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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then extensively washed in lysis buffer and elution was carried out with 50 μL of 3×FLAG peptide (Sigma-Aldrich #F4799), the supernatant was separated from the beads by centrifugation ...
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bioRxiv - Pathology 2021Quote: ... (groups 2 & 3) treated groups including mice receiving daily intraperitoneal injection of 150 mg/kg/day of β-aminopropionitrile fumarate (BAPN, A3134; Sigma Aldrich) for 28 days ...
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bioRxiv - Developmental Biology 2020Quote: ... H2B was amplified using Q5 Hot Start High-Fidelity DNA Polymerase and then cloned into pPIDNB with QuikChange (Liu and Naismith, 2008) using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-3). To generate pPIDNB2-DHB ...
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bioRxiv - Genomics 2021Quote: ... cells were first washed once with phosphate-buffered saline (PBS) and supplemented with equilibrated (37 °C and 5% CO2) medium containing 500 μM indole-3-acetic acid (Sigma-Aldrich, I5148) freshly prepared before use ...
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bioRxiv - Immunology 2020Quote: Tyrosine hydroxylase activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalogue number: 18250), 30 min before activation with anti-IgM/CpG.
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bioRxiv - Genetics 2020Quote: ... The plates were centrifuged at 1500 rpm for 3 min to remove any debris and 10 μl of the supernatant and triglyceride standards (Sigma: G7793-5ML) was added to 20 μl of triglyceride reagent (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Methyl methanesulphonate (MMS) (#129925) (CAS registry number 66-27-3) and carbendazim (#378674) (CAS no. 10605-21-7) were purchased from Sigma-Aldrich (Merck), UK.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mice were anesthetized and injected intravenously with 200 μL of Evans blue solution containing 3% Evans blue fluorescent dye (Sigma-Aldrich; #E2129) in 5% BSA/PBS to visualize vasculature prior to organ extraction and ex-vivo multiphoton imaging.
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and oligo(dT) primers (Eurofins Genomics) for detection of mRNA or a fluA uni12 forward primer [92] (Sigma-Aldrich, 5’-AGCAAAAGCAGG-3’) to detect vRNA according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mg of cell extract was incubated with 60 μl/sample of Anti-FLAG® M2 Magnetic Beads (Millipore Sigma, M8823-1ML) overnight on a rotating wheel at 4C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and samples were incubated in blocking solution (3% powdered nonfat milk in PBS + 0.1% IGEPAL CA-630 (Sigma-Aldrich, 18896, lot 1043) for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... fresh spikelet and inflorescence samples were fixed in 0.1 M sodium phosphate buffer pH 7.0 containing 3% glutraldehyde (Sigma-Aldrich, St. Louis, USA) for 12-24 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 4-day old Col_0 seedlings were incubated with LRC liquid medium supplemented with indicated concentrations of compounds (Adenosine 5′-triphosphate magnesium salt, ATP-Mg [VWR]; Abscisic acid, ABA [Sigma-Aldrich]; Carbonyl cyanide 3-chlorophenylhydrazone, CCCP [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were washed for 3 × 5 minutes washes in Duolink Wash Buffer A and then incubated in 40 µL of ligase (Sigma Aldrich, DUO82027) at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were washed for 3 × 5-minute washes in Duolink Wash Buffer A and then incubated in 40 µL of polymerase (Sigma Aldrich, DUO82028) at 37 °C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma, X100] in 1xPBS) and then with primary antibodies for 2-3 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... we concentrated ∼75 mL of virus to ∼3 mL (25x concentration) using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma; UFC905008). All viral and lentiviral infections and transductions were done in the presence of 20 μg/mL DEAE-Dextran (Sigma ...
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bioRxiv - Bioengineering 2022Quote: The HS-5 cell line (ATCC) and JJN-3 cell lines were both STR profiled to ensure accurate identification and maintained in RPMI (Sigma-Aldrich, R8758) with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Then the coverslips are placed in a closed chamber with 150 µl of (3-aminopropyl) triethoxysilane (Sigma-Aldrich, Saint-Quentin Fallavier, France) and a desiccator (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
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bioRxiv - Genetics 2024Quote: ... except for (ii) were assembled together (in the order described in Figure S2) using restriction free methods and cloned into pET28a (Novagen #69864-3) plasmid by the cloning unit in Weizmann institute ...
