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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... hearts were prepared for microscopic studies by immediate immersion into 3 M KCl to stop its function in diastole and placed into 20% sucrose (Sigma Aldrich) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... after which the rats in the ACTH group (n=50) were administered with either 3 injections of imipramine (n=10) (IMI, 15 mg/kg i.p. (Sigma-Aldrich, Denmark)) or 1 injection of saline ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was changed every 3–4 d and enriched with granulocyte-macrophage colony-stimulating factor (5 ng/ml GM-CSF, Sigma-Aldrich). After approximately 14 d at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: Three pairs of Day 56 forebrain organoids derived from iPSCs of three CTRL and WS patients were dissociated with 3 mL Tryp-LE (Sigma-Aldrich) for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The SE lipid mixture of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine): DPC (dihexadecyl phosphate, a negatively-charged lipid): CH (cholesterol) (catalog #s: Avantis Polar Lipid 850345P, Sigma Aldrich 2197-63-9 ...
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bioRxiv - Neuroscience 2024Quote: Custom 3D printed cavity was filled with fluorescein dye-infused with 3% agar gel (10% v/v, catalog no. F2456; Sigma Aldrich). The cranial implant was then placed within a mold made on the open surface of the cavity that leveled the top surface of the implant with the top surface of the cavity and filled up the entire bottom transparent PET window surface with fluorescent gel ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves were harvested and fixed for 1 h in 4% paraformaldehyde (PFA) followed by 15 min immersion in a 3% sodium fluorescein (NaFlu, molecular weight: 376 Da; Sigma Chemicals) in saline solution 2 ml of Evans blue albumin (EBA ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a series of washes in PBST (PBS with 3% Triton™ X-100, Sigma-Aldrich, X-100) (once for 10 minutes ...
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bioRxiv - Biophysics 2024Quote: Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate (cAMP) and guanosine 3’,5’-cyclic monophosphate sodium salt (cGMP) were purchased from Sigma Aldrich and both prepared at 16 mM in 150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... The duplex samples were then buffer exchanged into NMR buffer using Amicon Ultra-15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma) at least 3 times ...
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bioRxiv - Cell Biology 2024Quote: Cell differentiation was conducted using previously described protocol 32 with slight modifications by using sequential inhibition of GSK-3 by CHIR99021 (8 µM, Sigma-Aldrich) and WNT by IWP-2 (5 µM ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465], 20% ethanol [Fisher BP2818100], 0.75% acetic acid [Millipore Sigma A6283] ...
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bioRxiv - Microbiology 2024Quote: ... cells in each culture plate were transfected with 10µg of pNL4-3 plasmid DNA complexed with 50μL PEI reagent (Sigma-Aldrich catalog # 764604), and the culture media was changed 16 hours post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... The suspension was transferred to FACS tubes cells were washed twice with FACS-buffer (DPBSdef + 3 % foetal calf serum (Sigma-Aldrich) + 2 mM EDTA (Roth ...
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bioRxiv - Immunology 2024Quote: ... Viral transduction was performed by spinfection at 900 g at 25 °C for 3 h with 10 μg/ml polybrene (Sigma-Aldrich), followed by resting for 3 h at 37 °C in 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... the auxin-inducible degron was induced by adding 500 μM indole-3-acetic acid (IAA, chemical analog of auxin) (Sigma, I5148) to the media ...
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bioRxiv - Cell Biology 2024Quote: ... After rinses with 0.1 M PBS followed by dipping in Milli-Q water (DirectQ® 3 UV water purification system, Millipore, MA). Sections were air-dried and cover slipped with Prolong® diamond antifade mounting medium (Cat ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Molecular Biology 2024Quote: ... A total of 4×108 cells were first fixed with 3% pFA at the culture temperature for 10 minutes and subsequently with 10 mM dimethyl adipimidate (DMA; Sigma, 285625) at room temperature for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein knock-downs using the mAID system were performed under 500 µM auxin (IAA: 3-indoleacetic acid in 100% ethanol; Sigma-Aldrich) [64].
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bioRxiv - Molecular Biology 2024Quote: Embryos were anesthetized in 0.016% tricaine methane-sulfonate (Pentair, Apopka, FL) and mounted in 3% methylcellulose (Sigma-Aldrich, St. Louis, MO). Embryos were imaged using Leica Thunder Imager M205 FCA ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465] ...
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bioRxiv - Neuroscience 2024Quote: Incubation chambers were set up to submerge slices in oxygenated (95% O2, 5% CO2) aCSF containing a selection from the following drugs: 3 µM puromycin dihydrochloride (Sigma P8833), 100 µM dopamine hydrochloride (Sigma H8502) ...
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bioRxiv - Systems Biology 2024Quote: The filtrate was lyophilized and suspended in 50 μL of water containing the reference compounds [200 μM each of trimesate (206-03641, Wako) and 3-aminopyrrolidine (404624, Sigma-Aldrich)] ...
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bioRxiv - Cell Biology 2024Quote: ... the media were switched into Phase II erythroid differentiation media consisting of Stem Span SFEMII supplemented with 3% normal human serum (Sigma-Aldrich), 3 IU/ml erythropoietin (R&D Systems ...
