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Citations for Millipore Sigma :
7451 - 7500 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: PLA: PLA was carried out using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, Cat #DUO92101-1KT). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: The effects of MALAT1 siRNA on hMSCs proliferation were characterized with a cell proliferation assay (Cell Counting Kit-8, CCK-8 assay, Sigma Aldrich). hMSCs were seeded in a flat-bottom 96-well tissue culture plate at a concentration of 2000 cells per well with a volume of 100 µL ...
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bioRxiv - Physiology 2023Quote: ... the supernatant and the fasting and 15 min GTT serum was performed using Rat/Mouse Insulin ELISA kit (EZRMI-13K, Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Total genomic DNA from cecal digesta was extracted using PowerSoil® DNA Isolation Kit (Sigma-Aldrich, Carlsbad, United States of America). DNA concentration and purity were assessed using a Synergy HTX (Gene Company Limited ...
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bioRxiv - Immunology 2023Quote: Inflammation-associated with neutrophils and monocytes tissue infiltration was indirectly determined by measuring the MPO activity in 2 cm length fragments of distal colon using an MPO colorimetric activity kit assay (Sigma-Aldrich), following the manufactures instructions.
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Cell Biology 2023Quote: ... For signal amplification the ABC Kit (Vector, Burlingame, CA, USA) was used before applying 3,30-diaminobenzamidine (Sigma-Aldrich, St Louis, USA) as a chromogen ...
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bioRxiv - Biochemistry 2023Quote: Duolink proximity ligation assays were performed using a Duolink™ In Situ PLA Kit Goat/Rabbit (Olink Biosciences) purchased from Sigma. Briefly ...
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bioRxiv - Microbiology 2023Quote: ... the resulting powders were employed for total RNA extraction with “Spectrum Plant Total RNA” kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s protocol (20).
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bioRxiv - Microbiology 2023Quote: ... the reactions were heated to 90 °C for 10 minutes and then allowed to gradually cool to room temperature and RNA was purified using the GenElute Mammalian Total RNA kit (Sigma RTN350) according to the manufacturer’s protocol ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... 4-octanol used in the GC analysis and amylase activity assay kit were purchased from Sigma-Aldrich (Castle Hill NSW, Australia). Micro bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... samples were aspirated from each well and kept on ice for analysis with a colorimetric glutamate assay kit (Sigma-Aldrich; MAK004), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: In situ proximity ligation assays (PLAs) were performed using Duolink in situ red starter kit according to the manufacturer’s instructions (Millipore Sigma, DUO92101). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from plant tissue samples using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St Louis, MO, USA) followed by DNase digestion with RNase free DNase Set (Qiagen ...
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bioRxiv - Biophysics 2023Quote: The ATP hydrolysis activity of ChlI was assessed by quantification of free orthophosphate using the malachite green phosphate assay kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Molecular Biology 2023Quote: ... Migrated cells on the bottom side of the membrane were either stained with crystal violet or were collected by trypsination and counted using a cell titer blue staining kit (CTB; Sigma-Aldrich). For measuring invasion ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the PEG/Lithium acetate method (Yeast Transformation Kit, MilliporeSigma)(Gietz and Woods 2002) and plated on non-inducing selective synthetic media (Sigma-Aldrich® Yeast Synthetic Drop-out Media Supplements ...
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bioRxiv - Genetics 2024Quote: ... digestion at 37 ᵒC for 20 minutes and used directly for bisulfite treatment using the Imprint DNA Modification Kit (Sigma MOD50) according to the manufacturer’s 2-step modification procedure ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET ...
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bioRxiv - Cell Biology 2024Quote: ... The Acetyl CoA content was then determined using the Acetyl coenzyme A assay kit as per the instructions of the manufacturer (Sigma-Aldrich). An Acetyl-CoA standard curve was plotted in the range of 0–100 pmol ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Cancer Biology 2023Quote: ... the lyophilized powder was reconstituted with deionized (DI) water and processed using the Sigma Glutamate Assay Kit (catalog no: MAK330, Sigma Aldrich) while the protein content was measured using the Pierce™ BCA Protein Assay Kit (catalog no ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue triglyceride assays were performed as previously described using a Triglyceride Quantification Colorimetric/Fluorimetric Kit (Cat. no. MAK266 Sigma-Aldrich, Inc.) (23).
