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Citations for Millipore Sigma :
8451 - 8500 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... a 188 bp cDNA fragment of MtABCG40 5’UTR or a 200 bp cDNA fragment of MtLOG3 3’UTR were amplified with KOD Hot Start DNA Polymerase (Novagen) and cloned into pDONR/Zeo vector (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with U6-gDNA (5’-AAAGACGTCCCTAACAAGT-3’; clone ID es: HSPD0000063884): CMV-eCas9-2a-tGFP (Sigma-Aldrich). 48h after transfection ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the hairpin were selected with 1 μg/ml puromycin and also sorted by GFP flow cytometry after 3 days of 100 ng/ml doxycycline (Sigma) treatment.
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted ventrally (ventral side down) on to a custom molded 3 cm plate using 0.8% low-melt agarose (Sigma- Aldrich). Plates were perfused with E3 media (5mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... The remaining iron nitrate salt was allowed to cool then quantified using a colorimetric iron assay kit with 3 technical replicates for each cell type (MAK025, Sigma). The total iron from 3 technical replicates was averaged then divided by the number of cells to obtain calculated pg Fe/cell.
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bioRxiv - Plant Biology 2024Quote: ... 2.5 cm sections were dissected from excised leaves (cut 3 cm from the leaf tip) and placed on 12 cm2 0.5% agar (type M; Sigma-Aldrich) Petri plates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated for 30 minutes with 3 µmol/L 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma-Aldrich, D225). After that ...
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bioRxiv - Immunology 2024Quote: ... AHR was assessed by administering incremental doses of nebulizing methacholine (0, 1, 3, 9, 27, 81 mg/mL; Sigma-Aldrich) and measuring resistance every 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting ribosome solution (∼1 mL) was further concentrated using 0.5 mL Amicon Ultra Filter unit 3 KDa MWCO (Sigma-Aldrich) by centrifugation at 14,000 RCF and 4°C ...
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bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to confocal imaging except where noted ...
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bioRxiv - Neuroscience 2024Quote: ... homogenized in cytosol lysis buffer (10 mM Hepes, 40 mM KCl, 3 mM MgCl2, 5% glycerol, 0.2% NP40, 1 mM DTT, cOmplete protease inhibitors, Sigma-Aldrich) and incubated for 30 min at 4°C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in 0.4 µm or 3.0 µm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3– 5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell insert for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were housed singly for 2–3 days after the procedure and were subsequently head-fixed to a custom mount with eicosane (Sigma) and imaged on a Movable Objective Microscope with resonant scanners (MOM ...
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bioRxiv - Cell Biology 2024Quote: ... stage were collected 48 hours after injection and released into M2 medium containing 200 µM 3-isobutyl-1-methylxanthine (IBMX, Sigma) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Genomics 2023Quote: ... with 10% charcoal-stripped FBS (#100-119, GemBio) for 3 days and then were stimulated with 10nM dihydrotestosterone (DHT) (A8380, Sigma) for 0.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... All antibody incubations were done in block solution containing 5-10% Normal Donkey serum and 3% Bovine Serum Albumin (BSA, Sigma). After early initial staining ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Cell Biology 2024Quote: Worms of the indicated genotype were selected as L4s or young adults (24h post L4 stage) and picked onto NGM plates with and without 1 mM indole-3-acetic acid (auxin, Sigma). Exposure to auxin was carried out for the indicated times ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm, Sigma). Coverslips were mounted on glass slides and visualized using CLSM with a 488 nm laser.
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bioRxiv - Microbiology 2024Quote: ... Cultures were induced by addition of DNA-damaging antibiotics to a final concentration of 3 μg/ml: mitomycin C (Sigma), norfloxacin (Sigma ...
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bioRxiv - Plant Biology 2024Quote: Auxin treatments were performed by immersing dissected SAM in 1 or 2 mM Indole-3-acetic acid (IAA) solution (Sigma) for five hours as described (Galvan-Ampudia et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed again with PBS to remove residual detergent and then blocked in 3% bovine serum albumin (BSA) (Sigma) in PBS for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... or M9 minimal media supplemented with fructose or glucose (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl (Sigma–Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes followed by 3 washes in 40mM Tris buffer (pH=7.2) and then 1:20 Giemsa solution (Sigma-Aldrich). The blood and bone marrow were then imaged on a Zeiss Axio Imager.A2 and Zen Blue software (version 3.1).
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bioRxiv - Cell Biology 2023Quote: ... blots were again washed 3 times (5 min per wash in Tween-20 saline) before addition of ECL (Millipore, MA) for chemiluminescent detection on a BioRad Image Analyzer using Image Lab to capture and quantify signal intensity ...
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bioRxiv - Microbiology 2023Quote: ... All intracellular metabolite samples were resuspended in 750μL deuterium oxide containing 1mM 3-(Trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) (178837, Sigma Aldrich) and 50mM NaIO4 (769517 ...
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bioRxiv - Biophysics 2024Quote: ... Fluorecent probe Atto488-DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto-488) was purchased from Sigma-Aldrich (international). Sucrose ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed again 3 x 5min with ddH2O at room temperature and incubated in thiocarbohydrazide (TCH, 0.22mm Millipore filtered) solution for 20 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Developmental Biology 2022Quote: ... The wells were washed with ddH2O for 3 times and incubated with 100ul/well of 10μg/ml fibronectin bovine plasma (Sigma-Aldrich) in 0.1% gelatin for 2 hours on an orbital shaker at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Microbiology 2023Quote: ... they were washed 3 times with 1M sorbitol and resuspended in RPMI 1640 broth containing protease inhibitor cocktail (Sigma-Aldrich) to final concentration of 3×108 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were subsequently washed 3 × 5 min in 1x TBS/ 0.1% Tween-20 and developed using Immobilon Western HRP Substrate (Millipore, WBKLS0S00) and imaged using the Azure C300 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... To the bead suspension was added 0.5 mL of 750 mM of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in water (Sigma-Aldrich) and the mixture was incubated for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... Splenocytes were incubated for 1 hour at 37°C in 96-well plates in RPM11640 medium (3 ml) (Sigma-Aldrich) with or without EPO (35U/well ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed in TBS (3 washes for 5 min each) and then treated with 0.25% H2O2 (Cat#216763, Sigma-Aldrich), in TBS for 3 min ...