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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Auxin-inducible degradation of proteins was induced by treatment with 0.5 mM indole-3-acetic acid (IAA) (Sigma, I2886-5G, CAS: 87-51-4) and 4 μM phytic acid dipotassium salt (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... After washing the fixed cells 3 times with PBS they were stained with Hoechst 33342 (2.5 µg/mL) (Sigma Aldrich, St. Louis, MO, USA) for 20 min and subsequently washed again twice with PBS ...
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bioRxiv - Immunology 2024Quote: ... the allosteric FFA2R modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide) and adenosine 5′-thriphosphate disodium salt hydrate (ATP) were from Sigma-Aldrich (Merck, Burlington, MA, USA). The FFA2R antagonist CATPB ((S)-3-(2-(3-Chlorophenyl)acetamido)-4-(4-(trifluoromethyl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mice were either euthanized or given doses of oral ciprofloxacin (3 mg per mouse; ciprofloxacin hydrochloride monohydrate, Sigma-Aldrich, dissolved in 100 μl sterile dH2O) for three consecutive days ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary and secondary antibodies used for detecting the 3×Flag were monoclonal ANTI-FLAG® M2 antibody produced in mouse (Sigma-Aldrich, Cat NO.: F3165) and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: K-562 cells were induced to differentiate into megakaryocytes at a density of 3 x 105 cells per ml using 40 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) and collected at the indicated time points after induction ...
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bioRxiv - Pathology 2021Quote: ALI cultures were fixed for 15 minutes on day 21-32 in 4% paraformaldehyde and stained for α-tubulin (Sigma-Aldrich, MAB1864; 3 μg/mL), MUC5AC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 plaque reduction assays were evaluated by first staining plaques with .05% Neutral Red solution for 3 hours at 37 °C (Sigma Aldrich cat. no. N2889-100mL). Neutral red was then aspirated from the wells and plaques were counted ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were blocked with the blocking buffer (3% bovine serum albumin [BSA, Sigma-Aldrich A3059] and 0.1% saponin [Sigma-Aldrich S4521] dissolved in DPBS) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Depletion of endogenous Spindly was achieved through reverse transfection with 50 nM Spindly siRNA (5′-GAAAGGGUCUCAAACUGAA-3′ obtained from Sigma-Aldrich, (Gassmann et al., 2010)) for 48 hours with RNAiMAX (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum, 0.09% sodium azide [Sigma-Aldrich, St. Louis, MO], 5mM EDTA), washed twice ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 5’-GTC GAC TTA GCT GTC CAT GTG CTG GCG TTC GAA TTT AGC GGT TTC TTT GAA TTC GGC AAA CTC CGT AAT AGT GG-3’ (Sigma-Aldrich Japan, Inc., Tokyo, Japan). The PCR products were cloned into a pCMV-Tag2 plasmid (Stratagene ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Systems Biology 2022Quote: ... was lyophilized and dissolved in 20 μL water containing two reference compounds (200 μM each of trimesate [Wako] and 3-aminopyrrolidine [Sigma-Aldrich, St. Louis, MO, USA]) for migration time and then injected into a capillary electrophoresis time-of-flight mass spectrometry system (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos older than 28 hpf were first anesthetized for 5–10 min in Ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, Saint Louis, MO, USA) in E3 medium ...
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bioRxiv - Microbiology 2020Quote: ... Colonies were suspended on sterile phosphate buffered saline (PBS) tube and the bacterial was vigorously vortexed for 10 min using 3-mm sterile glass beads (Sigma-Aldrich, Saint-Quentin-Fallavier, France) and passed three times through a 29 Gauges in order to eliminate bacterial aggregates ...
