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Citations for Millipore Sigma :
8651 - 8700 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... DNA was injected into the fourth ventricle at a final concentration of 1-3 µg/µl in addition to trace amounts of fast-green dye (Sigma). Three 50ms square waveform electrical pulses at 5V (E2 ...
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bioRxiv - Cancer Biology 2024Quote: ... We washed the slides for 5 min in 2× SSC/0.1% IGEPAL at RT followed by a 3-minutes wash at 60 °C in 0.4× SSC/0.3% IGEPAL (Sigma-Aldrich Inc.), and an additional 3-minutes wash in 2× SSC/0.1% IGEPAL at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
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bioRxiv - Neuroscience 2024Quote: ... Anatomical images were acquired from fish anesthetized with 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich). To activate TRPV1-expressing Purkinje cells ...
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bioRxiv - Cell Biology 2024Quote: ... Moreover, a non-targeting control siRNA (NT siRNA, or siCON) with the sequence 5’-CGUACUGCUUGCGAUACGGUU-3’ was used (Sigma-Aldrich). Cells were transfected with 22 pmol of siRNA oligonucleotides/well using Lipofectamine RNAiMAX (ThermoFisher Scientifc ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... each coverslip was washed with PBS 3 times each for 5 minutes and nuclei were stained with Hoechst (Merck Sigma, Burlington ...
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bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... spores were sown directly on plates supplemented with Indole-3-acetic acid (IAA; Alfa aeser) or 1-Naphthaleneacetic Acid (NAA, Sigma). Size measurements were done when plants reached sexual maturity (±6/7 days) ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted from the central body cells of 3-week-old WT and Col5a1+/− menisci by homogenizing freshly dissected tissues in TRI-reagent (T9424, Sigma) and phase-separated in 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed x3 in 0.24M phosphate buffer solution (PB)(Table 3) and left overnight in 0.12PB with 15% Sucrose (Sigma, 84100-1KG) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then collected by centrifugation and washed with 3% FBS in PBS followed by PI (Millipore Sigma, catalog P4170) staining (25 μg/mL PI ...
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bioRxiv - Biochemistry 2024Quote: ... the column was washed using 50 mL of 10 mM Tris buffer (pH 7.4) containing 100 mM NaCl and 3 % Empigen BB (Sigma-Aldrich) to replace urea and SDS with Empigen ...
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bioRxiv - Biochemistry 2024Quote: ... the enzyme was incubated with 100 μM KCN or 500 μM 3-amino-1,2,4-triazole (ATZ, Sigma, Cat# A-8056) for 30 minutes at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Neuroscience 2024Quote: ... and the samples were washed 3 times for 5 min in PBS and counter-stained with 1:1000 DAPI (D9542, Sigma) for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 10 min) and resuspended in 3-5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5xCellLytic B [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2024Quote: ... bacterial cultures in the exponential phase were collected and then thoroughly mixed with 3 wt% (w/v) of sodium alginate (Sigma) solution to a final concentration of 2.5% alginate ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Immunology 2024Quote: ... Non-specific binding sites were blocked using 300 µl/well of 3% (w/v) skim milk powder (Sigma-Aldrich, USA) in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: Cardiac cells were fixed with cold 4% paraformaldehyde (PFA) solution for 20 min at RT and then washed three times in PBS and once in 3% BSA (Sigma) in PBS for 5 min ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultivated under humidified conditions with 5% CO2 at 37°C and passaged every 3-4 days using 0.05% trypsin/EDTA (Sigma-Aldrich). Cells were harvested in ice-cold PBS buffer using a cell scraper ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 × 107 CFU were added to a flow cytometry tube containing 1 mL buffer with 10 μM DiOC2(3) (Sigma) and incubated in the dark for 30 min at 30 0C ...