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bioRxiv - Cell Biology 2023Quote: ... A fraction of the cell lysates was used to estimate NAD(H) levels using the NAD/NADH quantification kit (Sigma MAK037) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... The p-nitrocathecol standard was prepared according to the manufacturer’s protocol for the sulfatase activity assay kit (Sigma, Cat# MAK276-1KT). Absorbance readings at 515 nm were obtained from a Bioteck Synergy HT plate reader ...
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bioRxiv - Synthetic Biology 2023Quote: The segregation analysis was performed on genomic DNA extracts of Synechocystis and Synechocystis mutants obtained using the Gen-Elute Bacterial Genomic DNA Kit (Sigma-Aldrich) by effectuating a screen for GGR and ShMKS incorporation into slr0168 using PCR ...
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bioRxiv - Neuroscience 2023Quote: Nuclei were extracted from mouse frontal cortical tissue using the Nuclei PURE Prep kit (NUC201-1KT, Millipore Sigma, Burlington MA, USA) with volumes reduced to a quarter of the recommended amount ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Microbiology 2023Quote: A fresh culture of ULC029 in BG11 was used to extract genomic DNA using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) following the recommendations of the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Bioengineering 2023Quote: ... This cell inoculated hydrogel precursor was injected into central tissue lane of MFOC devices and incubated for 30 minutes prior to filling the side channels and media reservoirs with endothelial cell growth medium (VEGF kit) containing 25 µg/mL aprotinin (Sigma-Aldrich) to inhibit fibrin degradation.
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from different vegetative and reproductive developmental stages using SpectrumTM Plant Total RNA isolation kit according to manufacturer’s instruction (Sigma-Aldrich, USA). The quality of isolated total RNA was determined in a Nanodrop (ND-1000 ...
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bioRxiv - Plant Biology 2024Quote: ... To quantify the concentration of purified protein in the samples a Bicinchoninic acid (BCA) assay was conducted using the BCA kit following the manufacturer recommendations (Sigma Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... was performed to detect the interactions between ITGβ1 and CD44 using Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Developmental Biology 2024Quote: ... immune cell samples were first heat inactivated at 70°C for 10 mins and then subjected to metabolite analysis using GOD-POD kit (Sigma#GAGO20) and Triglyceride assay kit (Sigma#T2449 ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Unbound dye was removed using dialysis against a 1000-fold excess of PBS or Broccoli aptamer buffer at 4 °C overnight with the Pur-A-LyzerTM Mini 6000 Dialysis Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Recovered adherent cells were resuspended in MSC medium after centrifugation and counted using the GuavaEasycyte 8HT apparatus in Muse Count & viability kit (MCH600103, Cytek Biosciences) or with trypan blue (T8154, Sigma-Aldrich). Part of cells were frozen at 1×106 cells/vial in FBS containing 10% DMSO ...
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bioRxiv - Genetics 2024Quote: ... The ADP/ATP ratio was quantitatively measured from lysis supernatant using the Sigma ADP/ATP Assay Kit (Sigma-Aldrich, MAK135-1KT), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the supernatant was diluted and subjected to H3K9me3 ChIP using the ChIPAb+ Trimethyl-Histone H3 (Lys9) kit (Millipore, cat# 17-10242). Bound material was recovered after incubation with 15 μL of protein G beads (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed in tNCC or SHEPMYCN cells after 24 h Dox induced overexpression of MYCN using a Duolink PLA kit (Sigma, DUO92014) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... digoxygenin (DIG)-labeled antisense probes were prepared using the Roche DIG labeling kit (Sigma Cat # 11277073910 and T7 (Sigma Cat # 10881767001) or T3 (Sigma Cat # 11031163001 ...
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bioRxiv - Neuroscience 2024Quote: ... “Indirect” PLAs for nitrated hASYN and nitrated NDUFB8 were performed using probes already conjugation to secondary antibodies and provided in a PLA kit (Duolink, Sigma-Aldrich). Primary antibodies used for indirect PLA were ...