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bioRxiv - Cell Biology 2020Quote: ... pancreatic sections were incubated with 1X Phosphate-Buffered Saline with Ca2+ and Mg2+ (PBS 1X) supplemented with 3% H2O2 (cat. H1009 - Sigma Aldrich, St. Louis, MO, USA) for 30min ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed as before and placed in HBSS w/ Ca2+ and Mg2+ containing 1x Sybr® green (Sigma-Aldrich CAS: 163795-75-3). Cells were incubated at RT for 20 min in the dark and read immediately on a BD LSR Fortessa flow cytometer.
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bioRxiv - Cancer Biology 2023Quote: ... of HaCaT and OSCC (SAS, HSC-3, Ca9-22) cell lysates as well as FAD and NADH (both from Sigma-Aldrich, Missouri, USA, as controls) were measured using a fluorescence spectrophotometer ...
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bioRxiv - Cancer Biology 2023Quote: ... Tamoxifen induction was performed in mice at 3-5 months of age by oral delivery of tamoxifen (Sigma; 100 mg/kg/day in corn oil) for 4 consecutive days as described previously69 ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tomato plants were placed into 121 L transparent methacrylate chambers containing hydrophilic cotton swabs soaked with 5 μM of (Z)-3-hexenyl butyrate (HB; Sigma-Aldrich, Saint Louis, MO, USA) or distilled water in the case of control plants ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were trimmed down to a 3-5 mm region around the area of interest and embedded in 5% (w/v) agarose (A9539; Sigma-Aldrich, St. Louis, MO, USA) in deionized water ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% [v/v] Triton X–100) and incubated at 37°C for 3 h either with α–mouse plus and α–rabbit minus for PLA assay (Sigma–Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Physiology 2022Quote: ... The luciferase in solution was centrifuged at 4° C (14,000g) in filtered centrifugal tubes until the volume within the centrifugal tubes had reduced by 80 % (Amicon Ultra, Millipore, Ireland, molecular cut-off 3 kD). The filtrate was discarded and 0.5 M Trizma acetate solution was added to the luciferase solution at a volume equal to the volume of the discarded filtrate ...
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bioRxiv - Bioengineering 2024Quote: 3-(Trimethoxysilyl)propyl methacrylate (Cat. #M6514, lot #SHBG7600V, Sigma-Aldrich, Oakville, Ontario, Canada; fluorescein sodium salt (Cat. #46960, lot #2082530, Sigma-Aldrich, Oakville, Ontario, Canada), isopropyl alcohol (IPA ...
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bioRxiv - Cell Biology 2024Quote: ... skeletally matured 3-month-old female and male mice of all genotypes received an intraperitoneal injection of 100 mg/kg tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) dissolved in palm oil (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... with the exception of Akkermansia muciniphila DSMZ 22959 that was pre-cultured in M2-based medium supplemented with 3 g/L of type II mucin (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland), and Veillonella parvula DSMZ 2008 that was pre-cultured in M2-based medium supplemented with 60 mM of DL-lactic acid 90% (Sigma-Aldrich Chemie GmbH ...
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bioRxiv - Microbiology 2023Quote: ... Single colony was picked up into 50 ml 3-(N-morpholino) propane sulfonic acid medium (MOPS) buffered defined liquid medium (Sigma-Aldrich, St. Louis, MI, USA). For DSM1092 ...
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bioRxiv - Developmental Biology 2022Quote: NPCs cells were plated into Matrigel-coated 10mm2 plate and treated for 3 days with SAHA (20 nM, Sigma-Aldrich, in DMSO (0.05%, Sigma-Aldrich)) in culture media before performing western blot analysis ...
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bioRxiv - Neuroscience 2022Quote: ... we performed an additional DWI measurements by mimicking increasing ISF space volume in 3 water phantoms filled with Sephadex G-25 microbeads (Sephadex G-25; Sigma-Aldrich, St. Louis, MO, USA) of coarse (100–300 μm) ...
