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Citations for Millipore Sigma :
6851 - 6900 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... were seeded on coverslips and incubated at their indicated oxygen concentrations for 24 h prior to treatment with 10 μmol/l bromodeoxyuridine (BrdU) cell proliferation labeling reagent (Sigma) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... and 1,2-diacyl-sn-glycero-3-phospho-L-serine mixture (PS mixture from bovine brain) was bought from Sigma-Aldrich, Dorset ...
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bioRxiv - Synthetic Biology 2020Quote: ... 90,000 HEK293::TLR cells were seeded into each well of a 48 well plate that was pre-coated with poly-l-lysine (Sigma). Immediately after seeding ...
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bioRxiv - Immunology 2019Quote: ... Nunc Maxisorb 96-well plates were coated with 100µl poly-l-lysine H20 at 20 µg/ml (Sigma, cat # P4832) overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Neuroscience 2019Quote: ... and was gently separated and stuck to a cover glass that has been coated with poly-L-lysine (Sigma-Aldrich) in a bubble of solution ...
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bioRxiv - Genomics 2019Quote: Transfected islet cells cultured on poly-L-lysine coated glass coverslips were fixed with ice-cold methanol and permeabilized with 0.5% (wt/vol) saponin (Sigma-Aldrich). The coverslips were first incubated with antibodies against Ki67 (dilution 1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... and eluted with 4 x 1 ml elution buffer (50 mM Tris-HCl pH 8.5, 500 mM NaCl, 20 mM reduced L-glutathione (Sigma G4251) and 0.1% TX-100 ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuging to remove the medium and suspending the pelleted cells at 5 × 106 cells/mL in a solution containing 20 g/L bovine serum albumin (Sigma), 0.2% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were thawed on 0.2% gelatin in serum/LIF and after 24h seeded at a density of 0.6 x105 cells/cm2 in 2i/LIF medium on a dish coated with 0.01% poly-L-ornithine (Sigma-Aldrich, P3655) and 500 ng/ml laminin (Corning ...
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bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μL of the mixture was added per well to a 96-well Multi-Screen HTS GV sterile filtration plate (Millipore), buffer was removed by vacuum ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in MDCK medium and after inoculation in infection medium containing N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (3µg/mL; Sigma, T1426).
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bioRxiv - Bioengineering 2021Quote: ... in 100 mM sodium phosphate - 5 mM ethylenediaminetetraacetic acid (EDTA) buffer (pH 6.5) with 10 mM L-cysteine–hydrochloride (all from Sigma-Aldrich) at 60 °C and 10 rpm for 18 hours ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
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bioRxiv - Bioengineering 2020Quote: ... followed by alternated layers of hyaluronic acid (HA at 1 mg/mL, Lifecore, USA) and poly(L-lysine) (PLL at 0.5 mg/mL, Sigma, France). The film crosslinking level was controlled by incubating the coated scaffolds in 30 or 70 mg/mL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were cultured in 5 ml osteogenic media (DMEM, 10% FBS, 1% P/S/F, 50 μg/ml L-ascorbic acid (SIGMA), 100 nM dexamethasone (SIGMA) ...
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bioRxiv - Bioengineering 2021Quote: ... the precursor solution for the Zn0.4Fe2.6O4 nanoparticles was prepared at a con-centration of 0.2 molZn+Fe / L in a 3:1 volume ratio of xylene and acetonitrile (both Sigma-Aldrich). Iron acetylacetonate (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Coverslips with the attached cells were placed in a 60 x 15 mm tissue culture dish containing complete media supplemented with 50 μg/mL L-sodium ascorbate (Sigma) and incubated for 10 days ...
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bioRxiv - Bioengineering 2020Quote: ... Lyophilized samples were then digested in papain solution (100mM Na2PO4, 5mM N-Acetyl-L-Cysteine, 5mM EDTA, 3.875 U/mL Papain (Sigma-Aldrich)) at 65°C overnight ...
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bioRxiv - Bioengineering 2020Quote: Tissues were incubated in an overnight papain digest (1.6 U/mL papain, 10 mM L-cysteine; both from Sigma-Aldrich) in a 60°C water bath ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was initiated by the addition of 1 mM N-α-benzoyl-L-arginine-p-nitroanilide substrate (Sigma-Aldrich) and incubated for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... was destarched by incubation with amylase (0.2 mL, Termamyl 120 L, alpha-amylase from Bacillus licheniformis – Type XII-A, Sigma-Aldrich) in sodium phosphate buffer (125 mL ...