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bioRxiv - Genetics 2024Quote: ... alkylated proteins were pre-digested for 3 hours at RT using 0.2 µg LysC after which the sample was diluted by adding 3 volumes of 50 mM ammoniumbicarbonate prior to overnight digestion with AspN at 37°C in 1mM methylamine (Sigma-Aldrich). Digests were diluted 1:1 with 2% trifluoroacetic acid and stored at -20°C prior to shotgun proteomics analysis.
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bioRxiv - Microbiology 2024Quote: All variants were prepared at a concentration of 3 µM with a final concentration of 10X SYPRO Orange Protein Gel Stain (Sigma-Aldrich) in a white ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Degradation of EGFP-AID-STAG2 was induced by the addition of S00 µM Inole-3-acetic acid (IAA, ISl48, Sigma-Aldrich) for 2 hours at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: CB6F1 mouse ovaries were removed from the bursa and placed in L15 medium supplemented with 3 mg/ml polyvinylpyrrolidone (PVP, P0930, Sigma-Aldrich) and 0.5% Pen-Strep ...
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bioRxiv - Synthetic Biology 2024Quote: ... The strain was transformed with a plasmid containing a 3-methyl benzoate (3MB; m-Toluic Acid; Sigma Aldrich Catalog No. T36609) inducible recT recombinase and mutLE36K (pTE583 ...
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bioRxiv - Pathology 2024Quote: ... Slides were then briefly submerged in acid alcohol (300 ml dH20, 700 ml 100% ethanol, 3 ml concentrated HCL; Sigma-Aldrich), followed by tap water wash ...
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bioRxiv - Systems Biology 2024Quote: ... morphine paradigm were communally housed (3-4 mice per cage) and had access only to water supplemented with either sodium butyrate (Sigma-Aldrich) or pH-matched (9.30 - 9.50 ...
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bioRxiv - Molecular Biology 2024Quote: ... N-(4-ethylphenyl)-2-[[4-ethyl-5-(3-pyridinyl)-4H-1,2,4-triazol-3-yl]thio}acetamide (ORco Receptor Agonist ORcoRAM2; OA) from Asinex Corporation and Vitas M Chemical Ltd; N,N-diethyl-3-methylbenzamide (DEET; V) from Sigma-Aldrich; and coelenterazine from Biosynth ...
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bioRxiv - Neuroscience 2024Quote: ... newly enclosed flies were maintained in vials with standard cornmeal food for 3 days and transferred to standard cornmeal food supplemented with 400 μM all-trans-retinal (Sigma R2500) for 2 days before experiments ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the PG sample was digested for 3 h at 37°C with 4 µg of lysozyme (Sigma-Aldrich) and then ...
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bioRxiv - Microbiology 2024Quote: ... Cells yielding in a total OD600 of 3 were pelleted and resuspended in 80 µl of PBS and 20 µl of 10 % Sarcosyl (Sigma Alderich). Then ...
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bioRxiv - Microbiology 2024Quote: ... The bone marrow-depleted ankles were cut into 3-4 mm sized tissue pieces and incubated with digestion media containing 2.4 mg/ml hyaluronidase (Sigma-Aldrich, #H3506), 1 mg/ml collagenase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: Harvested supernatants were passed through a 0.22 μm filter followed by concentration on a 3 kDa molecular weight cutoff Centricon Plus-70 filter (Millipore Sigma UFC700308). Samples were then precipitated with TCA (10% final concentration ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 3 % BSA for 20 min and incubated with 4 µg anti-FLAG monoclonal antibody (Sigma SAB4200119) for 20 min at room temperature ...
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bioRxiv - Genomics 2024Quote: ... HCT116-POLR1A-AID cells were treated with 500 μM Indole-3-acetic acid sodium salt (IAA, Sigma Aldrich, #6505-45-9) to degrade endogenous POLR1A ...
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bioRxiv - Neuroscience 2024Quote: ... 5 nmol of 5’/3’ diamino-modified ssLNA (IDT) was mixed 100 nmol of NHS-Alexa Fluor 568 or NHS-AlexaFluor 647 (Sigma Aldrich) in 1:1 PBS:DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenized tissue was centrifuged at 16,000 g for 45 min at 4 °C and the remaining supernatant was spun in 3 kDa cut-off Amicon® centrifugal filter tubes (Merck Millipore) at 14,000 g for 90 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... After homogenizing the tissue in ice-cold PBS with protease inhibitors (1mM PMSF; Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich; Complete, Roche), the extracts were crosslinked with 1% formaldehyde for 10 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating VTA brain sections were incubated for 3 hours at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Cancer Biology 2024Quote: Cells were detached the day following transfection with 0.25% trypsin–EDTA (Wisent) and plated at a density of 3 × 104 cells/well in a poly-l-ornithine (Sigma-Aldrich)-coated 96-well white bottom plate (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM glucose final concentration; 10 g tryptone [Fisher Bioreagents BP1421], 5 g yeast extract [Fisher Bioreagents BP1422], 3 g K2HPO4 [Sigma-Aldrich P3786] ...
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bioRxiv - Immunology 2024Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were permeabilised in 0.1% TritonX100 PBS with 3 times 15-minute wash and were blocked in 10% anti-donkey serum (Sigma, Cat# D9663) in 0.1% Triton X100 PBS for at least 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were suspended in 75 µL High Salt buffer (3 M sodium chloride, NaCl, Fisher Scientific; 25 mM dithiothreitol, DTT, Sigma; 25 mM ammonium bicarbonate ...