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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Trop2 was knocked out in mouse mammary tumor cell lines 4T1 (MGH Center for Molecular Therapeutics Cell Bank) and AT-3 (Sigma-Aldrich, SCC178) using TROP2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (ABM ...
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bioRxiv - Plant Biology 2024Quote: ... to calculate the volume equivalent of 100 µg of protein before loading onto a 3 kDa molecular weight cutoff (MWCO) filter (Merck Millipore, Bayswater, Australia). Tryptic peptides were prepared using the FASP protocol as previously described (75) ...
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bioRxiv - Biochemistry 2024Quote: ... High molecular weight components were separated from peptides by centrifugal ultrafiltration using a molecular weight cut-off 3 kDa (Centricon, Millipore, Schwalbach, Germany). The filtrates were fractionated on a Smart HPLC system (Amersham Biosciences ...
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bioRxiv - Genomics 2024Quote: The genomic DNA band from the CHEF gel was excised and placed inside a D-Tube Dialyzer (Millipore, CAT#71508-3, USA). The D-Tube was filled with a 0.5×TBE buffer and placed in the middle of the chamber of the Mapper XA system subjected to switching intervals of 1,200-3,600 seconds for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The chips were previously sterilized by UV irradiation for a period of 3 hours and coated with a solution of 0.0005% fibronectin (#ref: F1141, Sigma-Aldrich, Missouri, USA) and 0.02% gelatin (#ref ...
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bioRxiv - Biochemistry 2024Quote: Lipids were extracted and alkyne-modified lipids were reacted with 3-azido-7-hydoxycoumarin (Cat. No. 909513, Sigma-Aldrich, Oakville, ON, CA) and resolved on a TLC plate as previously described (50) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed 3 times for 10 minutes each on 0.1% v/v Triton X-100/DPBS and mounted with Fluoromount (Sigma-Aldrich catalog #F4680). Images were acquired with 63× objective 1.35 N.A ...
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bioRxiv - Cancer Biology 2024Quote: ... Buffer on top of cells was collected and further incubated with 3 mM 4-nitrophenyl-N-acetyl-β-D- glucosaminide substrate (Sigma, N9376) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples used in the biophysical and biochemical studies were prepared in indicated buffers as needed via buffer exchange using a 3 kD cutoff filter (EMD Millipore, UFC503024). The SERF2 protein concentration was determined using a Pierce TM BCA assay calibrated with the SERF2 A51W mutant serving as a standard ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each well of the next 3 rows was plated on SCM-Arg with canavanine (60 mg/L canavanine, Sigma Aldrich, Table S3), and colonies were counted after 3 days of incubation (30°C) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for a minimum of overnight and up to three days at 4°C in a blocking buffer of filtered 3% bovine serum albumin (Sigma A9418- 50G), 0.02% Tween-20 (Thermo 28320) ...
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bioRxiv - Genetics 2024Quote: All zebrafish larvae used for measurements or image collection at 48 hpf or later were first anesthetized using ethyl-3-aminobenzoate methanesulfonate salt (Tricane/MS-222; Sigma Aldrich #E10521) prepared at a concentration of 4 mg/mL using 1 M Tris (pH 9 ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were permeabilized with 1X PBS with 0.5% Triton X-100 and then placed in blocking solution (3% BSA (Sigma-Aldrich; 05470-5G) in 1X PBS with 0.2% Triton X-100 (Sigma-Aldrich X100-5ML) ...
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bioRxiv - Developmental Biology 2024Quote: ... E10.5 embryos were incubated in 4-nitro blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP; Sigma, 5655-25TAB) except when detecting Six1 and Mcrs1 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were diluted to 2 tubes of 0.5 mL of 2 mg/mL of lysate and treated each with 55 μL of Biotin Click master mix comprised of 30 μl of 1.7 Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine (Sigma) in 4:1 tBuOH ...
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bioRxiv - Biochemistry 2023Quote: ... Click Chemistry Master Mix was comprised of 6 μl of 1.7 Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine (Sigma) in 4:1 tBuOH:DMSO solution ...
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bioRxiv - Cell Biology 2024Quote: ... Click Chemistry Master Mix was comprised of 6 µl of 1.7 Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine (Sigma) in 4:1 tBuOH:DMSO solution ...
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bioRxiv - Pathology 2021Quote: The effect of SARS-CoV2 infection on cell viability of hMADS adipocytes was measured using the metabolic dye [4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium (MTT) (Sigma, St. Louis, MO, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Microbiology 2020Quote: ... 29 The sample was dried under nitrogen gas flow and purified with a 3 kDa Amicon Ultra centrifugal filter (Millipore, Temecula, CA, USA) to remove low molecular weight impurities ...
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bioRxiv - Cell Biology 2020Quote: ... transfected cell lines were grown on 22×22 mm coverslips and then fixed as follows: 3-5 minutes in cytoskeletal buffer (Byron et al., 2013) plus 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO) and 10 mM ribonucleoside vanadyl complex (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Alginate lyase was removed from culture medium prior to assay by filtering the medium through 3 kDa Amicon Ultra molecular weight cutoff filters (MilliPore Sigma, Burlington, MA). To maintain plasmids in 12B01,12.5 µg/mL chloramphenicol was added to the medium.
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bioRxiv - Developmental Biology 2021Quote: ... The nuclear pellet was resuspended in 250 μl of cold PBS and incubated at room temperature with 15 μl RNase A (Millipore Sigma 70856-3) and 10 μl RNase H (New England Biolabs M0297S) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we precipitated the cells by centrifugation at 13,000 g for 3 min and rinsed them with sterilized MilliQ water (Millipore Corp., Burlington, MA, USA) twice ...