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bioRxiv - Biophysics 2021Quote: ... dishes were coated with a 0.01 % (w/v) poly-L-lysine solution (MW 150,000 – 300,000 Da, Sigma-Aldrich, Munich, Germany) before cell seeding.
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... the dried metabolite pellets were resuspended in 90 μl of the LC-MS-grade water (Milli-Q 7000 equipped with an LC-Pak and a Millipak filter, Millipore). Then ...
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bioRxiv - Neuroscience 2021Quote: ... and aphidicolin (7.5µM) before cells were treated for 3 hours with nutrients (1 µM insulin plus 0.8 mM L-leucine [Sigma Aldrich catalog numbers I5500 and L8000 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were mechanically dissociated with a Pasteur pipette and plated at 100 cells/mm2 on poly-L-lysine (0.25 mg/ml, Sigma-Aldrich) coated coverslips in DMEM (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at a density of 100.000cells/cm2 in 12w plates pre-coated with poly-L-lysine (Sigma-Aldrich). Cultures were maintained at 37°C and 5% CO2 in a humidified atmosphere ...
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bioRxiv - Neuroscience 2021Quote: For neuron culture, glass coverslips (25 mm, # 1 Menzel Gläser) were coated in 0.0008 % (w/v) Poly-L-Lysine (Sigma-Aldrich, P4707) in phosphate-buffered-saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... For transfection and patch-clamp experiments cells were plated at density 25,000 cells per 13-mm-diameter coverslip (Assistent, Germany) coated with poly-L-Lysine (Sigma-Aldrich).Cells were co-transfected with plasmids coding GLT 1 a or GLT 1 a-SEP under CMV promoter together with mRFP1 under β-actin promoter in a 2:1 ratio using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... astrocytes were resuspended in supplemented DMEM and seeded on cell culture plates previously coated with 0.2 mg/ml poly-L-lysine (Sigma-Aldrich) in Milli-Q ...
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bioRxiv - Neuroscience 2020Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid (Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... The next day cells were washed three times with PBS and cultured with DMEM (DU145 cells) or RPMI (LNCaP cells) medium supplemented with 2 mM [U-13C5] L-glutamine (Sigma) for 10 ...
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bioRxiv - Biophysics 2021Quote: ... the glass coverslips were coated with 50 μg ml−1 of poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The guts were then transferred in a droplet to #1.5 coverslips coated with poly-l-lysine (P4832-50ML, Sigma Aldrich) with 120 µm spacers (620001 ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Cell Biology 2021Quote: Hek293T cells were plated in 24-well plates on cover glass (Carl Roth) coated with poly-L- Lysine (Sigma-Aldrich). Cells were transfected with 125 ng of plasmid and total plasmid levels were adjusted to 500 ng with empty plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were incubated with Coomassie stain (60mg/l Coomassie Blue R-250, 10% v/v acetic acid, both Sigma, UK) Following 24h de-staining in 10% v/v acetic acid ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated with secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-human Fab IgG (H+L) (Sigma, A0293) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Cell Biology 2021Quote: ... Hearts were removed by lateral thoracotomy and directly immersed in ice-cold HBSS supplemented with 20 mmol/l 2,3-Butanedione monoxime (BDM, Sigma-Aldrich) unless stated otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... microvascular cells were cultured at 37°C with 5% CO2 on fibronectin-coated culture flasks in SupplementPack Endothelial Cell GM2 containing 0.01 mL/mL L-Glutamine (Sigma, #G3202), 0.02 mL/mL Fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell pellets were resuspended into BMM-C to an OD600 of approximately 1 and then inoculated into BMM-levan (5 g L-1 levan, Sigma) using an initial OD600 of 0.05 in a 96-well plate (Greiner Bio-One) ...
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bioRxiv - Plant Biology 2020Quote: ... the emergent hairy roots could be distinguished from wild type roots by their branching phenotype and were sub-cultured on RGM_3xSuc medium (Table 2) with added 25 mg/L meropenem (Sigma Aldrich). The hairy roots were sub-cultured every 4 weeks on the same medium and were cultured in the dark at 25°C.
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bioRxiv - Synthetic Biology 2021Quote: ... The natural transformation was started by adding 3.5 µL of the precultures (OD600 ∼ 9 - 11) to 350 µL sea salt medium (28 g/L (Sigma, S9883)) with 100 µM IPTG in a 1.5 mL reaction tube ...