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Citations for Millipore Sigma :
7701 - 7750 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The senescence condition in the two cellular models was assessed by monitoring the morphological changes and by β-galactosidase (β-gal) staining with the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich). After reaching the preferred condition ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in biotinylated anti-rabbit secondary antibody 1:500 (Vectors Laboratories, #BA-1000) followed by Avidin/Biotin complex amplification (Vectastain Elite ABC kit; Vectors Laboratories, #PK-6100) and DAB (Sigma Life Science, #5905) mixed with 3% hydrogen peroxide for signal amplification ...
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bioRxiv - Microbiology 2024Quote: To levels of cytokines in the sera of infected animals were obtained using the MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay Kit (Merck-Millipore, BCYT1-33K) and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... Medium containing supplements were re-fed every 3-4 days for a total of 12 days after which the cells were evaluated for morphological changes and stained for tartrate-resistant acid phosphatase (TRAP) with a commercially available leukocyte acid phosphatase kit (SIGMA, Cat. 387-A). Three replicates of differentiated cells were processed to prepare samples for ATAC-seq at 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
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bioRxiv - Bioengineering 2024Quote: ... Imaging signal was visualized using imaging kits (SuperSignal™ West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate, Sigma-Aldrich) via an Image Quant LAS 4010 chemiluminescence imager (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Immunology 2023Quote: Cells from day 5 bone marrow cultures from tet-LIN28B or littermate control mice were used for lysis and RIP using Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, cat#17-700) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... leptin and insulin levels were measured in 12 h-fasted mice using a Mouse Metabolic Hormone Magnetic Bead-based immunoassay kits (Millipore Corp, Billerica, MA) according to the manufacturer instructions on samples obtained at the sacrifice.
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bioRxiv - Immunology 2023Quote: Chitinase activity in the culture supernatant of CHIT1 plasmid-transfected HEK293T cells was measured by using a fluorescence assay kit (Sigma-Aldrich Cat. # CS1030). A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 30–100 mg of ground (with liquid nitrogen) petal tissues using the Tri-Reagent Kit (Sigma-Aldrich, https://www.sigmaaldrich.com) and treated with RNase-free DNase I (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: Nuclei were extracted from flash-frozen leaf tissue using a Sigma CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma-Aldrich, Burlington, Massachusetts). Crosslinking was performed following low-input protocols from Arima Genomics (Arima Genomics ...
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bioRxiv - Biochemistry 2023Quote: ... Cell toxicity was measured in 96-well plates (∼5×104 cells/well) using a lactate dehydrogenase (LDH) cytotoxicity kit (Sigma-Aldrich, Cat # 11644793001). Absorbance was measured at λ=490 nm ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: Cytokine concentration levels of 5xFAD hippocampal homogenate samples were quantified using the Milliplex Map Mouse Cytokine/ Chemokine Magnetic Kit (Millipore MCYTMAG-70K-PX32), measuring a broad panel of secreted cytokines ...
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bioRxiv - Genomics 2023Quote: ... The culture medium was then aspirated and cells were lysed by adding 600ul buffer RLT from RNeasy Mini kit with 1:100 V/V β-mercaptoethanol (Sigma M3148-250ML) to the dish ...
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bioRxiv - Plant Biology 2023Quote: Total RNA from different tissues was extracted from 100 mg tissue using the TRIzol reagent or SpectrumTM Plant Total RNA kit (Sigma-Aldrich, United States) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Measurements of phosphate concentration in the growth medium supernatant were done using a Malachite green phosphate assay kit (Sigma-Aldrich, Cat. No. MAK307). After collecting the supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: Whole-thorax samples were grounded into a fine powder using a TissueLyser and used as input for the SpectrumTM Plant Total RNA Kit (Cat. No. STRN50, Sigma Aldrich, MO, USA), including DNase I treatment (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second parotid salivary gland was analyzed using the pyruvate kinase activity assay kit (cat. No. MAK072, Sigma-Aldrich, St. Louis, MO). For both activity assays ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with siRNAs/plasmid as described earlier and RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma Aldrich, # RTN70). RNA was reverse transcribe using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814) ...
