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Citations for Millipore Sigma :
7351 - 7400 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the supernatants were concentrated 50-fold using Amicon Ultra-4 centrifugal filters with a molecular weight cut-off of 3 kDa (Merck-Millipore, Burlington, MA, USA). Samples were mixed with 3× Laemmli loading buffer and heated for 3 min at 85 °C before SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2022Quote: ... dissolved in 50 μL water containing reference compounds (200 μM each of trimesate [Wako] and 3-aminopyrrolidine [Sigma-Aldrich, St. Louis, MO, USA]). Proteins were precipitated by adding 800 μL ice-cold methanol to the interphase and organic layers and centrifuged at 12,000 × g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 or PC-3 cells were transduced with virus-containing culture media supplemented with 10 µg/ml Polybrene (Millipore, Cat #TR-1003-G) for 24 hours and replaced with fresh growth media ...
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bioRxiv - Microbiology 2022Quote: ... at RT for 1 h to prevent non-specific staining and afterwards stained with 50 nM Halo-CF647 in PEMBAL buffer (1x PEM containing 3% BSA (Sigma-Aldrich, #A8549-10MG), 0.1% NaN3 (Carl Roth ...
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Timed deletion of the Gpx4 gene and fate-mapping was achieved using Sox9IRES-CreERT2 knock-in mouse line with 3 doses of intraperitoneal injections of tamoxifen (100 mg/kg body weight, Sigma, St. Louis MO) on alternate days ...
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bioRxiv - Biophysics 2020Quote: ... ammoniumpersulfate and 0.1 % (v/v) N,N,N,N-tetramethylethylenediamine onto freshly plasma-cleaned cover slips that were treated with 3-aminopropyltriethoxysilane (Sigma, Munich, Germany, A3648) and subsequently with a 0.05% glutaraldehyde solution (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained neurovascular units ...
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bioRxiv - Cell Biology 2021Quote: The oocytes were fixed overnight in 3% glutaraldehyde (AGR1012, Agar Scientific, Stansted, UK) in 0.1 M sodium cacodylate buffer (C0250, Sigma Aldrich, St. Louis, USA) (pH 7.2–7.8 ...
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bioRxiv - Bioengineering 2020Quote: ... The meniscus tissues were fixed with 4% paraformaldehyde for 3 hours at room temperature and then decalcified with decalcifying solution (Sigma, St Louise, USA). After decalcification ...
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bioRxiv - Biophysics 2020Quote: Solutions of 1 μM Aβ42 in the presence and absence of 6μM 10074-G5 were deposited on mica positively functionalized with (3-aminopropyl) triethoxysilane (APTES, Sigma Aldrich, St. Louis, USA) in the absence of ThT ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then rinsed with PBS (3×5 min at RT) and then cell nuclei were stained with a DAPI solution (Sigma-Aldrich, 1μg/mL) incubated for 5 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... purity ≥ 95%, fraction V fatty acid free) and hexadecanoic acid (CAS 57-10-3, natural, purity ≥ 98%) were from Millipore Sigma (Burlington, MA). HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) ...
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bioRxiv - Bioengineering 2020Quote: ... endothelial cells were enzymatically removed and subsequently epithelial cells were lysed with a protein lysis buffer (MSD, R60TX-3) containing protease (ThermoFischer, 78425) and phosphatase inhibitors (Sigma-Aldrich, P0044 & P5726). Protein extraction was performed according to standard protocols and the quantification of the total amount of protein/ sample was done by Bradford assay (Thermo Fischer ...
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bioRxiv - Immunology 2020Quote: ... 293T cells at 80% confluence were shifted to 320C and incubated for 3 days in the presence of the proteases inhibitors (Sigma, St. Louis, MO) to induced maximal protein production ...
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bioRxiv - Microbiology 2020Quote: The SPYMEG cells and established hybridomas were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and 3% BM-condimed (Sigma Aldrich, St. Louis, MO) [18] ...
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bioRxiv - Microbiology 2021Quote: ... were orally gavaged at the beginning of the light cycle (between Zeitgeber time 0-3) with 0.1 ml sesame oil vehicle (Sigma-Aldrich, St. Louis, MO) or 0.3 ...
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bioRxiv - Cell Biology 2021Quote: Dissected ovaries were treated for 2 h with inhibitors of aPKC (CRT-006-68-54, Bio-Techn Sales Corporation, 10 μM) or Pak1 (IPA-3, Millipore Sigma, 50 μM) as described (Chartier et al. ...
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bioRxiv - Genomics 2021Quote: ... Cells were then washed 3 times with PBS and incubated with 0.05 mg/ml Filipin III from Streptomyces filipinensis (Sigma; Cat. No. F4767-1MG) in PBS for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Transfected cells were supplemented three days after transfection with 3 g/L glucose (Bioconcept) and 5 g/L soy hydrolysate (Sigma-Aldrich Chemie GmbH). Cell culture supernatant (423 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... fresh sterile samples from resected tumors were minced in Dulbecco’s modified Eagle medium (DMEM; Corning, cat # 10013CV and incubated with collagenase type I (3%; Sigma-Aldrich, cat # C-0130), DNase I (0.02% ...
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bioRxiv - Systems Biology 2023Quote: ... the optical density of the preculture was measured at 600 nm (OD600) and the mycelia were harvested by centrifugation (3800 x g; 15 min; SIGMA 3-16K centrifuge) and washed twice with sterilized water ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of ddH2O was added to each well followed by 25ul of 50mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (Sigma, St Louis, Missouri) and 25ul of 50mg/ml N-Hydroxysuccinimide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 μM DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) and 4 μM TAT-cre (Millipore, cat. no. SCR508) overnight.
