 2020Quote: ... Stages VI oocytes from Xenopus tropicalis anesthetized by 0.4% ethyl 3-aminobenzoate methanesulfonate (MS-222)(Sigma-Aldrich; St. Louis, Missouri, USA) were prepared by treatment with 0.1 mg/ml collagenase (Worthington ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2020Quote: ... flies were collected 2-5 days post eclosion and grown for another 3-5 days on 1mM all-trans retinal (R2500; Sigma-Aldrich) supplemented food in complete darkness before experimental testing was performed ...
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bioRxiv - Cell Biology 2021Quote: ... Microstructures were then generated using high-resolution chrome masks (JD phototools, UK) and 365nm UV exposure (UV KUB 3, Kloe, France) followed by PGMEA (Sigma-Aldrich) development ...
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bioRxiv - Cancer Biology 2020Quote: ... protein was extracted using RIPA buffer with added cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma). For 2D cell culture ...
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bioRxiv - Cancer Biology 2019Quote: HBP inhibition in vivo was achieved by treatment of tumor bearing mice with 3 doses (10 mg/kg) of DON (6-Diazo-5-oxo-L-norleucine, Sigma-Aldrich) every 3 days ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on glass coverslips and bone slices for 3 days were fixed for 10 min in a 3.7% (wt/vol) paraformaldehyde (Sigma Aldrich 158127) solution containing 0.25% glutaraldehyde (Electron Microscopy Sciences 16220 ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed in 60% isopropanol and incubated for 30 minutes at 37°C in a freshly filtered solution of 3 mg/ml oil red O (O0625, Sigma Aldrich) in 60% isopropanol ...
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bioRxiv - Plant Biology 2021Quote: ... The tip was broken to achieve a 2-3 µm diameter and was front-filled with potassium ionophore I (Sigma, Switzerland), a liquid ion exchanger to achieve high resistance (4–6 GΩ) ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... DLD1-wildtype and DLD1-NXF1-AID cells were pretreated for 0-8 hrs with 0.5 mM synthetic auxin (3-indoleacetic acid, Sigma, in ethanol), prior to addition of 250 nM NVP-2 ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in urea buffer (50mM Tris HCl pH 7.5, 5mM EDTA, 6M Urea, 1% SDS, 1mM PMSF, 1:100 Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich P0044]) and cells were lysed by bead beating ...
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bioRxiv - Microbiology 2020Quote: ... The wells were further blocked with 15 µl of 10 µM biotin in 3% skim milk in PBST (Sigma, cat# 1.15363.0500) for ten min at R.T ...