Labshake search
Citations for Millipore Sigma :
7651 - 7700 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... SAF-A:RNA complexes were detected in BrU-labeled cell populations using the rabbit α-SAF-A and mouse α-BrU antibodies in conjunction with the Duolink Proximity Ligation Assay kit (Sigma, DUO92102).
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bioRxiv - Cell Biology 2020Quote: ... PLA probes were applied in 1:10 dilution using the diluent buffer provided in the Duolink In Situ Red kit (Sigma Aldrich, cat# DUO92101). Incubations and subsequent ligation and amplification steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... in the case of young oothecae (from NFE to 4-day-old) and GenElute Mammalian Total RNA Miniprep kit (Sigma-Aldrich, Madrid, Spain) in the case of older oothecae (from 6- to 16-day-old) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 15 mg of the tissue from each mouse liver was used for genomic DNA extraction using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma Cat. #: G1N350).
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bioRxiv - Cell Biology 2022Quote: ... were grown in ATCC’s vascular cell basal medium supplemented with microvascular endothelial cell growth kit-VEGF and 12.5 μg/mL blasticidine (Sigma-Aldrich, St Louis, MO) for 48 h at 37°C + 5% CO2 until reaching 80-90% confluence ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then probed for phosphorylated STATs using the Milliplex map STAT cell signaling magnetic bead 5-Plex kit (Millipore Sigma, 48-610MAG) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... 5-10 μg protein lysates were cultured and proteasome activity measured using the 20S Proteasome Activity Assay kit (APT280, Millipore, Billerica, MA, USA) per manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from a 3 ml culture of the representative strain using the GenElute ™ Bacterial Genomic DNA Kit (Sigma Aldrich, Sweden) [7].
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bioRxiv - Physiology 2019Quote: ... leptin and insulin were analyzed by commercial ELISA’s according to manufacturer’s instructions (EIA CORT kit, Arbor Assays, Michigan, USA; adiponectin, insulin and leptin, Milliplex Mouse Adipokine Multiplex, Millipore, Amsterdam, The Netherlands).
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bioRxiv - Cell Biology 2019Quote: ... PLA probes were applied in 1:10 dilution using the diluent buffer provided in the Duolink In Situ Red kit (Sigma Aldrich, cat# DUO92101). Incubations and subsequent ligation and amplification steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... For ChIP-qPCR 10 µl of IP samples were amplified using the Genomeplex Single Cell Whole Genome Amplification Kit (WGA4) (Sigma Aldrich, Taufkirchen, Germany) and subjected to qPCR in a 1:40 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... and visualized by exposing the membrane to a charge-coupled device camera (LAS1000, Fujifilm, Tokyo, Japan) using a chemiluminescence kit (Merck Millipore, Billerica, MA, USA). Membranes were stripped and reprobed for β-actin (diluted 1:50 000 ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from ~100 mg of powdered tissue that was kept at −80 °C was extracted using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse I (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: In situ Proximity Ligation Assay (PLA) was performed with Duolink in situ Red Starter kit Mouse/Rabbit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore, 17-10086). Chromatin DNAs quality was assessed by 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Serial sections for trabecular bone were obtained from the distal femur at a thickness of 5 µm followed by tartrate-resistant acid phosphatase (TRAP) staining or alkaline phosphatase (ALP) staining using TRAP kits (Fast Red TR/Naphthol AS-MX, Sigma, St. Louis, MO) and NBT/BCIP (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... donor cells were labelled with CellVue Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labelling (Sigma Aldrich, Cat#MINCLARET-1KT) following manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Both cell lines were maintained in a 5% CO2 humidified incubator at 37°C and monitored periodically for mycoplasma contamination (Sigma Venor GeM Kit). Unless otherwise indicated ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was isolated using the Gene Elute Mammalian Total RNA Miniprep Kit according to the manufacturer’s protocol (Sigma–Aldrich, St. Louis, MO, USA). Concentration of the isolated RNA was determined photometrically with a BioSpectrometer® (Eppendorf ...
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bioRxiv - Immunology 2020Quote: ... GM-CSF and TNF-α using the Luminex kit according to the manufacturer’s instructions (Milliplex Multiplex Assays with Luminex, Millipore Sigma, Danvers, MA). Samples were assayed using the Luminex assay system (Magpix).
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bioRxiv - Molecular Biology 2020Quote: ... Densitometry was performed as previous described.19 Proximity ligations between ephrinb2/EphB4 in flow-aligned cells were conducted by using Duolink® In situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, MO). Numbers of individual ligations in raw and post-processed images were compared to test the validity of the quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Molecular Biology 2022Quote: Cell were fixed/permeabilized with BD Cytofix/Cytoperm™ Kit (Cat 554714) and stained with DAPI (4,6, diamidino-2-phenylindole, Sigma-Aldrich, Cat D9542). DAPI (1 in 100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the entire surface of the agar plate was scraped using a loop and deposited into 180 μl of lysis solution provided by the GenElute Bacterial Genomic DNA extraction kit (Sigma-Aldrich, Darmstadt, Germany). For approach 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These products were purified by running on a 1% agarose gel and gel extracted using the GenElute™Gel Extraction kit (Sigma-Aldrich, USA). The extracted bands were Sanger sequenced by Eurofins (USA ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2019Quote: ... Each viral DNA sample was then amplified using GenomePlex® Complete Whole Genome Amplification (WGA2) Kit from Sigma-Aldrich (Gudenkauf and Hewson, 2016). Two blank controls were included in this step ...
