Labshake search
Citations for Millipore Sigma :
7751 - 7800 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Then, compressed air (DAN oxygen kit, Divers Alert Network, USA) and 25 µM of sodium sulfide (Na2S*9H2O; Sigma-Aldrich, St. Louis, MS, USA) were injected into the oxic and anoxic incubations ...
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bioRxiv - Immunology 2019Quote: ... Serum and lung immune factors were analyzed by multiplex technology (performed by Eve Technologies, Calgary, AB, Canada) using a 31 cytokine/chemokine/growth factor kit (Millipore, St Charles, MO, USA). The assay was run on a Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Hydrogen peroxide from 40 hippocampus samples of mice of both strains (n = 5 per group) was measured as an indicator of oxidative stress and it was quantified using the Hydrogen Peroxide Assay Kit (Sigma-Aldrich, St. Louis, MI) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Genomic DNAs were prepared from selected isolates showing different cell and colony morphologies using GenElute™ Bacterial Genomic DNA Kit (Sigma Aldridge, Gillingham, UK). Identification to presumptive species level was carried out by performing PCR amplification of 16S rRNA gene sequences using primers TPU1 and RTU8 (73 ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Pathology 2019Quote: ... and TGF-β1 in the samples was measured using magnetic bead-based multiple immunoassays (MILLIPLEX® MAP kit, Human High Sensitivity T Cell Magnetic Bead Panel: EMD Millipore Corporation, Germany). Each assay included two calibration curves for each of the proteins to be measured (calibration ranges ...
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bioRxiv - Genomics 2021Quote: ... genotyping for the Ile148Met variant associated with hepatic steatosis (rs738409) was performed on genomic DNA extracted from the blood buffy coat using the GenElute Blood Genomic DNA kit (Sigma, St. Louis, MO, USA). rs738409 was genotyped using validated primers and TaqMan probes (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... Half of the sample was proceeded for genomic DNA isolation using GenElute plant genomic DNA miniprep kit (Sigma-Aldrich Co., St. Louis, MO, USA) according to the manufacturer’s instructions for PacBio and Illumina platforms sequencing ...
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bioRxiv - Immunology 2021Quote: ... Ten μl were removed from each tube to measure C8 or longer free fatty acids (i.e., palmitic acid released from PGPC or POVPC) (Sigma free fatty acid kit MAK044) and the remaining samples were stored at -80℃ prior to use or analysis.
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 females from each biological replicate were flash frozen in liquid nitrogen and total RNA was isolated using the GenElute Mammalian Genomic RNA miniprep kit (Sigma Aldrich, CAT# RTN350-1KT), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: WT BBS and BBS Mut cells subjected to normoxic or hypoxic treatment were lysed using assay buffer provided in the Phosphofructokinase (PFK) Activity Colorimetric Assay Kit (Sigma, MAK093, lot no. 6E31K07760). An equal quantity of protein lysates was used to determine the total PFK activity as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the nuclear fraction was prepared from whole plants using the ‘Semi-pure Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich, St. Louis, MO). Genomic DNA was isolated from the nucleus or whole plant using a DNeasy Plant mini kit (Qiagen ...
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bioRxiv - Physiology 2023Quote: ... and L-citrulline were quantified with HPLC-MS as previously described.49 Arginase activity was assessed with an arginase activity assay kit (Sigma Aldrich, St. Louis, MO) following manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
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bioRxiv - Physiology 2022Quote: ... triglyceride and glycerol (Triglyceride-SL Assay, Cat# 236-60, Sekisui Diagnostics, PEI, Canada) and pyruvate (Pyruvate Assay Kit, Cat#MAK071, Sigma-Aldrich, Oakville, ON, Canada) were measured using colorimetric assays according to the manufacturers’ directions ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from endothelial cells with the TRIzol reagent and Poly(A)+ RNA was purified using the GenEl-ute mRNA Miniprep Kit (Sigma, Louis, MO, USA; MRN10). The m6A concentration was assessed using the m6A RNA Methylation Kit (Abcam ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Biophysics 2023Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St. Louis, MO, 203351) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluency ...
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bioRxiv - Immunology 2023Quote: L-lactate level was assessed in plasma using colorimetric assays according to the manufacturer’s protocol with slight modifications (Lactate Assay Kit, Sigma-Aldrich, Missouri, USA, # MAK064-1KT). 25 μl of lactate standards or plasma samples were mixed with an equal volume of Master Reaction Mix (consists of ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from leaf samples (three biological replicates for each genotype) and using Spectrum Plant Total RNA Kit (Sigma Aldrich, Saint Luis, USA) following instruction ...
