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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... respectively using either glass micropipettes filled with 0.5 M NaCl solution containing with 1.5% w/v Biocytin (Sigma Aldrich, USA, impedance 3-25 MΩ) or Silicon probes (single shank ...
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bioRxiv - Neuroscience 2021Quote: 2-4 weeks following surgery anesthesia was induced with vaporized 3-4% isoflurane followed by IP injection of urethane (1.5 g/kg, IP; Sigma-Aldrich, St. Louis, MO, USA). Mice were placed back into their home cage for approximately 60 minutes until the toe-pinch withdrawal reflex was abolished ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney Vero’76 and human lung adenocarcinoma Calu-3 cells were maintained in DMEM (Sigma-Aldrich, D5796; St. Louis, MO, USA) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Microbiology 2021Quote: ... total genomic DNA was extracted from each strain by incubating approximately 3×109 cells in the presence of 20 mg/ml lysozyme (Sigma-Aldrich, St. Louis, MO) at 37 °C for 60 min ...
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bioRxiv - Biochemistry 2020Quote: ... Taurocholic acid sodium salt (97% pure) and estrone-3-sulfate sodium salt (containing 35% Tris stabilizer) were purchased from Sigma Aldrich (St. Louis, MO). Rosuvastatin (98% pure ...
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bioRxiv - Cell Biology 2021Quote: ... actinomycin D, indomethacin, streptavidin, EGF, (3-Glycidyloxypropyl)trimethoxysilane (GPS) (98%), Polydimethylsiloxane (PDMS, SYLGARD® 184) and DMSO were purchased from Sigma-Aldrich (Schnellendorf, Germany). BSA-Cy5 was obtained from Protein Mods (Madison ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, cat# M4655-500ML) containing 10% FCS and 1% PS ...
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bioRxiv - Cell Biology 2021Quote: ... The Cdh1fl/fl(Foxj1-CreERT2) mice were further given stepwise increases (0, 3 & 30 mg/mL) of Acetyl- β- methylcholine chloride (Sigma Aldrich, MO, USA), to assess airway responsiveness.
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bioRxiv - Molecular Biology 2021Quote: ... The constructs were screened via colony PCR and confirmed by sequencing (Sequencing Core Facility, SUNY, Albany, and Eaton Bioscience Inc. sequencing service) using the ACYCDuetUP1 (Novagen Cat. No.71178-3) and DuetDOWN1 primers (Novagen Cat ...
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bioRxiv - Microbiology 2021Quote: Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FCS and 1% PS.
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bioRxiv - Neuroscience 2020Quote: ... 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 2.5 glucose, 6.2 MgCl2, 0.1 CaCl2, and 3 kynurenic acid; Sigma-Aldrich GmbH; bubbled with 95% 02 / 5% CO2) after intracardial perfusion with ice-cold ASCF ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then filtered through a 100-kDa filter to remove any higher-ordered aggregates and then concentrated using a 3-kDa filter (Millipore Sigma, St. Louis, MO). Samples were diluted to a final protein concentration of 150-300 μM with 10% D2O added ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus strains were grown in lysogeny broth (LB) medium (Fischer Scientific, Pittsburgh, PA) containing 3% NaCl (LBS) at 37°C with aeration or M9 medium media (Sigma Aldrich, St. Louis, MO) supplemented with 3% NaCl (M9S) ...
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bioRxiv - Immunology 2021Quote: ... which were diluted 1:100 in triplicate into 5 mL fresh CHG media containing either 100 μM of the corresponding substrate (either LCA [Sigma] or 3-oxoLCA [Steraloids]). Cultures were grown for 48 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Microbiology 2023Quote: ... The CpG with a sequence of 5’-TCC.ATG.ACG.TTC.CTG.ACG.TT-3’ (all nucleotides are phosphorothioate-modified at the 3’ inter-nucleotide linkage) was purchased from Integrated DNA Technologies (IDT, Coralville, IA) and the IFA from Sigma-Aldrich (St. Louis, MO). We used the CpG-IFA as adjuvants for the intramuscular injection because they have been shown to induce Th1 dominant immune responses (68 ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Neuroscience 2022Quote: ... larval zebrafish were mounted in a custom 3D printed chamber (SLS Nylon 12, 3DPRINTUK, London, United Kingdom) in 3% low-melting point agarose (Sigma-Aldrich, St. Louis, Missouri) at 5 dpf and allowed to recover overnight before functional imaging at 6 dpf ...
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bioRxiv - Bioengineering 2024Quote: ... Phosphatase activity was determined by a chromogenic reaction using 5-bromo-4 chloro-3 indolyl phosphate and nitroblue tetrazolium (Sigma, St. Louis, MO, USA) as substrates.
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bioRxiv - Developmental Biology 2024Quote: ... cochleae were washed 3 times for 15 minutes in PBS and the sensory epithelium was dissected off and mounted (Sigma-Aldrich, Cat. No. F6182) on a glass slide ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3x with PBS prior to an incubation in permeabilization solution (0.1% Triton X-100 [Sigma-Aldrich, #T8787] and 3% BSA [Sigma-Aldrich, #10735078001] in PBS) for 5 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Microbiology 2023Quote: ... We serially diluted the extracts and plated 20 µL on 10 % TSB agar (3 g/L tryptic soy broth and 15 g/L agar, both Sigma-Aldrich, St. Louis, USA) plates (12 x 12 cm ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Evolutionary Biology 2023Quote: ... an animal was chemically cross-linked for one hour at room temperature in 30 mL of PBS 1X added with 3% formaldehyde (Sigma – F8775 - 4×25 mL). Formaldehyde was quenched for 20 min at RT by adding 10 ml of 2.5 M glycine ...
