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Citations for Millipore Sigma :
8401 - 8450 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or TAP-HA-YTM1 RPL3-Flag fusions under control of the respective endogenous promoters were grown in YPD or SDC-Leu medium and as indicated in the figures incubated in the presence of 0.5 mM auxin (3-indoleacetic acid, Sigma-Aldrich) for 120 min prior to harvesting the cells ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The dissociated cells were washed in PBS 3 times and treated with blood cell lysate buffer (Sigma-Aldrich, Cat# R7757).
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cell Biology 2022Quote: ... or 100 pmol (third and fourth replicate) of the internal standard (22E)-Stigmasta-5,7,22-trien-3-beta-ol (Sigma-Aldrich, R202967) using 100 μl acetic anhydride/chloroform (1:12 v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... approximately 1 hour after the start of the light period the female now was removed from the tank and anesthetized briefly in another tank with egg water containing 0.02% buffered ethyl 3-aminobenzoate methane sulfonate (Tricaine, Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed (3 × 5 min. in PBS at RT) before staining with DAPI (1:1000; Sigma Aldrich, D9542) and AlexaFluor-488 Phalloidin (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet was resuspended in 50 µL cold lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal CA-630, Sigma-Aldrich, I8896) and incubated for 2 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were again washed for 3 × 10 minutes in PBS followed by staining in DAPI solution for 20 minutes (Sigma). Coverslips were mounted on slides in ProLong™ Gold antifade ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
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bioRxiv - Genomics 2022Quote: ... 75μl of a mix of the 16S probe (5’-GGATTAGATACCCBDGTAGTCGAGATNBAAAAAAAAAAAAAAAAAAAA-3’, IDT, USA) at 0.6μM concentration with 4X Saline Sodium Citrate (SSC) (SSC, Sigma-Aldrich, USA) and 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Microbiology 2022Quote: ... during the exponential growth phase after adding 100 μM of the PSII blocker 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma-Aldrich, USA).
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bioRxiv - Physiology 2022Quote: Pancreata were injected through the portal vein with 3-5 mL cold Hanks buffer containing 0.95 mg/mL collagenase V (Sigma, USA). The collected pancreata were incubated at 37°C for 7 min ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then washed in 1X PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Microbiology 2022Quote: ... 5 A260 units of the cleared cell lysates were incubated either for 30 min at RT either with 3 µg of micrococcal nuclease (Sigma), or 300 units of RNase T1 (Fermentas ...
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bioRxiv - Molecular Biology 2022Quote: ... microfluidic channels used for imaging were also supplemented with the oxygen scavenging system: 3 u/ml pyranose oxidase (Sigma-Aldrich), 90 units/ml catalase (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: Fly heads from 5 flies (3 samples collected per genotype) were homogenized using a motorized pestle and 50 μL RIPA buffer (Sigma) containing protease and phosphatase inhibitors (cOmplete and PhosStop ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM NaCl) using a 3 kDa (or 10 kDa) molecular weight cut-off (MWCO) Amicon Ultra Centrifugal Filter Unit (Merck Millipore), and used directly or aliquoted ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures were grown in a mixture of 2:3 ratio of Todd-Hewitt media with 0.5% yeast extract (Sigma-Aldrich), and Brain-Heart Infusion media (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... Fungal transformants were plated on GG10 medium (Geib and Brock, 2017; Geib et al., 2016) containing 3 μg/ml thiamine (Sigma) (GG10THI ...
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bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... were diluted in PBS together with 3 μg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma) and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Auxin-induced degradation of Ring1b in AID::Ring1b;FUCCI was carried by adding water-dissolved auxin (IAA, Indole-3-acetic acid sodium salt, Sigma) to cell media to a final concentration of 500 µM as described previously 46 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Eluted protein samples were combined in one tube and desalinated using an ultrafiltration tube (Amicon Ultra 3 kDa molecular weight cut-off, Merck/Millipore) with Buffer C (25 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-HA gels were washed 3 times with 20 ml TBS-T and then incubated with peroxidase-conjugated secondary antibody (1:10,000; Sigma A0545) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418; Sigma). The culture was incubated with shaking (120 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... ∼7-8 million hypothalamic neurons segregated by sex were cultured during 3 DIV and then fixed with fixation buffer containing 1.1% formaldehyde (Sigma-Aldrich). Cross-linked cells were scraped ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... Proliferative capability of BMECs was tested by 3-[4,5-dimethylthiazyol-2-yl] -2,5-diphenyltetrazolium bromide (MTT, 5mg/ml, Sigma, cat# CT01) assay ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Neuroscience 2023Quote: ... samples were rinsed 5 × 3 min with double distilled water (ddH2O) and subsequently exposed to 1% aqueous thiocarbohydrazide (TCH) (#88535 Sigma) solution for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Auxin treatment was performed by transferring worms to bacteria-seeded NGM plates containing 1 mM auxin (indole-3-acetic acid, Sigma). The preparation of auxin stock solution (400 mM in ethanol ...
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bioRxiv - Neuroscience 2022Quote: ... and then washed with PB (3 × 5 min; RT) and finally mounted on gelatinised slides and coverslipped with Mowiol DABCO (Sigma). For brightfield microscopy ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Molecular Biology 2022Quote: ... blood was collected via cardiac puncture from anesthetized mice (100 mg/kg ketamine and 3 mg/kg xylazine, Sigma-Aldrich). Whole blood aliquots were stored in liquid nitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and a 0.2 nM αV imager strand buffer solution (5-GGAGGA-3’-Atto643, Eurofins) were made using 1× PCA (Sigma-Aldrich), 1× PCD (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... Doxycycline-inducible expression was tested in each cell line using 3 different concentrations (0.1-1 μg/mL) of doxycycline hyclate (Sigma-Aldrich) and a no-doxycycline control to evaluate Orf3a expression leak ...
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bioRxiv - Neuroscience 2022Quote: Tissues or cells were homogenized in Pierce IP Lysis buffer supplemented with protease inhibitor cocktail 3 (Merck Millipore, Darmstadt, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... cells were synchronised in early S phase by two consecutive rounds of treatment with 2 mM thymidine and 3 ug/ml (Sigma) aphidicolin ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292; Sigma Aldrich] and 22,5mg/ml trypsin in Thyrode’s solution pH 7.4) ...
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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bioRxiv - Cell Biology 2024Quote: ... The flat mount was then rinsed with HBSS and incubated in blocking buffer made with HBSS with 5% bovine serum albumin (Catalog #BP9703-100) (W/V) and 3% Triton X-100 (Sigma) (V/V ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...