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bioRxiv - Immunology 2023Quote: ... Blood Urea Nitrogen (BUN) was also determined in the same serum samples using a urea colorimetric assay kit (#MAK006, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: ... bile salts were supplied from bovine bile and measured using a commercial kit according to the supplier’s protocol (Sigma-Aldrich, cat. No. MAK 309). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue lysis was performed in Roche cOmplete Lysis-M reagents (lysis buffer and protease inhibitor cocktail contained in the kit, Millipore Sigma cat. Nr. 4719956001). Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: LDH activity was quantified in the serum samples using a lactate dehydrogenase activity assay kit (catalog number MAK066, Sigma-Aldrich, St. Louis, MO, USA). The assays were performed according to the manufacturer’s instructions using serum samples diluted 1:10 in 1x Dulbecco’s phosphate buffered saline (1× DPBS ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 16 were subjected to in-situ Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) using the ApopTag Plus Fluorescein Kit (EMD Millipore, Burlington, MA, USA), following previously established protocols (Maddala et al. ...
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bioRxiv - Immunology 2021Quote: ... At 0 min and 1 h the sample was analyzed for concentrations of inorganic monophosphates with the ATPase/GTPase Activity Kit (Sigma-Aldrich, Cat. no: MAK113-1KT) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... they were dehydrated in an ethanol series (25, 50, 70, 100, and 100 %) and embedded in Epoxy resin using Epoxy medium kit (Sigma-Aldrich, St-Louis, MO, USA). Then ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was extracted from pooled seedlings at the EBI laboratories using the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: RNA was prepared for Iso-Seq using the Sigma Spectrum Plant Total RNA Kit including On-Column DNAse I Digestion (both products Sigma-Aldrich, St. Louis, MO, USA). One Hen17-A07 plant was sectioned into three parts (roots ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA of the evolved mutants was isolated from LB overnight cultures using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich St. Louis, MO). The extracted DNA was evaluated by gel electrophoresis and quantified by a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was collected and the amount of glucose in the medium was determined using the glucose assay kit (Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative Real-time PCR reactions were performed on a Bio-Rad CFX96 Termo-cycler with Kapa Syber Fast qPCR master mix (2x) kit (Sigma-Aldrich, Saint Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The nuclear extract was prepared from larval tissue (~20 larvae) of Canton-S stock using the CelLytic™ NuCLEAR™ Extraction Kit (Sigma, Missouri, Unites States). The nuclear extract was taken in different amounts and incubated in the presence of 1X binding buffer (250mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: The PLA assay was performed to determine the in situ binding between LC3 and ATG9a or ATG9b by using the Duolink® In Situ Red Starter Kit purchased from Sigma Aldrich (catalog number DUO92101), following the protocol provided by the manufacturer.
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bioRxiv - Genomics 2020Quote: ... Supernatant was aspirated before the isolation of single nuclei was performed using the Nuclei PURE Prep nuclei isolation kit (Sigma-Aldrich, St. Louis, MO, USA) in conjunction with a modified protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: Total RNA of the collected/dissected petals (see supplementary fig. S2) was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). cDNA was synthesized following the methods described in Supporting Information Methods S4 in Lin and Rausher (2021) ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was collected avoiding to disturb the pellet and was mixed with 750 microliters of Binding buffer of Total Spectrum RNA kit (Sigma–Aldrich, Saint Louis, MO, USA). Subsequently the extraction proceeded following manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: RNA extract from a single female adult tick was converted to cDNA using the Complete Whole Transcriptome Amplification Kit (WTA2, Sigma-Aldrich, Saint Louis, MO, USA). Purification of cDNA was done using the MSB Spin PCRapace kit (Stratec Molecular ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of WT BBS and BBS Mut MCF7 and HCC1806 cells were lysed using ice-cold lactate assay buffer provided in the Lactate assay kit (Sigma, MAK064-1, lot no. 3F09K06270). The lactate quantification was performed using the deproteinized lysates by following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 21 days-after-flowering (DAF) sunflower seeds using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Tissues were ground into a fine powder in liquid nitrogen and total RNA was extracted using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO). Sequencing libraries were produced using the TruSeq RNA Sample Preparation kit v2 (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of leaf powder was used for RNA extraction following protocol A of the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO). RNA was eluted in 50 μL elution buffer ...