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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... fixed in 40% isopropanol by three-minute incubation at room temperature and incubated with 3 mg/ml Oil Red O (Sigma, 09755) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Sub culturing was done once in 3-4 days and involved pelleting and washing cells with PBS followed by incubation with accutase (Sigma-Aldrich) and repeated pipetting in NSM medium to dissociate the spheroids ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed in 250 μl of buffer P1 by vortexing for 3 minutes in the presence of glass beads (G8772, Sigma-Aldrich). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 followed by blocking for 1hr in PBS containing 0.2% (v/v) Triton X-100 and 3% (w/v) bovine serum albumin (A9418 Sigma-Aldrich, UK). Primary antibodies for ALDH1A1 (PA5-32127 ...
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bioRxiv - Systems Biology 2023Quote: ... the media was replaced by first washing twice with 3 mL DPBS+/+ (Phosphate buffered saline containing Mg++ and Ca++, Sigma, D8662) and then adding NDiff227 media (N2B27 ...
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bioRxiv - Genetics 2023Quote: BCL11A enhancer DHS +58 and +55 core regions were amplified with KOD Hot Start DNA Polymerase (EMD-Millipore, cat# 71086-3) and corresponding +58 Sanger primers ...
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bioRxiv - Microbiology 2023Quote: ... 20 mM glucose final concentration; 10 g tryptone [Fisher Bioreagents, BP1421], 5 g yeast extract [Fisher Bioreagents, BP1422], 3 g K2HPO4 [Sigma-Aldrich, P3786] ...
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bioRxiv - Immunology 2023Quote: ... mice were orally gavaged with 150 μL of an 80 mg/mL solution of 3-5 kDa-FITC-Dextran (Sigma-Aldrich) in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Biophysics 2023Quote: ... the substrates were kept in a hermetically sealed case with a 50-ml aliquot of liquid silane (3-aminopropyl triethoxysilane, Sigma-Aldrich) at 70 °C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The different polymers were incubated in the presence of 10 eq N-ethyl-N’-(3- (dimethylaminopropyl)carbodiimide (EDC) (Tokyo Kasei) and 15 eq N-hydroxysuccinimide (NHS) (Sigma-Aldrich) in water at pH 4.5 for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... the imidazole was removed by running the eluted sample on a 3 mL Sephadex G-50 size exclusion column (Sigma-Aldrich). The labeling yield was quantified by placing the sample in a 1 cm quartz cuvette in a Nanodrop 2000c UV-VIS spectrophotometer and measuring the absorbance spectrum between 190-840 nm ...
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bioRxiv - Bioengineering 2022Quote: ... they are placed face down on a holding fixture within a glass jar with 50 uL of 3-Glycidoxypropyl trimethoxysilane (GPTMS) (Sigma Aldrich) at the bottom ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested using Accutase (#LM-T1735, Biosera) and diluted in PBS with 3% fetal bovine serum (#FB-1101, Biosera) and 2mM EDTA (#ED2SS, Sigma-Aldrich) and immediately processed for measurements using CytoFLEX S flow cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Triton-X 100 in PBS with 5 min incubation between each wash and blocked using 3% bovine serum albumin (BSA, A2058, Sigma-Aldrich) in PBS for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... frozen cell pellets were resuspended in 200 μl of 20 % TCA on ice and lysed with vortexing for 3 minutes with acid-wash glass beads (Sigma-Aldrich). The glass beads were washed with 200 μl of 5% TCA twice and the crude extract was collected to a new tube ...
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bioRxiv - Cell Biology 2023Quote: ... the column was washed with 20 column volumes of the lysis buffer and the protein was eluted with 3 mM desthiobiotin (Sigma, D1411).
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bioRxiv - Biophysics 2023Quote: ... and the hACE2 stably expressing cell lines were selected and maintained with medium containing 3 μg/ml puromycin (Sigma, Missouri, USA). hACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting DNA was pipetted along one side of a coverslip that had been placed on top of a 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slide and allowed to enter by capillary action ...
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bioRxiv - Microbiology 2023Quote: ... plated on fluorodishes were incubated in 50mM NH4Cl for 5min at room temperature (RT) to quench free aldehydes and in 1X PBS containing 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) for 15min at RT to block non-specific epitopes ...
