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Citations for Millipore Sigma :
6901 - 6950 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The mAbs were buffer-exchanged against incomplete culture medium (25 mM Hepes, 0.37 mM hypoxanthine, and 2 mM L-glutamine in RPMI (Sigma, R0883)) ...
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bioRxiv - Microbiology 2020Quote: Active and pseudotyped SARS-CoV-2 particles were immobilized by adding 0.01% Poly-L-Lysine (PLL, Sigma Aldrich, MIS, USA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
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bioRxiv - Neuroscience 2020Quote: ... The surface of the coverslip facing the cortical surface was coated with poly-L-lysine (P4707; Sigma-Aldrich, Taufkirchen, Germany) and a sparse layer of 40 nm fluorescent beads (yellow-green FluoSpheres™ ...
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bioRxiv - Microbiology 2021Quote: ... (25 mg l−1) and sodium salicylate (from 0.1 mM to 2 mM) All reagents were purchased from Sigma Aldrich, except for X-Gal ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% heat-inactivated AB human serum, antibiotics (penicillin, 200 U/ml; and streptomycin, 0.1 mg/mL) and L-glutamine (1 mM) (Sigma-Aldrich) at a concentration of 1 x 107 cells/mL ...
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bioRxiv - Microbiology 2021Quote: ... and Certified Multielement Ion Chromatography Anion Standard Solution for Bromine and Sulfur quantification (10 mg l-1, TraceCERT, Sigma-Aldrich) were also used to calibrate these respectively elements ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture plates (Denville Scientific, Inc., Plainfield, NJ) were coated overnight with poly-L-lysine (50 µg/mL; Sigma-Aldrich), supplemented with 7.5 µg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Aminopeptidase activity was assessed by fluorescence assays using the fluorogenic peptide L-leucine-7-amido-4 methylcoumarin hydrochloride (Sigma L2145) as a substrate as previously described (19) ...
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bioRxiv - Neuroscience 2020Quote: ... The following fatty acid supplementations were made to the standard culture media (all fatty acid-supplemented cultures also contained L-carnitine supplemented at 30uM and fatty acid-free bovine serum albumin at 1uM; all from Sigma): Docosahexaenoic acid (10 µM) ...
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bioRxiv - Microbiology 2021Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma-Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
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bioRxiv - Microbiology 2021Quote: ... Both pre-cultures and main cultures were supplemented with 0.025% (w/v) natural L-lysine (“light” lysine, Lys0, K0, Sigma Aldrich). The expression of hipA was induced with 0.2% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Terminal cellular differentiation was induced with 10 μM N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT; Sigma-Aldrich) while mucus-secretion was enhanced with 10 nM phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Molecular Biology 2020Quote: ... Spheroplasts were carefully washed twice and resuspended in 1 mL of cold spheroplast buffer and 200 µL were attached to poly-L-lysine (Sigma)-coated coverslips for 30 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and COS-7 (African green monkey kidney fibroblast cell line) were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/F12) with L-glutamine (Sigma, D8062) supplemented with 10% FBS (Sigma-Aldich ...
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bioRxiv - Microbiology 2020Quote: ... 2 g/L drop-out mix synthetic minus uracil without yeast nitrogen base (US Biological)) containing 2% (wt/vol) glucose (Sigma) and incubated at 30 °C for 24 h with shaking (250 rpm) ...
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bioRxiv - Microbiology 2019Quote: ... the slides were incubated for 2 hours at room temperature with 1 µg/mL Cy3-conjugated goat anti-human IgG (H+L) (Sigma) and washed again ...
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bioRxiv - Microbiology 2021Quote: ... The medium contained (amounts given are for 1 L): oat spelt xylan (0.6 g; Sigma-Aldrich, St. Louis, MO, USA), pectin (citrus ...
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bioRxiv - Microbiology 2020Quote: ... transferred to DMEM (1000 mg/l) and media glucose concentration was measured over a 24 h period using a glucose assay kit (Sigma). Glucose levels were expressed relative to media collected from worms which received naïve IgG (negative control) ...
