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Citations for Millipore Sigma :
7351 - 7400 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... then stained with 1% Crystal Violet in 2% ethanol for 20 min for NIH3T3 cells and MCC cells were counted using a Cell Counting Kit-8 (CCK-8) (Sigma-Aldrich). The stained cells on the lower side were counted under a microscope from 5 different randomly selected views ...
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bioRxiv - Microbiology 2021Quote: In situ proximity ligation assay (PLA) was performed using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: Preparation of nuclear and cytoplasmic fractions was performed according to the user manual supplied with the CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma) with minor modifications ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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bioRxiv - Immunology 2020Quote: ... post-fixed with 1% osmium tetroxide and mordanted with 1% tannic acid followed by dehydration through an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (all from Sigma-Aldrich). Ultrathin sections cut on a Leica UC6 ultramicrotome were contrasted with uranyl acetate and lead nitrate ...
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bioRxiv - Microbiology 2019Quote: ... Glycerol content for all supernatant samples was measured simultaneously in a single 96-well plate using a Glycerol Assay Kit (Sigma-Aldrich) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... DNA was extracted from wild type Ps LMG5084 and its PL1-insensitive mutants using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). Libraries were prepared with the NEBNext Ultra II library kit according to the manufacturer’s instructions and sequenced on the Illumina HiSeq X platform to obtain 150 bp paired end reads with an average depth of 40-fold ...
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bioRxiv - Physiology 2021Quote: The BHB concentration was quantified using the Beta-Hydroxybutyrate Assay Kit (MAK041) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of BHB was expressed in ng/μg protein.
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: M6A enrichment followed by qRT-PCR was used to quantify the changes in m6A methylation of the target gene using the Magna MeRIP m6A Kit (Millipore, MA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Tissue powder was then resuspended in a lysis buffer from the Mammalian Cell Lysis kit containing a protease inhibitor cocktail (Sigma-Aldrich). Samples were incubated in lysis buffer at 4°C for 15 min rotating and then centrifuged for 20,000 x g for 10 min at 4°C to pellet cell debris ...
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bioRxiv - Physiology 2021Quote: ... extra blood was collected at each time-point and later plasma was separated by centrifugation (15min at 2,000 RPM) and stored at −80 °C for insulin measurement using a Rat/Mouse Insulin Elisa kit (EMD Millipore - EZRMI13K). Prior to sacrifice ...
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bioRxiv - Microbiology 2019Quote: ... 25 μl of the supernatant were hydrolyzed and processed as described in the MAK016 assay kit instructions for colorimetric assays (Sigma-Aldrich). For the replenishment experiment ...
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bioRxiv - Microbiology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink In Situ Red Started Kit Mouse/Rabbit (Millipore Sigma, Cat# DUO92101-1KT) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... Both the antibodies targeted against Imp-β1 and puromycin were conjugated directly to the PLA probes as PLAplus and PLAminus respectively using Duolink™ In Situ Probemaker PLUS and MINUS kits (Sigma). Neurons were then fixed with 3.2 % PFA before processing with immunocytochemistry.
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation).
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bioRxiv - Microbiology 2020Quote: Nuclear extracts (using NE-PER™ nuclear Extraction Kit (Thermo Fischer Scientific, USA) and whole-cell lysates (using RIPA buffer, Sigma) were prepared from MTB-infected macrophages (MOI 20:1) ...
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Microbiology 2020Quote: GlgE activity was monitored quantitatively in the elongation direction by the release of orthophosphate using the Malachite Green Assay Kit (Sigma-Aldrich) following the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: ... post-fixed with 1% osmium tetroxide and mordanted with 1% tannic acid followed by dehydration through an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (all from Sigma-Aldrich). Semi-thin 0.5 μm sections were cut and collected onto clean glass slides and dried at 60°C before staining with 1% Toluidine Blue and 1% Borax (all from Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and cloned into the mid-copy T7-FNS2 phage display vector as described previously,17 and packaged using the T7 Select Packaging Kit (EMD Millipore). Library quality assessment was performed as described in Results.