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bioRxiv - Bioengineering 2022Quote: ... They were allocated to 4 cohorts (20 flies of each) and transferred to the fresh food with RU-486 (Sigma, Cat #: 84371-65-3) added at a final concentration of 200 μM and raised in 25°C to allow the Gal4-UAS system function well ...
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bioRxiv - Genetics 2022Quote: Animals were raised and maintained from embryonic stages onward on normal food supplemented with 3OH-K (1mg/ml of 3-Hydroxy-DL-kynurenine, H1771, Sigma Aldrich; dissolved in DMSO) or KYNA (1mg/ml of Kynurenic acid ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Immunology 2022Quote: ... the animal hemi-heads were fixed for 3 days at room temperature in 4% paraformaldehyde (PFA) and decalcified in Osteosoft (Osteosoft; 101728; Merck Millipore; Saint-Quentin Fallavier; France) for 3 weeks ...
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bioRxiv - Microbiology 2023Quote: C57BL/6J wild type and LDLR KO (B6.129S7-Ldlrtm1her/J) mice were intraperitoneally injected by 3% brewer thioglycollate medium (Millipore-Sigma, St. Louis, MO, USA). Mouse peritoneal cavity elicited macrophages (MØ ...
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bioRxiv - Microbiology 2022Quote: ... parahaemolyticus strains were grown in Luria Bertani (LB) medium (Fischer Scientific, Pittsburgh, PA) containing 3% NaCl (LBS) at 37°C with aeration or M9 medium media (Sigma Aldrich, St. Louis, MO) containing 3% NaCl (M9S ...
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bioRxiv - Microbiology 2023Quote: Transductions were carried out at 3×105 cells/mL and the indicated MOIs under normal culture conditions supplemented with 5 µg/mL polybrene (Sigma-Aldrich, TR-1003-G). Culture medium was replaced ∼24 hours after transduction and antibiotic resistance selection was carried out using 1.2 µg/mL puromycin for 2 days ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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bioRxiv - Neuroscience 2023Quote: ... induction to an EB-like state was initiated by changing the medium to chemical induction medium (StemFit AK02N, (Ajinomoto) with 3 μM SB431542 (Sigma-Aldrich, St. Lous, MO), 3 μM CHIR9901 (Cayman ...
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bioRxiv - Microbiology 2023Quote: ... isolated areas on the slides were then placed in PBS for 3 minutes before permeabilizing with 0.5% Triton-X 100 (Sigma-Aldrich, St. Louis, MO, USA) for 20 mins ...
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bioRxiv - Bioengineering 2024Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DPPE-PEG5K) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was procured from Fisher Scientific (Pittsburg ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells then fixed for 5 min with 4% PFA followed by 3 times of PBS wash and then permeabilized with 0.1% of Triton X-100 (Sigma-Aldrich, Cat. no. X100-00ML) in PBS for 5 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The sample was centrifuged at 500 x g for 5 minutes at 4°C after which the supernatant was discarded and the nuclei resuspended in 3 ml ice cold nuclei suspension buffer (1x phosphate-buffered saline [PBS, Sigma-Aldrich, P4417-50TAB, US]), 0.01% bovine serum albumin [BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Auxin-inducible degradation of proteins was induced by treatment with 0.5 mM indole-3-acetic acid (IAA) (Sigma, I2886-5G, CAS: 87-51-4) and 4 µM phytic acid dipotassium salt (Sigma ...
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bioRxiv - Immunology 2024Quote: ... The reaction was visualized by subsequent addition of BCIP/5-bromo-4-chloro-3-indolylphosphate/nitro blue tetrazolium substrate (Sigma-Aldrich Cat#B5655-5TAB). The number of spots was determined using a CTL ImmunoSpot S5 UV Analyzer equipped with ImmunoSpot ImmunoCapture and ImmunoSpot Counting software (Cellular Technology Ltd. ...
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bioRxiv - Immunology 2024Quote: ... discs were stimulated for 3 hours with LPS [25 ng/mL] + 45 min with Nigericin sodium salt [20 μM] (Sigma® Cat. N7143-5MG). Supernatants were then collected and stored at -80°C until tested Bio-Techne ELISA kit for TNF-α (R&D Systems Cat ...
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bioRxiv - Immunology 2024Quote: ... IPI-549 was prepared at 15mg/kg or 30mg/kg of body weight in PBS with 40% (v/v) PEG-400 (Sigma-Aldrich #25322-68-3). Formulations were prepared fresh ...
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bioRxiv - Cell Biology 2024Quote: ... the cell culture medium was removed and replenished with a culture medium respectively containing carbonyl cyanide 3-chlorophenylhydrazone (CCCP; Sigma-Aldrich, MA, USA, #C2759), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid acetoxymethyl ester (BAPTA-AM ...
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bioRxiv - Plant Biology 2024Quote: ... apoplastic fluids collected from control and infected soybean roots at 3 days post infection (dpi) were preincubated with commercially available soybean Kunitz (Sigma Cat no. 93620-250MG) at concentrations 1.25 ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were either left unstimulated or activated for 3 days by adding phorbol 12-myristate 13-acetate (PMA) and ionomycin (Sigma-Aldrich Canada, Oakville, ON) (P/I ...