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bioRxiv - Plant Biology 2022Quote: ... homogenized in extraction buffer A (10mM Tris-HCl, 0.4M sucrose, 10mM MgCl2, 2mM PMSF, 5mM β-mercaptoethanol, 1µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3), incubated with gentle shaking for 20min ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants containing the cytosol of the cell pools were combined and concentrated in an Amicon® centrifuge concentrator tube (3 kDa cut off, Sigma Aldrich, Cat. No. UFC901024) by centrifugation at 4,000xg/4°C for 30-45 min until a volume of 1.5-2.0 ml was reached.
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bioRxiv - Neuroscience 2024Quote: ... brain sections were washed in phosphate buffer (PB) and incubated 1h in a solution containing equal volumes of 3% potassium ferrocyanide (Sigma-Aldrich, Ontario, Canada, cat# P9387) with 4% osmium tetroxide (EMS ...
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bioRxiv - Biochemistry 2024Quote: ... six whole blood units were processed as above and suspended in a custom AS-3 formulation containing 50%L1,2-13C2-Glucose (Sigma Aldrich, St. Louis, MO, United States). When calculating % 13C enrichment as a fraction of the total isotopologues ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465], 20% ethanol [Fisher BP2818100], 0.75% acetic acid [Millipore Sigma A6283], 5mM NaOH [Millipore Sigma 72068], in ddH2O) for 15 min at 4C ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently membranes were solubilized with CL-91 and processed as described above for APs with a mixture of anti-Nrx1-3 antibodies (anti-Nrx1, #ABN161, Millipore; anti-Nrx1, Synaptic Systems, #175103) and subjected to MS-analysis.
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bioRxiv - Microbiology 2022Quote: ... The paraformaldehyde was then removed and the cells were washed twice with 100 μl of 1XPBS and stained with100 μl of DAPI (Sigma Aldrich catalog no. 28718-90-3) working solution (600 nM DAPI ...
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bioRxiv - Immunology 2020Quote: ... A 3-fold serial dilution of the sera was performed and HRP goat anti-mouse γ-chain was used (SIGMA, Saint Louis, MO, United States A9044). The result was registered using a microtitration plate reader (Anthos 2010® ...
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bioRxiv - Immunology 2020Quote: ... After dialysis external solutions were mixed and were concentrated by Centricon YM-3 (from Merck-Millipore, used at 5000 x g for 30 minutes, 4 °C) to reach 1 ml as final volume ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue sections were then washed in 1x PBS (3×5 min) before incubation with donkey anti-chicken IgY 647 (Sigma-Aldrich, St. Louis, MO, United States) and donkey anti-mouse IgG 488 (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Physiology 2024Quote: ... The injected hormone solutions were prepared from crystallised powder (T3: CAS number 6893-02-3, Sigma-Aldrich, St. Louis, MO, USA and T4: CAS number 51-48-9, Sigma-Aldrich, St. Louis, MO, USA). The amount of hormone injected (T4 ...
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bioRxiv - Immunology 2024Quote: ... cells were either stimulated or not with 20 ng/mL of the γδ T cell stimulator (E)-4-Hydroxy-3-methyl-but-2enyl pyrophosphate (HMBPP; Sigma-Aldrich, Cat. No. 95098-1MG). CD107a-BV421 (Biolegend) ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed 3 times for 10 minutes each and incubated in the hydrogel solution containing 19% of sodium acrylate (Sigma 408220 or Pfaltz and Bauer SO3880), 10% acrylamide (Sigma A9099) ...
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bioRxiv - Neuroscience 2024Quote: ... mitoEGFP mice 3 to 5 months of age (mos) received single intraperitoneal injections of 1mg/kg of Lipopolysaccharide (LPS) (Millipore Sigma, strain O55:B5, L2880-25MG) or saline ...
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bioRxiv - Plant Biology 2024Quote: ... and 400 seeds from each pool were then grown for 4 days in the dark in liquid media after sterilization and stained with Ruthenium Red dye (Verger et al., 2016) Liquid media was removed and 3 ml of Ruthenium Red dye (Sigma Corporation, 0.5 mg/ml in dH2O) was applied to seedlings for 2 min in a 10 ml microtiter growth plate ...