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Citations for Millipore Sigma :
7401 - 7450 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: DNA extraction was performed for each of the above isolated samples by following manufacturer’s instructions using the GenElute™ Bacterial Genomic DNA kit (Sigma-Aldrich). After gDNA extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma, #12039672910). 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 x 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... which was then washed with up to 2.5 ml of digestion buffer (Tumour dissociation kit, Miltenyi, 130-096-730) plus Deoxyribonuclease I (300 μg/ml, Sigma, DN25-1G). Dissociation was performed using the protocol suggested by Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... total genomic DNA from each clone was extracted from 2mL of an overnight culture using the GenElute Bacterial Genomic DNA kit (Sigma-Aldrich) and the quality and integrity of the DNA was assessed on a 1% agarose gel and the concentrations were determined fluorometrically using Quant-iT™ PicoGreen® dsDNA Reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... mLGR5-eGFP or cLGR5-eGFP were used as target cells in the killing assay and were pre-loaded with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 2.5 × 106 or 6.25 × 105 NK92 cells or CAR expressing NK92 cells were used at indicated effector to target ratios ...
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bioRxiv - Cell Biology 2022Quote: ... equal number of DB-VOs and control ND-VOs were labelled by LuminiCell Tracker 670-Cell Labeling Kit (SCT011, Sigma-Aldrich), 0.8nm for 12 hours in Stempro34 complete medium ...
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bioRxiv - Microbiology 2024Quote: Thiol concentration of culture supernatants was measured using a fluorometric thiol assay kit according to the manufacturer’s instructions (Sigma-Aldrich, MAK151). Fluorescence was measured with a BioTek Synergy H1 microplate reader (Agilent ...
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bioRxiv - Bioengineering 2024Quote: ... very low- and low-density lipoprotein [(V)LDL] were separated from the plasma above using a HDL and LDL/VLDL Quantitation Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Cell Biology 2024Quote: ... live tachyzoites prepared as mentioned above were incubated with the lipid dye PKH26 to label the plasma membrane using the Cell Linker kit (041M0852, Sigma Aldrich) in 0.5 mL final volume following the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between CAMKV and CREB was assessed in SK-N-AS cells using an in situ PLA assay (Duolink in situ red starter kit, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... in FibroGRO™ Complete Media Kit supplemented with bFGF (75 ng/mL) and 2% of foetal bovine serum (FBS, Sigma-Aldrich). Only iPSC-cFbs with a passage number ≤ 10 (P10 ...
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bioRxiv - Molecular Biology 2024Quote: The enzymatic activity of rFhENO fractions purified by affinity chromatography and separated by SEC was assessed by a fluorogenic assay using the commercial Enolase Activity Assay Kit (MAK178; Sigma-Aldrich). The molar concentration of protein in each SEC fraction was determined using the Beer Lambert Law (A = ɛcl ...
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bioRxiv - Plant Biology 2024Quote: RNA of aerial and subterranean fruit valves (stage 16-17a and 17ab) was extracted from three biological replicates using SpectrumTM Plant Total RNA kit (Sigma-Aldrich). For long read sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA extraction with an on-column DNAse treatment was performed with the GenElute Mammalian Total RNA Miniprep Kit (Sigma Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Debris was removed with density gradient centrifugation using Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer from the Nuclei PURE prep isolation kit (no. NUC201-1KT, Sigma Aldrich). Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer were first mixed in a 9:1 ratio ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was assayed between 20-24 hours post-treatment for lactate dehydrogenase (LDH) activity as a marker of cell death using a commercially available kit (Tox7, Sigma-Aldrich) as previously described (Aras et al. ...
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bioRxiv - Pathology 2024Quote: The content of human IgG was quantitated both in the sera and aortic (tissue) homogenates from rats treated with chP3R99 or hR3 mAbs by a commercial ELISA kit (Sigma-Aldrich). Similarly ...
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bioRxiv - Biochemistry 2023Quote: Free and encapsulated DyP peroxidase (KpDyP and KpDyP_Enc) were purified and diluted to equimolar heme concentrations as determined via heme assay kit (Sigma-Aldrich, USA) per manufacturer protocol to analyze enzyme activity via ABTS assays ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Genetics 2023Quote: ... Duolink probes (mouse plus and rabbit minus) were prepared and applied as described by manufacturer’s instructions for the Duolink® PLA kit Cy5 (Millipore-Sigma DUO92013).
