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Citations for Millipore Sigma :
8951 - 9000 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the thiol groups of the above extracted protein samples from the culture supernatant of 3 groups were alkylated with 50 mM iodoacetamide (cat No. I6125, Sigma, USA) (30 min in dark at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The depleted serum was concentrated and the buffer was exchanged to 100 mM TEAB using Amicon 3 K concentrators (cat No. UFC500396, Millipore, USA). The procedures for serum protein denaturation ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione) was purchased from Sigma-Aldrich (St. Louis, MO). 8MM-IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione ...
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bioRxiv - Microbiology 2019Quote: ... berghei ANKA 2.34 infected blood containing asexual parasite and gametocyte stages with a parasitaemia of 5-6% and gametocytaemia of 2-3% was mixed with 150 μL of PBS containing either α-Pbc43 or the isotopic monoclonal UPC10 (negative control) (Sigma) antibodies to yield final antibody concentrations of 50 ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: Cells (passage 3) were suspended in solutions of GelMA (5%, containing 1% (w/v) of the photoinitiator (PI, Irgacure 2959, Sigma-Aldrich)) ...
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bioRxiv - Plant Biology 2019Quote: ... in the presence of 1 % v/v proteinase inhibitor mixture (Serva) and 0.5 % of each phosphatase inhibitor cocktails 2 & 3 (Sigma-Aldrich, Germany) in a Dounce Homogenizer ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue was washed 3 times with RPMI and then resuspended in a solution of 10 % Dispase (Cornig) 30 μg/ml DNAse I (Sigma-Aldrich) in RPMI (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... CAD and Neuro-2a cells were grown on Millicell Hanging Cell Culture Inserts with a pore size of 3 μm (Millipore #MCSP06H48), adapting experimental pipelines described earlier (Ludwik et al. ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Neuroscience 2021Quote: ... Then the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich). Images were acquired using a Zeiss 780 inverted confocal microscope ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μL of the overnight cultures were diluted into 3 mL of LB containing 25 μg/mL chloramphenicol and 1 mM IPTG (Sigma-Aldrich) and incubated at 37°C with shaking until early stationary phase (OD600 0.7-1.1).
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bioRxiv - Cell Biology 2021Quote: β-Estradiol induction of the pRPS5A::XVE:GFP-μHD line was done by transferring 3-day-old seedlings to medium containing β-Estradiol (Sigma-Aldrich) or solvent (DMSO ...
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bioRxiv - Physiology 2021Quote: ... Samples were then immersed in 5% (v/v) H2O2 in methanol for up to 3 hours (Sigma-Aldrich Cat No: H1009) for bleaching ...
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bioRxiv - Physiology 2021Quote: ... Five μL of each dilution were introduced into the AscentisSi column (10 cm × 1 mm, 3 μm, Sigma-Aldrich, Darmstadt, Germany) with a flow rate of 50 μL min−1 ...
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bioRxiv - Neuroscience 2020Quote: ... 3-5 kDa fluorescein isothiocyanate-dextran (FITC-dextran) and 65-85 kDa tetramethylrhodamine isothiocyanate (TRITC-dextran) were obtained from Sigma-Aldrich. Transwell permeable polyester membranes (400 nm pore ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked with 3% BSA/TBST for 30 min and incubated with rabbit anti-Sox9 polyclonal antibody (1:500, EMD-Millipore, AB5535), rat anti-endomucin (Emcn ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Cell Biology 2019Quote: ... RPMI 1640 complemented with indicated inhibitors was replaced daily for 3 days before overnight fixation with formalin at 4°C (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... cells were removed and plates were incubated with biotinylated detection antibodies and Streptavidin-HRP conjugate following the manufactures introductions using a 1 in 100 dilution for the streptavidin-HRP conjugate with 3-Amino-9-ethyl-carbazole (AEC; Sigma-Aldrich) dissolved in N,N-dimethylformamide (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: SH-SY5Y and HEK293 cells were treated with SB202190 (20 μM) for 3 h in the presence or absence of 100 nM Mito-TEMPO (Sigma-Aldrich) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Depletion of endogenous Spindly was achieved through reverse transfection with 50 nM Spindly siRNA (5′-GAAAGGGUCUCAAACUGAA-3′ obtained from Sigma-Aldrich) for 48 hours with RNAiMAX (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were combined and concentrated approximately 3-fold using a 30-kDa cut-off Amicon centrifugal filter unit (Millipore, Massachusetts). Approximately 5mL of concentrated protein was subjected to size-exclusion chromatography using the Äkta Pure system and a superdex 200 increase 10/300 gl pre-equilibrated with running buffer (50mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were isolated by centrifugation (12,300 x g, 3 minutes) and resuspended in 200 µL phosphate-buffered saline (PBS; Sigma-Aldrich, USA). Resuspended cells (200 µL ...
