Labshake search
Citations for Millipore Sigma :
7551 - 7600 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 200 mg of pollen tubes using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). RNA-seq libraries were performed using the non-stranded mRNA Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... DNase I treatment was given to avoid genomic DNA contamination followed by purification of total RNA using GenElute™ Total RNA Purification Kit (Sigma-Aldrich). The purified RNA was quantified using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Neuroscience 2020Quote: ... we spun down for 3 min at 13.000 rpm at 4° C and performed glucose assays on the supernatant using a Glucose (GO) assay kit from Sigma (GAGO-20).
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Biophysics 2021Quote: The ability of SHP2 to dephosphorylate the phosphopeptides was evaluated through a malachite green phosphatase assay (PTP assay kit 1 Millipore, MA, USA). The BTAM peptide and the following monophosphorylated peptides derived from known SHP2 substrates were used for comparison ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from leaves and roots of sunflowers from the genotype HA412-HO using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). Single strand copy DNA (ss-cDNA ...
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bioRxiv - Pathology 2020Quote: ... Eleven markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 11-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... Cells were allowed to adhere overnight at 26°C prior to being assessed for β-galactosidase activity associated with cellular senescence using a commercially available Senescence Cells Histochemical Staining Kit (Sigma Aldrich). The manufacturer’s protocol was followed with the following modifications ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from immature seeds of Trombay wild and TU94-2 plants with the help of Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse-I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Systems Biology 2020Quote: Plasma aliquots of blood collected from the tail vein after 4-hour fasting were used to measure the concentration of glucose and triglycerides by enzymatic colorimetric assays using commercially available kits (Sigma-Aldrich, USA) according to manufacturer’s instruction [17] ...
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bioRxiv - Neuroscience 2021Quote: ... PDMS with the standard 1:10 weight ratio of crosslinker to base (SYLGARD 184 Silicone Elastomer Kit, Dow Chemical) was diluted with hexane (Sigma-Aldrich, 34859) in a volume ratio of 1:9 ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50 μg lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Physiology 2020Quote: ... Secretion of glycerol and non-esterified fatty acid from cultured adipocytes into HBSS was determined with assay kits from Sigma-Aldrich (FG0100) and FUJIFILM Wako Diagnostics-NEFA Reagent (NEFA-HR(2)) ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Cell Biology 2019Quote: PLA was performed according to the manufacturer’s instructions using the Duolink In Situ Orange Kit Goat/Rabbit (Sigma Aldrich, Cat. No. DUO92106). Immunofluorescence was performed as previously described (Arno et al. ...
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bioRxiv - Biochemistry 2019Quote: ... β-hydroxybutyrate (HBA) was determined by coupled spectrophotomet-ric assay with β-hydroxybutyrate dehydrogenase (obtained as kit from Sigma, St. Louis, USA). It was verified that urine does not interfere with this kit ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The intracellular ATP content in the HGs was measured with an Adenosine-5’-triphosphate bioluminescent assay kit (FL-AA, Sigma Aldrich Inc.). Bioluminescent luciferase-luciferin reactions provide the basis of simple ...
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ethanol 70% (vol/vol) was added and the contents were placed in columns (GenElute® mammalian total RNA miniprep kit, Sigma Aldrich). The RNA was extracted according to the manufacturer recommendations ...
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bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
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bioRxiv - Physiology 2021Quote: ... A 30-μL portion of supernatant was used to measure circulating glucose levels with glucose oxidase assay kit (Sigma-Aldrich, GAGO-20) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Reduced and oxidized fractions of nicotinamide adenine dinucleotide phosphates (NADPH/NADP) were measured using the NADP/NADPH Quantitation kit (Sigma-Aldrich, USA) adhering to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Developmental Biology 2021Quote: ... Fluorescent membrane labelling of the VE was achieved using the CellVue Claret Far Red Fluorescent Cell Linker Kit (Sigma-Aldrich, MINICLARET-1KT). Briefly ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Immunology 2021Quote: ... Bulk endotoxin-free preparations of VIU-1005 and the empty control plasmid (control) were prepared for animal studies using GenElute™ HP Select Plasmid Gigaprep Kit (Sigma, Germany).
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA isolated from a 5-mL marine broth overnight culture was extracted with the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) and used as template ...
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bioRxiv - Immunology 2021Quote: The levels of cytokine and chemokines in BAL samples from NV1-treated and control SARS-CoV-2-infected mice were analyzed by Luminex multiplex assays using 36-plex MILLIPLEX® MAP Mouse Cytokine / Chemokine Magnetic Bead kit (EMD Millipore). BAL samples were quickly vortexed for 10s ...
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bioRxiv - Genomics 2021Quote: ... For the VPA treatment hiPSCs were differentiated to endoderm using a 2-day DE Induction Kit following the manufacturer’s instructions and from day 0 of differentiation 400uM VPA (valproic acid sodium salt 98%, Sigma Cat. # P4543-10G) in DMSO was added to cell cultures.
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bioRxiv - Microbiology 2020Quote: ... Templates used were either bacterial DNA extracted with the GenElute™ Bacterial Genomic DNA Kit or phage DNA extracted from phage lysates that had been precipitated as previously described and then extracted using the GenElute™ Bacterial Genomic DNA Kit with an extended proteinase K incubation of 1 hour (Sigma-Aldrich). 50 ng of template was used for each PCR ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the gDNA from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: We analyzed chemotherapy responses of human leukemia cell lines expressing wild-type NT5C2 or NT5C2 mutant constructs following 72-hour incubation with increasing concentrations of 6-mercaptopurine by measuring metabolic reduction of the tetrazolium salt MTT using Cell Proliferation Kit I (Sigma-Aldrich, #11465007001) following manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The column was calibrated using a gel filtration markers kit for protein molecular weights 29,000-700,000 Da (Sigma-Aldrich, product number MWGF1000).
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of ground sample was processed using the Spectrum™ plant total RNA kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... glycerol and triglyceride levels in each sample were measured using a Serum Triglyceride Determination Kit (Sigma-Aldrich, components F6428, T2449, and G7793) and normalized to protein levels (BCA Protein assay ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with propidium iodide and Hoechst 33342 from the Live-Dead Cell Viability Assay Kit (Millipore Sigma, Catalog CBA415) according to manufacturers’ instructions and immediately imaged at 10x magnification with a 0.17 numerical aperture CFI60 objective on a Keyence BZ-X710 microscope ...
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bioRxiv - Microbiology 2022Quote: Water samples collected from each site were vacuum filtered using the Millipore All-Glass Filter Holder kit (EMD Millipore Corporation, Billerica, MA). Collected water from each replicate was filtered through Whatman filter membrane to remove coarse to medium debris ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... The genomic DNAs were extracted from the clones with the GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO). The genome sequences were examined with PCR using the following primers ...
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bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and potential positive clones were grown overnight in LB media for plasmid production and further purification with GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). After confirmation of the insert sequence by sequencing (DNA Sequencing Unit ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-231-derived sEVs were labelled using the PKH67 Green Fluorescent Cell Linker kit (for general cell membrane labelling, Sigma-Aldrich, USA) according to manufacturer’s protocol ...