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bioRxiv - Cancer Biology 2023Quote: ... followed by transduction with LV at MOI 3 in the presence of 8 µg/ml Hexadimethrine bromide (Sigma-Aldrich, St. Louis, MO, USA) the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 10 M cells were used per each replicate of library and 3 µg of anti-CTCF antibody (Sigma-Aldrich, 07-729) was used for o/n chromatin immunoprecipitation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Bottles were spiked with a final concentration of 50 μM Diuron or 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; Sigma-Aldrich; St. Louis, Missouri, USA) to block oxygenic phototrophic activity54 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were well washed with 1x PBS to remove permeabilization solution before blocking with 3% w/v BSA (Sigma-Aldrich, Cat # A7906-100G) in 1x PBS overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: Embryos at stage 29-31E (before the pre-hatching period) were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) in natural seawater and sacrificed by decapitation ...
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bioRxiv - Physiology 2022Quote: ... The final concentration of SMX/TMP drinking water is 640µg/ml, 128 µg/ml (TMP) (CuRx™ SMX/TMP Antibiotic Cat# 20-0037, Yecuris corporation) and 3% Dextrose (Sigma-Aldrich ref D9434). The homozygous deletion of fah in FRGN mice which produces a fumaryloacetoacetate dehydrogenase deficiency that disrupts tyrosine metabolism ...
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bioRxiv - Cell Biology 2024Quote: ... cells were then resuspended with 3% BSA in PBS supplemented with 0.1% of NaN3 until acquisition on Amins ImageStreamx MKII (ISx, EMD Millipore, Seattle, WA, USA).
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bioRxiv - Cell Biology 2024Quote: The methods used are similar to those described in Shin et al.37 and Castellano et al.3 Cells were cholesterol-depleted using cell-culture media supplemented with methyl-β-cyclodextrin (MCD, Sigma-Aldrich, #C4555) and lipid-depleted serum (Lipoprotein Deficient Serum from fetal calf ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were eluted from Flag magnetic beads through adding 5 package of beads gel volume of 150 ng/μl 3 x Flag peptide (Sigma-Aldrich, cat # 4799) in 1 × TBS buffer and incubating in a rotator for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Washed pellets were resuspended in a 7:3 ratio of chemotaxis buffer (see above) and Optiprep (60% iodixanol in sterile ddH2O w/v, Sigma-Aldrich, D-1556), resulting in a net dilution to 18% iodixanol w/v ...
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bioRxiv - Pathology 2023Quote: ... The solvent (n=5) or an FXIa inhibitor (ONO-1600586, 3 µmol/L n=5) and mepacrine (Sigma-Aldrich; final concentration 5 µM) were added to the blood before perfusion ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained enriched neurovascular fractions ...
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bioRxiv - Plant Biology 2023Quote: ... GAA microsatellites on the isolated chromosomes were labelled by fluorescence in situ hybridization in suspension (FISHIS) using 5’-FITC-GAA7-FITC-3’ oligonucleotides (Sigma, Saint Louis, USA) according to (10 ...
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bioRxiv - Genetics 2024Quote: ... bulk embryos were isolated by vigorous mixing in hypochlorite solution (40 ml dH2O, 7 ml sodium hypochlorite Sigma #425044, 3 ml 1M KOH) for 6-7 minutes ...
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bioRxiv - Neuroscience 2024Quote: Compounds of interest were obtained from a wide range of reputable vendors (see Table 3) and resuspended in DMSO (Sigma-Aldrich, Cat #:472301). To keep the design of our experiment as similar as possible to the CMAP study ...
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bioRxiv - Genomics 2023Quote: ... 10 mL ATCC vitamin supplement and 1 mL vitamin K-3 solution (0.14 g vitamin K-3 in 100 mL 95% ethanol)24 reduced by the addition of 50% Oxyrase (SAE0013; Sigma, St. Louis, MO) were added in an anaerobic chamber ...
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bioRxiv - Biophysics 2023Quote: ... Lipid fluorophore 1,2 – Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647 (Atto 647 DOPE) was purchased from Sigma Aldrich (St. Louis, MO). Thapsigargin was used at 2 μM (AC328570010 ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885; 1.6 mg/ml Hyaluronidase, in HL-15, Sigma-Aldrich, #H4272), at 28°C and in HL-15 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The colorimetric signal was subsequently detected using nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma-Aldrich, MO, USA) at 37 °C for a duration of 10 minutes.
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bioRxiv - Immunology 2024Quote: 48h CD8+ T-cells were collected at 48h post-activation and 3×106 cells were washed twice with ice cold 0.9% saline made with NaCl (Sigma Aldrich, 31434-500G-M) and ultrapure HPLC grade water (Alfa Aesar ...
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bioRxiv - Biochemistry 2024Quote: ... and the samples were allowed to cool to room temperature before adding 400 μl 12 mg/ml ammonium molybdate tetrahydrate (EMD Millipore AX1310-3) and 400 μl 50 mg/ml ascorbic acid (Sigma A5960-100G) ...
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bioRxiv - Immunology 2022Quote: Collection of peritoneal lavage fluid and peritoneal exudate cells (PECs) was performed by flushing the peritoneal cavity with 3 ml ice cold RPMI 1640 media (Sigma-Aldrich, Dorset, UK) containing 1 % Penicillin/Streptomycin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were harvested at E11.5 and N=3 WT and mutant forelimbs (pooled left and right) were dissociated into single cell suspensions using collagenase (Sigma C9697; 50mg/mL) at 37°C for 15 minutes followed by gentle pipette mixing ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...