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bioRxiv - Synthetic Biology 2021Quote: ... Carboxyl groups on the photo-activated regions were activated with 50 µL/well of 0.05 g/mL N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 03450) and N-Hydrosuccinimide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen tissue was transferred to a pre-chilled 3 ml Kimble dounce homogenizer filled with 4ml Nuclei Lysis Buffer (Sigma, NUC101), supplemented with RNAase inhibitors (10mg/ml ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Pathology 2021Quote: ... The tissue was blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... Large aggregates were removed by filtering through a 100-kDa filter followed by concentrating the solution in a 3-kDa filter (Millipore Sigma). To create fibrils ...
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bioRxiv - Biochemistry 2021Quote: ... Any large aggregates were removed by filtering the solution through a 100-kDa filter and then concentrating the solution using a 3-kDa filter (Millipore Sigma). Samples were diluted to 70 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted, and re-suspended in lysis buffer (1 mM CaCl2, 3 mM MgCl, 1mM EDTA, 1% Triton X-100 (Sigma-Aldrich), 10 mM TrisHCl ...
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bioRxiv - Developmental Biology 2021Quote: ... stage embryos were isolated from oviducts in M2 media (pre-warmed at 37°C for at least 2-3 hours) and thereafter cultured in KSOM (Sigma-Aldrich, EmbryoMax KSOM Mouse Embryo Media ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000073737 5’-CCGGCGCGTTATCAACTGGATCCAACTCGAGTTGGATCCAGTTGATAACGCGTTTTTG-3’ designed and cloned into the lentiviral pLKO.1 puromycin resistant vector Mission shRNA lentiviral Transduction particle (Sigma Aldrich). Control Caco2 clones (shNT ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with 500 µL PBS and re-suspended in 500 µL of PBS containing ∼3 mg/mL Biotin-NHS (Sigma B1022). Cells were incubated for 30 min on a rotating wheel at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... injected embryos were manually dechorionated and anesthetized by incubation with 0.14 mg/ml Tricaine (Ethyl 3-aminobenzoate methanesulfonate; A5040, Sigma-Aldrich, USA).
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Evolutionary Biology 2021Quote: An overnight culture of Bacillus thuringiensis Berliner (ATCC 55177) was prepared from a glycerol stock and grown at 30°C in Nutrient Broth #3 (NB, Sigma Aldrich). An aliquot of overnight culture was brought to an OD of 0.5 in fresh NB ...
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bioRxiv - Molecular Biology 2021Quote: ... n = 3 for each group) were intraperitoneally injected with lipopolysaccharide (LPS; Escherichia coli 0111:B4, Sigma-Aldrich, St Louis, MO, USA), polyinosinic:polycytidylic acid [poly(I:C) ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 200 μL lysozyme from a 3 mg/mL stock solution and 10 μL lysostaphin from a 10 mg/mL stock solution (Sigma-Aldrich). Cells were incubated at 37°C for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... The exudate metabolites were separated on a SeQuant ZIC-pHILIC HPLC column (2.1 × 100 mm, 3 μm) (Milllipore Sigma, Burlington, MA) using acetonitrile with 0.1% formic acid as solvent B and water with 0.1% formic acid as solvent A at a flow rate of 400 μL min−1 and the following linear gradient extending over 14 min ...
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bioRxiv - Microbiology 2021Quote: ... was from Thermo Fisher Scientific (Massachusetts, USA). 3-MA (Cat no. M9281) and Anti-HA antibody (Cat no. A190-101) was from Sigma (Missouri, USA). Nontargeting siRNA (Cat no ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... followed by co-incubation with an excess of HeLa cells (recipient) for 3 h in the presence of 50 μg/ml cycloheximide (Sigma, # C104450), to prevent further protein synthesis in the heterokaryons ...
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bioRxiv - Microbiology 2021Quote: ... Allicin was synthesized by oxidation of 3-[(Prop-2-en-1-yl)disulfanyl]prop-1-ene (diallyl disulfide, Sigma-Aldrich, Germany) with peracetic-acid (glacial acetic acid/H2O2 ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...