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bioRxiv - Immunology 2024Quote: ... infected cells at different time-points were washed 3 times in 1xPBS and lysed with 0.1% Triton X-100 (#X100 Sigma-Aldrich) in H2O ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated for 3 hours with 50 ng/ml phorbol myristic acid (PMA; Sigma-Aldrich, St Louis, MO, USA), 1 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in 0.01% PBS-Tx (1xPBS with 0.01% Triton X-100 (Sigma-Aldrich, Cat#X100)) for 10 mins at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were suspended and incubated for 3 min in 350 µl PBS mixed with 150 µl 0.4% trypan blue solution (Millipore Sigma). For determining viability ...
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bioRxiv - Neuroscience 2024Quote: ... Two length-matched siblings were anesthetized in 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich) simultaneously and mounted in 2% low-melting temperature agar ...
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bioRxiv - Immunology 2024Quote: ... approximately 30 g of spleen tissue was placed in R10 supplemented with 10U/mL DNase I and 3 mg/ml collagenase D (Sigma), the capsule was removed ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.3∼7.4) and then vacuumed infiltrated using a vacuum drying cabinet (Yiheng, Shanghai, China) with a vacuum pump (Millipore, MA, USA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 2015) e cleaned using stage-tips preparations with 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction following the manufacturers instructions.
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bioRxiv - Plant Biology 2019Quote: ... A ratio of 1:2 and 1:3 (v/v) polyvinylpolypyrrolidone (PVPP, ~110 µm particle size, Sigma-Aldrich, Saint Louis, USA) to ground tissue was used ...
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bioRxiv - Synthetic Biology 2021Quote: ... Extracts were then clarified at 4’000 × g for 10 min and concentrated to ~4 mg/mL total protein concentration using 3 kDa molecular weight cutoff (MWCO) Centriprep concentrators (EMD Millipore) to account for dilution during preparation ...
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bioRxiv - Biochemistry 2020Quote: Gene expression of endogenous CENP-C was inhibited using a single siRNA (target sequence: 5’-GGAUCAUCUCAGAAUAGAA-3’ obtained from Sigma-Aldrich), which targets the coding region of CENP-C mRNA ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using a commercial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) viability assay (Sigma Aldrich, Rehovot, Israel). That assay was used to determine how highly 4T1 proliferation was stimulated by Norepinephrine (NE ...
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bioRxiv - Cell Biology 2020Quote: ... cells were single-cell sorted into 96-well low-bind PCR-plates [Eppendorf] containing 3 μl of lysis buffer (0.5 units/μl RNase inhibitor [Takara], 0.15% Triton X-100 [Sigma], 0.5 mM (each) dNTP [Thermo Scientific] ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then washed 3 times for 5 min in 0.3% TritonX (Simga; T8787) in PBS and blocked for 1 hr at RT in 5% normal donkey serum (NDS, Millipore; S30), 1% BSA 0.3% TritonX in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown for 3 days on coverslips until they reached confluence and then 50 ug/ml L-ascorbic acid-2-phosphate (Sigma Aldrich) was added to cultures ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were blocked for at least 2.5 hours at room temperature followed by the addition of poly-clonal rabbit anti-histone H4 (citrulline 3) antibody (EMD Millipore) used at 1:100 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: NHP heart slices seeded with HVPs/CMs after RFA injury were loaded with 3 μM Fluo-4-AM in CCM (without phenol red) supplemented with 0.75% Kolliphor EL (Sigma Aldrich, USA) by incubation at 37°C for 60 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Developmental Biology 2021Quote: ... Tadpoles were then washed 3×10 minutes in PBT followed by a 10-minute incubation in 1:2000 DAPI (Sigma D9542) before being washed with 1xPBS for 10 more minutes ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Genetics 2021Quote: ... was generated by cloning the 3HA epitope CDS fused in-frame with the 3′ end of the RNAi resistant dCNBP CDS into the UAS-attB vector (Genewiz, SIGMA-ALDRICH). The plasmids for inducible expression of the human CNBP counterpart was generated by cloning the FLAG epitope CDS fused in-frame with the 3′ end of the hCNBP CDS (CNBP-201 splice variant ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then incubated in LB for 3 hours at 37 °C and plated in LB agar with Kanamycin (Sigma-Aldrich) for 24 hours at 37°C ...