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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Four µl of the 40 μM Kif20A in BRB40 supplemented with 20 μM paclitaxel and 20 × 10−3 unit of potato apyrase (Sigma-Aldrich, MO), were then applied onto the EM grid and incubated for 1 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized in ice-cold methanol by dipping the coverslip in it 20 times followed by blocking in 3% BSA (Sigma-Aldrich; A9647) in PBS for 15 min (Figures 2 A-H and S1) ...
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bioRxiv - Cell Biology 2022Quote: ... mounted on an internal pressure micro kit extruder (SPG Technology Co.) and immersed into an oil phase (∼125 mL) consisting of hexanes (99%) and 3% (v/v) Span 80 (Sigma-Aldrich, S6760). 10 mL of gel mixture was extruded into the oil phase under nitrogen pressure of approximately 7 kPa ...
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bioRxiv - Developmental Biology 2022Quote: ... The surface on which the PAA gels were polymerized was activated by immersion into a solution of 3-methacryloxypropyltrimethoxysilane (0.3%, Bind-Silane, Sigma, cat. no. 440159), 10% acetic acid aqueous solution (3% ...
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bioRxiv - Cell Biology 2022Quote: ... eggs were harvested from the ampulla region of the oviducts in MEM containing 3 mg/ml of hyaluronidase (Sigma Aldrich, Cat# H3506) to aid detachment of cumulus cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections of the paraffin-embedded embryos (3-5 µm) were attached to poly-L-lysine-coated glass slides (Sigma, St. Louis, Mo) dried at 55 °C for 1 h and de-waxed twice in xylene (10 min each) ...
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bioRxiv - Genomics 2022Quote: ... Auxin induced degradation of RAD21-AID-mClover in the HCT116 + Rad21-mAC cells was performed by adding 500uM 3-Indoleacetic acid (IAA, auxin) (Sigma, 45533-250MG) dissolved in 100% Ethanol ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... German glass 18 mm #1 coverslips (Electron Microscopy Services, 72292-09) placed in a 12-well dish were washed in 3 M sodium hydroxide (Millipore Sigma, 221465) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Cancer Biology 2023Quote: ... PPAT (250mg) were saturated and permeabilized with PBS supplemented with 3 % BSA and 0.2 % Triton X100 (Sigma-Aldrich, Saint-Quentin-Fallavier, France) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Cancer Biology 2023Quote: ... then permeabilized in 200 μl of PBS1X supplemented with 3% fetal bovine serum and 0.1% saponin (Sigma-aldrich, Saint-Louis, MO, USA) for 5 min at room temperature (20–25 °C) ...
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bioRxiv - Genomics 2023Quote: ... Sections were rinsed in Phosphate Buffered Saline (PBS) and blocked in a humidity chamber in 3% normal donkey serum (Sigma-Aldrich, 566460) and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Blots were washed again 3 times for 10 min each and developed using Immobilon Luminata Crescendo Western HRP substrate (Millipore, Cat#WBLUR0500). Western blots were imaged and quantified using ImageLab (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... were transferred to 35 mm diameter petri dishes containing 3 ml of liquid GM supplemented with 10 μM estradiol (β-Estradiol, E8875, Sigma Aldrich, freshly prepared by diluting a 1 mM stock solution in DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed with the isopropanol (IPA) for 3 hours then rehydrated in DI and treated with 50U/ml DNase (Millipore Sigma, #10104159001) for 8 hours followed by an overnight DI rinse ...
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bioRxiv - Physiology 2023Quote: ... Mice were anesthetized with 3% isoflurane and an osmotic minipump (Alzet model 2004, #0000298) set to deliver (±)-isoproterenol hydrochloride (Sigma-Aldrich #I5627) dissolved in sterile saline at a dose of 70mg/kg/day for 2 weeks was inserted subcutaneously via a small midline incision in the back ...
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bioRxiv - Bioengineering 2023Quote: ... sections were stained with Oil Red O (3 mg/mL Oil Red O in 60% isopropanol; Sigma-Aldrich, St. Louis, MO, USA) for 6 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was changed to 2i + LIF medium consisting of base medium supplemented with CHIR99021 (Chiron, 3 µM, Sigma-Aldrich SML1046-5MG), PD0325901 (1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Plant Biology 2024Quote: ... Fungal colonization was quantified using internal transcribed spacer (ITS) primers (see Supplemental Table 3) and SYBR Green JumpStart Taq ReadyMix (Sigma Aldrich, 1003444642) with a QuantStudio5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... or 30 minutes (organoids and intestine-on-chip systems) at room temperature and blocked by incubation in bovine serum albumin (BSA, 3% weight/vol, Sigma Aldrich #A2153) diluted in DPBS for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... cumulus cells were dispersed by 3 to 5 minutes of incubation in M2 medium containing 350 IU/mL of hyaluronidase (Sigma-Aldrich H4272). After washing ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were visualized by Coomassie-stained SDS-PAGE and concentrated using a 3 kDa MWCO Amicon Ultra centrifugal filter (Millipore Sigma, #UFC9003,).