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bioRxiv - Biochemistry 2024Quote: Genomic DNA (gDNA) was extracted from cell pellets using the Sigma GenElute Mammalian gDNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO). DNA amplicons for Illumina sequencing were produced from the gDNA using a previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from ~30 mg of abaxial epidermis or the remaining leaf tissue using the Spectrum Plant Total RNA kit (Sigma-Aldrich) including on-column DNase I digestion (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were then washed 2x in PBS before performing proximity ligation reaction using the Duolink In Situ Red Starter kit (Sigma Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Serum iron and TIBC were determined using the Total Iron-Binding Capacity and Serum Iron Assay Kit (MAK394, Sigma Aldrich, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the mouse anti-DCP1A antibody was covalently conjugated to a 488 fluorophore using the Mix-n-Stain CF488A Antibody labeling kit (Sigma, MX488AS100) and used together with the rabbit polyclonal anti-ORF1p antibody covalently conjugated to a 647 fluorophore with the Mix-n-Stain CF647 Antibody labeling kit ...
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bioRxiv - Cell Biology 2023Quote: G6P and NADP+/NAPDH were measured from total worm extracts or cell extracts using the Glucose-6-Phosphate Assay Kit (Sigma MAK014) and the NADP/NADPH-Glo Assay (Promega G9081) ...
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bioRxiv - Cancer Biology 2023Quote: To assess HOXB13-SMARCD2 interaction in prostate cancer cells PLA was carried out using Duolink® kit (Cat # DUO92101, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... we prepared sucrose gradients with Nuclei PURE Sucrose Buffer and Nuclei PURE 2M Sucrose Cushion Solution from the Nuclei PURE Nuclei Isolation Kit (NUC-201, Sigma-Aldrich), and samples were carefully overlaid and centrifuged at 13,000 g for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... To evaluate SDH activity cells were stimulated as described above and SDH activity was analyzed with Succinate dehydrogenase Activity Colorimetric Assay Kit (Sigma-Aldrich) according to the manufacturers′ instructions.
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bioRxiv - Immunology 2023Quote: Five million cells were marked using the PKH-26 Red Fluorescent Cell Linker Kits (Sigma-Aldrich, catalog PKH26GL St. Louis, MO). Following the manufacture instructions ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human CD56 Positive Selection Kit II) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... The osteoclasts were fixed and stained with tartrate-resistant acid phosphatase (TRAP)-positive cells based on a leukocyte acid phosphatase kit (cat no 387A; Sigma; USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The interaction between AID and DIS3 was detected using Duolink In Situ PLA Probe Anti-Goat MINUS kit (Sigma-Aldrich, DUO92006) and Duolink In Situ PLA Probe Anti-Rabbit PLUS kit (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with PBS twice and the in-situ PLA was performed with the Duolink In Situ PLA kits (Sigma-Aldrich) according to manufacturer instructions with the exception that the reactions and washings were carried out in 1,5 ml microcentrifuge tubes ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA (gDNA) was extracted from overnight LB liquid cultures using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Plant Biology 2023Quote: ... ∼50 mg of tissue was ground under liquid nitrogen and the total RNA was ex-tracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) following the manufac-turer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: The cell toxicity of the drugs was determined using Cell Counting Kit-8 (CCK-8) (Catalog No.: 96992-100TESTS-F, Sigma-Aldrich) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal deletion mutants were verified by amplification of the genomic region from isolated genomic DNA (GeneElute Bacterial Genomic DNA Kit, Sigma Aldrich) and by whole genome sequencing (MicrobesNG/Plasmidsauraus) ...
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Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging responsebioRxiv - Plant Biology 2023Quote: ... The tissue was ground in liquid nitrogen and total RNA was extracted using Spectrum™ Plant Total RNA kit (Sigma-Aldrich). For each condition (waterlogged or untreated) ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA from mouse liver or striatum (see below) was extracted using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma #G1N350).
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bioRxiv - Physiology 2023Quote: ... Peroxidase-Glucose oxidase (PGO) enzyme plus color reagent was added to each well (Glucose Assay Kit: Sigma GAG020, PGO Sigma P7119), the plate was incubated at 37°C and the optic density was read at 450 nm using a Tecan Spark Microplate Reader ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Alkaline-phosphatase (Alk-Phos) positive cells were assessed on day 18 in culture using Leukocyte Alkaline Phosphatase Kit (Sigma; cat#86R) following manufacturer instructions.
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bioRxiv - Cell Biology 2023Quote: ... RPE cells were harvested from WT and Akt2 KI mice and ChIP was performed using a commercially available kit (17-295, EMD Millipore). The following primers for the Atg9b promoter and negative control were used Atg9B_CHIP_F1 ...