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bioRxiv - Developmental Biology 2024Quote: ... the cultures were washed 3 times in PBS for 5 minutes and incubated with Duolink Plus and Minus PLA probes (diluted 1:5) followed by Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, cat. no. DUO92102), according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... lysates were subjected to a Millipore Luminex based Multiplex assay to measure signaling targets using the Milliplex multiple pathway cell signaling magnetic bead 9-Plex kit (Millipore Sigma, 48-681MAG) according to manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: The total RNA from separate achene and receptacle at six development stage with three replications per sample were extracted by the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, MO, USA) as fallowing the manufacturer protocol ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA was isolated from the base of spruce hypocotyls (5 mm long from 36 to 38 hypocotyl cuttings) using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich; https://www.sigmaaldrich.com/). cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Bio-Rad ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Molecular Biology 2019Quote: Protein from whole-tissue sample lysates (40 μg) were batch processed for 20S proteasome activity using commercially available fluorometric kits (catalog #: APT280; Millipore Sigma; Burlington, MA, USA) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: The emtA gene of strain DHT3 was disrupted by an intragenic insertion of a group II intron into this gene using the TargeTron Gene Knockout System Kit (Sigma-Aldrich, St. Louis, MO). The intron insertion sites for emtA were predicted using online intron design software (TargeTron gene knockout system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we also determined whole-body triacylglyceride (TAG) content (in µg per fly) using a serum triglyceride determination kit (Sigma Aldrich; Tennessen et al. 2014). For each cage and treatment ...
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bioRxiv - Immunology 2019Quote: ... PCs and B cells were detected by flow cytometry by combining surface staining with intracellular staining using the Edu-labelling kit following the manufacturer’s instructions (Sigma, EdU-Click 488/Base click).
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bioRxiv - Bioengineering 2019Quote: ... and TIRAP (Novus NB300-990, 1:100 dilution) and analyzed via flow cytometry using a DuoLink PLA flow cytometry kit (Sigma Aldrich, St. Louis, MO). Samples were analyzed using a BD Accuri C6 Benchtop Cytometer and median fluorescence intensity was reported.
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from up to 100 mg of liquid nitrogen ground tissue using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions for protocol B ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no. HCVD3-67CKHCYTOMAG-60K, Millipore Corp, St. Charles, MO). According to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Tissue samples were frozen in liquid nitrogen and total RNA was extracted with the Spectrum Plant Total RNA Kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA isolation of the retina was carried out according to the manufacturer’s introduction using the Gene Elute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA). For total RNA isolation of optic nerve tissue ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Cell Biology 2021Quote: ... the hiPSC-CMs were fixed and permeabilised with the FIX and PERM™ Cell Permeabilization kit and labelled with an antibody specific for α-actinin (1:250, Sigma-Aldrich #A7811), followed by an Alexa Fluor 350-conjugated secondary antibody (1:500 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from callus fragments grown on selective medium for eight weeks using a commercial kit (Sigma, Spectrum™ Plant Total RNA). First strand cDNA was synthesised using polyA primers and RevertAid First Strand cDNA synthesis kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: The release of inorganic phosphate during 2C-mediated ATPase hydrolysis was measured using the Malachite Green Phosphate Assay Kit (Sigma Aldrich, Zwijndrecht, The Netherlands). Each 50 µL reaction comprised 500 nM recombinant 2C protein and 1 mM ATP in reaction buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... and ground to a fine powder (100-200 mg) prior to RNA isolation using the Spectrum™ Total Plant RNA Kit with DNase I digestion (Sigma, St. Louis, MO). The only modification to manufacturer’s ‘Protocol B’ was that prior to the tissue lysis step ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the genomic DNA (gDNA) from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Physiology 2022Quote: The mNGF level in tears upon a AAV2/9 CAG-mNGF injection in the LG was dosed using the mNGF ELISA kit (Sigma-Aldrich, reference RAB1119, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... total cellular triglycerides were extracted from cells using the Folch extraction method and quantified by using a Serum Triglyceride Determination Kit (Sigma, St. Louis, MO, USA). The absorbance was read at 540nm using an Eppendorf Biospectrometer and 2.5 mg/mL glycerol was used as the standard (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration of the supernatant was determined by comparison to a standard curve of bovine serum albumin (BSA) solutions using the bicinchoninic acid (BCA) assay kit (Catalog No. BCA-1, Sigma, St. Louis, MO, USA) and absorbance was read at 562 nm using a BioTek Synergy HT plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was collected and prepared for glycogen measurement alongside a 2 mg/mL glycogen standard using the Sigma glycogen assay kit (Catalog No. MAK016, Sigma, St. Louis, MO, USA) and read using a BioTek Synergy HT plate reader at 570 nm.
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bioRxiv - Biochemistry 2021Quote: ... Each cellular fraction was then expanded and harvested prior to the extraction of genomic DNA using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A semi-nested PCR approach was then used to selectively amplify the mutagenized region of the polyprotein reporter from the genomic integration site in a similar manner as was previously described (Matreyek et al. ...