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bioRxiv - Cell Biology 2024Quote: PA-JEB/β4 cells stably expressing Caskin2-BirA* fusion proteins were grown to 70%-80% confluence on 3 × 145 mm plates in biotin-depleted keratinocyte growth medium before they were treated with 50 μM biotin (Sigma-Aldrich #B4501) for 20 h at 37°C in DMEM/10% FCS ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Immunology 2023Quote: ... Membranes were again washed with TBS-T for 3 times and were incubated with Immobilon Forte Western HRP substrate (WBLUF0500, EMD Millipore Corporation). Images were captured using AI600 Imager from GE Healthcare Life Science.
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bioRxiv - Plant Biology 2023Quote: ... Separate 50 µl (same aliquot from the total 300 µl) were separated on 13% SDS-PAGE gel for immunoblot using anti-Histone 3 (Sigma #06-755) at 1:3000 dilution with 1:10000 anti- rabbit secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... virus stocks of the WN-KOUTV isolate and the previously isolated Kenyan WN Lineage 1a strain (AMH005348) were generated from passage 3 in Vero E6 (Ceropithecus aethiops) cells (Sigma Aldrich, France) and used in this assay ...
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bioRxiv - Cell Biology 2024Quote: ... and ABTS substrate solution (water containing 0.54mg/mL w/v 2’2-Azinobis (3-ethylbenzthiazoline Sulfonic Acid) diammonium salt (Sigma-Aldrich, Cat#A-1888), and 0.1M Citric Acid and 0.03% v/v Hydrogen Peroxide (H2O2) ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in DPBS for 30 minutes at room temperature and blocked by incubation in bovine serum albumin (BSA, 3% weight/vol, Sigma Aldrich #A2153) diluted in DPBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... cumulous-enclosed egg complexes were collected from the oviduct and cultured in 37°C MEM with hyaluronidase (3 mg/ml, Sigma Aldrich, H4272) for 2 min ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed with the isopropanol (IPA) for 3 hours then rehydrated in DI and treated with 50U/ml DNase (Millipore Sigma, #10104159001) for 8 hours followed by an overnight DI rinse ...
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bioRxiv - Cell Biology 2023Quote: ... Digested tubules were strained on a 100 μm strainer containing 3 ml of FBS (100% Fetal Bovine Serum, heat inactivated, Sigma F4135-500mL). Total germ cells were centrifuged at 300 xg for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended at 10 million cells per ml and DAPI added immediately before FACS (Sigma-Aldrich; final concentration of 3 μM). Live ...
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bioRxiv - Cell Biology 2023Quote: ... H3.3 K36M or cells overexpressing histones were continuously grown in the presence of 50μM Indole-3-acetic acid (IAA) (Sigma-Aldrich, I5148-2G) until experiments were performed to suppress protein expression ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of freshly dissolved histones was added to a premixed solution containing 3 μL of 10 mM Cy5-azide (Sigma-Aldrich, 777323), 10 μL of a 3:7 mixture of 50 mM CuSO4 and 100 mM THPTA ...
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bioRxiv - Physiology 2023Quote: ... MEFs were selected with 3 μg/mL of puromycin and transduction efficiency for mCherry was assessed by flow cytometry using Guava EasyCyte™ (Millipore, USA). A transduction efficiency of 95% or greater was achieved.
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Immunology 2023Quote: ... 5 mg of DOPC and NTA lipids (Avanti Biosciences, USA) were transferred to tubes with 3 mL of chloroform (Sigma Aldrich, USA) dried under a stream of nitrogen gas ...
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bioRxiv - Developmental Biology 2024Quote: DNA and RNA were extracted from pools of 10 3 day-old embryos or ventricles of adult zebrafish hearts using TRI Reagent® (Sigma, T9424) followed by phenol-chloroform extraction ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were diluted in [3 % Bovine Serum Albumin (BSA) fraction V (Fisher, BP1600-100) in PBST and 0.1 % Sodium Azide NaN3 (Sigma Ultra, s-8032)] as follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Bioengineering 2022Quote: ... cells were grown to 100% confluency and then fed 3 times a week with adipogenic medium (growth medium with 10 μg/mL insulin (Sigma Aldrich #I0516), 0.5 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Biochemistry 2022Quote: ... using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) substrate at 405 nm (Sigma Aldrich Catalog # 11468120910).