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bioRxiv - Immunology 2019Quote: Chitinase activity was analyzed by using the substrate and standard solutions of the fluorimetric chitinase assay kit (Sigma-Aldrich Chemie GmbH, Munich, Germany). Substrates 4-MU-(GlcNAc)2 (Exo-Chitinase activity ...
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bioRxiv - Systems Biology 2019Quote: ... The assay named ‘Other Luminex’ was performed only for study SLVP015 in 2007 using the Human 42-Plex Polystyrene Kit (EMD Millipore, H42; MPXHCYTO060KPMX42) and data was processed in the same way as for the Luminex assays described above (measurement units reported were Zlog2)28.
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from four leaf discs per replicate (three replicates, each sampled from two independent flood-inoculated ‘Hort16A’ plantlets) via a Spectrum™ Plant Total RNA kit (Sigma Aldrich, NZ). RNA was DNase I (Sigma Aldrich ...
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bioRxiv - Physiology 2020Quote: ... was assessed in serum/plasma collected from lean and obese ZSF1 rats at the age of 20 weeks by rat-specific MILLIplex kit (Millipore, Billerica, MA, USA). Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: Larvae were processed as described (121) using the DuoLink Mouse Rabbit in situ PLA Kit (Sigma-Aldrich, cat. no. DUO92101, St. Louis, MO). Controls were performed with one or the other epitope-tagged transgene absent or with the probes replaced by water during the first PLA step to ensure that signal observed as not background or bleed-through of channels ...
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bioRxiv - Microbiology 2020Quote: ... 200ml of culture grown to stationary phase was concentrated using centrifugation (27,000 × g for 15 minutes) and extracted with the GenElute Bacterial Genomic DNA Kit (Millipore Sigma, Burlington, MA, USA). Basecalling was done using Guppy version 2.2.3 and long reads were filtered using filtLong version 0.2.0 (https://github.com/rrwick/Filtlong) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA for both root and shoot tissues was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Alridch, Carlsbad, California, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was discarded and supernatant was used to analyze the GST activity (Thounaojam et al. 2012). GST activity was performed according to method (Habig et al. 1974) by utilizing GST Assay kit (Sigma, USA, CS0410-1KT)
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bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the B protocol of the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan, Italy https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/nxtractbul.pdf), in order to obtain native nuclear proteins ...
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bioRxiv - Biochemistry 2020Quote: ... concentrations in the different culture media were determined by the Bio-Plex method using the mouse adipocyte magnetic bead panel kit (Millipore, MADCYMAG-72K-07), according to manufacturer’s instructions (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of wild-type LonP1 and its mutants was measured with ATPase/GTPase activity assay kit (Sigma-Aldrich, USA, product: MAK113) as per the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Sample preparation - In-gel digests were performed for each excised sample using the ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212; Calbiochem, EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Masson’s trichrome Staining was applied according to the manufacturer’s instructions of the Trichrome Stain (Masson) Kit (HT15-1KT, Sigma-Aldrich, Heatherhouse, United Kingdom). For Safranin O/Fast green staining ...
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bioRxiv - Immunology 2021Quote: ... The pyrosequencing assay was validated using SssI-treated human genomic DNA as a 100% methylation control and human genomic DNA amplified by GenomePlex Complete Whole Genome Amplification kit (Sigma-Aldrich, WGA2-50RXN) as 0% methylation control ...
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bioRxiv - Microbiology 2022Quote: ... The iron content of pre and post Chelex® 100 treatment was determined using the Iron Assay Kit (Sigma-Aldrich, MA, United States) following the manufacturer’s recommendations (Table S5).
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bioRxiv - Cell Biology 2022Quote: ... Conditioned media was run on the MilliPLEX Human High Sensitivity T Cell Magnetic Bead Panel Luminex kit for measurement of 21 unique cytokines per plex according to manufacturer’s instructions (EMD Millipore, St. Louis, MO).
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bioRxiv - Microbiology 2022Quote: ... Serum cytokine levels were measured by the Indiana University Multiplex Analysis Core using a Millipore Milliplex MAP Mouse Cytokine/Chemokine Magnetic Kit (Millipore Sigma, Burlington, MA). Undiluted serum was provided to the Indiana University Multiplex Analysis Core and ran according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Gram staining of the LT0009 isolate was performed using a Gram-staining kit according to the manufacturer’s instruction (Sigma Aldrich, 77730-1KT-F) and its cellular morphology was imaged with a scanning electron microscope (JSM-IT300 ...