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bioRxiv - Microbiology 2024Quote: ... Cells were then blocked in 3% BSA before adding primary antibody mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend antibody (EMD Millipore, MAB8251). The primary antibody was followed by a secondary anti-mouse Alexa-488 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... isyn cells were transduced with a lentiviral Rab27b-targeted shRNA (5′-CCCAAATTCATCACTACAGTA-3′) to knock down Rab27b or with a lentiviral nontarget (nt) shRNA (Sigma-Aldrich SHC016), as a control as previously described [32].
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bioRxiv - Cell Biology 2024Quote: ... oocytes were isolated from the cumulus-oocyte complex by treating with 3 µg/ml hyaluronidase (H3506; Sigma-Aldrich, St Louis, MO, USA) for approximately 5 min.
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bioRxiv - Plant Biology 2024Quote: Gradient tB solution.30%, 50% and 70% Tert-Butanol (tB, Aladdin T119717) in water v/v and 3% w/v Quadrol (Sigma-Aldrich 122262) was added afterwards.
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bioRxiv - Biophysics 2024Quote: ... All solutions were prepared with ultrapure water (18.2 MΩ cm of resistivity) from a Milli-Q Integral 3 purification system (Millipore, Bedford, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... lysing the host cells via syringing with 20 and 25-gauge needles and filtering through a 3 μm Isopore™ membrane filter (Millipore; # TSTP02500). 3.5×105 parasites from each sample were then added into a T175 flask containing confluent HFFs in D10 medium ...
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bioRxiv - Plant Biology 2024Quote: AM 810–813 plasmids (Table S2) encoding active and proteolytically dead (PD) GST-tagged AtATG4A and AtATG4B were used to transform Rosetta (DE3) cells (Novagen, 70954-3). Expression was induced overnight at 16°C using 0.25 mM IPTG (Hellobio ...
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bioRxiv - Immunology 2024Quote: ... AAS99264.1 and AAO88201.1 respectively) and divided into 3 pools (15-mers overlapping by 10 aa, Sigma-Aldrich, United States or Mimotopes, Australia). For each serotype ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dishes were washed 3 times in phosphate buffered saline (PBS) for 5 minutes each then permeabilized with 0.1% Triton X-100 (Sigma, St. Louis, MO) in PBS for 40 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... Protein was purified by Nickel-NTA resin and treated with TEV protease (1mg lab made TEV per 40mg of protein) and benzonase-100units/ml (Novagen, 70664-3) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were suspended in 75 µL High Salt buffer (3 M sodium chloride, NaCl, Fisher Scientific; 25 mM dithiothreitol, DTT, Sigma; 25 mM ammonium bicarbonate, AB, Sigma Aldrich; protease and phosphatase inhibitor cocktail in accordance with the manufacturer’s guidance ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... dams received ‘Nilla wafer cookies treated with either vehicle (Veh; 3% DMSO in sesame oil, Sigma Aldrich, Cat. # D4540 and S3547, respectively) or the PCB mixture Aroclor 1221 (A1221 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were centrifuged (12,000 g for 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... BrdU was dissolved in 0.9% NaCl and injected at a concentration of 100 mg/kg 3 times each 2h (Sigma-Aldrich, Buchs, Switzerland). CldU was dissolved in 0.9% NaCl and injected at a concentration of 42.5 mg/kg either 3 times each 9h for the experiment about quiescence exit in vivo ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Neuroscience 2024Quote: ... To prevent movements and EMG contamination of the phrenic neurogram pancuronium bromide was administered (3 mg/kg IV, Sigma-Aldrich, St Louis) to achieve neuromuscular blockade ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then washed for 3 x 5-minutes washes in Duolink Wash Buffer A and then incubated in 40 μL of polymerase (Sigma Aldrich, DUO82028) diluted 1:80 in 1X amplification buffer (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed for 3 × 5 minutes in Duolink Wash Buffer A and then incubated in 40 μL of ligase (Sigma Aldrich, DUO82027) diluted in 1X ligation buffer (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... NPC-line 3: NPCs derived using the protocol of Shi et al., (Shi et al., 2012) from hiPSC line IPSC0028 (Sigma-Aldrich, RRID:CVCL_EE38). Line 1 NPCs were generated as previously described from hiPSCs grown on a mouse embryonic fibroblast (MEF ...
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-17A were assessed using the High Sensitivity T-cell Discovery Array 3-Plex (Millipore, St. Charles, MO, USA) at Eve Technologies using the Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... One day prior to each imaging session, 300 μL methoxy-XO4 solution (Glixx lab) (∼ 5 mg/kg, dissolved in 3% Cremophor®EL (Sigma) and phosphate-buffered saline ...
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bioRxiv - Immunology 2024Quote: ... Peritoneal macrophages (PerMacs) were collected from the peritoneal cavity via gavage 4 days after intraperitoneal injection of 3% brewer thioglycolate medium (Sigma-Aldrich, B2551) and plated in in medium with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected after centrifugation at 15,000×g for 30 min and DNA/RNA in the suspension was digested with 0.15 U/μl benzonase (Novagen, 70-746-3) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP was performed on 280-300ul sonicated material (∼25 µg of chromatin per sample) with 3 µg of rat anti-RNAPII-S2 antibody (Millipore, clone 3E10) by overnight incubation at 4 °C ...