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bioRxiv - Neuroscience 2021Quote: ... The recording surfaces of the MEAs were then coated by adding 0.5 ml of poly-l-lysine (PLL) (Sigma Aldrich) solution (0.1 mg/ml in ultra-pure water ...
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bioRxiv - Cell Biology 2021Quote: ... 24 or 48 hours after transfection the media was substituted with DMEM with 10%FBS and 5mM Nε-Acetyl-L-lysine (A4021, Sigma). Cells were lysed or fixed after being 24 hours in the presence of acetylated lysine ...
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bioRxiv - Cancer Biology 2020Quote: 50,000 cells (cell lines) or 250,000 cells (spike-in and patients samples) were applied onto poly-L-lysine hydrobromide (PLL) (Sigma Aldrich) -coated microscope cover glasses (24×60 mm ...
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bioRxiv - Cell Biology 2021Quote: ... after which cells were exchanged into Air medium (DMEM without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate (Sigma). The following were added ...
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bioRxiv - Cell Biology 2021Quote: 5 mL of yeast cells expressing the indicated constructs (see main text) were grown at 30°C in liquid YNB media (6.8 g/L) (Sigma-Aldrich) supplemented with 5 g/L glucose and 1.92 g/L synthetic drop-out medium without uracil (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... 2 mM L-glutamine and antibiotics (1,000 U/ml penicillin and 0.1 mg/ml streptomycin) (all from Sigma-Aldrich, UK). Cellular membranes where labelled with di-4-ANEPPDHQ (Di4A ...
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bioRxiv - Biophysics 2020Quote: ... Sections were mounted onto number 1.5 glass coverslips (Menzel-Glaser; Germany) coated with 0.05% (w/v) poly-L-lysine (Sigma-Aldrich, MO).
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bioRxiv - Biophysics 2021Quote: ... Seeds were sown on the surface of petri plates containing 0.8% phytoagar supplemented with one-half-strength Murashige and Skoog (MS) medium containing 2.15 g L−1 MS nutrient mix (Sigma-Aldrich), 1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were plated at a density of ∼3 × 105 cells on 12-mm glass coverslips coated with 0.01% poly-L-lysine (Sigma, P4832). Half of the medium was changed every 3-4 days and experiments were performed at 12–13 days in vitro.
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bioRxiv - Cell Biology 2021Quote: SK-N-SH-N cells were plated onto 6-well tissue culture plates coated with poly-L-lysine (Sigma-Aldrich). Cells were plated at 3×105 cells/ml in MEMα medium containing a very low-serum level (2% FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice with PBS, and changed to methionine/cystine/glutamine-free DMEM (Thermo, 21013024) supplemented with 0.5 mM L-cystine (Sigma, 57579) and L-glutamine (4 mM ...
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bioRxiv - Cancer Biology 2022Quote: Five micrometers thick paraffin sections of 4% PFA fixed tissues were cut and mounted on poly-L-lysine (Sigma Aldrich) coated slides ...
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bioRxiv - Neuroscience 2022Quote: ... demyelinating lesions were induced in the spinal cord by a stereotaxic injection of 0.5µ L of 1% lysophosphatidylcholine (LPC, Sigma Aldrich) in sterile 0.9% NaCl solution ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with fluorescently labeled gelatin as previously described (31) or with 50 μg/ml poly-L-lysine (Sigma-Aldrich) for 20 min and let to air dry ...
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bioRxiv - Biophysics 2022Quote: A 10 μL droplet of 0.01 μg/ml of poly-L-ornithine (1 kDa Mw, Sigma-Aldrich, St. Louis, MO) was deposited onto freshly cleaved mica and incubated for 2 min ...
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bioRxiv - Immunology 2022Quote: ... Poly-l-lysine (Cat no.-P-8920) and Albumin from chicken egg (Cat no.-A5503-1G) were purchased from Sigma. Tween-20 detergent (Cat no.-655205-250ML ...
