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Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... For chromatin immunoprecipitation experiments cells were also treated with RXR agonist LG100268 (LG) (100 nM, Sigma Cat# SML0279, CAS:153559-76-3). For western blotting experiments cells were treated with LXR ligands for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The RSCs from both mice were placed together and the tissue was cut into small pieces and transferred into a 1.5 ml microcentrifuge tube with 3 mg/ml isolation solution containing pronase (Sigma, Cat#P6911-1G), 1% BSA and 50 μg/ml DNaseI (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: To induce the degradation of mAID-fused proteins, cells were treated with 500 µM indole-3-acetic acid (auxin, IAA, dissolved 500 mM in water) (Millipore Sigma, I5148) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared for NMR by combining 16 µL standard with 50 µL serum and placing in a 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... Individuals were euthanized with a 0.1% solution of ethyl 3-aminobenzoate methane sulfonate (MS-222; Sigma, St. Louis, MO, USA; A-5040). The brains were removed and fixed by immersion in 4% w/v paraformaldehyde in 0.1 M phosphate buffer (PB ...
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bioRxiv - Bioengineering 2021Quote: ... Devices were first exposed to air plasma (50 W) followed by filling channels with an aqueous solution of (3-Aminopropyl)triethoxysilane (APTES) (Sigma Aldrich, Germany). Devices were then incubated for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at a concentration of 1.5 mM was sonicated in nanopore water with 3 mM lactic acid (Sigma-Aldrich, L1750, St. Louis, MO) and sterilized via passage through a 0.2 μm filter as described in (5) ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Neuroscience 2021Quote: ... Tissue from three rats per sex was combined and transferred to 5 ml of ice cold lysis buffer (10 mM tris-HCl, 10 mM NaCl, 3 mM MgCl2 and 0.1% Igepal in nuclease-free water (Sigma-Aldrich, 18896-50ML) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Biophysics 2020Quote: ... An emulsion was generated by injecting 50 μL of pre-bead-solution in 500 μL of n-hexane with 3% Span®80 (Sigma-Aldrich) using a 100 μL Hamilton Syringe ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared by layering supernatant gently on top of a discontinuous Percoll-density gradient (3% uppermost, 10% middle, and 23% bottom; Percoll, P1644, Sigma-Aldrich, UK) and centrifuged at 47,807 g for 8 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The surfaces were rendered hydrophilic or hydrophobic by vapor phase functionalization using 3-aminopropyltrimethoxy silane or chlorotrimethylsilane (Sigma Aldrich, St. Louis, MO), respectively ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were harvested during the exponential growth phase by centrifugation for 10 min at 10,000 g at room temperature with a Sigma 3-18K centrifuge (Sigma Aldrich, Seelze, Germany), washed with the same volume of fresh medium (without glucose or thiamine) ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cell Biology 2021Quote: ... and immersed into the oil phase (~125 mL) consisting of hexanes (99%) and 3% (v/v) Span 80 (Fluka, 85548 or Sigma-Aldrich, S6760). 10 mL of gel mixture was extruded through SPG membranes under nitrogen pressure of approximately 7 kPa ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... one artificial plant (AP) having five leaves made of a Whatman filter paper (grade 237 equivalent to grade 3 CHR; Sigma-Aldrich, India) was also used as an inert control ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed by sterile phosphate buffer saline (PBS) 3 times and then incubated with 5 mg/mL MTT (Sigma-Aldrich; #M2128) for 4 h ...
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bioRxiv - Molecular Biology 2022Quote: ... and samples were incubated in blocking solution (3% powdered nonfat milk in PBS + 0.1% IGEPAL CA-630 (Sigma-Aldrich, 18896, lot 1043) for 10 minutes minimum at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or 3-week old mice (P21) were dissected to remove the skin and fat and stained with Alizarin Red (Sigma-Aldrich A5533) for visualization of the mineralized elements and Alcian Blue (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... was amplified from pBluescript II with SalI adapters using primers KL215 and KL216 and KOD Hot Start DNA polymerase (Millipore 71842-3). The PCR product was purified using a QIAquick PCR Purification Kit (Qiagen 28104) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices with Cav2-Cre injection at MPC and lENT were immunostained by Rabbit anti-Cre antibody (Millipore Sigma, Cat#69050-3) and Donkey anti-Rabbit secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... DS pupae were injected with either 3 μl of 2000 pg/μl (6000 pg total) (10% 20E in EtOH + 90% saline) of 20E (Sigma-Aldrich®) or with 3 μl of vehicle (10% EtOH and 90% saline ...
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bioRxiv - Immunology 2020Quote: ... and 24S-25,epoxycholesterol (10 µM, Enzo Cat# BML-GR231-0001, CAS: 77058-74-3) were compared to vehicle (ethanol, Sigma Cat# E7023). The UGCG inhibitor N-Butyldeoxynojirimycin (NB-DNJ ...
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bioRxiv - Bioengineering 2021Quote: ... Devices were first exposed to air plasma (50 W) and followed by filling channels with an aqueous solution of (3-Aminopropyl)triethoxysilane (APTES) (Sigma Aldrich, Germany). Devices were then incubated for 1 minute at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
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bioRxiv - Cell Biology 2020Quote: ... cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma; polymerization for 3 days at 60°C). Firstly ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... incubated for ~3 h at room temperature and finally the wells were thoroughly rinsed with phosphate-buffered saline (PBS; Sigma Aldrich, UK). The coverslips remained functional for at least two weeks when stored at 4°C in plastic pipette tip box containing a layer of deionised water at the bottom ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Systems Biology 2020Quote: ... we split and seeded iPS-GM942-SeV3 into Geltrex-coated 12-well plates at a density of 3 × 105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). After 24 hours ...
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bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing α-synuclein confirmed by Coomassie-stained SDS-PAGE were dialyzed into PBS and concentrated with an Amicon Ultra-15 centrifugal 3 kDa MWCO filter (Millipore, Cat# UFC900324), filter sterilized with using a Millex-GV syringe filter unit (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3) or GFP gene in the presence of polybrene (5 µg/ml; Sigma-Aldrich, Poland). Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich ...