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Citations for Millipore Sigma :
7501 - 7550 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... the expression of early differentiation marker (alkaline phosphatase, ALP) and late-stage differentiation marker (calcium mineralization) were quantified using ALP staining kit (Sigma Aldrich) and Alizarin Red S (ARS ...
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bioRxiv - Cell Biology 2024Quote: ... β-galactosidase (X-gal) lung staining was performed on frozen lung sections using the Senescence Cells Histochemical Staining Kit (Sigma CS0030) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: The interaction between PRDX6 and SEPHS2 in SK-N-DZ WT and SK-N-DZ PRDX6 KO cells was observed using Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma- Aldrich) in the presence or absence of 600nm Selenocysteine ...
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bioRxiv - Developmental Biology 2024Quote: ... and pCMV-Sport6.ccdb-marcksl1.L were linearized with EcoRV followed by synthesis of anti-sense RNA probes using the digoxigenin RNA Labeling Kit (Sigma Aldrich) and SP6 polymerase ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 20 mL deionized water and dialyzed using the Pur-A-Lyzer Mega 3500 Dialysis Kit (Sigma-Aldrich) and lyophilized ...
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bioRxiv - Cell Biology 2020Quote: Chromatin from D5 control MEFs or D7 reprogrammed MEFs was isolated using the Magna ChIP A/G Chromatin Immunoprecipitation kit (Millipore 17-10085) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The proteins were resuspended and passed through EndoTrap® red endotoxin removal kit (LIONEX GmbH, Germany) and stored in endotoxin free water (Cat. No.#95289-1L, Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... Serum cytokine profiling targeting 22 cytokines/chemokines was performed by Public Health England using a 22 -plex customized Luminex kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from each three biological replicates of 100 mg pollen grains/pollen tubes by using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was used to assay for ANP levels using a competitive enzyme Immunoassay kit from Sigma-Aldrich (Catalog Number RAB0385). The levels of ANP were normalized for the amount of protein ...
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bioRxiv - Molecular Biology 2020Quote: The ATPase activity assay was performed in 96-well microtitre plates by using an ATPase/GTPase activity assay kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... Colonies were picked and grown in 5 ml liquid SD-L-W medium and the prey vectors were isolated using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, USA) after cell wall disruption ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Colorimetric assay to measure alanine aminotransferase activity in the cell lines was performed using the ALT activity assay kit from Sigma (MAK052-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Genomics 2020Quote: ... Chromatin was prepared using the MAGnify Low Cell ChIP-Seq Kit (Thermo Fischer) and ChIP was done for H3K4me3 (Millipore, # 07-473), H3K27ac (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Individual wells from cultured cells and tissue from mice was collected and then the homogenate was fractionated utilizing a NXTRACT CelLytic™ NuCLEAR™ Extraction Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Positive bacteria clones were inoculated into 50ml LB/carbnecillin (100 μg/ml) culture for amplification and midiprep plasmid purification using GenElute™ HP Plasmid Midiprep Kit (NA0200, Millipore Sigma). All plasmids except T3D-L1 were amplified at 37°C for 14-16hrs ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained for tartrate-resistant acid phosphatase (TRAP) to examine osteoclast activity and numbers (Sigma-Aldrich Kit, St. Louis, MO, USA), and Fast Green counterstaining and examined with a Nikon Eclipse 90i Advanced Research Scope ...
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bioRxiv - Genetics 2019Quote: Quantitative amplification was performed according to manufacturer’s specifications using KAPA SYBR® FAST qPCR Kit Master Mix (2X) Universal (Sigma, cat. #07959397001). 12 separate RNA isolations were analyzed ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... R&D Systems cytokine kits (Minneapolis, MN) or a high throughput multiplex cytokine assay system (Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, Millipore, MO, USA) was used for cytokine quantification ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated for 2 hours by the Autophagy Reagent A (rapamycin containing) from the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (Millipore, FCCH100171), which have been diluted at 1:50 as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Bioengineering 2020Quote: ... The alkaline phosphatase (AP) staining was performed 72 hours after seeding using the Leukocyte Alkaline Phosphatase kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... after 48 hours of incubation at 37°C were performed according to the manufacturer’s instruction (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Up to 100 mg of liquid nitrogen ground tissue was applied to the SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions for protocol B ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using a method combining CTAB lysis buffer and the Spectrum Total Plant RNA extraction kit (Sigma, Darmstadt, Germany) as described previously (83) ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50μg of lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from Legionella strains cultured for 18 h in AYE medium using the GenElute™ Bacterial Genomic DNA Kit (NA2100, Sigma-Aldrich) and used as template for PCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... Double-stranded cDNA was synthesized and amplified using a TransPlex whole-transcriptome amplification kit (WTA-2; Sigma-Aldrich, St. Louis, MO, USA) and antibody-inactivated hot-start Taq DNA polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Sulfate was determined via the barium precipitation method using the Sulfate Assay Kit following manufacturer’s instructions (Sigma Aldrich, Saint Louis, MI, USA). Sample measurements were carried out in technical triplicates.
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bioRxiv - Physiology 2021Quote: The NEFAS concentration was quantified using the Free Fatty Acid Quantitation Kit (MAK044) according to the technical bulletin (Sigma-Aldrich, United States). The results are expressed in ng/μl.
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bioRxiv - Cell Biology 2021Quote: Ect were labeled with PKH26 dye (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma Aldrich, cat. No. PKH26GL) as previously described [35] ...
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bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... organelle and nuclear fractions were harvested every 12 h for 2 days using the Nuclei EZ Prep nuclei isolation kit (Sigma NUC-101) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... for amplifying the whole genome of each lysed tissue sample by the GenomePlex Single Cell Whole Genome Amplification Kit (WGA4-50RXN, Sigma-Aldrich, USA). For each sample ...
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bioRxiv - Cell Biology 2021Quote: Golgi apparatus was isolated from mouse liver or cultured HCs by sequential sucrose gradients using a Golgi Isolation Kit (GL0010-1KT, Sigma-Aldrich, USA), based on the protocol we previously established (Tang and Wang ...
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bioRxiv - Microbiology 2021Quote: ... Following further dehydration in a graded acetone series the agarose blocs were embedded in Spurr’s resin (Spurr’s Low Viscosity embedding kit, Sigma Aldrich, St Louis, USA) using acetone as intermedium ...
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bioRxiv - Developmental Biology 2022Quote: ... In situ hybridization was completed according to a standard protocol (Cunningham and Monk, 2018) using DIG RNA labeling kit T7/SP6 (Millipore Sigma #11175025910). RNA probes were cleaned using SigmaSpin Sequencing Reaction Clean-UP (Millipore Sigma #S5059-70EA) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Perifusate fractions were collected at the flow rate of 3ml/min and insulin was measured using radioimmunoassay kit (Millipore, Cat #: RI-13K). Insulin secretion were normalized to IEQ.
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bioRxiv - Biochemistry 2022Quote: ... The Duolink procedure was conducted according to the manufacturer’s instructions with the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Duolink®, Sigma□Aldrich), with secondary antibodies contained in the kit ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The genomic DNA of kanamycin-sensitive and chloramphenicol-sensitive clones was isolated using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich), and analyzed by PCR to confirm the gene replacement using primers pairs TP_ctg3-43_f / TP_ctg3-43_r in reactions (20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...