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bioRxiv - Physiology 2022Quote: ... following the manufacturer’s protocol with siRNA targeting STK25 (On-TargetPlus siRNA, Horizon Discovery, L-004873-00-0050) or siRNA targeting PRKAR1A (EHU071341, Sigma) for protein knockdown or non-targeting control siRNA (ON-TARGETplus non-targeting pool ...
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bioRxiv - Cancer Biology 2022Quote: Oleoyl-L-α-lysophosphatidic acid (LPA, L7260) and a Periodic Acid-Schiff (PAS) Kit (395B) were purchased from Sigma-Aldrich. The PKD inhibitor CRT0066101 (A8679 ...
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bioRxiv - Plant Biology 2022Quote: ... coli lysate and 400 μl of plant lysate was mixed and incubated with 10 μl of equilibrated Glutathione High-Capacity Magnetic Agarose Beads (SIGMA) for 1 h at 4 °C on a spinning wheel ...
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bioRxiv - Molecular Biology 2022Quote: ... Saliva was collected for 30 min and stocked in tubes containing 130 μL of cell culture medium (L-15 Medium, Sigma). One limitation of this technique ...
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bioRxiv - Immunology 2022Quote: ... ileal tissue was collected and stored in stabilization buffer (2 mM ethylenediaminetetraacetic acid [EDTA; Invitrogen AM9260G], 2mM L-glutamine [Gibco 25-030], and 0.5% bovine serum albumin [BSA; Sigma-Aldrich A9418] in Hank’s balanced salt solution [HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated hippocampal neurons were seeded onto 18-mm or 25-mm coverslips (Carolina Biologicals) coated with poly-L-lysine (1 mg/ml, Sigma) at a density of 25-40 × 103 cells/cm2 in Neurobasal media (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 1000-2500 PGCs were sorted into 500µL of conditioned L-15 medium (10% FBS, 50 U/mL penicillin + 50 μg/mL streptomycin (Sigma P4458), adjusted to mOsm 340±5 with 60% sucrose ...
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bioRxiv - Microbiology 2022Quote: ... cells (ATTC No. CCL-34) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 2mM L-glutamine (Sigma-Aldrich), antibiotics (1x PSN ...
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bioRxiv - Molecular Biology 2022Quote: ... N-acetyl-L-cysteine (NAC; #S0077) and methylthiazolyldiphenyl tetrazolium bromide (MTT; #2128) were products from Sigma-Aldrich (St Louis, MO). Mitochondria-targeted antioxidant (MitoQ ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 5 to 6-month-old mice were placed on a platform in a 1.7 L rubber-sealed glass chamber (Ikea, Älmhult, Sweden) containing a small amount of the carbon dioxide scavenger soda-lime (Sigma). The flurothyl solution was infused with a syringe pump at a 6 mL/hour rate until the first generalized tonic-clonic seizure (GTC ...
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bioRxiv - Neuroscience 2022Quote: ... were transferred individually into vials containing standard cornmeal diet or nutrient of interest (sucrose: Sigma #S7903, L-glucose: Sigma #G5500, lactic acid: Sigma #69785, yeast extract: Sigma #Y1625 ...
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bioRxiv - Neuroscience 2022Quote: ... were transferred individually into vials containing standard cornmeal diet or nutrient of interest (sucrose: Sigma #S7903, L-glucose: Sigma #G5500, lactic acid: Sigma #69785 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells used for experiments were plated on glass coverslips (#10337423, Fisher) or standard plastic cell culture dishes coated with poly-L-ornithine (#P3655, Sigma) and laminin (#L2020-1MG ...
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bioRxiv - Plant Biology 2021Quote: ... For in vitro growth seeds were surface sterilized and sown on solidified basal ½MS medium (2.2 g/L; M5519, Sigma Aldrich) containing 1.0% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... the hippocampal cells were seeded on a coverslip (12 mm diameter) coated with poly-L-lysine in DMEM (Sigma-Aldrich) and incubated for 4 h at 37°C ...