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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 4-20% gradient SDS-PAGE gel (Cosmobio, 414879) and then transferred to PVDF membrane (Millipore, IPVH00010). To detect CAMSAP3 and GAPDH for a loading control ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on a gel were transferred to a nitrocellulose membrane and analyzed by using the antibodies at 1:3000-10,000 dilutions: DM1a (Sigma, #T9026), α-GFP (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... subjected to sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS–PAGE) and electro transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Germany). The membranes were then blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2021Quote: ... Frozen sections were used to visualize cardiomyocyte cell membranes by staining with FITC-conjugated wheat-germ agglutinin (Sigma-Aldrich, USA), as described before Zhao et al ...
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bioRxiv - Microbiology 2020Quote: Schleicher & Schuell Minifold II Slot Blot System SRC072/0 44-27570 Manifold was used for transferring samples from sucrose gradient fractions to PVDF membranes (Immobilon PSQ, Merk Millipore). Shortly ...
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bioRxiv - Developmental Biology 2021Quote: ... Followed by covering them in halocarbon oil 27 and sandwiched between the membrane and the cover glass slide (cover glass washed and cleaned with pure ethanol, slide #1.5, Sigma BR470045). The data collection was performed using a custom-built two-photon microscope using 970 nm laser excitation for green (EGFP and mNeonGreen tagged protein and MS2 loops respectively ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Biochemistry 2021Quote: ... After blocking the membrane with 2 % non-fat dry milk dissolved in PBS + 0.1 % (v/v) tween-20 (PBST; Sigma-Aldrich), membranes were incubated with primary antibodies for either 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with primary antibodies specific for TSC22D4 (home-made, by PINEDA Antibody Service, Flag-HRP (#A8592, Sigma), HA (#2999 ...
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bioRxiv - Cancer Biology 2021Quote: 0.08 mg of Cy5.5 in 100 μL water was mixed with 0.2 mg of PLL in 1 mM 100 μL NaHCO3 in the dark at room temperature for 30 min (vortexed every 10 min) and filtered through molecular-weight cutoff membrane filters (10 kDa, Millipore). The resulting PLL-Cy5.5 was collected and washed several times with sterilized water until the color of the filtrate was clear ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates were separated by SDS-gel electrophoresis (15% polyacrylamide for Arfs) and transferred to Immobilon-P PDVF membranes (Millipore). Membranes were blocked with TBST (20 mM Tris ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then solubilized overnight at 4°C in presence of 0.5% n-Dodecyl β-D-maltoside (DDM) (Sigma, D4641). Lysates were used for co-immunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... Equal amounts of protein were separated by 10% SDS-PAGE gel and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were incubated with RCC2 (CST ...
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bioRxiv - Microbiology 2020Quote: ... Water and biofilms from the outflow were collected in a sterile 1 L bottle and filtered through a 0.22 μm nitrocellulose membrane (Millipore, USA). The membrane was transported to the laboratory on ice and stored at 4 °C for bacterial enrichment into 10 mL of Nutrient Broth (Biolife ...
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bioRxiv - Microbiology 2020Quote: ... The PVDF membrane was blocked for 1 h in PBS supplemented with 0.1 % Tween-80 from Sigma Aldrich (‘PBS-Tween’) and 4 % of milk powder ...
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bioRxiv - Immunology 2021Quote: ... Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size, 30 mm diameter, 2-3 slices per insert; Millipore Corp) in six well tissue culture treated plates containing MEM with 25% horse serum and 6.5 mg/mL of glucose ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM β-mercaptoethanol and 5% glycerol with minimum of three changes in buffer and concentrated using membrane concentrators (10-kDa MWCO; Millipore). The purified PfGet3-6×his was subsequently used for the custom generation of anti-PfGet3 antibodies or for in vitro binding experiments.