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bioRxiv - Microbiology 2020Quote: Phosphatase and kinase activities of SiaA and/or SiaB on SiaC and/or SiaCp were determined using the Malachite green phosphate assay kit (Sigma Aldrich) and ADP-Glo™ assay kit (Promega ...
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Treatment history shapes the evolution of complex carbapenem-resistant phenotypes in Klebsiella spp.bioRxiv - Microbiology 2022Quote: Genomic DNA of the Klebsiella isolates was prepared from solid media scrapings of pure culture using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) and the Gram-negative bacteria protocol ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were analyzed on a 1.2 % agarose-gel and extracted with Kit Montage Gel Extraction columns (Millipore, Bedford, MA). The purified amplicons were used in a BP-reaction for the creation of Gateway-Entry vectors using 1 μL purified amplicon ...
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: ... sections of the flap were frozen at - 80°C and processed [24] to measure the lactate content of the tissues using a Lactate assay kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Activity assays to see the inhibitory effect of Halo Library fragment binders on RT52A activity were performed using the Roche colorimetric assay kit purchased from Sigma Aldrich, using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Cell Biology 2022Quote: Fresh-frozen sections of young and aged bladders were tested for acid phosphatase activity using an acid phosphatase assay kit (CS0740-1KT, Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 or TFCP2L1 in the case of those imaged for confocal analysis ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (# C10428, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated by phenol chloroform extraction and alcohol precipitation followed by two consecutive rounds of poly-A selection using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm lengths of leaf tip) using the ‘Crude Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich). DNA extraction from crude nuclei was performed using two different methods ...
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Microbiology 2022Quote: ... while spotting them onto a polished stainless steel MALDI target in a 1:3 ratio (0.5 μL sample/1.5 μL matrix) with addition of 0.25 μL ProteoMass Normal Mass Calibration Mix (1:10 dilution, ProteoMass™ MALDI Calibration Kit, Sigma-Aldrich) for acquisition of high resolution spectra ...
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bioRxiv - Microbiology 2022Quote: The activity of the MtPAPP was determined by the production of orthophosphate which was quantified using the Malachite Green Phosphate Assay Kit (Sigma-Aldrich) by forming a green complex ...
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bioRxiv - Microbiology 2022Quote: ... 12 and 16 hours of bacterium growth for 24 hours at 37°C with shaking (180 rpm) using the Iron Assay Kit (Sigma-Aldrich). TECAN infinite M200PRO microplate reader was used for measuring Colorimetric readings.
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bioRxiv - Pathology 2022Quote: ... One ug total RNA was used for first strand cDNA synthesis of all samples Transcriptor High Fidelity cDNA kits (Sigma Aldrich) was used for first strand cDNA synthesis of all samples ...
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bioRxiv - Pathology 2022Quote: ... Leaf tissues were ground and homogenized in liquid nitrogen for subsequent total RNA isolation with the SpectrumTM Plant Total RNA Isolation Kit (Sigma, USA), followed by on-column DNase I digestion treatment (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ATP levels in media from hydroponic infection experiments of hydroponically-grown plants were analyzed using the ATP Colorimetric/Fluorometric Assay Kit (Sigma, USA) and an Infinite M1000 plate reader (Tecan ...
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bioRxiv - Plant Biology 2023Quote: ... The 100 frozen leaves for each of these sample and the positive sample were extracted in pools of 25 following the Spectrum plant total RNA kit (Sigma-Aldrich). RNA extracts were tested by RT-PCR using the Titan One Tube RT-PCR kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from the original 100 frozen leaves of the positive sample were re-extracted in pools of 25 with the Spectrum plant total RNA kit (Sigma-Aldrich) and tested by RT-PCR using the Titan One Tube RT-PCR kit ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...
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bioRxiv - Microbiology 2024Quote: ... Acetaldehyde content was further quantified using the supernatant of WT and ΔmftG grown in HdB-Tyl supplemented with 10 g L−1 ethanol alone and 10 g L−1 glucose combined with 10 g L−1 ethanol for 48 h as described in the acetaldehyde Assay Kit (Sigma-Aldrich) by the manufacturers.