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: Samples were incubated with primary antibody overnight at 4°C in PBT (PBS with 3% Triton X-100 (Sigma-Aldrich X100-100 mL)) with 5% NGS (Capralogics GS0250) ...
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bioRxiv - Molecular Biology 2020Quote: ... Up to 180mL of blood was collected into 3 60 mL syringes pre-loaded with 6 mL of anticoagulant acid citrate dextrose (ACD) (Sigma C3821 Lot#SLBW6172). Within 15 min of collection ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 3 times over 45 mins before fixation in phosphate-buffered saline (PBS) containing 4 % paraformaldehyde and 4 % sucrose (PFA, Sigma-Aldrich, Cat.# P6148) at 4 °C overnight ...
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bioRxiv - Biophysics 2021Quote: ... in a 24-well (Starlab) in neuro basal medium supplemented with B27 (Gibco, Cat: 17504001, 25 × 10−3 М KCl (Sigma, Cat: P9541), 1× 10−3 М glutamine (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and C-terminal (YAPGTGGGATI-NH2, 1.5 mg) regions of proGATI were individually conjugated to keyhole limpet hemocyanin (KLH; 3 mg; Sigma H-7017, Lot 110K4833). An additional Cys residue added to the N-ter peptide allowed conjugation to KLH using m-maleimidobenzoyl-N-hydroxysuccinimide ester ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Biochemistry 2021Quote: ... were combined and concentrated to approximately 150 µL in centrifugal filter units with a 3 kDa molecular mass cutoff (Amicon ultra centrifugal filters, 0.5 ml, Millipore, centrifugation at 14,000 g).
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bioRxiv - Systems Biology 2021Quote: ... 3 replicates of each cell line were grown for 48h in either growth medium with either [U-13C] glucose (Sigma-Aldrich, cat.no. 389374), [U-13C] glutamine (Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... Each bag was injected with 20 mL of Carbon-13C dioxide (99 atom % 13C, <3 atom % 18O, Sigma Aldrich, (St. Louis, MO, USA)) for a ratio of 10 mL of 13C·L−1 of air and was left undisturbed for 2 hours ...
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bioRxiv - Plant Biology 2020Quote: Newly emerged leaves were sampled from the F2 parents of the F2:3 mapping population and desiccated using silica gel (Millipore Sigma, Burlington, MA). Total genomic DNA was then isolated using the DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times with 1X PBS prior to blocking with 3% bovine serum albumin (BSA; Sigma #A7906, Saint Louis, MO) in 1X PBS ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Microbiology 2021Quote: ... Organoid sections were air dried and washed three times for 10 minutes at RT followed by blocking and permeabilizing for 60 mins with a solution containing 3% bovine serum albumin (Sigma, Cat. A9418-50G) and 0.1% Triton X-100 in 1XPBS ...
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bioRxiv - Microbiology 2020Quote: ... and 3-amino-9-ethylcarbazole (AEC) solution (Zytomed GmbH, Berlin, Germany. IHC-stained slides were counterstained with Hematoxylin solution (Sigma-Aldrich, MO, USA). Stained samples were mounted using Aquatex mounting medium (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Melan-ink4a cells were plated at 8.5 × 104 cells/well in 12-well plates and transduced on the next day with approximately 3 × 105 plaque-forming unit (PFU) of lentiviral particles in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich, Steinheim, Germany). After 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... in water for injection containing 3% sterile-filtered sucrose using a 0.22-μm Millipak®-200 polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA) [11].
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bioRxiv - Cell Biology 2022Quote: ... Lipid fluorophore 1,2 – Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647 (Atto 647 DOPE) was purchased from Sigma Aldrich (St. Louis, MO). HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Sigma Aldrich, Taufkirchen, Germany) for 5-30 min.
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bioRxiv - Biochemistry 2022Quote: ... followed by concentration and removal of excess linker using 3 kDa molecular weight spin filters as per manufacturer’s instructions (Amicon, Millipore Sigma, St. Louis, MO). To 400 µL of recovered GAG-amine was added 200 µL of 100 mM sodium phosphate ...
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bioRxiv - Cell Biology 2022Quote: ... this diluted solution was loaded onto an 18 mm round coverslip (Marienfeld precision cover glass thickness No. 1.5H) which has been sequentially coated with 3 µm microbeads (Sigma Aldrich, 79166-5ML-F) and poly-D-lysine (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Transfected cells were supplemented three days after transfection with 3 g/L glucose (Bioconcept) and 5 g/L soy hydrolysate (Sigma-Aldrich Chemie GmbH). Cell culture supernatant (423 mL ...
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bioRxiv - Neuroscience 2020Quote: ... On the day of the experiment the cells were placed on ice and washed 3 times with PBS supplemented with 10mM EDTA (Sigma, 150-38-9) for 3 minutes each ...
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bioRxiv - Biophysics 2021Quote: Methanol-fixed infected cells and a methanol-fixed negative control were incubated overnight at 4 C° with 150 μl of a solution of anti-S IgG or anti-N IgG in PBS + 3% BSA (Sigma-Aldrich, Milan, Italy). For colocalization experiments ...
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bioRxiv - Biochemistry 2021Quote: ... Chemotherapeutic drug-treated cells were incubated with 10 μL 3-(4,5)-dimethylthiazol (-z-y1)-3,5-diphenyltetrazolium bromide (MTT) solution (5 mg/mL, Sigma-Aldrich, St Louis, MO, USA) for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads were washed 3 times with lysis buffer and TFEB or TFE3 eluted using 200 ng/μL 3X-flag-peptide (Sigma-Aldrich, F4799-4MG).