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bioRxiv - Biochemistry 2022Quote: Protease treatment of >30kDa FBS fraction was performed by treating 200 µL of large fraction (previously washed 3 times with 1X PBS) with 10 µL of 20 mg/mL Proteinase-K (#SAE0151, Sigma-Aldrich) at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: Acellular stretched and nonstretched hydrogels in 3 mg/mL and 6 mg/mL concentrations were created and fixed in 3.7% formaldehyde (Sigma-Aldrich 252549) in PBS at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: Precultures were grown in mineral media (Figure 3) or buffered SD media supplemented with 2000 µg/L thiamine (thiamine HCL, Sigma-Aldrich) (Figure 5 ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was changed to the same base medium with the addition of CHIR99021 (Chiron, 3 µM, Sigma-Aldrich SML1046-5MG), PD0325901 (1 μM ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (w/v) BSA and 3% (v/v) serum of the same species as secondary antibodies were raised in (ordered from Sigma-Aldrich). Following blocking ...
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bioRxiv - Cancer Biology 2023Quote: Decellularized matrices were fixed for 30 minutes under gentle agitation (12 rpm orbital 154 shaker) in 3 % glutaraldehyde solution in cacodylate buffer (100 mM, Sigma 20840) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 1x PBS and incubated until analysis in cell culture medium supplemented with gentamicin (Sigma, G1272). As positive control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... autologous plasma samples were taken from the top layer and the “buffy coat” was extracted and washed 3 times with 1x PBS (Sigma-Aldrich) at 300 x g ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... except that 3 µl beta-mercaptoethanol per sample replaced 1,4-dithiothreitol (DTT) and 0.5% polyvinylpyrrolidone (PVP, mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer ...
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bioRxiv - Physiology 2023Quote: ... Gene ablation was induced starting at 3 months of age using intra-peritoneal (20 mg/kg per day for 5 days; Sigma #T5648) and then chow-mediated intake (40 mg/kg ...
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bioRxiv - Microbiology 2023Quote: ... 50µL of NaCl were then added to the strip before being centrifuged (19,000 x g for 20 minutes, Sigma 3-16PK) to extract the tears ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 μl of the supernatant were transferred to a new tube and mixed with 3 volumes (900 μl) of Trizol LS (T3934, Sigma-Aldrich) by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed at 8 hr post-transduction and cells were maintained in fresh growth medium for 48 hr before undergoing 3 µg/mL puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Neuroscience 2023Quote: ... a sense sequence based on 5’-GCCCTAATCCAGAATCTTGTA-3’ corresponding to nucleotides 2473-2493 of mouse mDia3 (DIAPH2; NM_172493.2; Sigma-Aldrich, Cat# TRCN0000108782) was used to construct f(U6 ...
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bioRxiv - Neuroscience 2023Quote: ... we added 50 μL of primary antibody mix (see Table 3) diluted in FACS buffer containing HBSS with 0.4% bovine serum albumin (Sigma-Aldrich, #A3983), 15mM HEPES (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Microbiology 2023Quote: ... Protein knock-downs using the minimal Auxin-Inducible Degron (mAID) system were performed under 500 µM auxin (IAA: 3-indoleacetic acid in 100% ethanol; Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Biopsies were subsequently microtome sectioned to a thickness of 3 µm and mounted onto poly-L-lysine coated Poly-Prep glass slides (Cat. No. P0425-72EA, Sigma Aldrich). After incubating the slides at 37 °C over night deparaffination was performed ...
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bioRxiv - Cell Biology 2023Quote: ... 1.7 μM Acquity UPLC BEH C18 Column with a gradient of solvent A (97:3 H2O:methanol, 10 mM TBA (Sigma Aldrich #90781), 9 mM acetate (Fisher #11350) ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Evolutionary Biology 2023Quote: Total RNA was extracted from ∼150 injected animals (3 days old planulae) with the aid of Tri- Reagent® (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were imaged under a microscope after the addition of an anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2xSSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Neuroscience 2024Quote: ... These double transgenic animals were separated into 4 groups (n = 3) and injected with a single dose of 4OH-Tx (Sigma-Aldrich). Tissue was collected at 10- ...