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bioRxiv - Microbiology 2021Quote: Samples containing equal amounts of protein were separated using sodium dodecyl sulfate (SDS)-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore). After blocking with 5% skim milk in TBST for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.75 μl of egg extract was subjected to SDS-PAGE, transferred to Protran nitrocellulose (Protran, Amersham) or Immobilon membranes (Millipore) and upon blocking using TBST /5% milk or TBST/5% BSA (when phospho-antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins from whole cell lysates (20–60 µg) were separated on 8-10-12% polyacrylamide gels and transferred to Immobilon-FL membranes (Millipore). Primary and secondary antibodies were diluted in blocking buffer (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked for 1 hour at RT using 5% milk in TBS with 0.025% Tween 20 (Sigma, P1379) (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting multilamellar liposomes were extruded 11 times through two stacked polycarbonate membranes of 0.8 μm pore diameter (Whatman-Nuclepore, Sigma-Aldrich) using a custom-built high-pressure extrusion device or a syringe extruder (Avanti Polar Lipids). ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed and incubated with primary antibody diluted in 5% BSA/TBST (1:10,000 anti-Flag (Sigma-Aldrich) or anti-Beta lactamase (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 50 µl LB and deposited on 22 mm filter membranes with 0.45 μm pores (Millipore, Merck) on a pre-dried LB agar plate ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Immunology 2022Quote: ... 10-15 µg of proteins were loaded onto a 96-well plate with an activated and primed polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma). Proteins adsorbed to the membrane were trypsinized by incubation with the protease for 2 hours at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578 ...
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bioRxiv - Developmental Biology 2022Quote: ... For Western blot analysis the proteins were electrophoretically separated on a 10 % polyacrylamide gel and transferred onto a polyvinyldifluoride membrane (PVDF, Millipore). The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μg of each fraction was separated by SDS-PAGE and transferred to 0.45 μm Polyvinylidene fluoride (PVDF) membranes (Immobilon-P, EMD Millipore).
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (lysate or immunoprecipitation samples) were separated by SDS-PAGE and transferred to 0.45 μm Polyvinylidene fluoride (PVDF) membranes (Immobilon-P, EMD Millipore). For affinity purification of endogenous or epitope-tagged binding proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... were loaded onto a 10% or 12.5% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane (PVDF; Millipore-Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Fraction 5/6 (found at the interface between 22.5% and 9.5% Nycodenz and enriched in ER and autophagosome membranes (Strømhaug et al., 1998)) was diluted 1.25X and loaded on a Percoll (Sigma-Aldrich; P4937)/Nycodenz gradient (110 μL of 22.5% Nycodenz on bottom ...
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bioRxiv - Cell Biology 2022Quote: ... liposomes were extruded with the LipSoFast-Basic extruder (Avestin) 21 times through 2 polycarbonate membranes (Sigma-Aldrich; WHA10417004 or WHA110405). For testing SMA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were then subjected to SDS-PAGE (10%) and visualized by silver staining (Pierce) or transferred to PVDF membranes (Millipore). The membrane was blocked in TBS/Tween 0.1% (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... The SDS samples were subjected to SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to Immobilon-P membranes (Millipore, IPVH00010). The membranes were blocked with 5% skim milk (Yukijirushi ...
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bioRxiv - Cell Biology 2022Quote: ... Enhanced chemiluminescence (ECL) signal was generated by incubating the membranes with Immobilon Forte Western HRP Substrate (Millipore. Cat. Nº: WBLUF0100). ECL and fluorescent emission were digitalized with the iBright imaging system (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... mitochondria enriched heavy membrane fractions (200 μg) from control fibroblasts were chemically cross-linked with 1 mM dithiobis-sulfosuccinimidyl propionate (DSP) (Sigma) in HIM buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated by 8–15% sodium dodecyl sulfate-polyacrylamide gels and transferred to a PVDF membrane (IPVH00010 Millipore Immobilon). After transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE in 5-20% gradient gels (Extra PAGE One Precast Gel, Nacalai Tesque) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). After blocking with 5% skim milk (Nacalai Tesque ...